RU2058149C1 - Agent for ascospherosis and touch of decay disease treatment in bees - Google Patents

Abstract

FIELD: agriculture. SUBSTANCE: agent has antifungal substance - derivatives of naphthophenanthridine and chelerithrine, antibiotics imanine or tetracycline and the special additions - propolis and vitamin E dissolved in 1 ml alcohol. Preparation is used as an emulsion. EFFECT: enhanced effectiveness of agent proposed. 2 cl, 1 tbl

Description

 The invention relates to agriculture, in particular for the treatment of bees from ascospherosis and rotten diseases, which allows bee colonies to be preserved from death and allows cured families to produce products, i.e. honey.

 Due to the fact that bees are simultaneously affected by ascospherosis and rotten diseases, we have expanded the range of application of Dicobin R.

 The aim of the invention is to obtain "Dicobin R" from plant materials as a more environmentally friendly preparation in the treatment of both ascospherosis and rotten diseases of bees.

To achieve this goal, the following composition of substances is proposed: an antifungal substance of plant origin (C ₂₀ H ₁₄ O ₄ N ⁺ • SO ₄ H ^- ) and (C ₂₁ H ₁₈ O ₄ N + SO ₄ H ^- ) derivatives of naphthophenanthridine and heletrin, antibiotics tetracycline or of plant origin imanine, excipients: propolis extract, vitamin E, surfactants (surfactants) contained in 1 ml of an alcohol solution in the following ratio of components: Naphthophenanthridine or heleritrin (helerythrin) 10-150 mg Tetracycline 50-100 thousand units . Imanine 10-30 mg Propolis 10-20 mg Vitamin E 0.1-0.2 mg Surfactant 5-10 mg
The drug is used in the form of an aqueous emulsion contained in 250 ml of distilled or boiled water. The emulsion of an apolizing color or light milky color with a specific odor is stable for 2 days.

 For the study of acute toxicity and in accordance with the classification of toxicity of substances, the drug, i.e. derivatives of naphthophenanthridine and chelerythrin are low-toxic substances.

The method of obtaining the drug "Dicobin R" consists in dissolving in alcohol a powder (C ₂₀ H ₁₄ O ₄ N ⁺ • SO ₄ H ^- ) or (C ₂₁ H ₁₈ O ₄ N ⁺ • SO ₄ H ^- ) and a surfactant tetracycline or imanine, vitamin E and propolis. The mixture is sealed in ampoules of 1-2 ml. Dilution of the contents of the ampoule with distilled or boiled water is carried out immediately before treatment with the apiary.

 An example of the preparation of the drug.

Preparation 1. Derivatives of naphthophenanthridine 10 mg
with M.F. С ₂₀ Н ₁₄ О ₄ N ⁺ • СО ₄ Н ^- , mg: Imanin 10 Propolis 10 Vitamin Е 0.1 Ethyl alcohol 1 Oxanol ЦС (or syndanol ДС-10) 5
Dissolve in 70 ^about ethanol and sealed in an ampoule (1 ml).

Preparation 2. Derivatives of cheleritrin 10 mg:
s M.F. С ₂₁ Н ₁₈ О ₄ N ⁺ • SO ₄ Н ^- Imanin 10 mg Propolis 10 mg Vitamin E 0.1 mg Ethyl alcohol 1 ml
Oxanol CS (or syndanol DS-10) 5 mg
Dissolve in 70 ^about ethanol and sealed in an ampoule (1 ml).

The drug 3. A mixture of derivatives of naphthophenanthridine and cheleritrin in an amount of 10 mg: Tetracycline 100 thousand units Propolis 10 mg Vitamin E 0.1 mg Ethyl alcohol 1 ml Oxanol CS (or syndanol DS-10) 5 mg
Dissolve in 70 ^about ethanol and sealed in an ampoule (1 ml).

Preparation 4. A mixture of derivatives of naphthophenanthridine and choleritrin in an amount of 20 mg: Imanin 30 mg Propolis 10 mg Vitamin E 0.2 mg Oxanol 10 mg Ethyl alcohol 1 ml
Oxanol TsS-100 (GOST 614-102987) high molecular weight ether of the formula:
C _n H _{2n + 1} O (C ₂ H ₄ O) _m H, where n 16, m 100.

Dissolve in 70 ^about ethanol and sealed in an ampoule (1 ml).

The drug 5. A mixture of derivatives of naphthophenanthridine and choleritin in an amount of 150 mg: Tetracycline 100 thousand units Propolis 20 mg Vitamin E 0.2 mg Sindanol DS-10 10 mg Ethyl alcohol 1 ml
Dissolve in 70 ^about ethanol and sealed in an ampoule (1 ml).

To compare the proposed drug "Dicobin R" with the famous "Dicobin B", 10 bee colonies (one group) were infected with ascospherosis and rot (European and American), which were treated only with "Dicobin R", and 10 bee colonies, which were treated only " Dicobin B ". Processing was carried out at an air temperature not lower than +14 ^о С, in the evening, when all the bees are in the hives.

PRI me R 1. The drug "Dicobin R" is used as follows. 1 ml of an alcoholic solution of the drug, which contains (C ₂₀ H ₁₄ O ₄ N ⁺ • SO ₄ H ^- ) - derivatives of naphthophenanthridine 10 mg, imanine 10 mg, propolis 10 mg, vitamin E 0.1 mg (oxanol or syndanol DS-10 ) (preparation 1), dissolved in 250 ml of distilled or boiled water and get a stable emulsion of light milk color.

 The drug is poured into a Rosinka type sprayer and the frames are sprayed with the bee brood and bees. The consumption of the drug "Dicobin R" per frame 3-7 ml of emulsion, the treatment of bees is carried out with an interval after each treatment of 6-7 days. Processed 3 times.

 The drug "Dicobin B" is used as follows. 1 ml or 800 mg of a 1-2% alcohol extract of propolis, which contains 20 mg of αβ-dibromo-β-nitroethylbenzene, tetracycline (or rosamycin) 100000 units. Vitamin E 0.1 mg and 5 mg Sindanol DS-10, dissolved in 250 ml of distilled water and get a stable milky emulsion. The drug is poured into a Rosinka type sprayer and the frames are sprayed with the bee brood and bees. The consumption of the drug "Dicobin B" per frame 3-7 ml of emulsion, treatment is carried out with an interval after each treatment of 6-7 days. Processed 3 times.

 The processing results are presented in the table. As can be seen from the table, families treated with Dicobin R and Dicobin B and suffering from both ascospherosis and rotten diseases recover completely, and upon further observation they were not exposed to these diseases.

PRI me R 2. 1 ml of an alcohol solution containing derivatives of cholerythrin C ₂₁ H ₁₈ O ₄ N ⁺ • SO ₄ H ^- and naphthophenanthridine C ₂₀ H ₁₄ O ₄ N ⁺ • SO ₄ H ^-
150 mg, tetracycline 100,000 units. propolis 20 mg, vitamin E 0.2 mg, syndanol DS-10 10 mg. The drug is used according to the method of example 1, as a comparison, use the industrial drug "Dicobin B".

 Based on the data in the table, it was found that when treated with the drug "Dicobin R" and the drug "Dicobin B", the bees are completely cured of ascospherosis and decay diseases.

 Thus, Dicobin R, a herbal preparation that is environmentally friendly and harmless to the body of bees and humans, is an effective tool in treating bees from ascospherosis and decay diseases and can be used in any summer months, with no outbreaks of ascospherosis and rotten diseases of bees that have been treated.

Claims (1)

1. An agent for the treatment of ascospherosis and rotten diseases of bees, including an antifungal substance, an antibiotic and targeted additives, characterized in that as an antifungal substance it contains a mixture of derivatives of naphthophenanthridine C _{2 0} H _{1 4} O ₄ N ⁺ So ₄ H ^- and heleritrin C _{2 1} H _{1 8} O ₄ N ⁺ So ₄ H ^- , as an antibiotic tetracycline or imanine, and as target additives, propolis extract, vitamin E and surfactant dissolved in 1 ml of ethyl alcohol, in the following ratio, mg :
A mixture of naphthophenanthridine derivatives of the formula C _{2 0} H _{1 4} O ₄ N ⁺ So ₄ H ^- and chelerythrin of the formula C _{2 1} H _{1 8} O ₄ N ⁺ So ₄ H ^- 10.0 150.0
Tetracycline 100000 units
or Imanin 10 30
Propolis Extract 10 20
Surfactant 0.1 0.2
Vitamin E 5.0 10.0
2. The tool according to p. 1, characterized in that as a surfactant using a high molecular weight ester of the formula
C _n H _{2 n - 1} O (C ₂ H ₄ O) _m H,
where n 10 20;
m 20 100.

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