SE8803528D0 - CLONING AND EXPRESSION OF TRANSFORMING GROWTH FACTOR BETA2 - Google Patents
CLONING AND EXPRESSION OF TRANSFORMING GROWTH FACTOR BETA2Info
- Publication number
- SE8803528D0 SE8803528D0 SE8803528A SE8803528A SE8803528D0 SE 8803528 D0 SE8803528 D0 SE 8803528D0 SE 8803528 A SE8803528 A SE 8803528A SE 8803528 A SE8803528 A SE 8803528A SE 8803528 D0 SE8803528 D0 SE 8803528D0
- Authority
- SE
- Sweden
- Prior art keywords
- beta
- tgf
- mature
- cells
- tamoxifen
- Prior art date
Links
- 102000011117 Transforming Growth Factor beta2 Human genes 0.000 title 1
- 101800000304 Transforming growth factor beta-2 Proteins 0.000 title 1
- 238000010367 cloning Methods 0.000 title 1
- 229940072041 transforming growth factor beta 2 Drugs 0.000 title 1
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 abstract 11
- NKANXQFJJICGDU-QPLCGJKRSA-N Tamoxifen Chemical compound C=1C=CC=CC=1C(/CC)=C(C=1C=CC(OCCN(C)C)=CC=1)/C1=CC=CC=C1 NKANXQFJJICGDU-QPLCGJKRSA-N 0.000 abstract 4
- 210000004027 cell Anatomy 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 150000001413 amino acids Chemical group 0.000 abstract 2
- 210000004978 chinese hamster ovary cell Anatomy 0.000 abstract 2
- 239000002299 complementary DNA Substances 0.000 abstract 2
- 239000002243 precursor Substances 0.000 abstract 2
- 229960001603 tamoxifen Drugs 0.000 abstract 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 1
- 241000282552 Chlorocebus aethiops Species 0.000 abstract 1
- 108020004635 Complementary DNA Proteins 0.000 abstract 1
- 101500025614 Homo sapiens Transforming growth factor beta-1 Proteins 0.000 abstract 1
- 101500025624 Homo sapiens Transforming growth factor beta-2 Proteins 0.000 abstract 1
- 238000000636 Northern blotting Methods 0.000 abstract 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
- 239000002751 oligonucleotide probe Substances 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 230000001124 posttranscriptional effect Effects 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 201000005825 prostate adenocarcinoma Diseases 0.000 abstract 1
- 230000006337 proteolytic cleavage Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/495—Transforming growth factor [TGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
cDNA clones coding for TGF- beta 2 which are used to construct expression vectors capable of directing the high-level expression of mature, biologically active TGF- beta 2 in transfected Chinese Hamster Ovary cells (CHO cells) are described. The TGF- beta 2 produced and secreted by CHO transfectants has a specific activity estimated to be about half that of recombinant TGF- beta 1. The clones were isolated from a tamoxifen-treated human prostatic adenocarcinoma cell line (PC-3) using oligonucleotide probes based on the partial amino acid sequence of purified TGF- beta 2 from an African green monkey cell line, BSC-40. The cDNA sequences predict that TGF- beta 2 is synthesized as polypeptide precursor forms from which the mature 112 amino acid TGF- beta 2 subunit is derived by proteolytic cleavage. The proteins coded for by the human TGF- beta 1 and TGF- beta 2 cDNAs show an overall homology of 41%. The mature and amino- terminal precursor regions show 71% and 31% homology respectively. Northern blot analysis identified TGF- beta 2 transcripts of 4.1 kb, 5.1 kb, and 6.5 kb using mRNA from several different sources. Analysis of polyadenylated RNA from tamoxifen-treated PC-3 cells showed that these cells contain higher amounts of TGF- beta 1 transcripts than TGF- beta 2, although they produce more TGF- beta 2 protein than TDF- beta 1. This suggests that there is a post-transcriptional level of regulation for the production of these proteins.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10675287A | 1987-10-06 | 1987-10-06 | |
| US14826788A | 1988-01-25 | 1988-01-25 | |
| US23406588A | 1988-08-18 | 1988-08-18 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE8803528D0 true SE8803528D0 (en) | 1988-10-05 |
| SE8803528L SE8803528L (en) | 1989-04-07 |
Family
ID=27380183
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8803528A SE8803528L (en) | 1987-10-06 | 1988-10-05 | CLONING AND EXPRESSION OF TRANSFORMATION GROWTH FACTOR BETA2 |
Country Status (22)
| Country | Link |
|---|---|
| JP (1) | JPH02444A (en) |
| KR (1) | KR970001236B1 (en) |
| CN (1) | CN1035845A (en) |
| AT (1) | AT398433B (en) |
| BE (1) | BE1002693A5 (en) |
| CH (1) | CH680002A5 (en) |
| DE (1) | DE3833897C2 (en) |
| DK (1) | DK556388A (en) |
| ES (1) | ES2012556A6 (en) |
| FI (1) | FI884546A7 (en) |
| FR (1) | FR2621324B1 (en) |
| GB (1) | GB2210620B8 (en) |
| GR (1) | GR1000492B (en) |
| IE (1) | IE60918B1 (en) |
| IL (1) | IL87877A0 (en) |
| IT (1) | IT1227957B (en) |
| MY (1) | MY103779A (en) |
| NL (1) | NL8802442A (en) |
| NO (1) | NO884429L (en) |
| NZ (1) | NZ226486A (en) |
| PT (1) | PT88670B (en) |
| SE (1) | SE8803528L (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5221620A (en) * | 1987-10-06 | 1993-06-22 | Oncogen | Cloning and expression of transforming growth factor β2 |
| US5445941A (en) * | 1993-06-21 | 1995-08-29 | Eli Lilly And Company | Method for screening anti-osteoporosis agents |
| CN100389120C (en) * | 2005-04-12 | 2008-05-21 | 中国人民解放军第三军医大学野战外科研究所 | Sealing TGF-betal antisensedingonucleotides and its use in preparing medicine for treating fibrillation related disease |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE128715T1 (en) * | 1984-07-16 | 1995-10-15 | Celtrix Pharma | POLYPEPTIDE-INDUCING FACTORS IN BONE AND CARTILAGE. |
| IL78197A (en) * | 1985-03-22 | 1991-07-18 | Genentech Inc | Nucleic acid encoding tgf-beta and its uses |
| FI883363L (en) * | 1986-11-17 | 1988-07-14 | Sandoz Ag | FRAMSTAELLNING AV EN T-CELLSUPRESSORFAKTOR OCH DESS ANVAENDNING. |
| AU1340688A (en) * | 1987-01-30 | 1988-08-24 | Techne Corporation | Transforming growth factor-beta |
| US4931548A (en) * | 1987-01-30 | 1990-06-05 | Techne Corporation | Heterodimer form of transforming growth factor-beta |
| AU1539188A (en) * | 1987-05-04 | 1988-11-10 | Bristol-Myers Squibb Company | TGF-B2 and novel compositions having anti-neoplastic activity |
-
1988
- 1988-09-29 IL IL87877A patent/IL87877A0/en active Protection Beyond IP Right Term
- 1988-10-03 FI FI884546A patent/FI884546A7/en not_active IP Right Cessation
- 1988-10-04 MY MYPI88001108A patent/MY103779A/en unknown
- 1988-10-04 PT PT88670A patent/PT88670B/en not_active IP Right Cessation
- 1988-10-05 DE DE3833897A patent/DE3833897C2/en not_active Expired - Fee Related
- 1988-10-05 NO NO88884429A patent/NO884429L/en unknown
- 1988-10-05 KR KR1019880012986A patent/KR970001236B1/en not_active Expired - Lifetime
- 1988-10-05 CN CN88109075A patent/CN1035845A/en active Pending
- 1988-10-05 IE IE301888A patent/IE60918B1/en not_active IP Right Cessation
- 1988-10-05 DK DK556388A patent/DK556388A/en not_active Application Discontinuation
- 1988-10-05 BE BE8801141A patent/BE1002693A5/en not_active IP Right Cessation
- 1988-10-05 SE SE8803528A patent/SE8803528L/en not_active Application Discontinuation
- 1988-10-05 NL NL8802442A patent/NL8802442A/en not_active Application Discontinuation
- 1988-10-05 FR FR8813046A patent/FR2621324B1/en not_active Expired - Fee Related
- 1988-10-05 ES ES8803023A patent/ES2012556A6/en not_active Expired - Lifetime
- 1988-10-05 GR GR880100660A patent/GR1000492B/en unknown
- 1988-10-05 AT AT0245488A patent/AT398433B/en not_active IP Right Cessation
- 1988-10-05 CH CH3702/88A patent/CH680002A5/fr not_active IP Right Cessation
- 1988-10-06 GB GB8823448A patent/GB2210620B8/en not_active Expired - Fee Related
- 1988-10-06 NZ NZ226486A patent/NZ226486A/en unknown
- 1988-10-06 JP JP63251011A patent/JPH02444A/en active Pending
- 1988-10-06 IT IT8822213A patent/IT1227957B/en active
Also Published As
| Publication number | Publication date |
|---|---|
| DE3833897C2 (en) | 1997-03-20 |
| DK556388D0 (en) | 1988-10-05 |
| NL8802442A (en) | 1989-05-01 |
| CN1035845A (en) | 1989-09-27 |
| JPH02444A (en) | 1990-01-05 |
| NO884429L (en) | 1989-04-07 |
| IT8822213A0 (en) | 1988-10-06 |
| GR1000492B (en) | 1992-07-30 |
| IE883018L (en) | 1989-04-06 |
| FI884546A7 (en) | 1989-04-07 |
| CH680002A5 (en) | 1992-05-29 |
| DK556388A (en) | 1989-04-07 |
| MY103779A (en) | 1993-09-30 |
| IL87877A0 (en) | 1989-03-31 |
| IT1227957B (en) | 1991-05-20 |
| GB2210620B8 (en) | 1995-07-17 |
| PT88670B (en) | 1992-12-31 |
| GB2210620A (en) | 1989-06-14 |
| KR970001236B1 (en) | 1997-02-04 |
| SE8803528L (en) | 1989-04-07 |
| FI884546A0 (en) | 1988-10-03 |
| BE1002693A5 (en) | 1991-05-07 |
| GB8823448D0 (en) | 1988-11-16 |
| FR2621324B1 (en) | 1994-02-18 |
| ES2012556A6 (en) | 1990-04-01 |
| IE60918B1 (en) | 1994-08-24 |
| DE3833897A1 (en) | 1989-05-03 |
| FR2621324A1 (en) | 1989-04-07 |
| NO884429D0 (en) | 1988-10-05 |
| GB2210620B (en) | 1992-07-08 |
| KR890006814A (en) | 1989-06-16 |
| AT398433B (en) | 1994-12-27 |
| ATA245488A (en) | 1994-04-15 |
| NZ226486A (en) | 1991-08-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| NAV | Patent application has lapsed |
Ref document number: 8803528-2 |