[go: up one dir, main page]

SE9903336D0 - DNA construct and its use - Google Patents

DNA construct and its use

Info

Publication number
SE9903336D0
SE9903336D0 SE9903336A SE9903336A SE9903336D0 SE 9903336 D0 SE9903336 D0 SE 9903336D0 SE 9903336 A SE9903336 A SE 9903336A SE 9903336 A SE9903336 A SE 9903336A SE 9903336 D0 SE9903336 D0 SE 9903336D0
Authority
SE
Sweden
Prior art keywords
oilseed plant
peptide
dna construct
nucleotide sequence
sequence coding
Prior art date
Application number
SE9903336A
Other languages
Swedish (sv)
Inventor
Anna-Stina Hoeglund
Kjell Staalberg
Original Assignee
Astacarotene Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Astacarotene Ab filed Critical Astacarotene Ab
Priority to SE9903336A priority Critical patent/SE9903336D0/en
Publication of SE9903336D0 publication Critical patent/SE9903336D0/en
Priority to CA002382444A priority patent/CA2382444A1/en
Priority to PCT/SE2000/001767 priority patent/WO2001020011A1/en
Priority to EP00966616A priority patent/EP1212444A1/en
Priority to AU76936/00A priority patent/AU7693600A/en
Priority to JP2001523782A priority patent/JP2003509057A/en
Priority to NO20021305A priority patent/NO20021305L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

A DNA construct comprising in the 5' to 3' direction of transcription operably linked a promoter region directing transcription to the seed of an oilseed plant, a nucleotide sequence coding for at least one peptide with enzyme activity necessary for keto group containing xanthophyll production and esterification in an oilseed plant and a transcriptional termination region is disclosed. The DNA construct may additionally comprise a nucleotide sequence coding for a transit peptide directing the translated fusion polypeptide to the chloroplast of the oilseed plant. The peptide with enzyme activity is preferably a peptide with beta -carotene C-4-oxygenase activity, e.g. from the alga <i><aematococcus pluvialis</i>. Comprised by the invention are also a transgenic oilseed plant cell, e.g. of rape, sunflower, soybean or mustard origin, and a transgenic oilseed plant-produced xanthophyll, such as canthaxanthin or astaxanthin, and also astaxanthin esters.
SE9903336A 1999-09-17 1999-09-17 DNA construct and its use SE9903336D0 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
SE9903336A SE9903336D0 (en) 1999-09-17 1999-09-17 DNA construct and its use
CA002382444A CA2382444A1 (en) 1999-09-17 2000-09-13 Dna construct and its use
PCT/SE2000/001767 WO2001020011A1 (en) 1999-09-17 2000-09-13 Dna construct and its use
EP00966616A EP1212444A1 (en) 1999-09-17 2000-09-13 Dna construct and its use
AU76936/00A AU7693600A (en) 1999-09-17 2000-09-13 Dna construct and its use
JP2001523782A JP2003509057A (en) 1999-09-17 2000-09-13 DNA constructs and uses thereof
NO20021305A NO20021305L (en) 1999-09-17 2002-03-15 DNA construction and its use

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9903336A SE9903336D0 (en) 1999-09-17 1999-09-17 DNA construct and its use

Publications (1)

Publication Number Publication Date
SE9903336D0 true SE9903336D0 (en) 1999-09-17

Family

ID=20417035

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9903336A SE9903336D0 (en) 1999-09-17 1999-09-17 DNA construct and its use

Country Status (7)

Country Link
EP (1) EP1212444A1 (en)
JP (1) JP2003509057A (en)
AU (1) AU7693600A (en)
CA (1) CA2382444A1 (en)
NO (1) NO20021305L (en)
SE (1) SE9903336D0 (en)
WO (1) WO2001020011A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7227057B2 (en) 1997-06-03 2007-06-05 Chromatin, Inc. Plant centromere compositions
US7235716B2 (en) 1997-06-03 2007-06-26 Chromatin, Inc. Plant centromere compositions
US7193128B2 (en) 1997-06-03 2007-03-20 Chromatin, Inc. Methods for generating or increasing revenues from crops
US7119250B2 (en) 1997-06-03 2006-10-10 The University Of Chicago Plant centromere compositions
EP1303614A2 (en) 2000-07-18 2003-04-23 National Research Council Of Canada Cloning, sequencing and expression of a comamonas cyclopentanone 1,2-monooxygenase-encoding gene in escherichia coli
EP1532265A2 (en) 2002-08-20 2005-05-25 Sungene GmbH & Co. KGaA Method for producing ketocarotinoids in genetically modified organisms
DE10321963A1 (en) * 2003-05-15 2004-12-02 Icon Genetics Ag Process for the production of a plastid-directed protein in plant cells
AU2006227165B2 (en) 2005-03-18 2011-11-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US20070293568A1 (en) 2006-06-16 2007-12-20 Yamaha Hatsudoki Kabushiki Kaisha Neurocyte Protective Agent
WO2008042338A2 (en) 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
AU2010275448B2 (en) 2009-07-23 2014-03-06 Chromatin, Inc. Sorghum centromere sequences and minichromosomes

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5618988A (en) * 1990-03-02 1997-04-08 Amoco Corporation Enhanced carotenoid accumulation in storage organs of genetically engineered plants
US5916791A (en) * 1995-11-24 1999-06-29 Hirschberg; Joseph Polynucleotide molecule from Haematococcus pluvialis encoding a polypeptide having a β--C--4--oxygenase activity for biotechnological production of (3S,3S)astaxanthin
WO1998006862A1 (en) * 1996-08-09 1998-02-19 Calgene Llc Methods for producing carotenoid compounds and speciality oils in plant seeds
US6429356B1 (en) * 1996-08-09 2002-08-06 Calgene Llc Methods for producing carotenoid compounds, and specialty oils in plant seeds
SE522246C2 (en) * 1997-02-27 2004-01-27 Astacarotene Ab Oral preparation for prophylactic and therapeutic treatment of Helicobacter Sp. infection
SE511237C2 (en) * 1997-12-16 1999-08-30 Astacarotene Ab Use of at least one type of xanthophyll for the preparation of a human or veterinary drug for the prophylactic treatment of mastitis in mammalian mothers

Also Published As

Publication number Publication date
WO2001020011A1 (en) 2001-03-22
CA2382444A1 (en) 2001-03-22
JP2003509057A (en) 2003-03-11
NO20021305D0 (en) 2002-03-15
AU7693600A (en) 2001-04-17
NO20021305L (en) 2002-05-15
EP1212444A1 (en) 2002-06-12

Similar Documents

Publication Publication Date Title
SE9903336D0 (en) DNA construct and its use
WO1994010288A3 (en) Medium-chain thioesterases in plants
NO971945D0 (en) Increased carotenoid accumulation in the storage organs of plants produced by genetic engineering
CA2193094A1 (en) The cytoplasmic inhibition of gene expression
CO5021164A1 (en) GENES OF ELONGASA AND ITS USES
PE20001561A1 (en) GENES AND VECTORS TO CONFER RESISTANCE TO HERBICIDES IN PLANTS
WO2003011015A3 (en) Methods for improving seed and grain characteristics
CA2257198A1 (en) Suppression of specific classes of soybean seed protein genes
WO1996021022A3 (en) Production of gamma linolenic acid by a δ6-desaturase
FI962633A (en) DNA strands useful in the synthesis of xanthophyll and method for preparing xanthophyll
DK1097211T3 (en) Expression of eukaryotic peptides in plant plastids
WO1999029875A3 (en) Plant uridine diphosphate-glucose dehydrogenase genes, proteins, and uses thereof
BR0011400A (en) Isolated nucleic acid sequence, amino acid sequence, expression cassette, vector, organism, transgenic plant, processes for preparing unsaturated fatty acids, and for preparing triglycerides, protein, unsaturated fatty acid, triglyceride, and, using a nucleic acid sequence , unsaturated fatty acids or triglycerides with an increased content of unsaturated fatty acids, and proteins
Ritala et al. Production of a recombinant industrial protein using barley cell cultures
AR075363A2 (en) PROMOTERS WITH PREFERENCE FOR CORN ROOTS AND USES OF THE SAME
Sheldon et al. 3-Oxoacyl-[ACP] reductase from oilseed rape (Brassica napus)
CN111575310B (en) Recombinant saccharomyces cerevisiae expressing caveolin and its application
AU694098B2 (en) DNA encoding 2-acyltransferases
DE69940103D1 (en) Farnesyl pyrophosphate synthase (FPS) from seedlings of the sunflower (Helianthus annus)
Terpstra Identification of chlorophyllase as a glycoprotein
AR020332A1 (en) METHOD FOR INCREASING THE PRODUCTION OF A PROTEIN IN A PLANT CELL AND A CONSTRUCTION
BR0009671A (en) Protein, nucleic acid, construction of nucleic acid, genetically modified organism, process to produce genetically manipulated organisms, use of nucleic acid, process for the preparation of xanthophyll derivatives, and, use of protein
Liu et al. Enhancing carbon dioxide fixation and co-production of protein and lutein in oleaginous Coccomyxa subellipsoidea by a stepwise light intensity and nutrients feeding strategy
Breitenbach et al. Carotenoid synthesis and phytoene synthase activity during mating of Blakeslea trispora
GB2335195A (en) Transcriptional regulation in plants