RU2540467C1 - Sulfogel-preparation for treatment of infected wounds in toe region of animals and method of its use - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: preparation for treatment of infected wounds in the toe region of animals, containing dimethyl sulphoxide, methyl cellulose, additionally comprises tylosin tartrate, sulphadimine, trimethoprim in the following ratio of components, wt %: tylosin tartrate - 2-3, sulphadimine - 4-5, trimethoprim - 1-1.5, dimethyl sulphoxide - 10-15, methylcellulose - 3.0-3.5, water - the rest. The claimed method comprises applying the claimed preparation without preliminary wound cleaning. The preparation is applied to the wound with the layer thickness of 2-3 mm, without application of soft dressing. The treated animals are kept for 2-3 hours on the dry surface.

EFFECT: use of claimed group of inventions increases the efficiency of treatment.

4 cl, 7 tbl, 2 ex

Description

The invention relates to the field of veterinary medicine, in particular to drugs used to treat purulent necrotic wounds, ulcers, phlegmon, subdermatitis and other pathologies in the fingers of cattle or sheep caused by the multiplication in wounds of various microorganisms, including the causative agent of necrobacteriosis.

Known drug against necrobacillosis of cattle and respiratory diseases of calves, a method for the treatment and prevention of necrobacillosis of cattle and respiratory diseases of calves (RF patent No. 2262936 from 20.20.2005, IPC A61K 31/70, A61P 11/00, A61P 31 / 00), in which tylosin tartrate, gelatin, lanolin is used by intramuscular injection into a massive muscle group, three times, with an interval of 2-3 days.

The disadvantage of this method is that the drug is administered intramuscularly, the products of which are excreted in milk and stored for a long time in meat, which provides for the rejection of milk during treatment, limiting the use of meat after slaughter for 8 days after the last injection.

The closest technical solution, selected as a prototype, is the drug Necrogel (RF patent No. 2204997, IPC АКК 33/00, 33/34, dated May 27, 2003).

However, the ingredients used in this preparation cause irritation, burning and pain, toxic, poorly tolerated.

The task of the invention is to expand the arsenal of tools for treating infected wounds in the fingers of animals, including the causative agent of necrobacteriosis.

The problem is solved in that the preparation for the treatment of wounds in the area of the fingers of animals, containing dimethyl sulfoxide and methicellulose, according to the invention additionally contains tylosin tartrate, sulfadimesin, trimethoprim in the following ratio, wt.%:

Tylosin tartrate  2-3 Sulfadimezin  4-5 Trimethoprim  1-1.5 Dimethyl sulfoxide  10-15 Cellulose  3.0-3.5 Water  Rest

The problem is also solved by the fact that in the method of using the drug for the treatment of animals infected in the fingers, the drug is applied to the wound without a prior toilet.

The problem is also solved by the fact that the drug is applied with a layer thickness of 2-3 mm.

The problem is also solved by the fact that the animals after treatment are put on a dry surface for 2-3 hours.

The drug was prepared as follows.

For one kilogram of the preparation, 740 ml of distilled water was added to the enameled container and 30 g of methylcellulose was gradually added with constant stirring and left for 23-24 hours to completely dissolve the methylcellulose, periodically stirring and rubbing the lumps. Then, 30 g of tylosin tartrate, 50 g of sulfadimesin, 15 g of trimethoprim and 150 ml of dimethyl sulfoxide were alternately introduced into the methylcellulose solution with constant stirring. After 2-3 hours, the ointment was again thoroughly mixed and packaged in a container (white plastic jars with a capacity of 0.5 l). If you need a larger amount of the drug, these quantities increase in multiples of the need.

To determine the effective formulation, 4 variants of the preparation were prepared according to the composition (Table 1)

Table 1 Component Recipe 1 Recipe 2 Recipe 3 Recipe 4 Tylosin tartrate one 2 3 four Sulfadimezin 3 four 5 6 Trimethoprim 0.5 1,0 1,5 2 Cellulose 2,5 3.0 3,5 4.0 Dimethyl sulfoxide 3 5 10 fifteen Water 89.5 85 73.5 70

To determine the therapeutic efficacy of each prescription, 4 groups of 6 animals with wounds in the fingers were selected. The preparation of the test variant of the formulation was applied to the infected wound and the animal was placed on a dry surface for 2-3 hours. Every 3-4 days, this procedure was repeated until the outcome of the disease (recovery or worsening). The therapeutic effect was evaluated by the number of recovered animals. The results of the therapeutic effect of the drug options are given in table.2.

table 2 Recipe Option Processed animals Recover, goal. Efficiency, treatment,% one 6 3 fifty, 2 6 5 83.3 3 6 6 100.0 four 6 6 100.0

From the results shown in table 2, it is seen that all the experimental animals recovered with the formulation according to option 3 and 4. Since the achieved effect was already with the formulation according to option 3, it was chosen for the subsequent study as effective and less expensive. This drug is given the working name Sulfogel.

Next, the minimum inhibitory concentration (MIC) of the Sulfogel preparation was determined in comparison with the control Necrogel preparation with respect to microorganisms involved in infection of wounds in the finger region, both to reference strains and to wound isolates (Table 3).

Table 3 Test microbe MIC, mg / ml Sulfogel Necrogel Fusobacterium necrophorum strain "IV" 0.7 1.3 Fusobacterium necrophorum (isolate) 0.7 1,1 Escherichia coli ATS 25922 5.3 7.7 Escherichia coli (isolate) 5,4 7.9 Staphilococcus aureus ATCC 25923 3.8 4.3 Staphylococcus aureus (isolate) 4.1 4,5 Streptococcus (isolate) 3.8 4.3 Micrococcus (isolate) 20.1 23,4

As can be seen from table 3, sulfogel had an inhibitory effect at lower concentrations than necrogel.

Physical properties (color of the drug) of Sulfogel and Necrogel during storage are shown in table 4.

Table 4 A drug Original color Color during storage (month) 3 6 9 12 Sulfogel White White White White Light yellow Necrogel Light yellow Light brown Brown Dark brown Brown

As can be seen from table 4, a significant change in the color of the Necrogel occurs after 6 months of storage, namely after 9 and 12 months. In this connection, in the following experiments, the therapeutic efficacy of Sulfogel and Necrogel was tested after different storage times. The results of medical work are shown in table 5.

Table 5 The drug and storage time (mc) Processed animals Recover, goal. Efficiency% Duration of treatment, days Sulfogel - 9 6 6 100.0 12-16 Sulfogel - 12 6 6 100.00 16-20 Necrogel - 9 6 3 50,0 20-24 Necrogel - 12 6 2 33.3 24-28

Thus, from the presented data on physical properties, inhibitory concentration and therapeutic efficacy, we can conclude that the proposed drug Sulfogel has significantly better performance than the drug Necrogel.

In the claimed invention, tylosin tartrate, an antibiotic from the macrolide group, was used as active against most gram-positive and some gram-negative bacteria, including Fusobacterim spp., Staphylococcus spp., Streptococcus spp., Corynebacterium spp., Clostridium spp., Involved in inflammation. Tylosin is a low toxic compound. Tylosin is not used to treat people - it is an antibiotic exclusively for veterinary use, so the risk of developing cross-resistance of human bacterial pathogens is extremely small. The drug, unlike other antibiotics, has pronounced immunomodulating and immunostimulating properties, which makes its use more effective.

The well-known sulfanilamide preparation sulfadimesin in combination with trimethaprim has a wide spectrum of antibacterial activity and has a bactericidal effect, in contrast to other sulfonamides (sulfadimethoxin), which act bacteriostatically. The combination of these drugs, each of which has a bacteriostatic effect, creates a cumulative effect, provides high bactericidal activity against gram-positive and gram-negative microorganisms, including bacteria resistant to sulfonamide drugs.

The invention is illustrated by the following examples.

Example 1. Evaluation of the therapeutic effectiveness of the drug in a farm.

In the experiment there were 2 groups of 15 animals each (experimental and control) selected by the method of renomination — the first animal to the experimental group, the second to the control and so on until the groups were completely formed. In the experimental group, Sulfogel was applied to the wound; in the control group, Necrogel with a shelf life of not more than 3 months. After applying Sulfogel, the animals were kept for 2-3 hours on a dry surface. In the control group, Necrogel was applied after the toilet (cleaning) the wound and a soft bandage was applied. Repeated treatments in both groups were performed after 4 days. The effectiveness of the treatment was evaluated according to the following parameters: the duration of treatment, the outcome of the disease, the percentage of recovery (Table 6).

Table 6 Group / Drug Processed animals Recover, goal. Efficiency% Duration of treatment, days Experienced / Sulfogel fifteen fifteen 100.0 12-16 Control / Necrogel fifteen 13 86.70 16-20

From the results shown in table 6, it is seen that treatment with Sulfogel had an advantage in terms of effectiveness (100%) and duration of treatment (12-16 days).

Example 2. Determination of the therapeutic efficacy of Sulfogel without prior toilet wounds (table 7).

Table 7 Treatment option Animals Recover, goal. Efficiency% Duration of treatment, days Without toilet wounds, 1-2 mm layer 6 four 66.70 20-24 Without toilet wounds, 2-3 mm layer 6 6 100.00 16-20 Without toilet wounds, 3-4 mm layer 6 6 100.00 16-20 With a toilet, a layer of 2-3 mm soft dressing 6 6 100.00 16-20

The data given in Table 7 show that the effectiveness of treatment without a wound toilet was when applying it with a layer of 2-3 mm and a subsequent increase in the layer thickness. Therefore, it is advisable to use Sulfogel with a layer of 2-3 mm as an effective and low-cost.

Thus, the drug Sulfogel for the treatment of infected wounds in the fingers of animals has high therapeutic efficacy (100%), easy to prepare and use. The use of Sulfogel in veterinary practice helps to preserve the livestock of animals, prevent economic damage from finger diseases, including necrobacteriosis.

Claims (4)

1. The drug for the treatment of infected wounds in the fingers of animals containing dimethyl sulfoxide, methyl cellulose, characterized in that the preparation additionally contains tylosin tartrate, sulfadimesin, trimethoprim in the following ratio, wt.%:
Tylosin tartrate 2-3 Sulfadimezin 4-5 Trimethoprim 1-1.5 Dimethyl sulfoxide 10-15 Cellulose 3.0-3.5 Water Rest 2. The method of application of the drug for the treatment of infected wounds in the fingers of animals, characterized in that the drug according to claim 1 is applied without prior toilet wounds. 3. The method according to p. 2, characterized in that the preparation is applied to the wound with a layer of a thickness of 2-3 mm, without applying a soft dressing. 4. The method according to p. 2, characterized in that the treated animals can withstand 2-3 hours on a dry surface.