RU2672122C2 - Method of modeling postoperative purulent-inflammatory complications in the experiment - Google Patents

Abstract

FIELD: medicine.SUBSTANCE: invention relates to experimental medicine and concerns the modeling of postoperative purulent-inflammatory complications. To do this, a rat under ether anesthesia, while observing the rules of aseptic and antiseptic, creates a middle wound of the abdomen. Incision is made from the xiphoid process to the frontal symphysis, while dissecting the skin, subcutaneous fatty tissue and aponeurosis. Withstands the wound cavity in an unclosed condition for 20 minutes, followed by suturing the wound. Then every day for 5 consecutive days, the procedure for the removal of stitches is performed and when the edges of the stupidly diluted edges of the wound survive the cavity of the wound under similar conditions. At the same time, aseptic and antiseptic rules are followed, and after suturing the wound.EFFECT: method ensures the creation of a model with the most adequate pathogenesis of postoperative purulent-inflammatory complications, with a decrease in the mortality of experimental animals and the risk of other postoperative complications.1 cl, 2 ex

Description

The invention relates to medicine, namely to purulent surgery, pathological physiology and experimental medicine. It can be used to study the pathogenesis of postoperative purulent-inflammatory complications and preclinical studies of the effectiveness of new drugs and prevention methods for this pathology.

A known method of modeling postoperative purulent-inflammatory complications in an experiment is that a rat under ether anesthesia, subject to all the rules of aseptic and antiseptic, performs laparotomy, incubated for 20 minutes, then the laparotomy wound is sutured, the relaparotomy operation is repeated daily for 5- for days, each time the sutures of the laparotomic wound are removed and the edges of the wound are stupidly spread for 20 minutes (Popov P.V., Ways to study the effectiveness of various antibiotic prophylaxis schemes of postoperative purulent-septic complications and drainage of the lymphatic system in the experimental postoperative period // Bulletin of Experimental and Clinical Surgery Volume VII, No 2 2014. - P. 115-118., Popov PV, Syropyatov B.Ya., Odegova TF Modeling of postoperative complications for studying of efficiency of preventive antibiotics in perioperative period Science and Education [Text]: materials of the II international research and practice conference, Vol. II, Munich, December 18 ^th -19 ^th , 2012 / publishing office Vela Verlag Waldkraiburg - Munich - Germany, 2012 - p. 469-471).

The disadvantages of this method are the high mortality and the development of multiple organ failure in experimental animals, especially after 4 days, when the appetite deteriorated in rats against the background of general lethargy.

The proposed method is as follows. A rat under ether anesthesia, subject to all the rules of asepsis and antiseptics, produces a median wound over the projection of the white line of the abdomen to the peritoneum, i.e. skin, subcutaneous fatty tissue and aponeurosis are dissected. Further, the open wound is held for 20 minutes, then it is sutured, the operation must be repeated daily for 5 days.

Effect: creating a model with the most adequate pathogenesis of postoperative purulent-inflammatory complications; reduced mortality of experimental animals; reduced risk of developing other postoperative complications.

All experiments were conducted in accordance with the "Rules for the Use of Experimental Animals" (Appendix to the Order of the Ministry of Health of the USSR dated 08.08.1977 No. 755) and the "European Convention for the Protection of Vertebrate Animals Used for Experiments or for Other Scientific Purposes" from March 18, 1986 All experimental animals contain vivarium under standard conditions: standard content in plastic cages with fine wood shavings, standard diet of vivarium.

Under ether anesthesia, the animal on the back for legs is attached to the operating table, on the stomach, wool is removed. The surgical field is treated with 5% alcohol solution of iodine, a wound is produced from the xiphoid process to the pubic symphysis, while dissecting the skin, subcutaneous fat and aponeurosis. They stand for 20 minutes, sew up the postoperative wound with single-layer single sutures, and re-treat the sutured wound with a 5% alcohol solution of iodine with the obligatory observance of all the rules of asepsis and antiseptics. Every day, the edges of the wound stupidly bred after removing the stitches, 5 days in a row, in similar conditions. On the second or third day, the edges of the wound stop bleeding, which indicates a deterioration in capillary blood supply, the peritoneum acquires a dim light. On the fourth day, the wound edges are covered with fibrin, and on the fifth day a purulent exudate appears in the wound, which is fully consistent with the modeling of postoperative purulent-inflammatory complications in the experiment.

All animals in the postoperative periods independently ate and maintained healthy activity for the entire period of the experiment. In a bacterioscopic examination of pus from wounds, a multivalent culture is determined with a predominance of staphylococci and Escherichia coli.

Case Studies

Example 1. A male Wistar rat weighing 120.0 g under ether anesthesia on the back behind the legs was fixed on the operating table, on the abdomen shears were cut with wool. The surgical field was treated with 5% alcohol solution of iodine, a wound was made from the xiphoid process to the pubic symphysis with a sterile instrument, dissecting the skin, subcutaneous fatty tissue and aponeurosis. The wound cavity was kept open for 20 minutes, then sutured with single-layer single sutures, the sutured wound was re-treated with 5% alcohol solution of iodine, all the rules of aseptic and antiseptic were strictly observed. Every day, the edges of the wound stupidly parted after removing the stitches, 5 days in a row, under similar conditions. On the fifth day, when pressing with a spatula, a whitish-yellow thick pus stood out on the wound.

Example 2. Female rats of the Wistar strain weighing 150.0 g under ether anesthesia on their backs were fixed on the operating table by the legs, and their hair was cut with scissors on their stomach. The surgical field was treated with a 5% alcohol solution of iodine, a middle wound was made with a sterile instrument from the xiphoid process to the pubic symphysis, while dissecting the skin, subcutaneous fat and aponeurosis. The wound cavity was kept open for 20 minutes, then it was sutured with single-layer single sutures, the sutured wound was re-treated with 5% alcohol solution of iodine, all the rules of asepsis and antiseptics were strictly observed. Reoperation after removal of the sutures and blunt dilution of the edges of the wound was performed daily, similarly, for 5 consecutive days. On the fifth day, when pressing with a spatula on the wound, whitish pus with a green tint stood out.

Claims (1)

A method for modeling postoperative purulent-inflammatory complications in an experiment, including creating a wound to the abdomen of a rat under ether anesthesia subject to the rules of aseptic and antiseptic, keeping the wound cavity in a non-wound state for 20 minutes, then suturing the wound and then conducting the procedure daily for 5 consecutive days removal of sutures and with obtuse dilution of the edges of the wound, maintaining the wound cavity under similar conditions, characterized in that they produce a median wound to the peritoneum from the xiphoid process frontal symphysis, while slicing the skin, subcutaneous fat and aponeurosis, respecting the rules of asepsis and antisepsis and after suturing the wound.