RU2371185C1 - Method of fibrin powder obtainment - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention concerns medicine, particularly hematology and obtainment of medicines out of animal and human blood. Invention claims simple method of fibrin powder obtainment, involving fibrin separation from blood clot, flushing by distilled water, drying by filter paper till complete water evaporation, and further grinding and sterilisation of target product. After drying by filter paper separated fibrin is placed for 2-4 hours to 9-10% ammonium solution in amount of 1 g of raw fibrin per 4-6 ml of solution, and then it is dried at 30-45°C till complete evaporation of water and ammonium.

EFFECT: simplified procedure, obtainment of sterile hygroscopic fibrin powder.

Description

The invention relates to medicine, in particular to hematology, and relates to the search for new possibilities for obtaining and improving medical preparations from human and animal blood.

A known method for producing fibrin powder consists of isolating it from a blood clot of animals or humans, followed by drying at a temperature of 35-40 ° C for 20-24 hours and sterilization at a temperature of 120 ° C at atmospheric pressure of 2.5 atm for 30 minutes ( one).

However, this method has the following disadvantage. Fibrin isolated from a blood clot, when dried, loses water and turns into very dense lumps, which require a long time to grind them.

The purpose of the invention is to obtain a sterile powder from human and animal blood fibrin (domestic pig, horse), convenient for use in treatment, to simplify the technology of its production with shortening the time of release of the final product.

The goal is achieved as follows.

After blood sampling from donors or animals in a container and the formation of blood clots in them, fibrin is released from them by a known method (2). After isolation, fibrin is washed with distilled water, dried with filter paper and placed in a 9-10% ammonia solution at the rate of 1 g of crude fibrin in 4-6 ml of solution for 2-4 hours, and then poured onto a dry, flat surface and dried at a temperature of 30- 45 ° C until complete evaporation of water with ammonia. After this, fibrin is removed from the surface, ground in the usual way and sterilized at a temperature of 120 ° C at atmospheric pressure of 2.5 atm for 30 minutes.

End product characterization

Obtained by the proposed method, fibrin powder, regardless of donor (human, domestic pig, horse), has a brownish color, when dissolved in water, it gives a colloidal opalescent solution. When combined with water, it swells, and when placing its aqueous solution on a dry, even surface (1 g of dry powder in 2.0 ml of distilled water) and drying for 20-24 hours at a temperature of 35-40 ° C, it turns into a dense fibrin crust.

Comparative evaluation of the final product obtained by the prototype and the proposed method.

To evaluate the final product - fibrin powder, we selected for comparison the time of its grinding in the same mortar by the same person, the possibility of adsorption of water and the percentage of insoluble sediment when it is combined with water.

For grinding, fibrin isolated from pig blood was taken in an amount of 1 g on a dry matter basis. As these studies showed, for grinding 1 g of dry fibrin according to the prototype method, it takes 10-12 minutes, and 7-9 minutes according to the proposed method. In addition, 1 g of crushed fibrin, obtained by the prototype, adsorbs 1.0-1.2 ml of water, according to the proposed method, 1 g of crushed fibrin adsorbs 1.3-1.5 ml of water, which indicates its finer structure. When dissolved in water, an insoluble precipitate remains, which after filtering in the prototype is 32-35%, and in the obtained by the proposed method is 23-25%.

Using the present invention allows to obtain fibrin powder in a faster way to obtain a better final product.

Literature

1. Patent No. 1811845 A1. A method of producing fibrin powder.

Authors V.A.Figurnov, E.V.Figurnova.

2. Patent No. 1573571 A1. A method of obtaining fibrin from a blood clot.

Authors V.A.Figurnov, A.V.Figurnov.

Claims (1)

A method of producing fibrin powder, including the isolation of fibrin from a blood clot, washing it with distilled water, drying with filter paper, drying to completely evaporate the water, followed by grinding and sterilization of the target product, characterized in that the selected fibrin after drying with filter paper is placed for 2-4 hours in a 9-10% ammonia solution at the rate of 1 g of crude fibrin per 4-6 ml of solution, and then dried at a temperature of 30-45 ° C until complete evaporation of water and ammonia.