RU2095052C1 - Gargling composition for prophylaxis of parodont diseases - Google Patents

Abstract

FIELD: stomatology. SUBSTANCE: proposed gargling composition is intended for hygiene of oral cavity and for prophylaxis of parodont diseases, and comprises enzymatic complex consisting of cultured ginseng cells (strains IFPG-1, IFPG-5, BCKKKBP N26) and Polysciasis dryopteris plant cells (strain BCKKKBPN24). Composition is in form of aqueous and alcoholic solution containing 3.0-10.0% of said enzymatic complex. Good results were obtained by using composition. After using it for 20-22 days odontorrhagia stopped in 98% of persons under test, their painful sensations disappeared. Hygiene index before using composition was 2.69+-0.27, and it became 1.65+- 0.28 after using it. PMA index (%) was 32.6+-1.8 before and 7.9+-0.8 after using composition. EFFECT: effective new means for preventing parodont diseases. 5 cl

Description

 The invention relates to medicine, in particular to dentistry, and relates to prophylactic agents for periodontal diseases.

 Recently, among all diseases in the field of dentistry, the percentage of periodontal diseases has increased significantly. Given that these diseases are dangerous not only for the teeth, but also for the whole organism, the importance of preventing such diseases is understandable.

 For the treatment of periodontal diseases using various herbal medicines: Kalanchoe juice, plantain juice, etc. However, their effectiveness is not high enough.

For the prevention of periodontal diseases, it is known to use formulations containing biologically active substances from various plant materials:
with plantain extract and mineral and vitamin concentrate from kelp;
with senna lipid complex;
with natural coniferous complex;
with copper derivatives of chlorophyll.

 All these tools are not effective enough, multicomponents, therefore, individual intolerance is possible.

It is known to use the superoxide dismutase enzyme (SOD) for the treatment and prevention of gum disease. The proposed compositions contain SOD (human) in an amount of 1 • 10 ^-4 1 wt. may additionally contain catalase from 1 • 10 ^-4 to 1 wt. The disadvantage of these formulations is that human SOD is an expensive and unsafe drug because of the danger of infection with the immunodeficiency virus and hepatitis.

 The objective of the invention is to diversify an effective, affordable, easy-to-use composition for the prevention of periodontal diseases, to expand the range of tools available for this purpose.

 The task is carried out by the proposed composition, containing as an biologically active component an enzyme complex obtained from the biomass of cultivated plant cells of ginseng of strains IFRZH-1, IFRZH-5, VSKKKVR N 26 and polyscias of the fern-leaf strain VSKKKVR N 24. Moreover, the complex content in the composition is from 3, 0 to 10.0 wt. The composition is a water-alcohol or aqueous solution.

The composition of the enzyme complex:
SOD 20.0 ± 1.8 conventional units D ₄₆₀ / mg protein
Catalase 4.2 ± 0.6 mm H ₂ O ₂ / min, mg protein
Peroxidase 0.6 ± 0.03 conventional units D ₄₃₅ / mg protein
The complex also contains 5 ± 1.0 mg% of divalent calcium and 1.4 ± 0.2 mg / ml of the phospholipid fraction.

 The complex is not immunotoxic, has a high anti-inflammatory activity. It contains the optimal "natural" ratio of enzymes, which makes it very effective, it combines anti-inflammatory, anti-allergic, bactericidal properties. Its antimicrobial effect was tested on standard bacterial test cultures: Staphylococcus aureus, Escherichia coli, Candida genus fungi. Therefore, effective formulations comprising this complex may contain a minimal set of components. To create a pleasant taste can be introduced food flavorings in an amount of 0.2 to 0.5 wt. to create a pleasant odor of fragrance 0.05 0.1% citric acid to create a favorable pH, xylitol (sorbitol) food to remove plaque.

 The enzyme complex (FC) is used to prepare formulations in the form of lyophilized powder.

The technology for preparing the composition consists of the following operations:
the dissolution of perfumes (menthol or peppermint oil) in ethanol in a ratio of 1 to 3,
dissolution of xylitol (sorbitol) in water in a ratio of 1 to 5,
dissolution of citric acid in water in a ratio of 1 to 4,
the combination of prepared solutions with the remains of water and alcohol according to the recipe,
FC dissolution
exposure 5 6 hours
filtration.

 All operations are carried out at room temperature.

 Example 1

FC from biomass pcs. IFRZH-1 3.0 g
Ethyl alcohol 30.0 g
Menthol 1.1 g
Food flavoring 0.3 g
Water dist. Up to 100 g
Example 2

FC from biomass pcs. IFRZh-5 10.0 g
Sorbitol food 2.0 g
Ethyl alcohol 50.0 g
Peppermint Oil 0.1 g
Food flavoring 0.5 g
Water dist. Up to 100 g
Example 3

FC from biomass pcs. VSKKKVR N 26 6.0 g
Ethyl alcohol 40.0 g
Citric acid 0.05 g
Xylitol food 1.0 g
Food flavoring 0.2 g
Water distill. Up to 100 g
Example 4

FC from biomass pcs. VSKKKVR N 24 10.0 g
Menthol 0.75 g
Polyvinylpyrrolidone 0.3 g
Food flavoring 0.2 g
Water dist. Up to 100 g
Essential aromatic food essences according to OST 18-103-84 mint and lemon were used as food flavorings.

 The inventive compositions were tested on 32 people. suffering from gum disease. They used rinses after brushing their teeth for 30 to 35 days.

 The compositions were well evaluated for taste, cosmetic, cleansing properties, the overall score of 4.6 ± 0.31 points.

 67% of the subjects noted the absence of bleeding of the gums after 5 7 days after applying the composition, pain in the gums was significantly reduced. After 20 22 days, these phenomena were already noted by 98% of the subjects. Conventional indices have confirmed this.

Hygiene index: before application 2.69 ± 0.27, after 1.65 ± 0.28
PMA index (5): before use 32.6 ± 1.8, after 7.9 ± 0.8.

 Thus, the proposed compositions are simple and accessible in production and use, the enzyme complex contained in them, obtained from natural raw materials, makes them highly effective, with anti-inflammatory, anti-allergic activity. Compounds cleanse teeth well, have beneficial effects on periodontal tissues. Good results were obtained in 98% of the subjects. They can be excellent oral hygiene and periodontal disease prevention products used at home.

Claims (4)

 1. The composition for the prevention of periodontal disease, which is an aqueous solution containing enzymes and target additives as an active component, characterized in that it contains an enzyme complex from biomass of cultivated ginseng or polyscias plant cells with the following content, wt. Enzyme complex 3.0 10.0
Target additives 0.1 2.6
Water or water-alcohol solution
2. The composition according to p. 1, characterized in that it contains a water-alcohol solution of the concentration of water alcohol (1 0.75) (1 2.5).  3. The composition according to p. 1, characterized in that as the target additive contains food flavoring in an amount of 0.2 to 0.5 wt.  4. The composition according to p. 1, characterized in that as the target additive contains perfumes, for example menthol or peppermint oil, in an amount of 0.1 to 0.75 wt.  5. The composition according to p. 1, characterized in that as the target additive contains sorbitol, or xylitol, or polyvinylpyrrolidone in an amount of 0.3 to 2.0 wt.