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SE8007396L - PROCEDURES AND METHODS FOR DETERMINING A LIGAND IN A SCIENTIFIC MEDIUM - Google Patents

PROCEDURES AND METHODS FOR DETERMINING A LIGAND IN A SCIENTIFIC MEDIUM

Info

Publication number
SE8007396L
SE8007396L SE8007396A SE8007396A SE8007396L SE 8007396 L SE8007396 L SE 8007396L SE 8007396 A SE8007396 A SE 8007396A SE 8007396 A SE8007396 A SE 8007396A SE 8007396 L SE8007396 L SE 8007396L
Authority
SE
Sweden
Prior art keywords
conjugate
specific binding
liquid medium
ligand
bound
Prior art date
Application number
SE8007396A
Other languages
Swedish (sv)
Other versions
SE447510B (en
Inventor
R C Boguslaski
R J Carrico
J E Christner
Original Assignee
Miles Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Lab filed Critical Miles Lab
Publication of SE8007396L publication Critical patent/SE8007396L/en
Publication of SE447510B publication Critical patent/SE447510B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/44—Iso-indoles; Hydrogenated iso-indoles
    • C07D209/48—Iso-indoles; Hydrogenated iso-indoles with oxygen atoms in positions 1 and 3, e.g. phthalimide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D237/00—Heterocyclic compounds containing 1,2-diazine or hydrogenated 1,2-diazine rings
    • C07D237/26—Heterocyclic compounds containing 1,2-diazine or hydrogenated 1,2-diazine rings condensed with carbocyclic rings or ring systems
    • C07D237/30—Phthalazines
    • C07D237/32—Phthalazines with oxygen atoms directly attached to carbon atoms of the nitrogen-containing ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
    • C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
    • C07D493/10—Spiro-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D495/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms
    • C07D495/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having sulfur atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
    • C07D495/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

A test process operating using a specific bond for the investigation of a liquid medium on a ligand is described. The test process comprises the following steps: (a) the liquid medium is brought into contact with a reagent which contains a conjugate of a labelling substance which has a predetermined characteristic and a specific binding substance, the reagent and the ligand forming a binding reaction system which (i) forms a bound phase of the labelling conjugate, in which the specific binding fraction is bound thereto by a specific binding component, and (ii) forms a free phase of the labelling conjugate, in which the specific binding fraction is not bound thereto by a specific binding component, (b) determination of the said characteristics either in the bound phase or in the free phase as a measure of the said ligands in the said liquid medium. The said characteristic of the labelling substance proves to be active in the following reaction systems: (1) as a substrate in an enzyme-catalysed reaction, (2) as a reactant in a cyclic reaction system, (3) as a reactant in a chemiluminescent reaction system, or (4) as a coenzyme in an enzyme-catalysed reaction.

Claims (1)

1. Testforfarande utnyttjande en specifik bindningsreaktion fir att i ett vatskemedium bestamma en ligand maid bland antigener och antikroppar dErtill; haptener och antikroppar dartill; hormoner, vitaminer, metaboliter och farmakologiska Tiedel saint deras receptorer Oct bindningssubstanser; mid vilket farfarande man (1) bringar vatskemediet i kontakt med ett reagens innefattande ett markt konjugat till bildning av en bunden form av del markta konjugatet och en fri form av dot markta konjugatet, varvid proportionerna mellan den bundna formen och den fria formen av Get markta konjugatet ar en funktion am koncentrationen av ligand i vatskemediet, vare'fter man (2) mdter mangden markningssubstans i den bundna formen eller den fra formen av dot mArkta konjugatet,kanneteeknat av att ett enzymsubstra anvandes sdsom markningssubstans.A test method utilizing a specific binding reaction to determine in a liquid medium a ligand maid among antigens and antibodies thereto; haptens and antibodies thereto; hormones, vitamins, metabolites and pharmacological Tiedel saint their receptors Oct binding substances; by which method the liquid medium is contacted with a reagent comprising a marked conjugate to form a bound form of the del markta conjugate and a free form of the dot markta conjugate, the proportions between the bound form and the free form of Get markta The conjugate is a function of the concentration of ligand in the liquid medium, after which (2) the amount of label in the bound form or that from the form of the dot labeled conjugate is measured, characterized in that an enzyme substrate is used as the label.
SE8007396A 1975-04-28 1980-10-21 METHOD AND REAGENT OF DETERMINATION OF A LIGAND IN A SCIENTIFIC MEDIUM SE447510B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US57200875A 1975-04-28 1975-04-28

Publications (2)

Publication Number Publication Date
SE8007396L true SE8007396L (en) 1980-10-21
SE447510B SE447510B (en) 1986-11-17

Family

ID=24285954

Family Applications (3)

Application Number Title Priority Date Filing Date
SE7604841A SE440280B (en) 1975-04-28 1976-04-27 TEST PROCEDURE AND REAGENTS WITH COENSE SYMBOLS
SE8007397A SE451895B (en) 1975-04-28 1980-10-21 Chemiluminescence Procedure for Determination of a Ligand in a Liquid Medium
SE8007396A SE447510B (en) 1975-04-28 1980-10-21 METHOD AND REAGENT OF DETERMINATION OF A LIGAND IN A SCIENTIFIC MEDIUM

Family Applications Before (2)

Application Number Title Priority Date Filing Date
SE7604841A SE440280B (en) 1975-04-28 1976-04-27 TEST PROCEDURE AND REAGENTS WITH COENSE SYMBOLS
SE8007397A SE451895B (en) 1975-04-28 1980-10-21 Chemiluminescence Procedure for Determination of a Ligand in a Liquid Medium

Country Status (23)

Country Link
JP (6) JPS604939B2 (en)
AT (1) AT357685B (en)
AU (2) AU509182B2 (en)
BE (1) BE841179A (en)
BR (1) BR7602561A (en)
CA (2) CA1078712A (en)
CH (1) CH635438A5 (en)
DD (1) DD125231A5 (en)
DE (3) DE2660548C2 (en)
DK (3) DK149969C (en)
ES (1) ES447378A1 (en)
FI (1) FI68324C (en)
FR (1) FR2332533A1 (en)
GB (2) GB1552607A (en)
HU (1) HU179542B (en)
IE (1) IE42544B1 (en)
IL (1) IL49354A (en)
IN (1) IN142734B (en)
IT (1) IT1064132B (en)
LU (1) LU74834A1 (en)
NL (1) NL181281C (en)
SE (3) SE440280B (en)
ZA (1) ZA762030B (en)

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US5605800A (en) * 1978-04-13 1997-02-25 Institut Pasteur Method of detecting and characterizing a nucleic acid or a sequence of the latter, and enzymatic reactant for the application of this method
IL57570A (en) * 1978-06-22 1982-07-30 Miles Lab Method and reagent for specific binding assay with a prosthetic group as a label component
DE2924332A1 (en) * 1978-06-22 1980-01-03 Miles Lab FLAVIN-ADENINE-DINUCLEOTIDE-MARKED CONJUGATE AND ITS USE IN SPECIFIC BINDING TRIALS
US4225485A (en) * 1978-07-24 1980-09-30 Miles Laboratories, Inc. Chemiluminescent naphthalene-1,2-dicarboxylic acid hydrazide-labeled polypeptides and proteins
US4334069A (en) * 1978-07-24 1982-06-08 Miles Laboratories, Inc. Chemiluminescent phthalhydrazide-labeled hapten conjugates
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DK256581A (en) * 1980-06-13 1981-12-14 Nat Res Dev BINDING ASSAY
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CA1183450A (en) * 1980-10-30 1985-03-05 Alfred C. Greenquist Homogeneous specific binding assay device and method
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US4366243A (en) 1981-04-17 1982-12-28 Miles Laboratories, Inc. Stabilization of glucose oxidase apoenzyme
JPS5818167A (en) * 1981-07-24 1983-02-02 Fuji Photo Film Co Ltd Analyzing film and analysis using said film
JPS5877843U (en) * 1981-11-20 1983-05-26 株式会社三協精機製作所 tape recorder
GB2112779B (en) * 1981-12-11 1986-10-15 Welsh Nat School Med Aryl acridinium esters as luminescent labelling materials
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FR2519004B1 (en) * 1981-12-29 1985-09-27 Pasteur Institut MODIFIED ADENOSINE 5'-TRIPHOSPHORIC ACID AND METHOD FOR DETERMINING BIOLOGICAL SUBSTANCES LIKELY TO FIX ADENOSINE 5'-TRIPHOSPHORIC ACID DEGRADATION PRODUCTS
US4709016A (en) * 1982-02-01 1987-11-24 Northeastern University Molecular analytical release tags and their use in chemical analysis
US5650270A (en) * 1982-02-01 1997-07-22 Northeastern University Molecular analytical release tags and their use in chemical analysis
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Also Published As

Publication number Publication date
ATA309376A (en) 1979-12-15
FI761148A (en) 1976-10-29
DD125231A5 (en) 1977-04-06
SE451895B (en) 1987-11-02
DK150808C (en) 1988-02-15
SE8007397L (en) 1980-10-21
CH635438A5 (en) 1983-03-31
SE440280B (en) 1985-07-22
JPH0137694B2 (en) 1989-08-09
BR7602561A (en) 1976-11-23
SE7604841L (en) 1976-10-29
JPS5951353A (en) 1984-03-24
IT1064132B (en) 1985-02-18
NL181281C (en) 1987-07-16
FR2332533B1 (en) 1981-12-18
JPH0145026B2 (en) 1989-10-02
DK158366C (en) 1990-10-01
IN142734B (en) 1977-08-20
HU179542B (en) 1982-11-29
ES447378A1 (en) 1977-11-01
FR2332533A1 (en) 1977-06-17
DE2618419C2 (en) 1983-02-10
AU509182B2 (en) 1980-04-24
JPH0145027B2 (en) 1989-10-02
JPH0137693B2 (en) 1989-08-09
IE42544B1 (en) 1980-08-27
JPS51146295A (en) 1976-12-15
FI68324B (en) 1985-04-30
ZA762030B (en) 1977-08-31
IL49354A (en) 1979-03-12
AU1335376A (en) 1977-11-03
IL49354A0 (en) 1976-06-30
DK576281A (en) 1981-12-23
JPS5951354A (en) 1984-03-24
SE447510B (en) 1986-11-17
DE2660548C2 (en) 1984-04-12
JPS6052378B2 (en) 1985-11-19
DK158366B (en) 1990-05-07
CA1082577A (en) 1980-07-29
JPS5942453A (en) 1984-03-09
FI68324C (en) 1985-08-12
DE2618511B2 (en) 1980-08-14
BE841179A (en) 1976-08-16
DK149969C (en) 1988-08-01
JPS5267388A (en) 1977-06-03
JPS5942454A (en) 1984-03-09
DE2618511C3 (en) 1981-05-07
GB1548741A (en) 1979-07-18
NL7604420A (en) 1976-11-01
DK150808B (en) 1987-06-22
CA1078712A (en) 1980-06-03
AT357685B (en) 1980-07-25
LU74834A1 (en) 1977-01-12
AU1335276A (en) 1977-11-03
GB1552607A (en) 1979-09-19
JPS604939B2 (en) 1985-02-07
DE2618419A1 (en) 1976-11-04
DK189176A (en) 1976-10-29
AU502726B2 (en) 1979-08-09
DK576181A (en) 1981-12-23
IE42544L (en) 1976-10-28
DE2618511A1 (en) 1976-11-04
DK149969B (en) 1986-11-03
NL181281B (en) 1987-02-16

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