RU2013950C1 - Means for stimulating reproductive function and growth of animals - Google Patents

Abstract

FIELD: agriculture. SUBSTANCE: means is composed of tissue extract from intestines of Japanese trepang (Stichopus Japonicas). Preparation is administered in the form of 1,2 and 3 daily injections in a dose of 0.1 ml per kg of body weight. Chickens receive the preparation with their regular feed in a dose of 0.3 ml per capita per day. Growth-stimulating effect of the preparation depends on kind of animal. It is 6.6 % for chickens, 22 % for white mice and 17% for calves. Two-fold injection of extract to cows favours earlier development of sexual cycles and increases fertilization from 16.3 to 22 %. Three-fold injection of the preparation stimulates the sexual cycles and normalizes reproductive function in cows that were down with endometritis. EFFECT: higher efficiency. 6 tbl

Description

 The invention relates to agriculture / in particular to livestock and can be used to stimulate the reproductive function and growth of animals.

 Among the many means of stimulating the vital functions of the animal organism, tissue preparations / manufactured according to the method of V.P. Filatova / lysates / hydrolysates and ASD.

 Tissue preparations are also used to treat various diseases. Moreover, they are effective for chronically occurring diseases [1].

 In recent years, tissue preparations are recommended to be used as part of complex treatment regimens for various diseases of the reproductive organs of cows (Prevention and treatment of diseases of farm animals. Collection of scientific papers Odessa / 1972).

 The aim of the invention is to increase the reproductive function and growth of animals. At the same time, the problem of non-waste production technology and / as a result / environmental protection was solved.

 This goal is achieved by using tissue extract from the guts of Japanese trepang (Stichpus japonicus) as a means to stimulate reproductive function and growth of animals.

 The internal organs of Japanese trepang contain enzymes / biologically active substances / lipids / carbohydrates / various trace elements / prostaglandins / amino acids and nucleic acids [2]. The internal organs of Japanese trepang / as a waste of the fishing industry / have a rich chemical composition.

 The preparation was prepared according to the method of V.P. Filatov (the waste / obtained from the processing of Japanese trepang / was collected in plastic bags and placed in the refrigerator for 6-7 days. Then they were passed through a meat grinder / weighed and poured with physiological saline at a rate of 5 liters per 1 kg of minced meat The resulting mass was thoroughly mixed / poured into a bottle / tightly corked and placed in a refrigerator for extraction / in a day the mass was filtered / the resulting extract was boiled for 9 minutes, the cooled suspension was centrifuged at 5000 rpm for 30 min. The supernatant was filled into sterile 250 ml vials and closed with cotton-gauze plugs. The bottles with the preparation were autoclaved at 1/5 atm for an hour. Then, under sterile conditions, the cotton-gauze plugs were replaced with rubber ones and filled with tar.

 The biological activity of tissue extract was determined according to the method of V. Filatov in the modification of V. Roshchina.

 The activity of the drug ranged from 132/0 to 200%.

 The growth-promoting effect of the drug was tested on white mice / chickens / heifers and cattle gobies. The results are presented in the following tables.

 The results of scientific and economic experiments show / that the animals of the experimental groups under the same conditions of feeding and keeping had a greater increase in live weight compared to the control. For white mice, this difference was 22%.

 Feeding the drug to chickens in the amount of 0/3 ml per head per day increased the average daily growth during the first month of life by 6/6%.

 With a single injection of the extract to heifers at a dose of 0/1 ml per kg of live weight, the difference in the gain in live weight was 17%.

 Two experiments were performed on the cows. For the first, we selected two groups of analogues of 10 goals / taking into account the date of birth / calving / milk productivity / live weight. Feeding animals is general / identical for all groups. The diet consisted of hay / hay stock / silage / concentrates / grass meal / root crops / bards. The test results of the drug to stimulate the reproductive function of cows are presented below.

 Twice (after 7 days) injections of 20 ml of the extract contributed to the earlier manifestation of reproductive cycles and increased fertility by 20% in the first experiment and in the second by 16/3%.

 When the drug was administered to 5 spring-fed cows (the average time interval from calving to the start of treatment was 173/8 days), hunting occurred on average after 35 days / fertilization -57/0 days / 100% of the cows were fertilized in three months / in the control group, respectively 132 / 3; 55.6; 78.6; 33.0%.

 We also studied the effect of the extract from the viscera of trepang to stimulate hunting in cows / with acute and chronic forms of endometritis. Animals were monitored for 9 months. The drug was administered subcutaneously at a dose of 20 ml three times after 7 days. Clinical observations were conducted daily until insemination.

 Of the 27 cows / being treated / 6 were handed over to the meat factory due to / not related to gynecological diseases.

 Of the 8 animals with a long absence of reproductive cycles (128/4) in 3 hours, they did not recover during the entire observation period. In the remaining 5 cows, reproductive cycles resumed after 31 days from the start of treatment.

 Of the 5 cows / who came to the hunt in the first 60 days, 3 were fertilized / and in the three months from the start of treatment - 4 / or 80%.

 In 13 cows, an average of 42/5 days passed from calving to the start of drug administration. Three were handed over to the meat factory. On average, in the 10 remaining animals, the first hunt came after 37/7 days / of which 2 cows were fertilized in the first month / in the second - 3 / in only 90 days from the start of treatment, 7 cows (70%). Two cows were taken for treatment 162 days after calving / due to multiple unsuccessful inseminations. The first injection of the drug was made 20 days after coating. After 8 days, they again went hunting and fertilized.

 Three-time administration of the drug from the entrails of trepang stimulates the manifestation of reproductive cycles and normalizes reproductive function in cows / patients with endometritis.

 A study of the biochemical composition of the blood serum of cows did not reveal significant changes in connection with the introduction of the studied drug into the body.

 Along with the study of the biological activity of a tissue preparation / its chemical composition has been studied (see Appendix).

 The use of tissue extract from the entrails of trepang as a means to stimulate the reproductive function and growth of animals contributes / ultimately / to an increase in livestock production and improve its quality.

Claims (1)

 The use of tissue extract from the innards of Japanese trepang (Stichpus japonicus) as a means to stimulate reproductive function and growth of animals.

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