RU2736356C2 - Method for initiation of tumor cell death with ascorbic acid and hf and uhf wave radiation energy - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to a method for initiation of tumor cell death, intended for complex treatment of oncological patients having tumor tissues in all organs of human body, by their hyperthermia HF and microwave energy. Method consists in the fact that a person is transferred to a non-carbohydrate diet for 3 days to create glucose starvation and subsequent maximal saturation of oncological cells with electron-ionic solution of ascorbic acid in disposable megadose 350 mg, and after 4 hours with maximum accumulation of preparation in tumor tissues is 2–4 times higher than in healthy, selective hyperthermia of tumor tissues is performed with high-frequency energy in accordance with the depth of their location and the penetration depth of the electromagnetic wave into the human body of 1100 cm, at the allowed frequency f=13.56 MHz, with total heating rate of tumor tissues at these frequencies of 0.017 °C/s, for 360 s to the temperature of tumor tissues 43 °C, when heating healthy tissues no more than 40 °C.

EFFECT: said method results in thermal death of tumor tissues due to high dielectric contrast of the tumor and increasing difference in its conductivity (dielectric loss) relative to surrounding healthy biological tissues, which reaches multiple values for various tumors.

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Description

The invention relates to medicine and is intended to induce the death of tumor cells in living biological objects with ascorbic acid and the energy of wave HF and UHF radiation, known as HF and UHF hyperthermia.

In medicine, hyperthermia is referred to as a significant increase in a person's body temperature over 40 ° C. Hyperthermia for cancer treatment has been used as early as half a century ago. The German physician von Ardenne opened a "thermal" clinic in a water bath for hopelessly cancer patients, whom he heated to 42 ° C. After such a procedure, no more than 17% of people survived, but they were completely cured. The rest died, unable to withstand such high temperatures. This technology is still used in the USA, where the human body is heated to 42.5 ° C, with its subsequent return to life. This treatment technology can be effectively used for selective heating of oncological tissues with HF and microwave energy without a significant increase in the temperature of healthy tissues surrounding the tumor.

The method of initiating the death of tumor cells by the electromagnetic energy of the HF and UHF wave radiation consists in the complex simultaneous action of ascorbic acid (AA) and wave irradiation with HF and UHF electromagnetic energy. After taking a solution of ascorbic acid for its preservation in a significant amount in the tumor tissues of the human body, it takes time for its accumulation in the tumor tissues for 2-4 hours. The maximum content of ascorbic acid in tumor tissues occurs precisely during this period of time, and it is several times higher in tumor tissues than in healthy ones due to selective absorption of acid by tumor tissues and healthy tissues (AA); it is excreted in the urine within 30 minutes. Some researchers point to the fact that AA accumulation in tumor tissues is 50-100 times higher than in human blood. AA is completely absorbed by the intestines, 30-60 minutes after ingestion of 200-300 mg of AA, and its transition to dehydro-ascorbic acid (D-AA), its ionized and fat-soluble form, which has permeability through cell membranes, with a positive charge 40 times higher than AA during its transportation into oncocells, through a ritrocyte negatively charged by riticulum, with maximum saturation of metochondria, and its half-life within 2-6 minutes and conversion into AA and its complete removal from oncocells 4-7 hours after administration.

The renowned scientist Launus Pauling conducted scientific data on the positive effect of ascorbic acid (AA) on slowing down the cancer process. Some experts explained this by the fact that AK was taken by sick people in large doses in the form of tablets and did not always give a positive effect due to the fact that the concentration of AK in the tumor itself was low. It has been suggested that the tumor suppression mechanism is associated not with AA, but with its oxidized form. This has been confirmed by scientific research. In experiments using Dehydro-AK, the results were continually renewed. These results were published in the 1980s and early 1990s. Their most complete description was given in 1991 in the journal of the American Association for Clinical Nutrition. Good results were obtained for leukemia in mice. The skepticism of the scientific world, however, did not allow us to pay serious attention to these data. Lonely physicians continued research on the treatment of cancer with vitamin C, without delving into especially the theoretical basis of this process. A Canadian researcher John Teway tried to uncover the mechanisms of action of AK on tumor cells. His final work was published in Cancerletters in 2008. Currently, physicians use AK as an additional agent that reduces the side effects of chemotherapy.

Tumor cells accumulate, in contrast to normal cells, a significant amount of homocysteine teolactone (HTL). Before homocysteine is inserted into a protein, it becomes a biological marriage, in the form (HTL). In ordinary cells, there is little homocysteine, therefore, theolactone is practically not formed from it, but transformation into a cancer cell requires significant activation of methylation, which in turn starts a special biochemical cycle in which homocysteine is involved. In this case, the protein that synthesizes the machine of the cancer cell works at full capacity, so it is more often mistaken. Teway discovered that interaction with dehydroascorbic acid, this substance forms the highly toxic 3-mercaptopropionic aldehyde (MPA). When dehydroascorbic acid enters a cancer cell saturated with HTL, MPA is formed, which kills the cancer cells. Destroying cancer cells, MPA eliminates the source of its formation, so normal cells do not suffer from it much. In this case, it can be argued that in the treatment of cancer with Dehydroascorbic acid, obtained as a result of AA oxidation, both in the blood and in the human digestive system, therapeutic effect.

A number of researchers argue that the minimum concentration of vitamin C, which is a prooxidant (lyco-oxidizing compounds that neutralize free radicals), in the blood and tissues kill cancer cells without affecting healthy ones, due to the damage caused by local oxidative stress, the process of damage as a result of oxidation, of cellular DNA and depletion of adenosine triphosphate (ATP) - the energy source of the cell. Hydrogen peroxide, among other accompanying molecules, of aggressive action, causes a malfunction of the functioning of a certain enzyme responsible for "feeding" the cells of malignant tumors. AA is a representative of intermediate acids that can accumulate in the cytosol of cells.

The proposed complex of dehydroascorbic acid, obtained as a result of the oxidation of AA of the drug "Ascorbic acid", is oxidized by hydrogen peroxide of a derivative of dehydroascorbic acid (AA) in an alkaline medium, the reaction is catalyzed by iron heme, and causes chemilumination under the action of photowave high frequency and microwave hyperthermia, with active release of singlet oxygen ... If an oxidizing agent - hydrogen peroxide is added to an alkaline solution of cancer cells, then a glow occurs. In the presence of catalysts, this glow intensifies and becomes brighter. The role of the catalysts for the solution of Dehydroascorbic and Dehydrofolic acids obtained as a result of the oxidation of the AA of the preparation "Ascorbic acid" is performed by blood iron hemin and various sodium compounds. These chemical activators of chemiluminescence enter into chemical reactions with reactive oxygen species or organic free radicals, during which cell molecules are formed in an excited electronic state. The observation with this glow is associated with the transition of molecules to their ground state, which leads to the emission of photons. The activator of the excited state is Dehydroascorbic and

Dehydrofolic acids obtained as a result of the oxidation of AA of the "AK" preparation in the presence of oxygen radicals. Under the action of the AA oxidant - hydrogen peroxide radicals, the formation occurs, which reacts with the superoxide radical, which forms the internal peroxide (dioxide), which leads to the formation of excited molecules of the drug "Askofol". The transition of these molecules to the ground initial state is accompanied by emission of a quantum of light. Hydrogen peroxide AK is the main participant in the formation of free radicals, it is constantly formed in small quantities in the human body, it is a relatively harmless compound, but in the presence of metal ions of variable valence iron, copper, manganese and chromium or hemin compounds from hydrogen peroxide Н ₂ О ₂ , a destructive hydroxyl JOH radical, causing mutations and inactivation of enzymes and damage to biological membranes of cancer cells. The hydroxyl group of enzymes activates molecules, and actively enters into a chemical reaction with it, which leads to a bright glow of tumor tissues.

When placed in a variable electromagnetic field of high intensity and frequency of various biological bodies, they also begin to emit a characteristic radiance of varying intensity and colors, by which one can judge the properties of the object under study. The method of "high-frequency photography" (the Kirlian effect, Kirlianography in honor of the inventor V.Kh. Kirlian) is now widely known in Russia and abroad as a method of experimental research of electromagnetic fields and bioenergetic interactions. But the research of the glow of biological objects in a variable electromagnetic field of high frequency is of the greatest scientific and practical interest. explained

photoelectromagnetic effect of photowave radiation and luminescence of biological objects.

In accordance with modern concepts aqueous solutions of alkalis and acids in a human body is considered as the associated liquid consisting of separate associated elements - neutral clusters and cluster ions of the general formula (H ₂ O) _n, [(H ₂ O) _n] ^+, [( H ₂ O) _n ] ^- , [(NO ₂ ) _n ], [(H ₂ O ₂ ) _n ], [(NaO ₂ ) _n ] [(ClO ₂ ) _n ], [(CO ₂ ) _n ], etc. .d, where the number of hydrogen-bonded water molecules can reach, according to some authors, hundreds and even thousands of units under the action of HF and UHF energy by n times. These effects respectively alter the electrical conductivity and biophotoluminescence of biological tissues. A change in the position of one structural element (water molecule) under the influence of any external factor or a change in the orientation of the surrounding neighboring water molecules in the cells provides high sensitivity of the entire information system of water to various external influences (electromagnetic, thermal, sound fields, bioinfluence, etc.). In addition, in water clusters, due to the interaction between covalent and hydrogen bonds between oxygen atoms and hydrogen atoms, the proton (H ⁺ ) can migrate according to the relay mechanism, leading to proton delocalization within the cluster, providing the release of singlet oxygen with a characteristic bright glow that kills cancer cells. This property explains the extremely labile, mobile nature of the interaction of clusters with each other.

The structured state of aqueous solutions is a sensitive sensor of various fields - electromagnetic, acoustic, energy-information, etc. In addition, aqueous solutions of various chemical elements are a source of superweak and weak alternating electromagnetic radiation. In this case, induction of an external electromagnetic field can occur, causing resonant effects of the combination (superposition) of external electromagnetic fields with their own fields in biological objects with photowave radiation, capable of changing the structural and informational characteristics of biological objects, 80-90% consisting of water solutions with various chemical impurities and cause their photoluminescence.

Under the influence of a high-frequency electromagnetic field in biological objects and aqueous solutions of various chemicals, excitation, polarization and ionization of N ₂ , H ₂ , O ₂ and CO ₂ molecules occur. As a result, an ionized gas is formed with separated electrons having negative charges, which create an electrically conductive medium for the formation of a corona discharge in biological objects of various colors, which, depending on the electrically conductive properties of an object saturated with various chemical solutions, can color the glow crown in different colors. The shape of the glow crown, its density, brightness and surface distribution are determined mainly by the electromagnetic parameters of the object.

Some cells of the body, granulocytes and monocytes in the blood, and tissue macrophages, in the fight against foreign cells, release reactive forms of singlet oxygen contained in superoxide radicals, hydrogen peroxide H ₂ O ₂ , and the hydroxyl radical JOH in this case, weak chemilumination is observed, which increases many times in the presence of D-AK and AK with HF and microwave irradiation. These effects are also greatly enhanced by the action of short-term electrical impulses on blood vessels and cells, causing an increase in the permeability of cell membranes - riticulomas and stimulation of the release of reactive oxygen species by the metachondria. This effect of electrical impulses at the beginning of the 19th century was successfully demonstrated to the public by Nikola Tesla, when exposed to pulsed high-frequency energy of vessels with liquids capable of emitting light and luminescent lamps, which, without being connected to electrical wires, shone with bright light in the hands of Nikola Tesla, with which he also juggled, which caused genuine delight among the audience, while at that time inexplicable by nature, a phenomenon that only Nikolo Teslo knew.

In biology, these factors are called collective luminescence stimuli that change the state of blood and tissue phagocytes and their ability to increase the release of reactive oxygen species, and, accordingly, the protective functions of cells.

In cancer cells, aerobic respiration is absent in mitachondria and is replaced by glycolysis. When AA enters the oncocell, it inhibits glycolysis and transfers it to the path of normal aerobic activity. Perhaps this is due to the competitive presence of glucose. To completely disable glycolysis in tumor cells, it is necessary to completely exclude the access of glucose, or so that UC prevails over glucose in the substrate. In healthy cells, in any amount in cytazole, it exhibits protective antioxidant properties. In cancer cells, with an excess of it, it stimulates the oxidation processes, due to their enrichment with bivalent oxygen, which, when it is overabundant, has a toxic effect on the cancer cells.

In this case, it can be argued that there would be even fewer side effects, and the effect is higher if treatment with powerful doses of AA was based on the background of complete overlap of the intake of carbohydrates - glucose, as AA competitors in cancer cells. To do this, in our opinion, it is necessary to transfer a person to a carbohydrate-free diet for 3 days, for a complete absence at this time in the human diet of carbohydrates, which in the gastrointestinal tract are converted into glucose, which is extremely necessary for the nutrition of cancer cells. With such an introduction of onco cells into artificial glucose "starvation", then a person needs to inject intravenously high doses of vitamin C, better than its oxidized form and Dehydro-AA. When it enters the blood vessels of the tumor, which has a large branched network with thin peripheral vessels and a low speed of movement in the blood in them. The blood flow in these vessels of tumor tissues decreases even more when they are heated, which, even more efficiently, leads to blood coagulation in the vessels of tumor tissues, not allowing them to cool, due to the absence of a closed circulatory system. In the main human organs, rich in blood vessels, closed in the main circulatory system, there is a cooling of the borderline healthy tissues exposed to HF and UHF electromagnetic hyperthermia. A "hungry" tumor is saturated with Dehydro-AA within 0.5-4 hours, several times higher than in normal healthy tissues, stimulates the formation of hydrogen peroxide in a sufficiently large amount in the interstitial fluid. It is this chemical compound that is formed during the interaction of vitamin C and the internal environment of the body. Peroxide is a factor or hormone that stimulates the mechanisms of self-destruction and death of cancer cells. The formation of sufficient doses of hydrogen peroxide around and inside the tumor cells and their apoptosis is possible only with a sufficiently large amount of intake of ascorbic acid. Under these conditions, D-AA can act as an antioxidant or prooxidant, i.e. an oxidizing agent, including showing a destructive rather than a creative property of cancer cells. This is very important in the energy of cells. Therefore, AK can be designated as a switch of metabolism, which accelerates and optimizes aerobic energy metabolism in normal cells, stimulates tissue respiration and the formation of ATP. In cancer cells, aerobic respiration is absent in mitachondria and is replaced by glycolysis. Upon entering the oncocell, AA inhibits glycolysis, but is unable to transfer it to the path of normal aerobic activity. Perhaps this is due to the competitive presence of glucose. To completely disable glycolysis in tumor cells, it is necessary to completely exclude the access of glucose or for AA to prevail over glucose in the substrate. In healthy cells in small amounts in cytazole, it exhibits protective antioxidant properties. In cancer cells, with an excess of it, it stimulates the oxidation processes, with the formation of hydrogen peroxide and lipid peroxides, which, when they are in excess, have a toxic effect on onco cells.

Dehydroascorbic acid is actively imported into the endoplasmic reticulum (EPR) (Endoplasmic reticulum, consisting of membranes and directing, and active transport of substrates against gradients) of cells with the help of glucose transporters. It should be noted that energy processes in cancer cells are transferred from the metachondria to the endoplasmic reticulum. It is here in EGGR that D-AA accumulates and the environment of the oncocell in this place differs significantly from ordinary cells, it is simply re-reduced here, and here D-AA is obviously forced to recover to AA. From this moment, the destructive effect of AK on the oncocell begins. A "hungry" cancer cell at this time can repeatedly accumulate D-AK in itself, because perceives it on its membrane transporters for glucose. Since there are many times more glucose-consuming receptors in the oncolet than in healthy people, although the transport systems for the delivery of glucose and D-AA into the cell are common, this is the "Trojan horse" for oncocells. Thus, one can very simply deceive the oncocells and inject D-AK into them, with the solution of the problem of supplying megadoses of D-AK, and then the phenomenon of death of onco cells will be greatly enhanced.

A "hungry" tumor is maximally saturated with D-AA, 2-6 times higher than in normal healthy tissues, in a sufficiently large amount on membranes and interstitial fluid. A "hungry" tumor in the absence of glycolysis saturates within 2-4 hours, and is many times higher than in normal healthy tissues, stimulates the formation of macrophages and T-lymphocytes under the action of the enzyme ferrachelatase, in a sufficiently large amount on membranes and interstitial fluid. It is this chemical compound that is formed during the interaction of AA and D-AA and hydrogen peroxide in the internal environment of the body. Under the action of the oxidizing agent of lipoperoxide radicals and the formation of hydrogen, significantly enhanced by the temperature and action and additional photodynamic action of the HF and microwave electromagnetic fields, active hydrogen and oxygen radicals are formed, which then reacts with superoxide radicals, accelerating and forming internal peroxide (dioxide), H ₂ O ₂ during their hyperthermal decomposition, high-frequency and microwave energy of the AA of vitamin C. In this case, there is a multiple increase in the formation of excited oxygen molecules. The transition of AA molecules and internal ascorbic acid peroxide D-AA from the excited state to the ground state is accompanied by the emission of light quanta and a strong glow. As a result of these chemical reactions associated with a high release of reactive forms of hydrogen and oxygen and organic free radicals, significantly enhanced temperature action and additional photodynamic action of electromagnetic fields of HF and microwave photowave radiation, oncological cells are burned out.

The method of "selective starvation" of superficial and deeply located onco cells in the human body, by subsequent administration or intake of various sensitizers, for selective maximum saturation of tumor cells with highly electrically conductive electron-ion solutions of electrophotosensitizers with a maximum separation of the electrophysical properties of tumor and healthy tissues with subsequent selective action on them electromagnetic fields of high frequency in combination with other methods - this is the most relevant scientific and practical direction in the fight against oncological diseases.

The study of biophysical and biochemical mechanisms determine two concepts of cancer cell death, one assumes the significance of D-AA, and the other AA, which in both cases leads to the apparent death of onco cells. The main task for researchers remains to enhance as much as possible the effect of selective absorption of D-AK and AK by cancer cells, with simultaneous subsequent high-frequency irradiation of cancer cells, in order to increase the effectiveness of treatment up to 100%.

It has already been proven that such an effect is possible by the example of disinfection of biological objects from viral, fungal and bacterial infections with high-frequency and microwave energy. Numerous studies conducted by us in the Krasnoyarsk State Agrarian University and the VIZR in St. Petersburg have confirmed 100% efficiency of disinfection of vegetable seeds and living biological objects saturated highly conductive electronic-ionic solutions of microelements of HF and UHF energy against viral infections, which have a similar origin with cancer cells.

A.S. No. 563938 USSR. Method of processing seeds of agricultural crops / Tsuglenok N.V., Tsuglenok G.I. - Publ. 03/16/1977, Bul. No. 25. Certificate of the USSR No. 950214. Method of pre-sowing seed treatment / Tsuglenok N.V. - Registered in the registry on 04/14/1982. 45. Intensification of thermal processes of seed preparation for sowing with HF and SHF energy: guidelines / N.V. Zuglenok. - M .: Agropromizdat, 1989. Methodical recommendations for the use of high-frequency and microwave energy in the preparation of seeds for sowing / N.V. Zuglenok. - M .: RZh Gosagroprom of the USSR, 1989 .-- 19 p. Ways of disinfection of tomato seeds against viral infection / Yu.I. Vlasov [et al.] // All-Russian Research Institute of Plant Protection (VIZR). - 1989. - T. 71. - S. 49-54. Method of disinfection of egg powder. Patent number: 1734632. Published: 23.05.1992 Authors: Tsuglenok N.V., Kolmakov Yu.V. IPC: A23v 5/02. Method of preparing a medium for diluting semen from the manufacturer. Patent number: 1769422. Published: 27.06.1995. Authors: Tsuglenok, Ostashko, Shakhmatov, Silantyeva, Kontsedal.

It has been proven that under the influence of carcinogens, oncoviruses are incorporated into a healthy cell and eventually dissolve in it, turning it into an oncocell. Any viruses are killed by temperature or acid. Other methods against oncoviruses and cancer cells are basically powerless, they simply are not. A new photodynamic method of using laser photosensitizers deserves special attention in this direction. But the shallow depth of penetration of the electromagnetic wave of laser emitters does not allow burning deep-seated malignant tumors.

It is necessary to note another very important biophysical process - an increase in the electrical conductivity of viruses consisting of a protein envelope filled with a mixture of nucleic acids and similarly tumor tissues filled with solutions of intercellular fluid determined by a significant concentration of ions and electrons and their mobility in comparison with healthy tissues. HF and microwave heating in tumor tissues significantly increases the mobility of ions and electrons, increasing their electrical conductivity and dielectric losses, which further enhances their selective heating and opoptosis of tumor tissues.

It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects.

This curing effect is explained by the fact that at this time from 0.5 to 1 hour in normal cells of living biological objects, ascorbic acid is rapidly converted into dehydroascorbic acid and bivalent iron heme, under the action of the ferrochelatase enzyme, and accumulates as much as possible in cancer cells within 2-4 hours. exceeding 50-100 times its content in human blood. At the same time, a high contrast of the content of dehydroascorbic acid and its transformation into ascorbic acid in the tumor remains, which significantly increases its electrical conductivity with a significant change in the dielectric properties of tumors relative to the surrounding healthy biological tissues, reaching this difference several times.

With further hyperthermia of tumor cells saturated with ascorbic acid, for 360 seconds by wave radiation of HF and microwave fields, with an allowed frequency of oscillations of the electromagnetic field f = 433 92 MHz, f = 915 MHz or 2450 MHz, with a heating rate of 0.017 ° C / sec to the final heating temperature of tumor cells is 43 ° C. This allows the simultaneous conduct of fluorescent diagnostics to clarify the border of tumors and simultaneous hyperthermia of tumor cells with the energy of photowave radiation and to identify and destroy, thus, even undetectable tumor formations located in the deep layers of a biological object.

The biophysical meaning of this method lies in the selective maximum saturation and accumulation in tumor cells of highly electrically conductive electron-ionic solutions of electrophotosensitizers in tumor cells in this case with ascorbic acid and in determining the difference in electrical potentials in the intercellular medium and on the walls of the reticulum. The reticulum is an electrical circuit, where negative charges are evidently accumulated on one side of the membrane, and positive ones on the opposite side, therefore the reticulum is an electric transporter of glucose and other nutrients of cancer and healthy cells. Consequently, the reticulum is an electrical network charged with negative and positive charges. The balance of these charges is strictly controlled by the activity of the metachondria and the energetic operator structures on the outer side of the cell - on the cilia. These proteins under certain situations in the environment of the cell, being discharged, can give an active signal to the reticulum and metachondria. This changes the balance that exists on one side of the reticulum. This leads to a shift in chemical processes, many new reactions are triggered. One side of the reticulum membrane is connected to one type of entrance to the metachondria, and the opposite side to the exit from it. Thus, a single electrical circuit is created for double active control of the energy of the metachondria. The strength of the electric field on the reticulum keeps the metachondria under control. In this case, the metachondria draw in the charges accumulated on one side of the reticulum membrane and remove opposite charges to the other side of the reticulum membrane. The charges are thus not mixed and separated. This is important for ion exchange to take place in cells. Externally, the reticulum is similar to the capacitor plates, the more layers of plates, the greater its electrical capacity. Between the spacers there is a semiconductor saturated with dehydroascorbic and ascorbic acids. This capacitor, i.e. a powerful dense network of tumor sheaths-membranes is very clearly visible through a microscope. The number of membranes in tumor cells is much higher than in healthy ones. Accordingly, the density of tumor tissues and the capacity of the biological electrical capacitor are significantly higher than those of healthy tissues. When charging, negatively charged particles-electrons will be collected on one plate of such a capacitor, and ions, positively charged particles, will be collected on the other. Such a charged capacitor can turn into a current source if disconnected. Any fluctuations in the external field on the outer side of the cell membrane affects the state of the reticulum, which is dumped on the metachondria, controlling their activity. The metachondria, in turn, are tuned so that they never allow the charges on the reticulums to drop below a critical level. In cancer cells, the charges inside the metachodria are sharply reduced and the entire regulation system is disrupted. This is the main control rod for the entire electrochemical energy of the cell. Therefore, chemical processes are always secondary and not primary. As a result of the electrochemical energetics of the cell in the reticulum there is a circulation of substances, where the metachondria are the pump. With a lack of this cycle between the reticules and metachondria, due to electroosmosis, substances are sucked from the outside through the outer membrane and the gateways and sodium pump are opened on it. The medium on the membranes of the reticulum and the liquid substrate is reconstituted due to the excess of negative charges. This is what determines the chemical equilibrium in terms of pH, conjugated buffer chemical electropheral substances when the buffer system is discharged or restored. These processes are regulated by charges on the lining of the reticulum and metachondria. Chemical processes, in this case, are simply executors, intermediaries. The outer side of the metachondria provides a voltage dependent anion channel. This voltage maintenance mechanism is called VDAC, which sets the conditions for the work of the reticulum. It is here on the outer side of the membrane that the Hexokinase II enzyme is located, which ensures the utilization of glucose. Separation, disconnection of the work of the outer mitochondrial membrane (VDAC) and Hexokinase II ensures the induction of apoptosis.

The metachondria works by pulling from the reticulum into itself as an electromagnetic pump, which requires power under high voltage. Without this effect, the highest tension of pulling in nutrients into the cell will not be possible. This process of self-regulation of metabolism includes the so-called cilia and conformational proteins, which work as a single closed energy circuit. Cancer cells, unlike normal cells, do not have cilia. This most damaging energy level is absent in cancer cells. The only correct way to find a weak spot in the energy of cancer cells and thereby destroy them. Metachondria set the degree of charge to ion pumps on the outer membrane of the cell and to starter structures that hold charges on the reticulum. These sensory structures can be most rapidly damaged and burned out, since metachodria are the most efficient electrochemical furnaces. In the case of turning off the metachondria, the voltage gradient of the cell decreases sharply and the processes take place in onco cells over a much larger area, which allows them to burn a lot of glucose and other substrates such as ketones. There is no high degree of glucose combustion here. The oncocell takes not quality, since everything is concentrated on a small area of metachondria, but with a larger number, much more than in healthy cells and, accordingly, at high potentials on the capacitor plates, i.e. a large amount of oxidation-combustion area on the walls of the reticulum network. Therefore, such a cell does not need oxygen, but the consumption of glucose will be much greater than in healthy tissues.

The membranes of the reticulum and the nucleus of the cell are the same, and the reticulum, as a capacitor, is contoured to the nucleus by only one side of it - an electron and dumps electrons into the nucleus. Thus, the charge in the reticulum also provides charge inside the cell nucleus. The cell nucleus is saturated with electrophilic proteins, which provide the concentration of a super-powerful electrostatic charge inside the nucleus.

In healthy cells, D-AA entering the EPR will not be restored to AA, because pH and ORP (oxidation-reduction potential) are not suitable for this, and D-AK will be practically harmless for them and will be transformed on a glucose conveyor. In oncocells, the environment is different, reconstituted into ascorbic acid, which tries to burn and destroy everything in the oncocell to the maximum, due to lipid peroxidation (LPO). In this case, significant destruction occurs with the formation of toxic lipoperoxides, damage to cell membranes, various organelles, mutation of nucleic acids, inoculation of enzymes, destruction of nutrients and cell death. In this case, cell death proceeds not along the path of apoptosis, but outright necrosis.

Ascorbic acid in cancer cells is converted to Dehydroascorbic acid and hydrogen peroxide. The more dehydroascorbic acid in the oncocell, the more hydrogen peroxide is formed there, in comparison with healthy cells. Excess peroxide triggers the death of cancer cells. The process of cancer cell death is initiated by HF and SHF field by rapidly heating the cancer cells to 43 ° C, due to AA and D-AA and their rapid oxidative decomposition under the influence of temperature with a large release of hydrogen peroxide, which is fatal for cancer cells.

The most complete accumulation of ascorbic acid in the tumor occurs within 4 hours after ingestion. Then the level of ascorbic acid in the tumor gradually decreases, reaching the initial values 4-7 hours after taking the drug. The drug Sodium Ascorbinate is taken in megadoses in the form of a 5-10% solution orally up to 500 mg in the form of a solution in 50-200 ml of drinking water or in ampoules of 1 and 5 ml for injection into a vein 4 hours before the diagnosis and HF and microwave electromagnetic hyperthermia of tumor tissues with the energy of wave radiation, after which at a heating rate of 0.0171 ° C / s for 360 seconds, to a temperature of 43 ° C, as a result of which the tumors are denatured and, as a result, the decay products of tumor cells are excreted by the body independently within 2 4 weeks, in a natural way, excluding surgical intervention in the human body.

The main task for researchers remains to maximize the effect of maximum selective absorption of ascorbic acid by cancer cells and to increase the effectiveness of treatment by increasing the electrical conductivity of metachondria and reticulums of cancer cells. It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects. The electrical conductivity of cancer cells is due to the presence of mobile charged electrons in the reticulums and in the cell nucleus and ions in the mitachondria of the cell. The amount of electrical conductivity depends on the amount of electrical charges and their mobility. The electrical conductivity of living tissues is determined by the concentration of ions and their mobility, which is different in different tissues, and therefore, biological objects have the properties of conductors, semiconductors and dielectrics. The intercellular fluid saturated with ascorbic acid contains the maximum content of ions and the specific electrical conductivity of tumor tissues is high and is more than 1 S⋅m-1. Large protein molecules have a lower electrical conductivity, up to 0.003 S⋅m-1. Intracellular membranes have a conductivity below (1-3⋅10 ^-5 ) S⋅m-1. The highest values of electrical conductivity in the human body are in liquid media (blood, lymph, bile, urine, cerebrospinal fluid 0.6-2.0 cm⋅m-1) and muscle tissue (0.2 cm⋅m-1). Bone, adipose and nervous tissues have the lowest electrical conductivity, especially coarse-fibrous connective tissues and tissues of tooth enamel (10 ^-3 -10 ^-6 S⋅m-1). The electrical conductivity of cells and tissues at high-frequency and microwave currents is much more complex. In this case, biological objects have both conductivity and a capacitive connection, which characterizes the dielectric constant. The frequency dependence of the electrical parameters and the absorption of the energy of the electromagnetic field are determined by the size and shape of cells, the value of their permeability, the ratio between the volume of cells and intercellular spaces, the concentration of free ions in cells and the content of free water in them. All these factors lead to a change in the electrical conductivity of biological objects. A particularly significant factor for the metabolism of cancer cells is the content of glucose or its substitutes. In this case, ascorbic acid. If there are malignant tumors and metastases in the human body that actively and intensively assimilate glucose or its substitute - ascorbic acid, they are converted to ATP in cancer cells much lower than in healthy ones, as a result of which cancer cells become very hot and raise the temperature of the human body by 1-2 ° C. This physiological mechanism induces an increase in the temperature of tumor and surrounding normal tissues. The total rise in temperature, at present, is recorded by a microwave radiometer, which makes it possible to determine the temperature in the deep layers of the human body.

This process was partially studied by us when exposed to a biological object with tumor tissues, which were subjected to daily complex action of a constant magnetic field with an intensity of 25 μT and an alternating magnetic field with a frequency of 3.1 Hz and an intensity of 5 μT, exposure for 60 minutes a day at a time, for 5 days. The proposed method of exposure to constant and variable effects of a magnetic field on ion exchange in the mitochondria of cells and on negatively charged electrons on reticulums and cell nuclei made it possible to induce the death of tumor cells using magnetotherapy, which by 40%, compared with the control, freed biological objects from tumor cells. cells (patent No. 2307681, authors: Tsuglenok N.V., Sergeeva E.Yu., Klimatskaya L.G. RU). Therefore, this direction of using magnetic and electromagnetic fields and their effect on the energy of tumor cells deserve special attention, confirmed by researchers from South Korea, who suggested using a powerful magnetic field to destroy tumor cells. In a powerful magnetic field, the tumor begins to kill itself.

A known method of destroying cancer cells with microwave irradiation (RF Patent No. 2174021, IPC A61N 5/02) before exposure to hyperthermia, the tumor is exposed to microwave radiation with a wavelength of 1.3-2 cm and the value of the resonant frequency is absorbed by the tumors. After that, a similar effect is carried out on healthy tissues bordering with the tumor and the value of the resonance frequency of absorption of these healthy tissues is determined. Simultaneously with hyperthermia, the values of resonance frequencies of energy absorption by tumors and healthy tissues are monitored, and when the values of resonance frequencies approach, energy absorption by tumors and healthy tissues is judged on the effectiveness of treatment. This method allows to increase the efficiency of tumor treatment by microwave hypothermia when heated to 43 ° C.

The main disadvantage of this method is the strong absorption of microwave radiation by water molecules and a small difference in the heating of tumor and healthy tissues.

A known method for the destruction of cancer cells of tumor tissues (RF Patent No. 2106159 IPC A61N 5/02, A61N 5/6) the essence of the invention includes the introduction of ferromagnetic particles into the tumor localization area, followed by induction local heating, in the temperature range from 42 ° C to 45 ° C, in the course of time, determined by the type of tumor, its size, localization and the type of ferromagnetic particles selected for induction heating, while heating is carried out only at the moments of decrease in tissue blood filling, i.e. at the moments of exhalation and diastope of the patient's heart. The heating range is controlled by a microwave deep thermometer, and heating is carried out automatically, with the help of a computer, in the biocontrol mode, according to the algorithms of the mathematical model of fluctuations in the thermal conductivity and heat capacity of the tissue, the hysteresis of heating and heat removal.

The main disadvantages of this method are the low localization of magnetic particles in the tumor and the difficulty of maintaining a fixed temperature in different spatial regions of the tumor, which does not lead to a complete cure of patients.

A known method of destruction of cancerous tumors using magnetic nanoparticles (Presentation of a new magnetic field therapy system for the treatment of human solid tumors with magnetic fluid hyperthermia. Andreas Jordan, Regina Scholz, Klaus Maier-Hau, Manfred Johannsen, Peter Wust, Jacek Nadobny, Hermann Schirra, Helmut Schmidt, SerdarDeger, Stefan Loening, Wolfgang Lanksch, Roland Felix. Journal of Magnetism and Magnetic Materials 225 (2001) 118-126).

The destruction of cancer cells is based on thermolysis of magnetic nanoparticles injected into the tumor and their induction heating in an alternating magnetic field at frequencies of 50-100 kHz.

However, this method does not allow local destruction of cancer cells and requires powerful electromagnets with currents of tens of kA at relatively high frequencies. In addition, powerful alternating magnetic fields can affect the processes of movement and diffusion of ions through cell membranes, as well as generate inductive alternating electric fields that affect the operation of neural networks in the human body, associated with heating not only magnetic particles, but all cells. located in the area of injection of magnetic particles, and a strong spatial inhomogeneity of the heating temperature both inside the tumor and healthy tissues, damaging them and does not guarantee the complete death of tumor cells.

The known method of close-focus X-ray therapy with a total focal area of 100-120 Gy and remote gamma therapy for radiation destruction of malignant cells with a total focal area of 30-40 Gy (see Sh.Kh. Gantsev. Oncology, M .: Medical Information Agency. 2004, pp. 190-204; Stephen J., Withrow E., MacEwen G. Smalanimalclinicaloncology - 2001, p.305-308).

However, this method, despite its prevalence, has the following disadvantages. In the treatment of certain types of malignant neoplasms, for example melanoma, with the help of remote gamma therapy, even in combination with immunotherapy, experience shows that it leads to 75-90% of tumor recurrence, and after 2-6 months metastases occur.

The known method of neuron - capturing selective destruction of melanoma (see V. N. Mitin, N. G. Kozlovskaya, AM Arnopolskaya Neuron-capturing therapy of tumors of the oral cavity in dogs. All-Russian veterinary journal. 2006. No. 1, pp. 9-10) ...

The method involves the intravenous injection of L-borphenylalanine into the blood, which selectively accumulates in a specific tumor - melanoma, since L-phenylalanine is an essential amino acid from which melanin is produced, which forms melanocytes contained in melanoma cells. Thus, there is a selective accumulation of L-borphenylalanine in melanoma cells. When a spatial zone comparable to a tumor containing L-borphenylalanine is irradiated by a beam of slow neurons obtained through a neuron guide from a nuclear reactor, melanoma cells are destroyed due to induced secondary local boron radiation.

However, this method has the following disadvantages:

1. Radiation exposure of patients, which is only partially reduced by using a lithium protective apron.

2. A complex and very expensive installation, including a compact nuclear reactor, requiring qualified non-medical specialists, in particular nuclear physicists, to service.

3. Long-term irradiation of patients within an hour while monitoring the cardiovascular system.

4. Application of general anesthesia.

A known method of photodynamic destruction of tumors, including intravenous administration of a photosensitizer and irradiation of the tumor with continuous laser radiation with a wavelength coinciding with the absorption band of the photosensitizer (see Photodynamictherapy / Ed.TJ Dougherty / J. Clin.Laser MedSurg. 1996, Vol. 14, P 219- 348; RF Patent No. 2184578, IPC A61N 5/06). The selective photodynamic mechanism of destruction of cancer cells is based on a higher density (contrast) of the accumulation of the photosensitizer in tumor cells as compared to healthy cells, which is associated with a high density of blood vessels in a tumor as compared to healthy biological tissue.

However, this contrast for various tumors does not exceed two to three times. When laser radiation is absorbed by a photosensitizer, dye molecules pass into an excited electronic state, and upon collision with oxygen molecules dissolved in biological tissue, they transfer it from an unexcited to an excited electronic singlet state, with a typical lifetime of several microseconds. During this time, singlet oxygen molecules, having traveled a characteristic path commensurate with the size of cells when interacting with the plasma membrane of the cell, damage it, and the cell dies due to necrosis. Thus, the destruction of cells occurs only during exposure to laser radiation in the spatial area of laser irradiation.

The photodynamic method for the destruction of cancer cells has several disadvantages. Used in practice photosensitizers-phthalacionines. porphyrins, chlorins have absorption bands of photosensitizers in the ultraviolet or visible region of the spectrum, and the lasers used cannot effectively penetrate to a depth of less than a few millimeters. In addition, the photodynamic method has a low contrast of the accumulation of photosensitizers in cancer cells.

The closest to the claimed method is the destruction of biological tissue, which consists in the introduction of ethanol into it using a hollow game, characterized in that 95% ethanol is introduced in an amount equal to half the volume of biological tissue to be destroyed, then 5 ml of 20-30% ethanol is introduced, after which is carried out by heating with a high-frequency current with the simultaneous introduction of 20-30% ethanol in an amount equal to the volume of biological tissue to be destroyed. The device contains a high-frequency current generator with two cylindrical electrodes located coaxially relative to each other, internal in the form of a hollow needle through which ethanol is injected into the tumor (Abstract No. 2006113533 of the RF patent application). The disadvantages of this method include: the unreasonableness of selective absorption of ethanol by cancer and healthy cells, the difficulty of inserting a coaxial electrode into heterogeneous tumors, to organize uniform heating of tumor tissues not equally located from the exposed end of the needle.

The objective of the present invention is the local selective destruction of malignant tumors deeply located in human biological tissues, pre-selectively saturated for 3 hours with ascorbic acid, with simultaneous selective HF and microwave heating of tumors saturated with ascorbic acid, and with secondary reverse heating, after switching off the HF and Microwave energy supply from tumor tissues.

According to studies carried out on HF and UHF hyperthermia of tumor tissues, at a temperature of 43 ° C, the border between the zone of necrosis and healthy tissue is several cells. The zone of destruction of tumor tissue includes a small zone of the periphery of normal healthy tissues, which excludes degenerating cells from metastasis through their secondary necrosis from tumor tissues.

The proposed method for initiating the death of tumor cells with high-frequency and microwave energy, including the introduction of 5-10% sodium ascorbate solution into a vein of a human, or 200-400 mg orally for maximum accumulation of ascorbic acid in tumor tissues, characterized in that the initiation of selective death of tumor cells is carried out preliminary by transferring a person to a carbohydrate-free diet for 3 days, to create glucose "starvation" of cancer cells and to separate the dielectric properties of tumor and healthy tissues several times, a person is taken ascorbic acid, and 4 hours after taking photo-wave HF and microwave selective electromagnetic hyperthermia of tumor tissues, with a heating rate of tumor tissues of 0.017 ° C / sec, for 360 sec up to a temperature of tumor tissues of 43 ° C, when heating healthy tissues no higher than 40 ° C.

To maximize the separation of the dielectric properties of tumor and healthy tissues, a megadose of ascorbic acid of 12% sodium ascorbanate solution is taken, with the introduction of the solution into a vein or orally up to 500 mg of ascorbic acid, and heating and hyperthermia of tumor tissues saturated with a solution of ascorbic acid is produced by HF and microwave energy of wave radiation, in accordance with the depth of penetration of the electromagnetic wave into biological tissues and with the depth of the location of tumor tissues, at the permitted frequencies: f = 13.56 MHz - 1100 cm; f = 27 MHz - 545 cm; f = 40.68 MHz - 370 cm; f = 433.92 MHz - 34.5 cm; f = 915 MHz - 16.5 cm and f = 2450 MHz - 6.1 cm.

The physical nature of microwave radiation is the physical field of moving electric charges, in electric and magnetic fields, which are a single electromagnetic field (EMF), characterized by the oscillation frequency f. The only difference is in the frequency with which electromagnetic oscillations of the corresponding wavelength occur. The biological effect of EMF on a living organism consists in the absorption of energy by biological tissues characterized by biophysical parameters - dielectric constant and conductivity.

The tissues of the human body, due to their high water content, should be considered as dielectrics with losses. With general body irradiation, the EMF energy penetrates to a depth of 0.5 wavelengths. The intensity of exposure, exposure and dielectric losses and conductivity characterize the selective absorption of EMF by different tissues at the same density of EMF radiation.

where, λ is the wavelength;

c is the speed of propagation of the electromagnetic wave;

f is the frequency of oscillations of the electromagnetic field.

The frequency with which the electromagnetic field fluctuates significantly affects the depth of penetration of the electromagnetic wave into a biological object.

The reason lies in the commensurability with different physical objects. At f = 13.56 MHz, the EMF wavelength λ = 22 m, at f = 40.68 MHz, the EMF wavelength λ = 7.4 m, at f = 433.92 MHz, the EMF wavelength λ = 69 cm, at f = 915 MHz, EMF wavelength λ = 33 cm, and at f = 2450 MHz, EMF wavelength λ = 12.2 cm. (Table 1)

This determines the choice of equipment for local hyperthermia of tumors located at different depths in biological objects.

Tumor tissues saturated with ascorbic acid are 8-10 times higher than its content in healthy tissues, respectively, its electrical conductivity differs so many times, i.e. the ability of tumor tissues to conduct an electric current is due to the presence of an acidic electrolyte in tumors, free charge carriers - electrically charged particles that, under the influence of an external electric field in the tumor, create a conduction current.

Another important parameter of dielectric and semiconductor materials, which tumors are, are dielectric losses; they serve to determine the electrical power expended on heating dielectrics and semiconductors in an electromagnetic field. In the reference literature, to characterize the ability of a dielectric to absorb the energy of an alternating electric field, the tgδ of the dielectric loss angle and the dielectric constant ε are used. The physical meaning of tanδ consists in the presence of dielectric losses leading to a phase shift between current and voltage, where the angle between them becomes less than 90 ° by an amount, the quantitative losses of wave energy turn out to be proportional to the dielectric losses ε tgδ

Conductivity losses in dielectrics with low specific volume resistivity, for example, are absolutely chemically pure water. In nature, water is an excellent solvent and dissolves acids well, and therefore the electrical conductivity of such water has a large number of charged ions, which, under the influence of an alternating electric field, begin to move in time with the changing wave electromagnetic field, converting electrical energy into thermal energy. Tumor tissues are maximally saturated with AA, in this case they are semiconductors containing several times more charged ions in comparison with the surrounding healthy tissues and, accordingly, their heating rate is many times higher than that of the surrounding healthy tissues at the same time. In such tumor tissues, relaxation dielectric losses are additionally observed due to the rotation of polar water molecules in the direction of the lines of force of the electric field. Intramolecular friction arises, which once again increases the heating of tumor tissues.

The specific power of dielectric losses per unit volume of the dielectric is called dielectric losses, which can be calculated by the formula.

P _beats = E ² fεtgδ, 10 ^-12 W / cm ³

This ratio determines the degree of heating of various structures of tumor and healthy tissues of a biological substance in an electric field. To do this, it is necessary to know ε and tgδ of tumor and healthy tissues, and thus very accurately calculate the heating rate to a given heating temperature of tumor and surrounding healthy tissues in a uniform electromagnetic field (EMF).

Selective absorption of AA by tumor tissues leads to their selective heating of tumors and electromagnetic photoluminescence up to a higher temperature of 50 ° C when heating the surrounding healthy tissues up to 40 ° C during the same time, which leads to inoculation of tumor tissues and their subsequent destruction, which then, within a few days, they are painlessly excreted by the body. The rate of heating by the wave energy of the electromagnetic field depends on the power of the dielectric generators and magnetrons.

Knowing the specific power P _beats released in a biological object, taking into account ε and tgδ of tumor and healthy tissues, it is possible to very accurately calculate the heating rate to a predetermined heating temperature of tumor and surrounding healthy tissues in a uniform electromagnetic field (EMF). according to the formula:

Ore _op = E ² fε _op tan _op δ, 10 ^-12 W / cm ³

With the oscillatory power of the generators of an electromagnetic field of 700-850 watts, 200-300 grams of tumor tissues can be heated to a temperature of 60 ° C in 2-3 minutes, the specific power released in the tumors and their heating temperature is determined by the formula:

where Co is the heat capacity of the tumor, feces;

m is the mass of the tumor in grams;

ΔТ - heating temperature difference;

t - heating time, sec.

This formula allows you to select the required total specific power P _{beats about} for HF and microwave heating of tumor tissues P _{beats op} to a given heating temperature difference and the specific power P _beats released in healthy tissues determined by the general formula:

R _{beats about} = R _{beats op} + R _{beats zd}

Then the specific power in the field of irradiation, taking into account the dielectric properties:

_Of P _ud = (E ² fε tgδ _{op op} + E ² fε _{zd zd} tgδ) 10 ^-12

Knowing the dielectric properties of tumor ε_op tgδ_op and healthy tissues ε_bld tgδ_bld, it is possible by calculation to determine the temperature of their heating ΔT to the required specified temperatures and determine the heating time t and the total specific power P_{oud about}, the irradiated area. (Table 2)

Similarly, knowing the dielectric parameters εtgδ and the specific

the density of tumor tissues in biological objects γ g / cm ³ , it is possible to calculate the specific power released in tumor tissues P _{beats op} , saturated with various electrophotosensitizers, and determine the set temperature and calculate the time of their heating with HF and microwave energy, according to the above formulas.

Claims (1)

A method for initiating the death of tumor cells, intended for the complex treatment of cancer patients with tumor tissues in all organs of the human body, by means of their hyperthermia with HF and microwave energy, characterized by the fact that a person is transferred to a carbohydrate-free diet for 3 days to create glucose starvation and the subsequent maximum saturation of oncocells with an electron-ionic solution of ascorbic acid in a one-time megadose of 350 mg, and after 4 hours with a maximum accumulation of the drug in tumor tissues 2-4 times higher than in healthy ones, selective hyperthermia of tumor tissues with HF energy is carried out in accordance with with a depth of their location and the depth of penetration of an electromagnetic wave into the human body of 1100 cm, at an allowed frequency f = 13.56 MHz, with a total heating rate of tumor tissues at these frequencies of 0.017 ° C / s, for 360 s to a temperature of tumor tissues of 43 ° C, when heating healthy tissues not higher than 40 ° C.