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SG10201807524YA - A multi-index detection microfluidic chip and methods of use - Google Patents

A multi-index detection microfluidic chip and methods of use

Info

Publication number
SG10201807524YA
SG10201807524YA SG10201807524YA SG10201807524YA SG10201807524YA SG 10201807524Y A SG10201807524Y A SG 10201807524YA SG 10201807524Y A SG10201807524Y A SG 10201807524YA SG 10201807524Y A SG10201807524Y A SG 10201807524YA SG 10201807524Y A SG10201807524Y A SG 10201807524YA
Authority
SG
Singapore
Prior art keywords
bottom plate
hole
microfluidic chip
detection
main channel
Prior art date
Application number
SG10201807524YA
Inventor
Lei Wang
Guohao Zhang
Xinying Zhou
Juan Xin
Yao Zhang
Mingxian Lin
Guoliang Huang
Can Wang
Wanli Xing
Jing Cheng
Original Assignee
Capitalbio Corp
Univ Tsinghua
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Capitalbio Corp, Univ Tsinghua filed Critical Capitalbio Corp
Publication of SG10201807524YA publication Critical patent/SG10201807524YA/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Micromachines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Optical Measuring Cells (AREA)

Abstract

A MULTI-INDEX DETECTION MICROFLUIDIC CHIP AND METHODS OF USE A multi-index detection micro fluidic chip is provided. A bottom plate () and a cover plate (2) that matches the bottom plate (1) and seals it are provided in the micro fluidic chip. The center of the microfluidic chip has a through-hole (3). The bottom plate (1) has one or more wave-shaped main channel (s) (4), one end of each of the main channel (4) connected to a sample injection hole (9) on the bottom plate (1), and the other end connected to an exhaust hole (10) on the bottom plate (1). The valley on the main channel (1) is distal to the through-hole (3), and the peak is proximal to the through-hole (3). Each valley of the main channel (1) is connected to a reaction chamber (6) by a linking channel (5). The linking channel (5) comprises one or more buffering chambers (7). The microfluidic chip can be used in detection by fluorescence, turbidity, color, detection equipment, and/or direct observation by the naked eyes. The detection is real-time as the reaction occurs or after the reaction. Figure 1 45
SG10201807524YA 2014-03-07 2015-03-06 A multi-index detection microfluidic chip and methods of use SG10201807524YA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410082166.2A CN103831140B (en) 2014-03-07 2014-03-07 A kind of micro-fluidic chip of multiple determination

Publications (1)

Publication Number Publication Date
SG10201807524YA true SG10201807524YA (en) 2018-09-27

Family

ID=50795220

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201807524YA SG10201807524YA (en) 2014-03-07 2015-03-06 A multi-index detection microfluidic chip and methods of use
SG11201607270PA SG11201607270PA (en) 2014-03-07 2015-03-06 A multi-index detection microfluidic chip and methods of use

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201607270PA SG11201607270PA (en) 2014-03-07 2015-03-06 A multi-index detection microfluidic chip and methods of use

Country Status (6)

Country Link
US (1) US10300483B2 (en)
EP (1) EP3113886B1 (en)
JP (1) JP6754701B2 (en)
CN (1) CN103831140B (en)
SG (2) SG10201807524YA (en)
WO (1) WO2015131662A1 (en)

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Also Published As

Publication number Publication date
US20170014818A1 (en) 2017-01-19
SG11201607270PA (en) 2016-09-29
EP3113886A4 (en) 2017-10-25
CN103831140A (en) 2014-06-04
JP6754701B2 (en) 2020-09-16
JP2017514686A (en) 2017-06-08
CN103831140B (en) 2015-12-30
US10300483B2 (en) 2019-05-28
EP3113886A1 (en) 2017-01-11
WO2015131662A1 (en) 2015-09-11
EP3113886B1 (en) 2019-10-23

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