RU2270806C2 - Strain pseudomonas aeruginos xp-25 carrying out biodegradation of aromatic compounds - Google Patents
Strain pseudomonas aeruginos xp-25 carrying out biodegradation of aromatic compounds Download PDFInfo
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- 150000001491 aromatic compounds Chemical class 0.000 title claims abstract description 12
- 238000006065 biodegradation reaction Methods 0.000 title claims description 5
- 241000589516 Pseudomonas Species 0.000 title 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 10
- 230000001580 bacterial effect Effects 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 239000002689 soil Substances 0.000 abstract description 4
- 230000015556 catabolic process Effects 0.000 abstract description 2
- 238000006731 degradation reaction Methods 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 239000010865 sewage Substances 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 15
- 239000000203 mixture Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 240000007313 Tilia cordata Species 0.000 description 1
- MWPLVEDNUUSJAV-UHFFFAOYSA-N anthracene Chemical compound C1=CC=CC2=CC3=CC=CC=C3C=C21 MWPLVEDNUUSJAV-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 239000010840 domestic wastewater Substances 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
Description
Изобретение относится к биотехнологии и касается нового штамма бактерий, который может быть использован для очистки сточных вод химических предприятий, содержащих ароматические соединения.The invention relates to biotechnology and relates to a new strain of bacteria that can be used for wastewater treatment of chemical plants containing aromatic compounds.
Наиболее близким по сущности является штамм Pseudomonas aeruginosa №28, разлагающий фенол [АС СССР №499227 по заявке №203990 от 01.07.74]. Однако известный штамм разлагает фенол лишь в концентрации 200-300 мг/л при продолжительности 24 часа до 99,6%, смесь летучих и нелетучих фенолов при суммарной концентрации 200 мг/л в течение 48 часов.The closest in essence is the strain Pseudomonas aeruginosa No. 28, decomposing phenol [USSR AS No. 499227 by application No. 203990 from 01.07.74]. However, the known strain decomposes phenol only at a concentration of 200-300 mg / L with a duration of 24 hours to 99.6%, a mixture of volatile and non-volatile phenols at a total concentration of 200 mg / L for 48 hours.
Задачей изобретения является получение нового штамма, с высокой эффективностью разлагающего ароматические соединения, в частности, фенол и другие ароматические соединения, в сточных водах нефтехимических производств.The objective of the invention is to obtain a new strain, with high efficiency decomposes aromatic compounds, in particular phenol and other aromatic compounds, in wastewater of petrochemical plants.
Поставленная задача реализуется выявлением и использованием штамма Pseudomonas aeruginosa для биодеградации ароматических соединений. Штамм Pseudomonas aeruginosa XP-25 был выделен из фенолзагрязненых почв Стерлитамакского нефтехимического завода путем культивирования почвенного образца в минерализованной среде и последующим отбором наиболее активных форм. Штамм депонирован во Всероссийской коллекции промышленных микроорганизмов под номером В-8613, осуществляет эффективную биологическую деградацию ароматических соединений и отличается от известных морфологическими, физиологическими и культуральными признаками.The task is realized by the identification and use of the strain Pseudomonas aeruginosa for biodegradation of aromatic compounds. The strain Pseudomonas aeruginosa XP-25 was isolated from phenol-contaminated soils of the Sterlitamak Petrochemical Plant by cultivating the soil sample in a mineralized medium and then selecting the most active forms. The strain is deposited in the All-Russian collection of industrial microorganisms under the number B-8613, carries out effective biological degradation of aromatic compounds and differs from the known morphological, physiological and cultural characteristics.
Морфологические признаки. Палочки с 1 полярным жгутиком. Грамотрицательны. Спор не образуют. В мазках располагаются поодиночке, парами, короткими цепочками.Morphological signs. Sticks with 1 polar flagellum. Gram-negative. They do not form a dispute. In strokes are located singly, in pairs, in short chains.
Культуральные признаки. Рост на мясо-пептонном агаре колониями серовато-белого цвета с неровными краями. Отмечали синевато-зеленое окрашивание среды и характерный запах цветущей липы. На мясо-пептонном бульоне через 18 часов отмечалось равномерное помутнение среды, появление толстой пленки на поверхности и осадка на дне. Характерно образование слизи.Cultural signs. Growth on meat-peptone agar in grayish-white colonies with uneven edges. A bluish-green staining of the medium and a characteristic smell of flowering linden were noted. After 18 hours, a uniform turbidity of the medium, the appearance of a thick film on the surface and sediment at the bottom were observed on the meat-peptone broth. The formation of mucus is characteristic.
Физиологические признаки. Аэроб, оптимальная температура роста 25-33°С, максимальная 42°С, отсутствие роста при 4°С.Physiological signs. Aerob, optimal growth temperature 25-33 ° C, maximum 42 ° C, no growth at 4 ° C.
Биохимические признаки. Гидролизует желатину, крахмал не гидролизует. Растет в чисто минеральной среде, в факторах роста не передается. Утилизирует цитрат на среде Симменса, оксидаза положительна. Расщепляет глюкозу в среде Хью-Лейфсона в аэробных условиях, в анаэробных условиях лактозу не расщепляет, не образует индол.Biochemical signs. Hydrolyzes gelatin, starch does not hydrolyze. It grows in a purely mineral environment, is not transmitted in growth factors. It utilizes citrate on Simmens' medium; oxidase is positive. It breaks down glucose in a Hugh-Leifson medium under aerobic conditions; under anaerobic conditions, it does not break down lactose and does not form indole.
При ферментации на качалочных колбах штамм разрушает фенол в концентрации 1000 мг/л за 4 часа на 99,98%. На проточной лабораторной установке в иммобилизованном состоянии на активированном угле штамм окисляет фенол концентрацией не менее 2500 мг/л за 1 час.When fermented on rocking flasks, the strain destroys phenol at a concentration of 1000 mg / l in 4 hours by 99.98%. In a flowing laboratory setup in an immobilized state on activated carbon, the strain oxidizes phenol with a concentration of at least 2500 mg / l in 1 hour.
Культивирование в лабораторных условиях. Фенолразрушающую активность штамма Pseudomonas aeruginosa XP-25 поддерживают на жидкой минерализованной среде, в которую добавлен фенол, например, следующего состава, г/л:Cultivation in the laboratory. The phenol-destructive activity of the strain Pseudomonas aeruginosa XP-25 is maintained on a liquid mineralized medium into which phenol is added, for example, of the following composition, g / l:
pH 7,2.pH 7.2.
Суспензию бактерий вводят в жидкую среду путем смыва с косячков небольшим количеством стерильной водопроводной воды из расчета 1-3 об.%.A suspension of bacteria is introduced into the liquid medium by rinsing from the jamb with a small amount of sterile tap water at a rate of 1-3 vol.%.
Штамм хранится на твердых агаровых средах при температуре +4°С.The strain is stored on solid agar media at a temperature of + 4 ° C.
Приведем примеры использования штамма Pseudomonas aeruginosa ХР-25 для биодеградации ароматических соединений.Here are examples of the use of the strain Pseudomonas aeruginosa XP-25 for biodegradation of aromatic compounds.
Пример 1.Example 1
В минерализованную среду вышеприведенного состава с инкубированным штаммом вносится фенол в концентрации 500 мг/л. После часовой инкубации в колбах на качалке (200 об/мин, Т=34°С) остаточная концентрация фенола 150 мг/л, через 2 часа фенола не обнаруживается.Phenol at a concentration of 500 mg / l is introduced into the mineralized medium of the above composition with an incubated strain. After an hour-long incubation in flasks on a shaker (200 rpm, T = 34 ° C), the residual phenol concentration is 150 mg / l; after 2 hours phenol is not detected.
Пример 2.Example 2
В среду вносится фенол в концентрации 1000 мг/л. Через 3 часа инкубации - 87,3% утилизации, через 4 часа полное отсутствие.Phenol is added to the medium at a concentration of 1000 mg / L. After 3 hours of incubation, 87.3% of utilization, after 4 hours, complete absence.
Пример 3. В лабораторную установку, состоящую из колонки, наполненной углем с иммобилизованной биомассой, с соотношением диаметра и высоты колонки 1:10, работающую в условиях протока модельных сточных вод, подают сточную воду следующего состава, г/л:Example 3. In a laboratory setup, consisting of a column filled with coal with immobilized biomass, with a ratio of the diameter and height of the column 1:10, operating in a flow of model wastewater, wastewater of the following composition is supplied, g / l:
После часового пребывания в установке наблюдается полное отсутствие фенола на выходе.After an hour's stay in the installation, there is a complete absence of phenol at the outlet.
Примеры биодеградирующей активности штамма Pseudomonas aeruginosa XP-25 для других ароматических соединений приведены в таблице 1.Examples of biodegradable activity of the strain Pseudomonas aeruginosa XP-25 for other aromatic compounds are shown in table 1.
Примеры биодеградирующей активности штамма Pseudomonas aeruginosa XP-25 в модельной проточной установкеTable 1
Examples of biodegradable activity of the strain Pseudomonas aeruginosa XP-25 in a model flow-through installation
Во всех примерах определение содержания органических соединений осуществляют спектрофотометрически в УФ-области (240-400 нм) на приборе «Specord UV-VIS», при этом для каждого вещества строят калибровочный график.In all examples, the determination of the content of organic compounds is carried out spectrophotometrically in the UV region (240-400 nm) on a Specord UV-VIS instrument, and a calibration graph is constructed for each substance.
Таким образом, штамм Pseudomonas aeruginosa XP-25 с высокой эффективностью обеспечивает биодеградацию ароматических соединений, в частности фенола. Очищенные с помощью штамма сточные воды могут быть использованы для дальнейшей очистки с бытовыми сточными водами.Thus, the strain Pseudomonas aeruginosa XP-25 with high efficiency provides biodegradation of aromatic compounds, in particular phenol. Sewage treated with a strain can be used for further treatment with domestic wastewater.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2489484C1 (en) * | 2012-08-14 | 2013-08-10 | Федеральное государственное унитарное предприятие "Всероссийский нефтяной научно-исследовательский геологоразведочный институт" (ФГУП "ВНИГРИ") | STRAIN Pseudomonas citronellolis, USED FOR DECOMPOSITION OF OIL AND DIESEL FUEL |
| RU2601155C2 (en) * | 2012-05-31 | 2016-10-27 | Университет Варшавски | Composition of bacterial strains, mixture for bioremediation and using said composition for removal of contaminants from soil, as well as method of soil cleaning from contaminants |
| CN106754514A (en) * | 2016-12-23 | 2017-05-31 | 钟华 | A kind of sustainable desilting is except the microbial bacterial agent preparation method of black and odorous water |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3049669C (en) * | 2013-04-25 | 2021-03-30 | Sandvik Intellectual Property Ab | Gyratory crusher topshell |
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2004
- 2004-04-07 RU RU2004110626/13A patent/RU2270806C2/en active
Non-Patent Citations (1)
| Title |
|---|
| Т.В.КОРОНЕЛЛИ и др. Химический состав и роль пептидогликолипида Pseudomonas aeruginosa в процессе усвоения углеводородов. Микробиология, т.52, вып.5, 1983, с.767-769. * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2601155C2 (en) * | 2012-05-31 | 2016-10-27 | Университет Варшавски | Composition of bacterial strains, mixture for bioremediation and using said composition for removal of contaminants from soil, as well as method of soil cleaning from contaminants |
| RU2489484C1 (en) * | 2012-08-14 | 2013-08-10 | Федеральное государственное унитарное предприятие "Всероссийский нефтяной научно-исследовательский геологоразведочный институт" (ФГУП "ВНИГРИ") | STRAIN Pseudomonas citronellolis, USED FOR DECOMPOSITION OF OIL AND DIESEL FUEL |
| CN106754514A (en) * | 2016-12-23 | 2017-05-31 | 钟华 | A kind of sustainable desilting is except the microbial bacterial agent preparation method of black and odorous water |
| CN106754514B (en) * | 2016-12-23 | 2019-08-13 | 钟华 | A kind of sustainable dredging removes the microbial bacterial agent preparation method of black and odorous water |
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| RU2004110626A (en) | 2005-09-20 |
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