SE9300090D0 - AFFINITY CLEANING WITH COMPLEX BOND LIGAND - Google Patents
AFFINITY CLEANING WITH COMPLEX BOND LIGANDInfo
- Publication number
- SE9300090D0 SE9300090D0 SE9300090A SE9300090A SE9300090D0 SE 9300090 D0 SE9300090 D0 SE 9300090D0 SE 9300090 A SE9300090 A SE 9300090A SE 9300090 A SE9300090 A SE 9300090A SE 9300090 D0 SE9300090 D0 SE 9300090D0
- Authority
- SE
- Sweden
- Prior art keywords
- target substance
- ligand
- matrix
- affinity
- binding sites
- Prior art date
Links
- 239000003446 ligand Substances 0.000 title abstract 5
- 238000004140 cleaning Methods 0.000 title 1
- 239000013076 target substance Substances 0.000 abstract 10
- 239000011159 matrix material Substances 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 3
- 238000002955 isolation Methods 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Peptides Or Proteins (AREA)
Abstract
Method for affinity based isolation of a first target substance, said method comprising: (i) contacting said first target substance with a matrix to which is linked a ligand having affinity for said first target substance; (ii) eluting said first target substance. The method is characterized by the ligand having been initially complexed/bound to a second target substance having weaker binding ability to the ligand than the first target substance and providing multi-point attachment to the matrix by carrying several ligand binding sites and/or other matrix binding sites on its molecule enabling adsorption of the first target substance during step (i) without the second target substance becoming eluted from the matrix. In one mode the second target substance is thermo-reactive, and steps (i) and (ii) are run at different temperatures separated by the cloud point of the second target substance.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9300090A SE9300090D0 (en) | 1993-01-14 | 1993-01-14 | AFFINITY CLEANING WITH COMPLEX BOND LIGAND |
| PCT/SE1994/000016 WO1994015951A1 (en) | 1993-01-14 | 1994-01-13 | Affinity purification by the use of a complexed ligand |
| AU58942/94A AU5894294A (en) | 1993-01-14 | 1994-01-13 | Affinity purification by the use of a complexed ligand |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9300090A SE9300090D0 (en) | 1993-01-14 | 1993-01-14 | AFFINITY CLEANING WITH COMPLEX BOND LIGAND |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SE9300090D0 true SE9300090D0 (en) | 1993-01-14 |
Family
ID=20388535
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE9300090A SE9300090D0 (en) | 1993-01-14 | 1993-01-14 | AFFINITY CLEANING WITH COMPLEX BOND LIGAND |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU5894294A (en) |
| SE (1) | SE9300090D0 (en) |
| WO (1) | WO1994015951A1 (en) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0835446B1 (en) | 1995-06-26 | 2003-04-09 | Perseptive Biosystems, Inc. | High speed, automated, continuous flow, multi-dimensional molecular selection and analysis |
| EP1300679A3 (en) * | 1995-06-26 | 2004-04-14 | Perseptive Biosystems, Inc. | Molecular selection and analysis |
| AU4612400A (en) * | 1999-05-11 | 2000-11-21 | Japan Chemical Innovation Institute | Affinity-controlling material with the use of stimulus-responsive polymer and separation/purification method with the use of the material |
| ES2545526T3 (en) | 2001-02-01 | 2015-09-11 | Sigma-Aldrich Co. Llc | Enhanced affinity matrices with enhanced visibility for molecular drag and immunoprecipitation applications |
| FR2902799B1 (en) | 2006-06-27 | 2012-10-26 | Millipore Corp | METHOD AND UNIT FOR PREPARING A SAMPLE FOR THE MICROBIOLOGICAL ANALYSIS OF A LIQUID |
| US20100267933A1 (en) | 2006-12-21 | 2010-10-21 | Moya Wilson | Purification of proteins |
| US8569464B2 (en) | 2006-12-21 | 2013-10-29 | Emd Millipore Corporation | Purification of proteins |
| US8362217B2 (en) | 2006-12-21 | 2013-01-29 | Emd Millipore Corporation | Purification of proteins |
| WO2009151514A1 (en) | 2008-06-11 | 2009-12-17 | Millipore Corporation | Stirred tank bioreactor |
| ES2749232T3 (en) | 2008-12-16 | 2020-03-19 | Emd Millipore Corp | Stirred tank reactor and procedure |
| CN107312062B (en) | 2010-05-17 | 2021-03-16 | Emd密理博公司 | Stimulus responsive polymers for purification of biomolecules |
| CN118914420B (en) * | 2024-07-19 | 2025-01-28 | 南京鼓楼医院 | A method for pre-treatment of plasma protein by mass spectrometry |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0056254A1 (en) * | 1981-01-14 | 1982-07-21 | David Eldon Wood | Treatment of insoluble surfaces to inhibit nonspecific protein binding |
| GB8317855D0 (en) * | 1983-06-30 | 1983-08-03 | Iq Bio Ltd | Biochemical detection method |
-
1993
- 1993-01-14 SE SE9300090A patent/SE9300090D0/en unknown
-
1994
- 1994-01-13 AU AU58942/94A patent/AU5894294A/en not_active Abandoned
- 1994-01-13 WO PCT/SE1994/000016 patent/WO1994015951A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| AU5894294A (en) | 1994-08-15 |
| WO1994015951A1 (en) | 1994-07-21 |
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