RU2013946C1 - Plant multiplication method - Google Patents

Abstract

FIELD: agriculture. SUBSTANCE: method involves obtaining sprout in tissue culture on artificial nutrient medium; preparing growth medium, sterilizing it in cultivation pot; planting sprout in growth medium to provide rooting and growing of plant; providing microcutting from plant, when it reaches the height exceeding 6 cm, and planting it in sterile growth medium to provide rooting. The remaining part of plant is cultivated so that it may be used for repeated cutting or the remaining part of plant is removed from cultivation pot and transplanted to permanent site for growing. EFFECT: increased efficiency and high quality of plants grown from cuttings obtained by such method. 2 cl, 4 tbl

Description

 The invention relates to agriculture / in particular to crop production / and can be used in nursery for the production and propagation of healthy planting material for fruit crops.

 A known method of growing garden plants / consisting of planting a shoot on an artificial nutrient medium / containing cytokinin / auxin and phloroglucinol to obtain several shoots / cutting individual shoots for rooting on the same or similar medium in the absence of cytokinin / subsequent planting of plants in pots with soil substrate / placing the latter in moist plant boxes and subsequent hardening of plants by reducing humidity in the boxes to obtain viable seedlings.

 There is also known a method of plant micropropagation / according to which, when transferring planting material from an in vitro state to an in vivo state, its viability is increased by introducing 0 / 001-10 / 0 mg / l paclobutrazole into the composition of the nutrient medium / and the plant material is cultivated in contact with solid porous carrier / placed in a liquid nutrient medium.

 The closest technical solution known is the method of growing plants / consisting in propagating shoots on an artificial nutrient medium with cytokinin / cutting and transplanting the shoots on a liquid nutrient medium with auxin to form root primordia / subsequent planting of shoots on peat briquettes / mounted on a tray / room of the latter moist plant boxes or greenhouses to adapt plants to non-sterile conditions.

 The disadvantage of this method is the significant complexity due to several technological operations / death of some plants due to exposure to pathogenic microflora / need for expensive equipment.

 The aim of the present invention is to increase the reproduction rate by reducing the time of growing plants and the rational use of plant material / reduce material and labor costs by combining and reducing technological operations.

 The essence of the invention lies in the fact that after accelerating the shoot in a cultivation vessel with a substrate and developing the stem part of a plant larger than 6 cm from it, microcranes are harvested for subsequent rooting / and then the microcheren part of the plant stem with roots remaining after harvesting is extracted from the cultivation vessel together with the substrate and planted in open ground or not removed from the cultivation vessel and used to obtain a new microcranium. Using the proposed method will allow for 2-4 months to accelerate the production of plants / in comparison with the prototype and analogues / through the use / starting from the second passage / shoots / obtained in vitro and combining the stage of accelerating the shoots of the stage of adaptation of microplants in one operation / to achieve 90-100 % survival of plants in non-sterile conditions / to ensure the production of identical plants with a multiplication factor of 1000: 1 / for 1 year, provided that it starts with a single sterile shoot 4-5 cm long (Table 1/2/3/4).

+2^x-7+2^x-8+ где x - число месяцев размножения/ при x ≥ 3. Коэффициент размножения за год будет равен:

2

2⁷+2⁶+2⁵+2⁴+2³+
Способ осуществляется следующим образом. На искусственной питательной среде выращивают побег длиной 4-6 см. Подготавливают культивационный сосуд/ например банку или стакан из термостойкого материала емкостью 150-200 мл. Субстрат приготавливают из торфа/ песка или перлита в соотношении 1:1:1/ увлажняют его/ заполняют им культивационный сосуд/ накрывают фольгой и стерилизуют в автоклаве в течение 1 ч при давлении 2 атм. Обработанный таким образом культивационный сосуд с почвенным субстратом переносят в ламинарбокс/ где в фольге с помощью острого колющего предмета/ например шила/ выполняют отверстие/ через которое производят посадку побега. Побег предварительно обрабатывают ауксином путем замачивания в растворе или обмакивания в пудру или пасту. Далее на культивационный сосуд с высаженным в него побегом устанавливают вверх дном прозрачный сосуд-покрытие аналогичного размера для предотвращения высыхания побега и герметизируют места соприкосновения сосудов эластичным материалом/ например липкой лентой. Через 4-5 нед после высадки побега получают стерильное корнесобственное растение с длиной стебля 6-9 см. С такого растения в условиях ламинар-бокса заготавливают микрочеренок размером 4-5 см и высаживают его аналогично начальному побегу. Оставшуюся после заготовки микрочеренка часть стебля растения с корнями извлекают из культивационного сосуда вместе с почвенным субстратом и высаживают непосредственно в открытый грунт. Кроме того/ оставшуюся поле заготовки микрочеренка часть стебля растения можно не извлекать из культивационного сосуда и после подкормки стерильным раствором минеральных удобрений/ установки сосуда-покрытия/ как указано выше/ использовать для получения нового микрочеренка.The calculation of the potential breeding coefficient is carried out according to the formula:

+2 ^x-7 +2 ^x-8 + where x is the number of months of reproduction / for x ≥ 3. The reproduction rate for the year will be equal to:

2

2 ⁷ +2 ⁶ +2 ⁵ +2 ⁴ +2 ³ +
The method is as follows. A shoot 4-6 cm long is grown on an artificial nutrient medium. A cultivation vessel is prepared / for example a jar or a glass of heat-resistant material with a capacity of 150-200 ml. The substrate is prepared from peat / sand or perlite in a ratio of 1: 1: 1 / moisten it / fill it with a cultivation vessel / cover with foil and sterilize in an autoclave for 1 h at a pressure of 2 atm. The cultivating vessel with the soil substrate thus treated is transferred to the laminarbox / where in the foil with the help of a sharp piercing object / for example an awl / a hole is made / through which the shoot is planted. The shoot is pretreated with auxin by soaking in a solution or dipping in powder or paste. Next, a transparent vessel of the same size is installed upside down on the cultivation vessel with the shoot planted in it to prevent the shoot from drying out and the junction of the vessels is sealed with an elastic material / for example, adhesive tape. In 4-5 weeks after the shoot is planted, a sterile root plant with a stem length of 6-9 cm is obtained. From such a plant, micro-stalks of 4-5 cm in size are harvested under the conditions of a laminar box and planted similarly to the initial shoot. The part of the plant stem with roots that remains after the microcrop is harvested is extracted from the cultivation vessel together with the soil substrate and planted directly in the open ground. In addition / the remaining micropotent harvesting field part of the plant stem can not be removed from the cultivation vessel and after feeding with a sterile solution of mineral fertilizers / installation of the coating vessel / as described above / use to obtain a new microproject.

Claims (2)

 1. METHOD FOR PLANT REPRODUCTION, including obtaining a shoot in a tissue culture on an artificial nutrient medium, preparing a substrate, sterilizing it in a cultivation vessel, planting a shoot in a substrate for rooting and growing a plant, characterized in that, after the plant reaches a height of more than 6 cm, it is harvested microcranes and root it in a sterile substrate.  2. The method according to claim 1, characterized in that the part of the plant remaining after microcherening is grown for re-cuttings or removed from the cultivation vessel and planted at a constant growth site.

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