RU2644193C1 - Method for management of aerobic microorganisms biomass production - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: method for management of aerobic microorganisms' biomass production is proposed. The method includes setting the steam generator output at the ejector inlet, steam ejector refrigeration machine ejection rate, the measured values of inlet and outlet water temperatures of the cold receiver, coolant temperature in the cold receiver and the inlet water flow to the cold receiver are used to determine the value of the heat transfer coefficient from the coolant to water and control the temperature of cold water by influencing the heat transfer coefficient. If the temperature of cold water supplied to the inoculant cooling jacket and the finished culture collectors cooling system is higher than 10°C, the heat transfer coefficient is increased, and if the cold water temperature deviates below 7°C, the heat transfer coefficient is reduced, and the temperature of the warm water supplied from the condenser to the fermenter jacket is set and the concentration of the finished culture is stabilized according to the optical density of the finished culture.

EFFECT: increased accuracy and reliability of management of anaerobic microorganisms biomass production, reduced specific energy inputs and increased yield of the finished product.

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Description

The invention relates to the automation of technological processes and can be used to automate the process of aerobic deep fermentation when growing cultures of microorganisms and producers of enzymes in the microbiological, medical, pharmaceutical and food industries.

There are various methods for the production of biomass of aerobic microorganisms [US Pat. RF 2021351 C1, C12N 5/00, C12C 3/06; Pat. RF 2021353 C1, C12N 5/00, C12C 3/06; Fermentation apparatus for microbiological synthesis processes / A.Yu. Vinarov, L.S. Gordeev, A.A. Kukharenko, V.I. Panfilov // M .: DeLi print, 2005 .-- 191 p., Pat. RF 2322488 C2, C12N 1/00, C12M 1/00, publ. 04/20/2008].

A common disadvantage of the known methods is that they do not provide for the operational control of parameters at all stages of the production of biomass of aerobic microorganisms, which does not allow solving energy conservation problems when growing microorganism cultures with maximum recovery and utilization of secondary energy resources in closed thermodynamic cycles in material and energy flows.

A known method of controlling a continuous process for producing biomass of methane-oxidizing microorganisms [US Pat. RF 2064016, C12N 1/00], carried out in a fermenter with an adjustable volume, pH and temperature of the growing medium; regulated supply of all components of the nutrition of microorganisms and process water; controlled selection of a suspension containing biomass from the fermenter; regulating the pressure in the fermenter and the flow of the spent medium supplied to the fermenter after concentration of the biomass; the regulated amount of gas and liquid flows containing components of microorganism nutrition with restrictions due to safety conditions and efficient energy utilization of exhaust gases; adjustable flow ratio of a biomass-containing suspension taken from the fermenter and returned to the fermenter of the spent medium after concentration, achieving an increase in the pH stabilizing flux.

However, the method does not implement the basic principles of energy conservation associated with the recirculation of coolants in closed thermodynamic cycles during their repeated use; no conditions have been created for increasing thermal efficiency, since cold and warm water is not provided for the implementation of the process of biomass production of aerobic microorganisms using heat pump technologies, in particular a steam ejector refrigeration machine operating in the heat pump mode; the possibility of maximum recovery and utilization of secondary energy resources is excluded.

The closest in technical essence and the achieved effect to the proposed is a method for the production of biomass of aerobic microorganisms [Pat. 2484129, Russian Federation, IPC ⁷ С12М 1/00, C12N 1/00, С12М 1/36. Method for the production of biomass of aerobic microorganisms [Text] / Korneeva OS, Shevtsov AA, Cheremushkina IV, Mazhulina IV, Cherenkov D.A .; Applicant and patent holder Voronezh. state University. engineering technol. - No. 2012118115/10; declared 05/03/2012; publ. 06/10/2013; Bull. No. 16], providing for the preparation of a liquid seed culture in the inoculator with a cooling jacket; growing a microorganism culture in a heating jacket fermenter; storage of the finished culture of the microorganism in the collections of the finished culture with a cooling system; preparation of cold water with a temperature of 7 ... 10 ° C and warm water with a temperature of 27 ... 47 ° C in a steam ejector chiller consisting of an ejector, an evaporator, a cold receiver, a condenser, a thermostatic valve, a waste water collector, a steam generator with electric heating elements and a safety valve, a pump for supplying water to a steam generator, a refrigerant recirculation pump through a cold receiver, operating in a closed thermodynamic cycle; the use of a portion of the steam obtained in the steam generator for pre-sterilization of the inoculator, fermenter and collections of the finished culture; the supply of “cold” water to the cooling jacket of the inoculator and the cooling system of the collections of the finished culture and “warm” water to the heating jacket of the fermenter with the formation of recirculation circuits for material and heat flows.

The known method does not provide accuracy and reliability of control of technological parameters, does not allow to increase the yield of finished biomass with maximum activity due to the lack of a stabilization system for abiotic factors, and, first of all, the concentration of the resulting microbial biomass under random disturbances caused by a possible uneven distribution of the gas phase over the whole mass of culture in the fermenter and technological malfunctions in the work of auxiliary equipment.

The method does not provide for the use of operational information from the control object for regulating the temperature conditions of continuous aerobic cultivation of biomass in the fermenter, stabilizing the storage conditions of the finished culture in storage tanks, as well as for preparing coolants of different temperature potentials in a steam ejector refrigeration machine operating in the heat pump mode, which does not guarantee a change in the parameters within the specified values and does not create optimal conditions for the growth and reproduction of microns organisms with minimal energy consumption.

An object of the invention is to increase the accuracy and reliability of controlling the process of biomass production of aerobic microorganisms, reducing specific energy consumption and increasing the yield of the finished culture.

To solve the technical problem of the invention, in a method for controlling the process of biomass production of aerobic microorganisms, the preparation of a liquid seed culture in an inoculator with a cooling jacket and mixer; growing a microorganism culture in a fermenter with a heating jacket and stirrer; storage of the finished culture of the microorganism in the collections of the finished culture with a cooling system; preparation of cold water with a temperature of 7 ... 10 ° C and warm water with a temperature of 27 ... 47 ° C in a steam ejector chiller, consisting of an ejector, an evaporator, a cold receiver, a condenser, a thermostatic valve, a waste water collector, a steam generator with electric heating elements and a safety valve, a pump for supplying water to a steam generator, a refrigerant recirculation pump through a cold receiver, operating in a closed thermodynamic cycle; the use of a portion of the steam obtained in the steam generator for pre-sterilization of the inoculator, fermenter and collections of the finished culture; the supply of cold water to the cooling jacket of the inoculator and the cooling system of the collections of the finished culture and warm water to the heating jacket of the fermenter with the formation of recirculation circuits for material and heat flows, new is that the temperature of preparation of the liquid seed culture in the inoculator is measured; the temperature of the culture of the microorganism in the fermenter; steam output of a steam generator; steam consumption from the steam generator for preliminary sterilization of the inoculator, fermenter and ready-made culture collectors; optical density of the finished culture; working steam consumption in the ejector; the amount of vacuum in the evaporator and the flow rate of the ejected steam from the evaporator, the level and temperature of the refrigerant in the evaporator, the level and pressure of water in the steam generator; cold water temperature before and after the cold receiver; the flow rate of water supplied to the cooling jacket of the inoculator and the cooling system of the collections of the finished culture; temperature of the mixture of working and ejected vapors before and after the condenser; the temperature of the warm water supplied to the jacket of the fermenter; set the desired steam capacity of the steam generator at the inlet of the ejector by affecting the power of the electric heating elements of the steam generator and the ejection coefficient of the steam ejector chiller by affecting the ratio of the working and ejected steam by changing the flow rate of the ejected steam; From the measured values of the temperature of the water entering and leaving the cold receiver, the temperature of the refrigerant in the cold receiver, as well as the flow rate of cold water supplied to the inoculator cooling jacket and the cooling system of the finished culture collectors, the value of the heat transfer coefficient from the refrigerant to the water through the cooling surface of the cold receiver is determined and the regulation cold water temperature effect on the heat transfer coefficient; moreover, when the temperature of the cold water supplied to the inoculator cooling jacket and the cooling system of the finished culture tanks deviates above 10 ° C, the heat transfer coefficient is increased by influencing the increase in the refrigerant flow in the recirculation circuit from the evaporator through the cold receiver to the evaporator, and when the temperature of the cold water deviates below 7 ° C reduce the heat transfer coefficient by affecting the decrease in the flow of refrigerant in the recirculation loop from the evaporator through the cold receiver to the evaporator; set the temperature of warm water supplied to the fermenter jacket from the condenser by affecting the ejection coefficient by changing the flow rate of the working steam at the inlet of the ejector with a correction for the temperature of growing the microorganism culture in the fermenter; and stabilize the concentration of the finished culture by the optical density of the finished culture by affecting the frequency of rotation of the stirrer of the fermenter.

The technical result of the invention is to increase the accuracy and reliability of controlling the process of biomass production of aerobic microorganisms, reduce specific energy consumption and increase the yield of the finished culture.

Figure 1 presents a diagram that implements the proposed control method.

The circuit contains a fermenter 1 with a heating jacket 2, mixing devices 3 and aeration 4; inoculator 5 with a cooling jacket 6, mixing devices 7 and aeration 8; collections of finished culture 9 with a cooling system 10; a collection of waste water and condensate 11; a steam generator 12 with electric heating elements 13 and a safety valve 14; ejector 15; capacitor 16; thermostatic valve 17; evaporator 18; cold receiver 19; pumps 20, 21, 22, 23, 24; microprocessor 25; material flow lines: 0.1 - sterile air; 0.2 - exhaust exhaust sterile air; 1.0 - cold water; 1.1 - waste water; 1.2 - water in the cold receiver; 1.3 - refrigerant recirculation through a cold receiver; 1.4 - water to the condenser; 1.5 - warm water in the fermenter; 2.0 - working steam; 2.1 - a pair of inoculator and fermenter; 2.2 - ejected refrigerant vapor; 2.3 - a mixture of working and ejected vapor; 2.4 - condensate; 2.5 - pressure relief; 3.1 - nutrient medium; 3.2 - seed; 3.3 - squeezing liquid sowing culture; 3.4 - finished culture of microorganisms; sensors: TE - temperature, FE - flow; ρЕ - optical density; RE - pressure, vacuum; And - actuators.

The production of biomass of aerobic microorganisms begins with the preparation of a liquid seed culture in the inoculator 5 with a cooling jacket 6, mixing devices 7 and aeration 8.

The resulting liquid seed culture in the inoculator 5 is cultivated at a pH of 4.2 ... 4.5 and a temperature of 31 ... 32 ° C until the exponential growth phase is reached within 12 ... 36 hours, ensuring its uniformity by means of a mixing device 7.

After the cultivation time, the liquid seed culture is pushed with sterile air through the transfer line 3.3 from the inoculator 5 to the pre-sterilized fermenter 1 in the amount of 3 ... 10% of the amount of nutrient medium, which is filled with 7/10 of its volume.

In the fermenter 1, a microorganism culture is grown with a fermentation temperature of 28 ... 40 ° C for 96 ... 120 hours with continuous aeration with sterile air through an aeration device 3, mechanical stirring with a stirring device 4 and the supply of “warm” water through line 1.3 to the heating jacket 2.

After fermentation, the finished culture of the microorganism from the fermenter using a pump 20 is fed through line 3.4 to pre-sterilized collections of the finished culture 9 with a cooling system 10, where the culture temperature is maintained at the level of 8 ... 12 ° С.

To prepare cold and warm water, a steam ejector refrigeration machine is used, operating in the heat pump mode, consisting of an ejector 15; evaporator 18; cold receiver 19; capacitor 16; thermostatic valve 17; a waste water collector 11, a steam generator 12 with heat elements 13 and a safety valve 14; a pump for supplying water to the steam generator 22; refrigerant recirculation pump through a cold receiver 23 operating in a closed thermodynamic cycle.

At the same time, in the steam generator 12, by means of electric heating elements 13, sharp steam is obtained, one part of which as a working steam under pressure 0.05 ... 0.06 MPa is fed through line 2.0 to the ejector nozzle 15, involving ejected refrigerant vapors along line 2.2, as which they use water from the evaporator 18 and create a reduced pressure in it of 0.0009 ... 0.001 MPa with a boiling point of refrigerant of 4 ... 7 ° С. Due to the recirculation of the refrigerant along line 1.3, cold water with a temperature of 1 ... 10 ° C is obtained through the cold receiver 19 by means of recuperative heat exchange between the refrigerant and the water supplied through line 1.3 to the cold receiver 19 from the collector 11 using a pump 22.

Received cold water from the cold receiver 19 through line 1.0 is fed into the cooling jacket 6 of the inoculator 5 and the cooling system 10 of the collections of the finished culture 9.

The mixture of refrigerant vapor and working steam formed after the ejector 15 is sent to the condenser 16 through line 2.3. The condensation process is accompanied by the release of heat, while the condensation heat in the condenser 16 is used to produce “warm” water through the regenerative heat exchange between the water supplied from the collector 11 by the pump 24 through line 1.4 to the condenser 16, and the condensing vapors of the mixture in the condenser 16. The water heated to a temperature of 27 ... 47 ° C is fed through line 1.5 to the heating jacket 2 of the fermenter 1.

A part of the water condensate formed after the condenser 16 is sent through a thermostatic valve 17 via line 2.4 to the evaporator 18 to replenish the water loss in it, and the other excess part of the condensate is removed from the closed cycle of the steam ejector refrigeration machine and together with the waste water after inoculator 5, fermenter 1 and collectors the finished culture 9 is fed through lines 1.1 to the waste water collector 11, from which one part of the water is sent via line 1.2 to replenish the loss of water in the steam generator 12, and the other part along two streams 1.3 and 1.4 using a pump 24 serves in the cold receiver 19 and the condenser 16 of the steam ejector refrigeration machine with the formation of a closed cycle. With increasing steam pressure in the steam generator, a safety valve is activated, which relieves pressure.

Information on the preparation of the liquid seed culture in the inoculator 5, on the growth of the culture of the microorganism in the fermenter 1, as well as on the preparation of cold and warm water in the steam ejector chiller operating in the heat pump mode, is transmitted using sensors to microprocessor 18, which in it, the program-logic algorithm provides operational control of technological parameters by means of actuators, taking into account the restrictions imposed on them, due to how high Exit ready culture, and economic feasibility.

The actual steam production of the steam generator 12 is established by the microprocessor 25 by affecting the power of the electric heating elements 13 based on the required amount of steam for preliminary sterilization of the inoculator 5, fermenter 1 and finished culture collectors 9, as well as on the preparation of working steam supplied via line 2.0 to the ejector 15 of the steam ejector refrigeration machine for obtaining warm and cold water in accordance with the balance ratios from the conditions for the performance of a given capacity for the finished biomass.

In this case, the microprocessor 25 sets the coefficient of ejection of the steam ejector chiller by influencing the ratio of the flow rate of the working steam supplied to the ejector nozzle through line 2.0 and that ejected from the evaporator through line 2.2 by changing the flow rate of the working steam and controls the rarefaction and temperature of the refrigerant in the evaporator 18.

The microprocessor continuously determines the current value of the heat transfer coefficient from the refrigerant to the water through the cooling surface of the cold receiver 19 according to the formula:

where Q = Vcρ (t ₁ -t ₂ ) is the amount of heat supplied by the flow of water from the condensate collector 11 by pump 24 to the cold receiver along line 1.2, kJ / h; s, ρ - average values of heat capacity, kJ / (kg⋅K), density, kg / m ^{3 of} water; V is the volumetric flow rate of water supplied to the cold receiver, m ³ / h; F is the area of the heat exchange surface of the cold receiver, m ² ; Δt _cp = (t ₁ -t ₂ ) / ln [(t ₁ -t ₃ ) / (t ₂ -t ₃ )] - logarithmic temperature head, ° С; t _1, t ₂ - the water temperature in the line 1.2 at the inlet and outlet line of 1.0 holodopriemnika, ° C; t ₃ - temperature of the refrigerant in the cold receiver, ° C,

and monitors the temperature of cold water at the outlet of the cold receptacle 19 in line 1.0, and when the temperature of the cold water supplied to the cooling jacket 6 of the inoculator 5 and the cooling system 10 of the finished culture collector 9 deviates above 10 ° C, the microprocessor 25 increases the heat transfer coefficient from the refrigerant to water through the cooling surface of the cold receiver 19 by increasing the flow rate of the refrigerant in the recirculation circuit 1.3 from the evaporator 18 through the cold receiver 19 to the evaporator 18 by influencing the increase in the power controlled about the drive of the pump 23, and when the temperature of the cold water deviates below 7 ° C, the microprocessor 25 reduces the heat transfer coefficient by affecting the decrease in the flow of refrigerant in the recirculation loop 1.3 from the evaporator 18 through the cold receiver 19 to the evaporator 18.

The microprocessor 25 sets the temperature of the warm water supplied to the jacket 2 of the fermenter 1 from the condenser 16 via line 1.5, which is heated by means of regenerative heat exchange between the water supplied through line 1.4 and the condensing refrigerant vapors in line 2.3. By changing the coefficient of ejection of the steam ejector chiller by changing the flow rate of working steam in line 2.0 at the inlet to the ejector 15, the microprocessor provides the necessary value of the temperature of the “warm” water in line 1.5. In this case, the temperature of warm water is continuously adjusted according to the temperature of growing the culture of the microorganism in the fermenter 1. When the temperature of the growth of the culture of the microorganism in the fermenter 1 deviates both downward and upward due to possible random disturbances, the microprocessor affects the ejection coefficient, providing a temperature change warm water in the jacket of the fermenter, and stabilizes the fermentation temperature in the fermenter 1 in the set value region.

In order to effectively mix during continuous cultivation, which ensures the complete elimination of stagnant zones in the fermenter, the microprocessor continuously monitors the concentration of the finished culture in line 3.4 by its optical density and creates optimal conditions for the growth of microorganisms. The stabilization of the optical density of the finished culture by influencing the speed of the stirrer of the fermenter 1 provides sufficient contact between the oxygen of the air and the liquid medium and the best respiratory characteristics of the population cells, which leads to an increase in the biomass yield.

Information about the current value of the level of refrigerant in the evaporator 18 is continuously transmitted to the microprocessor. When the refrigerant level decreases, the microprocessor generates a signal to increase the condensate flow rate to the evaporator 10 through the thermostatic valve 17 in line 2.4 and reduces the condensate flow rate when the refrigerant level reaches the upper preset value.

Information about the current value of the level of condensate in the steam generator 12 is transmitted to the microprocessor. When the condensate level changes, the microprocessor performs on-off control by the drive of the feed pump 22, turns on the feed pump when the condensate level in the steam generator reaches the lower set value, delivers water from the condensate collector along line 1.2, and turns it off when the upper set value is reached.

When the pressure of saturated water vapor in the working volume of the steam generator 12 is increased above the permissible value, the microprocessor performs an emergency pressure relief via line 2.5 through the safety valve 14.

Examples of the implementation of the method of controlling the process of biomass production of aerobic microorganisms

The method of controlling the process of production of aerobic biomass provided for the preparation of a liquid seed culture in the inoculator in the following production cycle (in h):

Cleaning and inspection 1.0-1.5 Leak test 0.5-1.0 Pressure check and sterilization 2.5 Nutrient loading 0.5 Culture Sterilization 1,5 Crop cooling and inoculation 2.0-3.0 Growing liquid seed crops 12-36 Transfer of the crop to the fermenter 0.5

Monitoring and control of the process of growing microbial cultures in an aerobic deep fermentation fermenter with a combined supply of energy: to the gas phase for aeration with sterile air using a bubbler and to the liquid phase by mixing with a mechanical stirrer, it provides a metered flow of nutrient medium, inoculum (seed), sterile air, “warm” water into the heating jacket and high intensity of mass and energy exchange of the inoculum microbial cells with the nutrient medium due to the stable tion process parameters on the level required for optimum development and producing the target product. The exhaust air, waste water and the finished culture of microorganisms were removed from the fermenter in the form of a mixture containing cells, extracellular metabolites and biomass with a residual concentration of the target product.

The production cycle of the fermenter was:

Cleaning and inspection 1.0-1.5 Leak test 0.5-1.0 Pressure check and sterilization 1.5-2.0 Nutrient filling 4.0-4.5 Fermentation 96-120 Transfer of finished culture to consumables 0.5-1.0

The fermentation process was carried out in a vertical fermenter company "Sartorius Stedim Biotech" BIOSTAT series with a working volume of 100 l, intended for growing microorganisms or cell cultures. Control over process parameters was carried out using a microprocessor control system DCU (Digital Control Unit). To stabilize the temperature regimes when preparing a liquid seed culture in an inoculator, directly growing a culture of microorganisms in a fermenter and cooling the finished culture in receiving tanks, the preparation of “warm” and “cold” water was carried out using a steam ejector chiller operating in the heat pump mode with a technical characteristic :

Cooling capacity, kW 20-25 Boiling temperature: in the evaporator, ° С 4-6 in a steam generator, ° С 154-157 Condensation temperature, ° С 127-130 Inlet water temperature to condenser, ° С 15-18 Ejection coefficient 3.0-4.5 Heat Transfer Area the surface of the refrigerator, m ² 8 Heat transfer coefficient cold receiver, W / m ² ⋅ ° С 87-93 Heat Transfer Area capacitor surface, m ² 6 Heat transfer coefficient condenser, W / m ² ⋅ ° С 47-51 Refrigerant water

Example No. 1

The enzyme preparation of inulinase obtained by the in-depth method using the producer of micromycete Aspergillus awamori 2250 [Shevtsov A.A., Tertychnaya I.V., Tertychnaya T.N. The use of inulinase in the production of dietary food products // Status, problems and prospects of production and processing of agricultural products: Materials of the international. scientific-practical Conf., dedicated to the 10th anniversary of the faculty of food technology, March 29-30, 2011 - Ufa, 2011. - S. 355-357].

Inulinase is an enzyme that hydrolyzes inulin in Jerusalem artichoke, Jerusalem artichoke, dandelion and chicory to fructose. Fructose is 1.73 times sweeter than sucrose, it is less karyogenous, and in this regard is increasingly used as a sugar-containing component in diabetic nutrition.

The maximum activity of the target product was achieved in the following fermentation mode:

The composition of the nutrient medium,%:

Molasses concentration 5,0 Concentration (NH ₄ ) ₂ HPO ₄ 1,0 Concentration MgSO ₄ ⋅ 7H ₂ O 0.05 Concentration KH ₂ PO ₄ 0.1 Sterile air pressure when fed to the fermenter, MPa 0.02-0.03 Mixer rotation frequency, s ^-1 3,5-3,6 pH of the liquid phase 4.2 Fermentation temperature, ° С 31 ± 0.5 The content of CB filtrate of the culture fluid,% 7.0 ± 0.5 The activity of inulinase, units / cm ³ 25 ± 3 The activity of β-fructofuranosidase, units / cm ³ 100 ± 5 Duration of fermentation, h 96

Example No. 2

Glucoamylase Aspergillus awamori VUDT-2 was grown, which was widely used as a saccharifying enzyme in the alcohol and brewing industries [Yakovlev AN, Zherebtsov NA, Grigorav B.C., Shuvaeva GP Amylase of thermotolerant micromycete A. awamori VUDT-2 of preparative preparation // Biotechnology. - 1994. - No. 3. - S. 11-14; Yakovlev A.N., Tertychnaya T.N., Bakulin O.V. The selection of a rational nutrient medium for the biosynthesis of amylases by A. awamori VUDT-2 micromycete // Abstracts of international scientific and practical reports. Conf. "Scientific and technological progress in fermentation industries." - May 29-31, 1997 - Voronezh, 1997. - S. 39].

The enzyme allows you to fully hydrolyze starch to glucose, which is necessary for the process of fermentation and increase the yield of ethyl alcohol. The maximum activity of the target product in the culture fluid was achieved with the following fermentation mode:

The composition of the nutrient medium,%:

Starch concentration 5.3 Concentration (NH ₄ ) ₂ HPO ₄ 1,1 Concentration MgSO ₄ ⋅ 7H ₂ O 0.06 Concentration KH ₂ PO ₄ 0.14 Sterile air pressure when fed to the fermenter, MPa 0.025 ± 0.005 Mixer rotation frequency, s ^-1 3.8 ± 0.5 pH of the liquid phase 4,5 Fermentation temperature, ° С 31 ± 0.5 The content of CB filtrate of the culture fluid,% 6.0 ± 0.5 The activity of the enzyme, units / cm ³ 195 ± 5 Duration of fermentation, h 120

Thus, the proposed control method allows for the accuracy and reliability of controlling the process of biomass production of aerobic microorganisms and create conditions for the implementation of energy-efficient technology in the continuous operation of the main and auxiliary equipment.

Connecting a steam ejector chiller operating in the heat pump mode to control heat and material flows in closed thermodynamic cycles allows us to implement the proposed control method as an energy-saving and environmentally friendly biotechnology with minimal error in stabilizing abiotic factors.

Thus, the proposed method has the following advantages compared with the prototype:

- provides stabilization of parameters in the field of optimal values, ensuring the maximum yield of the finished culture due to high accuracy and reliability of control (a purely biotechnological task);

- prevents the release of waste coolants into the environment (environmental challenge);

- carries out the recovery of the heat of condensation of the exhaust vapors after the ejector in the condenser for heating water, as well as regenerative heat transfer for the preparation of cold water in the cold receiver of the steam ejector refrigeration machine (energy saving task);

- creates the conditions for reducing the cost of biomass and reduces the specific energy consumption by 5 ... 7% (the task of economic feasibility);

- allows you to use the heat of low temperature potential, in particular waste heat of gas turbine plants and boiler units (the task of rational use of heat and energy resources);

- reduces operating costs for the production of biomass of aerobic microorganisms through the use of a reliable steam ejector refrigeration machine operating in the heat pump mode (the task of saving energy costs).

Claims (1)

A method for controlling the biomass production process of aerobic microorganisms, comprising preparing a liquid seed culture in an inoculator with a cooling jacket and a stirrer; growing a microorganism culture in a fermenter with a heating jacket and stirrer; storage of the finished culture of the microorganism in the collections of the finished culture with a cooling system; preparation of cold water with a temperature of 7 ... 10 ° C and warm water with a temperature of 27 ... 47 ° C in a steam ejector chiller, consisting of an ejector, an evaporator, a cold receiver, a condenser, a thermostatic valve, a waste water collector, a steam generator with electric heating elements and a safety valve, a pump for supplying water to a steam generator, a refrigerant recirculation pump through a cold receiver, operating in a closed thermodynamic cycle; the use of a portion of the steam obtained in the steam generator for pre-sterilization of the inoculator, fermenter and collections of the finished culture; the supply of cold water to the cooling jacket of the inoculator and the cooling system of the collections of the finished culture and warm water to the heating jacket of the fermenter with the formation of recirculation circuits for material and heat flows, characterized in that they measure the temperature of preparation of the liquid seed culture in the inoculator; the temperature of the culture of the microorganism in the fermenter; steam output of a steam generator; steam consumption from the steam generator for preliminary sterilization of the inoculator, fermenter and ready-made culture collectors; optical density of the finished culture; working steam consumption in the ejector; the amount of vacuum in the evaporator and the flow rate of the ejected steam from the evaporator, the level and temperature of the refrigerant in the evaporator, the level and pressure of water in the steam generator; cold water temperature before and after the cold receiver; the flow rate of water supplied to the cooling jacket of the inoculator and the cooling system of the collections of the finished culture; temperature of the mixture of working and ejected vapors before and after the condenser; the temperature of the warm water supplied to the jacket of the fermenter; set the desired steam capacity of the steam generator at the inlet of the ejector by affecting the power of the electric heating elements of the steam generator and the ejection coefficient of the steam ejector chiller by affecting the ratio of the working and ejected steam by changing the flow rate of the ejected steam; the measured values of the temperature of the water at the inlet and outlet of the cold receiver, the temperature of the refrigerant in the cold receiver and the water flow at the inlet of the cold receiver determine the value of the heat transfer coefficient from the refrigerant to the water through the cooling surface of the cold receiver and control the temperature of the cold water by influencing the heat transfer coefficient, and when the temperature deviates of cold water supplied to the cooling jacket of the inoculator and the cooling system of the collections of the finished culture, increasing above 10 ° C dissolved heat transfer coefficient of the influence of the increase in the flow of refrigerant from the evaporator recirculation loop holodopriemnik through the evaporator, and cold water at a temperature deviation of less than 7 ° C heat transfer coefficient is reduced to reduce the influence of the coolant flow in the recirculation circuit from the evaporator to the evaporator through holodopriemnik; set the temperature of warm water supplied to the fermenter jacket from the condenser by affecting the ejection coefficient by changing the flow rate of the working steam at the inlet of the ejector with a correction for the temperature of growing the microorganism culture in the fermenter; and stabilize the concentration of the finished culture by the optical density of the finished culture by affecting the frequency of rotation of the stirrer of the fermenter.