RU2663463C1 - Method for increasing effectiveness of application of nutrient stimulants in fattening of young cattle - Google Patents

Abstract

FIELD: medicine; veterinary medicine; agriculture.

SUBSTANCE: method for increasing effectiveness of the use of nutrient stimulants in fattening young cattle, including subcutaneous administration of solution of preparation “Nucleopeptide” with electrochemically activated aqueous solution of catholyte with parameters Eh = -300 mV and pH 7-8, glycine-stabilized amino acid at a concentration of at least 0.01% by weight, with volumetric quantitative ratio of preparation: a catholyte solution of 5:1, but not more than 30 ml in a dose of 0.14 ml/kg of live weight for 3 consecutive days from 6 months of age, then once every 15 days.

EFFECT: invention provides improvement of growth and development of young cattle.

1 cl, 7 tbl

Description

The invention relates to medicine, veterinary medicine, agriculture and other industries.

It is known about the stimulating effect of tissue preparations (biogenic stimulants), in particular from the spleen, on the growth and development of calves by its parenteral administration (subcutaneously) [1, 2, 3, 4, 5].

However, the effect of this drug, prepared according to the method of V.P. Filatov in a neutral saline solution does not allow to fully realize the possibility of increasing resistance and increasing the gain of young animals. On the other hand, to enhance the action of biologically active compounds of the drug in the prototype [5], the phenomenon of synergism that enhances activity is not used.

"Nucleopeptide" is a purified cattle spleen extract obtained by tissue autolysis, contains low molecular weight peptides of at least 1 mg / ml.

Our proposed method of using the drug “Nucleopeptide” (certificate No. POCC RU. VP01.N00677) [6] provides for its subcutaneous injection to use an increase in efficiency in the ratio of 5: 1 with an electrochemically activated catholyte solution with parameters Eh = -300 mV and pH 7- 8, stabilized by the amino acid glycine in a concentration of not less than 0.01 wt. % possessing a potentiating synergistic effect [7].

An aqueous electrochemically activated stabilized solution can be used as an integral part of a complex drug composition by subcutaneous administration of the Nucleopeptide biopreparation, which gives it the properties of a disinfectant, antiseptic, preservative, antioxidant, antimutagen, radioprotector, immunostimulant, adaptogen, antiviral composition, antiviral composition, antiviral composition tissue regeneration stimulator and / or mitotic activity of bacterial microflora useful for humans and / or animals gastrointestinal tract [7].

Injections of the biological product “Nucleopeptide” to the fattening young cattle were carried out taking into account the instructions of the manufacturer LLC “Ecohimtech” (Ufa) [8].

The duration of subcutaneous injection of the drug is not more than 6 months, the recommended interval between injections is 2-3 months, the repeated cycle of use is 2-3 months, subcutaneous injections are administered in a dose of not more than 30 ml.

The electrochemical activation of the starting tap water was carried out at ESPERO-1 manufactured by a Tashkent company and widely advertised on the Internet with an optimal electrolysis period of 10-15 minutes.

The studies were carried out in accordance with the experimental design (table. 1).

To conduct research at the age of 5 months, on the basis of the analogue groups principle, 5 groups of black-motley bull-calves of 10 animals each were formed. The drug was not administered to the calves of the control group, the animals of the experimental group were injected with the Nucleopeptide subcutaneously in the middle third of the neck at a dose of 0.14 ml / kg body weight 3 days in a row from 6 months of age 1 time in 15 days, but not more than 20 ml Experimental II, respectively, in the same dose, but not more than 25 ml, Experimental III, in the same dose, but not more than 30 ml, to the bulls of the IV experimental group while maintaining the dose of the drug injection of 0.14 ml / kg live weight in its solution first we introduce an electrochemical activated (ECA) solution of catholyte with parameters Eh = -300 mV and pH 7-8, stabilized amino acid glycine in a concentration of not less than 0.01 wt. % with a volumetric quantitative ratio of the drug: catholyte 5: 1, but not more than 30 ml (table. 1).

The diets of the experimental animals were balanced according to detailed feeding standards.

At the beginning, middle and end of the experiment, hematological parameters were studied in three animals from each group.

In order to study the meat productivity and the quality of the pulp, a control slaughter of three bulls from each group was carried out.

Assessing the ability of the compared groups of animals to digest basic nutrients, the subcutaneous administration of the Nucleopeptide positively affected the digestibility ratios of nutrients in diets (Table 2). Among the groups of gobies that received only a biostimulant injection, the advantage was on the side of animals of group II. They exceeded peers of groups I and III in terms of dry and organic substances by an average of 1.78 and 1.35%, crude protein by 2.0 and 0.24%, raw fat by 1.55 and 0.43%, crude fiber - by 0.87 and 0.56%, BEV - by 0.9 and 0.21% (Table 2).

However, the digestibility indices of group IV gobies, who received an injection of the same dose of the drug no more than 25 ml / goal with stabilized catholyte in the ratio 5: 1, were higher on dry and organic substances - by 1.67%, crude protein - by 3.95% , crude fat - by 3.52%, crude fiber - by 4.09% and BEV - by 1.63% (Table 2).

If from the data table. Figure 3 shows that the average live weight of gobies of all groups when setting up the experiment was approximately the same (171.7; 178.2 kg), then at 9 months of age, intergroup differences were noted. At the age of 15 months, young animals in the control group were inferior to peers of group II by 48.2 kg (12.6%), and IV - by 69.6 kg (18.3%). A similar picture was observed in the final period of fattening of young animals of all groups.

A more visual representation of the growth and development of young groups is given by the indicators of average daily growth and relative growth rate - table. four.

If the bulls of group II exceeded the peers of the control group in terms of average daily growth by 78 g (11.59%), with a relative growth rate of 93.1%, the result of the proposed method in group IV was higher - by 146 g at a growth rate of 93, 5%.

When studying the morphological and biochemical composition of blood, some of its features were established - table. 5. The data obtained indicate the influence of the seasons on the studied indicators. The increase in erythrocyte content in the summer period compared to the winter period averaged 10% in the groups, hemoglobin concentration - 12% and total protein - 3.1%, which is consistent with the nature of the increase in the growth rate of young animals over the periods of the year.

Similar patterns were observed in the activity of transamination enzymes - ACT and ALT. The greatest increase in enzymes in the summer is due to a more active metabolism in this season of the year.

An objective assessment of the high level of meat productivity of experimental bulls was obtained during control slaughter (Table 6). In terms of pre-slaughter live weight, the superiority of bulls of group I over the control was 1.8 kg (0.46%), group II 28.2 kg (5.91%), group III 4.0 kg (0.9%) and IV - 51.6 kg (11.6%). The heaviest carcasses were obtained from young animals of group IV. The large mass of the pair of carcasses of gobies of group IV caused the increase in slaughter yield by 5.95% - table 6.

When assessing the quality of meat products by the ratio of protein and fat in the average sample of meat of gobies of all groups 1: 0.54, which indicates the high nutritional and energy value of meat of gobies of experimental groups.

Thus, fattening young cattle of black-motley breed are injected subcutaneously from the age of 6 months with the biologically active substance “Nucleopeptide” in a mixture with an electrochemically activated aqueous solution of catholyte at a component ratio of 5: 1 at a dosage of no more than 30 ml / goal . cost-effective - table. 7. Additional profit from the implementation is 526.9 rubles / goal. With a profitability level of 52.5%.

Information sources

1. Chamberev Yu.N. The effect of hormonal and substrate preparations on the growth, metabolism and adaptive abilities of animals // Bulletin of the Timiryazev Agricultural Academy. - 2007. - No. 4. - S. 111-121.

2. Kiseleva M.V. The effect of anti-stress drugs and growth stimulants on the meat productivity of bulls of the Hereford breed // Zootechny. - 2008. - No. 2. - S. 21-22.

3. Pogodaev V. A., Mambetova M. M., Mambetov M. M. Productivity indicators of young buffalo when using a biogenic stimulator from drone brood (sitra) // Zootechny. - 2012. - No. 3. - S. 12-14.

4. Frank A.M., Frolov A.I., Balobaev R.V., Lobkov V.Yu. The effectiveness of the use of an immune biostimulator based on hydrolyzed vegetable protein and organic trace elements in cattle diets // Vestnik APK Verkhnevolzhie. - 2014. No. 1. - S. 45-50.

5. Ibatova G.G., Vagapov F.F. The meat productivity of gobies with intensive cultivation using a nucleopeptide growth stimulator // Bulletin of the Samara State Agricultural Academy. - 2015. - No. 1. - S. 119-121 - prototype.

6. Certificate of conformity No. ROSS. RU. VP 01.N 00677 dated 12/14/2012. Products: Nucleopeptide - to increase the resistance and development of young farm animals.

7. Patent for invention RU No. 2234945: Stabilizer of an aqueous solution and water-containing raw materials with spontaneously changing redox properties / V.M. Janitors: published on August 27, 2004.

Claims (1)

A method for increasing the efficiency of using biogenic stimulants for fattening young cattle, including subcutaneous administration of a solution of the Nucleopeptide preparation with an electrochemically activated aqueous solution of catholyte with parameters Eh = -300 mV and pH 7-8, stabilized by glycine amino acid at a concentration of at least 0.01 mass %, with a volumetric quantitative ratio of the drug: catholyte solution 5: 1, but not more than 30 ml at a dose of 0.14 ml / kg body weight 3 days in a row from 6 months of age, then 1 time every 15 days.