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RU2021123421A - Автоматизированные способы обработки клеток, модули, инструменты и системы - Google Patents

Автоматизированные способы обработки клеток, модули, инструменты и системы Download PDF

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RU2021123421A
RU2021123421A RU2021123421A RU2021123421A RU2021123421A RU 2021123421 A RU2021123421 A RU 2021123421A RU 2021123421 A RU2021123421 A RU 2021123421A RU 2021123421 A RU2021123421 A RU 2021123421A RU 2021123421 A RU2021123421 A RU 2021123421A
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module
automated
editing tool
alone multi
modular cell
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Дон МАСКЕЛЬЕ
Филлип БЕЛГРЕЙДЕР
Хорхе БЕРНАТЕ
Райан ДЖИЛЛ
Кевин НЕСС
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Инскрипта, Инк.
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Claims (22)

1. Автоматизированный автономный многомодульный инструмент для редактирования клеток для осуществления направляемого нуклеазой редактирования в клетках млекопитающих, включающий:
корпус, выполненный с возможностью размещения всех или некоторых модулей; емкость, выполненную с возможностью приема клеток млекопитающих; одну или более емкостей, выполненных с возможностью приема векторов экспрессии, где векторы экспрессии остов вирусного вектора и редактирующую кассету; модуль для выращивания клеток для трансфекции; модуль для трансфекции, выполненный с возможностью введения векторов в клетки млекопитающих; модуль для направляемого нуклеазой редактирования, выполненный с возможностью для введенных векторов экспрессии редактировать нуклеиновые кислоты в клетках млекопитающих; процессор, выполненный с возможностью обеспечения работы автоматизированного многомодульного инструмента для редактирования клеток, основываясь на вводе данных пользователем и/или выборе пользователем предварительно подготовленного скрипта; и автоматизированную систему подачи жидкости для перемещения жидкостей из емкости с клетками в модуль для выращивания, из модуля для выращивания в модуль для трансформации и из модуля для трансформации в модуль для направляемого нуклеазой редактирования без участия пользователя.
2. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, где автоматизированная система подачи жидкости содержит сиппер или дозатор.
3. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, где модуль для направляемого нуклеазой редактирования является также модулем восстановления.
4. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, дополнительно включающий модуль восстановления, отдельный от модуля редактирования.
5. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, где модуль для выращивания отделен от модуля для трансфекции.
6. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, где модуль для выращивания и модуль для трансфекции являются одним и тем же модулем.
7. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, дополнительно содержащий модуль для отбора.
8. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 7, где модуль для отбора является тем же модулем, что и модуль для направляемого нуклеазой редактирования.
9. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 1, где модуль для трансфекции обеспечивает условия для вирусной трансдукции.
10. Автоматизированный автономный многомодульный инструмент для редактирования клеток для осуществления направляемого нуклеазой редактирования в клетках млекопитающих, включающий:
корпус, выполненный с возможностью размещения всех или некоторых модулей; емкость, выполненную с возможностью приема клеток млекопитающих; одну или более емкостей, выполненных с возможностью приема векторов экспрессии, где векторы экспрессии содержат остов вирусного вектора и редактирующую кассету; модуль для выращивания клеток для трансфекции; модуль для трансфекции, выполненный с возможностью введения векторов в клетки млекопитающих; модуль для направляемого нуклеазой редактирования, выполненный с возможностью для введенных векторов экспрессии редактировать нуклеиновые кислоты в клетках млекопитающих; процессор, выполненный с возможностью обеспечения работы автоматизированного многомодульного инструмента для редактирования клеток, основываясь на вводе данных пользователем и/или выборе пользователем предварительно подготовленного скрипта; и автоматизированную систему подачи жидкости для перемещения жидкостей из емкости с клетками в модуль для выращивания, из модуля для выращивания в модуль для трансформации и из модуля для трансформации в модуль для направляемого нуклеазой редактирования без участия пользователя.
11. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где автоматизированная система подачи жидкости содержит сиппер или дозатор.
12. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где модуль для направляемого нуклеазой редактирования является также модулем восстановления.
13. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, дополнительно включающий модуль восстановления, отдельный от модуля редактирования.
14. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где модуль для выращивания отделен от модуля для трансфекции.
15. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где модуль для выращивания и модуль для трансфекции являются одним и тем же модулем.
16. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, дополнительно содержащий модуль для отбора.
17. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 16, где модуль для отбора является тем же модулем, что и модуль для направляемого нуклеазой редактирования.
18. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где модуль для трансфекции обеспечивает условия для липофекции.
19. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где модуль для трансфекции обеспечивает условия для магнитофекции.
20. Автоматизированный автономный многомодульный инструмент для редактирования клеток по п. 10, где модуль для трансфекции обеспечивает условия для микроинъекции.
RU2021123421A 2017-06-30 2018-06-30 Автоматизированные способы обработки клеток, модули, инструменты и системы RU2021123421A (ru)

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US201762527339P 2017-06-30 2017-06-30
US62/527,339 2017-06-30
US201762551069P 2017-08-28 2017-08-28
US62/551,069 2017-08-28
US201762566375P 2017-09-30 2017-09-30
US201762566374P 2017-09-30 2017-09-30
US62/566,374 2017-09-30
US62/566,375 2017-09-30
US201762566688P 2017-10-02 2017-10-02
US62/566,688 2017-10-02
US201762567697P 2017-10-03 2017-10-03
US62/567,697 2017-10-03
US201862620370P 2018-01-22 2018-01-22
US62/620,370 2018-01-22
US201862648130P 2018-03-26 2018-03-26
US62/648,130 2018-03-26
US201862649731P 2018-03-29 2018-03-29
US62/649,731 2018-03-29
US201862657651P 2018-04-13 2018-04-13
US201862657654P 2018-04-13 2018-04-13
US62/657,651 2018-04-13
US62/657,654 2018-04-13
US201862671385P 2018-05-14 2018-05-14
US62/671,385 2018-05-14
US201862689068P 2018-06-23 2018-06-23
US62/689,068 2018-06-23

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