RU2352344C2 - Method of cows endometritis prevention - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: method consists that microbial preparation is probiotic Sakhabactisubtil representing suspended strains of bacteria Bacillus subtilis TNP-3-DEP and Bacillus subtilis TNP-3-DEP half-and-half. Probiotic Sakhabactisubtil is introduced in dosage 30 ml within 10 days of every month, three months prior to calving.

EFFECT: method normalises vaginal and intestinal microbiocoenosis, improves immunobiological reactivity, prevents endometritis and ensures a healthy animal yield.

Description

The invention relates to the field of veterinary medicine, in particular to the prevention of postpartum complications of cows.

The following analogues are known for the prevention of endometritis treatment:

- Probiotic sporobacterin (Collection of proceedings of the Orenburg State Agrarian University, Orenburg, 2000, p. 47);

- probiotic zoo-feed (Veterinary of farm animals, 2005, No. 11, p. 54-55);

- probiotic spormetrin (Veterinary of farm animals, 2005, No. 8, p. 43-44. J. Veterinary, 2002, No. 11, p. 28-29).

They used probiotic strains of you.subtilis No. 15, Lactobacillus planfarum, Bifidobacferium bifidum, Bacillus subtilis 534, which were administered intrauterine, with food and intramuscularly.

However, there is no information on the use of the probiotic Sahabactisubtil from the bacterial strains Bacillus subtilis TNP-3-DEPT and Bacillus subtilis TNP-5-DEPT for the prevention of cow endometritis.

The bacterial strains "Bacillus subtilis TNP-3-DEPT" and "Bacillus subtilis TNP-5-DEPT" are used to disinfect bird droppings and manure from pathogenic microorganisms (RF patent for the invention No. 2105810, C12N 1/20, published. 02.27.1999 g ., bull. No. 6), for the prevention of diarrheal diseases of newborn calves and piglets (RF patent No. 22118924, A61P 1/100, published. 12/20/2003, bull. No. 35).

The technical result of using the probiotic Sahabactisubtil from the bacterial strains Bacillus subtilis TNP-3-DEPT and Bacillus subtilis TNP-5-DEPT is to suppress the development of pathogenic and conditionally pathogenic microorganisms in the genitals, normalize vaginal and intestinal microbiocenosis, increase immunobiological reactivity.

The technical result of the claimed invention is achieved as follows: the probiotic "Sahabactisubtil" from the bacterial strains "Bacillus subtilis TNP-3-DEPT" and "Bacillus subtilis TNP-5-DEPT" is used for pregnant cows 3 months before calving within 10 days of each month. The drug has a wide spectrum of antagonistic activity against pathogenic and conditionally pathogenic microorganisms. Prevents dysbiosis of the genital and intestinal tract and increases the body's immunobiological reactivity.

The probiotic “Sahabactisubtil” is made from the bacterial strains “Bacillus subtilis TNP-3-DEP” and “Bacillus subtilis TNP-5-DEP” deposited in the collection of microorganisms of the All-Russian State Institute of Control, Standardization and Certification of Veterinary Medicines (VGNKI). The certificates of deposit were issued on October 17, 1994 for No. 902/9 and on February 6, 2001 for No. 180/15 and No. 181/15). The bacterial strains "Bacillus subtilis TNP-3-DEPT" and "Bacillus subtilis TNP-5-DEPT" are characterized by antagonistic activity against pathogenic microorganisms and the ability to stimulate the immunobiological reactivity of the body.

Strains can be obtained in the laboratory for the development of microbial preparations of GNU Yakutsk Research Institute of Agriculture SB RASHN and VGNKI.

The manufacture, control and testing of the drug “Sahabactisubtil” is carried out according to the technical conditions “The drug Sahabactisubtil for the prevention and treatment of diarrhea of newborn calves” No. 9383-003-00670203-01 of March 12, 2001

The bacterial strains Bacillus subtilis TNP-3-DEPT and Bacillus subtilis TNP-5-DEPT are grown in meat-pentone agar pH 7.0-7.2 for 5 days at 37 ° C, then suspended in different proportions in physiological saline sodium chloride at a concentration of 5 billion microbial cells in 1 ml. To prepare a single dose, 150 billion microbial cells (30 ml of suspension) are mixed with 20 g of feed.

To prevent postpartum complications of cows, 47 heads of pregnant cows were selected 3 months before calving. The first group (25 animals) is given the Sahabactisubtil preparation containing 150 billion microbial cells in 30 ml with feed for 10 days each month. The drug is used 3 months in a row before calving. The control group (22 goals) does not receive the drug.

During the observation period (2 months), among the cows treated with the probiotic Sahabaktisubtil, 1 head (4%) fell ill with endometritis. The service period was 67.8 days. In the control group, three cases of the disease were recorded (13.6%), the service period was 76.2 days. In the blood of cows treated with the probiotic "Sahabactisubtil", the number of leukocytes and hemoglobin, the bactericidal and lysozyme activity of blood serum increase compared with the control group.

In the study after calving of vaginal-cervical mucus of cows that did not take the drug, the phenomenon of dysbiosis was observed: the absence of lactobacilli and bifidobacteria, the presence of toxigenic fungi like Asp.niger, Asp.fumigatus, Asp.flavus, Asp.nidulans, pathogenic staphylococci (600.0 ± 12.0 thousand square meters), an increase in the number of Escherichia by 1.31 times (14.02 ± 0.67 thousand square meters), enterococci by 3.92 times (3.65 ± 0.33 thousand square meters). The most optimal microbiocenosis was registered in cows taking the drug: the absence of the above toxigenic fungi was noted, except for Candida paraplilosus, the number of spore-forming bacteria increased by 2.28 times (19.37 thousand m.k.), bifidobacteria and lactic acid bacteria appeared ( 1.6 thousand square meters), no staphylococci.

In the cows of the experimental group, when the probiotic “Sahabactisubtil” is orally administered, representatives of the normoflora noticeably increase after the experiment in the intestinal microbiocenosis: the number of lactobacilli is 3 thousand times greater (2.59 ± 0.005 thousand square meters), bifidobacteria are also more - 2 orders of magnitude, than in control. The number of spore-forming bacteria also increases by 4 times (35.33 ± 2.15 thousand square meters), lactose-positive Escherichia by 6 times (63.51 ± 0.26 thousand square meters) than in the control. Toxigenic fungi are less in the experimental group. It should be noted a significant decrease (1.5 times) of potential entropathogens - lactose-negative Escherichia (9.20 ± 0.12 thousand square meters)

The results of a study of the intestinal microflora of newborn calves obtained from cows that had taken the Sahabactisubtil drug orally before calving showed that in the calves of the experimental group, the content of lactobacilli was 101.35 times higher (1875 ± 4.71 thousand sq.m.) bifidobacteria and spore-forming bacteria are 9.83 times (49.16 ± 0.38 thousand square meters) than in calves of the control group. A decrease in the number of Escherichia by 28.57 times (150 ± 5 thousand square meters), enterococci by 18.24 times (1750 ± 6.5 thousand square meters), toxigenic fungi in calves of the experimental group was noted. There were also no signs of gastrointestinal upsets in calves obtained from cows that received the probiotic Sahabactisubtil before calving. In newborn calves obtained from cows of the control group, diarrhea was observed.

Thus, oral administration of the probiotic “Sahabactisubtil” with compound feed prior to calving increases the immunobiological reactivity of the body of pregnant cows, prevents dysbiosis of the vaginal and intestinal tracts, thereby contributing to the prevention of entometritis and the production of healthy calves.

Claims (1)

A method for the prevention of cow endometritis by administering the microbial preparation inward, characterized in that the probiotic Sahabactisubtil, which is a suspension of the bacterial strains Bacillus subtilis TNP-3-DEPT and Bacillus subtilis TNP-5-DEPT, is used as a microbial preparation. ratios in a dose of 30 ml for 10 days of each month three months before calving.