RU2008115281A - MICROBIOLOGICAL METHOD FOR PRODUCING 7ALPA-HYDROXYANDROSTENES - Google Patents
MICROBIOLOGICAL METHOD FOR PRODUCING 7ALPA-HYDROXYANDROSTENES Download PDFInfo
- Publication number
- RU2008115281A RU2008115281A RU2008115281/13A RU2008115281A RU2008115281A RU 2008115281 A RU2008115281 A RU 2008115281A RU 2008115281/13 A RU2008115281/13 A RU 2008115281/13A RU 2008115281 A RU2008115281 A RU 2008115281A RU 2008115281 A RU2008115281 A RU 2008115281A
- Authority
- RU
- Russia
- Prior art keywords
- hydroxyandrostenes
- producing
- substrate
- mycelium
- microbiological method
- Prior art date
Links
- 238000013048 microbiological method Methods 0.000 title claims abstract 3
- 229920000858 Cyclodextrin Polymers 0.000 claims abstract 4
- 150000003431 steroids Chemical class 0.000 claims abstract 4
- 239000000758 substrate Substances 0.000 claims abstract 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract 2
- 241000223211 Curvularia lunata Species 0.000 claims abstract 2
- 239000001116 FEMA 4028 Substances 0.000 claims abstract 2
- 102000008109 Mixed Function Oxygenases Human genes 0.000 claims abstract 2
- 108010074633 Mixed Function Oxygenases Proteins 0.000 claims abstract 2
- 230000004913 activation Effects 0.000 claims abstract 2
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims abstract 2
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims abstract 2
- 229960004853 betadex Drugs 0.000 claims abstract 2
- 230000015572 biosynthetic process Effects 0.000 claims abstract 2
- 229940097362 cyclodextrins Drugs 0.000 claims abstract 2
- 230000033444 hydroxylation Effects 0.000 claims abstract 2
- 238000005805 hydroxylation reaction Methods 0.000 claims abstract 2
- 238000000034 method Methods 0.000 claims abstract 2
- 239000013081 microcrystal Substances 0.000 claims abstract 2
- 235000015097 nutrients Nutrition 0.000 claims abstract 2
- 230000009466 transformation Effects 0.000 claims abstract 2
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
1. Микробиологический способ получения 7α-гидроксиандростенов с помощью грибного мицелия, отличающийся тем, что, с целью увеличения нагрузки стероидного субстрата и скорости его гидроксилирования, трансформацию стероидов проводят с помощью мицелия Curvularia lunata ВКПМ F-981, без предварительной активации фермента гидроксилазы и в отсутствие компонентов питательной среды, а субстрат используют в виде микрокристаллов, либо в виде комплекса с циклодекстринами. ! 2. Способ по п.1, отличающийся тем, что для образования комплексов используют β-циклодекстрин или его химически модифицированные производные, такие как метилциклодекстрин и гидроксипропилциклодекстрин.1. Microbiological method for producing 7α-hydroxyandrostenes using mushroom mycelium, characterized in that, in order to increase the load of the steroid substrate and the rate of its hydroxylation, the transformation of steroids is carried out using Curvularia lunata VKPM F-981 mycelium, without prior activation of the hydroxylase enzyme and in the absence of components of the nutrient medium, and the substrate is used in the form of microcrystals, or in the form of a complex with cyclodextrins. ! 2. The method according to claim 1, characterized in that for the formation of complexes using β-cyclodextrin or its chemically modified derivatives, such as methylcyclodextrin and hydroxypropylcyclodextrin.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008115281/13A RU2377309C1 (en) | 2008-04-22 | 2008-04-22 | MICROBIOLOGICAL METHOD FOR PREPARATION OF 7α-HYDROXYANDROSTENES |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008115281/13A RU2377309C1 (en) | 2008-04-22 | 2008-04-22 | MICROBIOLOGICAL METHOD FOR PREPARATION OF 7α-HYDROXYANDROSTENES |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2008115281A true RU2008115281A (en) | 2009-10-27 |
| RU2377309C1 RU2377309C1 (en) | 2009-12-27 |
Family
ID=41352531
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2008115281/13A RU2377309C1 (en) | 2008-04-22 | 2008-04-22 | MICROBIOLOGICAL METHOD FOR PREPARATION OF 7α-HYDROXYANDROSTENES |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2377309C1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105733964B (en) * | 2016-04-27 | 2019-01-04 | 山东省农业科学院植物保护研究所 | A kind of mould culture medium of curved spore and preparation method thereof containing clearvers |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2771105A1 (en) * | 1997-11-20 | 1999-05-21 | Vitasterol | 7 alpha-hydroxylation of dehydroepiandrosterone and pregnelone by bioconversion using Fusarium moniliforme |
| NZ537871A (en) * | 2002-07-24 | 2006-10-27 | Schering Ag | Microbiological processes for the production of 7 alpha-substituted 11 alpha-hydroxy steroids |
-
2008
- 2008-04-22 RU RU2008115281/13A patent/RU2377309C1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| RU2377309C1 (en) | 2009-12-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| NO20065164L (en) | Germinated, particulate boemite material and process for making it. | |
| WO2009064200A3 (en) | Novel bacteria and methods of use thereof | |
| DK1891225T3 (en) | Step-by-step process for dry matter fermentation | |
| JP2010502191A5 (en) | ||
| WO2008123213A1 (en) | Semiconductor device and semiconductor manufacturing method | |
| CN105217683B (en) | Application of silver vanadate serving as mimic enzyme | |
| CN101020919A (en) | Biological sterol transforming process with cyclodextrin and its application | |
| BRPI0307349B8 (en) | process for fermenting a phytosterol composition | |
| EP2011861A8 (en) | Bacterium capable of producing purine substance, and process for production of purine substance | |
| PH12013501293A1 (en) | Use of nitrogen-free peroxygen-releasing compound to reduce growth of contaminant microorganisms in ethanol fermentation | |
| RU2008115281A (en) | MICROBIOLOGICAL METHOD FOR PRODUCING 7ALPA-HYDROXYANDROSTENES | |
| BR112014028957A2 (en) | processes for fermenting gaseous substrate containing carbon monoxide (co), for cultivating bacteria on selected substrate and for cultivating bacteria in synthesis gas | |
| CN103903966A (en) | Method for manufacturing ultrahigh-density germanium silicon quantum dots based on obliquely-cut silicon substrate | |
| EP1233071A3 (en) | Organic nitrogen-containing composition and fertilizer comprising the same | |
| ATE386527T1 (en) | INCLUSION COMPLEX OF TUBERCULOSTATIC RIFAMPICIN WITH BETA-CYCLODEXTRIN OR 2-HYDROXYPROPYL BETA-CYCLODEXTRIN AND A PROCESS FOR PRODUCING THE SAME | |
| CN107306462A (en) | A kind of LED battle array Audio presentation device and its Fourier modes fuzzy control method | |
| WO2011146714A3 (en) | Method and device using plasmon-resonating nanostructures | |
| WO2005118827A3 (en) | Fermentation process | |
| RU2015104355A (en) | METHOD FOR PRODUCING C2-OXYGENATES BY FERMENTATION USING SULFUR WITH A HIGH OXIDATION DEGREE | |
| WO2005086690A3 (en) | Mosfet amplifier having feedback controlled transconductance | |
| Nzeteu | Development of an enhanced chain elongation process for caproic acid production from waste-derived lactic acid and butyric acid | |
| MY152096A (en) | Pretreatment method for biomass saccharification and method for saccharification using the pretreatment method | |
| WO2011112295A3 (en) | Large-area, free-standing metal oxide films and transistors made therefrom | |
| TWD234393S (en) | Windshield | |
| CN207125548U (en) | A kind of stealthy tilting table of detachable auto-folder support feet |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20150423 |