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RU2015137666A - A method of cultivating yeast cells Phaffia rhodozyma to obtain a protein-vitamin supplement containing astaxanthin carotenoid - Google Patents

A method of cultivating yeast cells Phaffia rhodozyma to obtain a protein-vitamin supplement containing astaxanthin carotenoid Download PDF

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Publication number
RU2015137666A
RU2015137666A RU2015137666A RU2015137666A RU2015137666A RU 2015137666 A RU2015137666 A RU 2015137666A RU 2015137666 A RU2015137666 A RU 2015137666A RU 2015137666 A RU2015137666 A RU 2015137666A RU 2015137666 A RU2015137666 A RU 2015137666A
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RU
Russia
Prior art keywords
protein
phaffia rhodozyma
vitamin supplement
fermenters
cultivation
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Application number
RU2015137666A
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Russian (ru)
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RU2631803C2 (en
Inventor
Людмила Сергеевна Герман
Валентина Николаевна Сенаторова
Любовь Львовна Вакар
Ольга Аркадьевна Петрищева
Евгений Александрович Большаков
Лариса Дмитриевна Стехновская
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Людмила Сергеевна Герман
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Priority to RU2015137666A priority Critical patent/RU2631803C2/en
Publication of RU2015137666A publication Critical patent/RU2015137666A/en
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Publication of RU2631803C2 publication Critical patent/RU2631803C2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
    • A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)

Claims (2)

1. Способ культивирования дрожжей Phaffia rhodozyma для получения белково-витаминной добавки, содержащей астаксантин, включающий последовательное выращивание дрожжей: на твердой агаризованной среде, посевного материала на качалке в колбах, в ферментерах, в рабочем ферментере на основной ферментационной среде, содержащей источники углерода, ростовых факторов, минерального питания, отличающийся тем, что процесс культивирования в ферментерах проводят в отсутствие световой энергии, а для индукции каротиногенеза используют синглетный кислород, образованный в культуральной жидкости под действием сильного окислителя органической или неорганической природы, причем подачу раствора окислителя начинают по окончании лаг-фазы роста культуры и продолжают в течение всей экспоненциальной фазы роста, поддерживая постоянную оптимальную для данного окислителя концентрацию, обеспечивающую образование синглетного кислорода и каротиногенез в клетках дрожжей Phaffia rhodozyma.1. A method of cultivating the yeast Phaffia rhodozyma to obtain a protein-vitamin supplement containing astaxanthin, including sequential cultivation of yeast: on a solid agarized medium, seed on a rocking chair in flasks, in fermenters, in a working fermenter on a main fermentation medium containing carbon sources, growth factors, mineral nutrition, characterized in that the cultivation process in the fermenters is carried out in the absence of light energy, and singlet oxygen is used to induce carotenogenesis, about developed in the culture fluid under the action of a strong oxidizing agent of an organic or inorganic nature, moreover, the supply of an oxidizing solution begins at the end of the lag phase of growth of the culture and continues throughout the exponential growth phase, maintaining a constant optimal concentration for this oxidizing agent, which ensures the formation of singlet oxygen and carotenogenesis in cells yeast Phaffia rhodozyma. 2. Способ по п. 1, отличающийся тем, для получения белково-витаминной добавки с содержанием астаксантина не менее 50 мг/г, раствор окислителя подают в ферментеры на стадии получения посевного материала и на стадии основного культивирования по окончании лаг-фазы, в течение всего процесса культивирования, причем процесс культивирования ведут с постоянной подпиткой дополнительной ферментационной средой, в составе которой источником углерода является ферментативный гидролизат крахмала или целлюлозосодержащего сырья.2. The method according to p. 1, characterized in that to obtain a protein-vitamin supplement with an astaxanthin content of at least 50 mg / g, the oxidizing solution is fed to the fermenters at the stage of receiving the seed and at the stage of main cultivation at the end of the lag phase, during the whole cultivation process, and the cultivation process is carried out with constant replenishment of additional fermentation medium, in which the carbon source is an enzymatic hydrolyzate of starch or cellulose-containing raw materials.
RU2015137666A 2015-09-04 2015-09-04 Method for cultivation of phaffia rhodozyma yeast cells for production of protein-vitamin additive containing astaxanthin carotinoid RU2631803C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2015137666A RU2631803C2 (en) 2015-09-04 2015-09-04 Method for cultivation of phaffia rhodozyma yeast cells for production of protein-vitamin additive containing astaxanthin carotinoid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2015137666A RU2631803C2 (en) 2015-09-04 2015-09-04 Method for cultivation of phaffia rhodozyma yeast cells for production of protein-vitamin additive containing astaxanthin carotinoid

Publications (2)

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RU2015137666A true RU2015137666A (en) 2017-03-10
RU2631803C2 RU2631803C2 (en) 2017-09-26

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RU2015137666A RU2631803C2 (en) 2015-09-04 2015-09-04 Method for cultivation of phaffia rhodozyma yeast cells for production of protein-vitamin additive containing astaxanthin carotinoid

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113331318A (en) * 2021-06-07 2021-09-03 浙江博仕佳生物科技有限公司 Preparation method of feed rich in yeast, astaxanthin, lactic acid and protease for shrimp and crab culture
CN113907051A (en) * 2021-12-03 2022-01-11 遵义市林业科学研究所 A kind of artificial rearing method of prickly ash

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2385925C1 (en) * 2008-10-22 2010-04-10 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП ГосНИИгенетика) Phaffia rhodozyma YEAST STRAIN - ASTAXANTHIN PRODUCER
RU2529715C2 (en) * 2011-03-22 2014-09-27 Общество с ограниченной ответственностью "ФБТ" Method of cultivating phaffia rhodozyma yeasts for obtaining astaxanthin-containing forage additive

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113331318A (en) * 2021-06-07 2021-09-03 浙江博仕佳生物科技有限公司 Preparation method of feed rich in yeast, astaxanthin, lactic acid and protease for shrimp and crab culture
CN113331318B (en) * 2021-06-07 2024-02-20 浙江博仕佳生物科技有限公司 Preparation method of feed for enriching yeast, astaxanthin, lactic acid and protease for shrimp and crab culture
CN113907051A (en) * 2021-12-03 2022-01-11 遵义市林业科学研究所 A kind of artificial rearing method of prickly ash

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Effective date: 20180120