RU2004215C1 - Apparatus for carrying out inhalation narcosis of small laboratory animals - Google Patents

Description

the nose hole of the chamber and the gate of the shutter are equipped with seals and on the upper wall of the chamber the transparent dropper is fixedly and tightly fixed, connected to the drug delivery system, consisting of a bottle for the narcotic substance, into which the short and long needles are inserted, communicated using two transparent elastic connecting tubes, respectively, with a supercharger and through a clamp-regulator of drug delivery with a dropper, while the means for fixing the animal is made in the form of a metal head holder of the animal coupling latch head holders having a locking screw, and rigidly fixed on the opposite wall of the inlet chamber, in which an aperture for the end of the head holders.

Comparative analysis with the prototype shows that the claimed device is characterized in that in order to improve its operational characteristics, it has a transparent anesthesia chamber fixedly mounted on a table, the volume of which is 14-15 times the volume of the head of the experimental animal, a movable shutter with a notch repeating the configuration of the animal’s neck, grooves on the side walls of the chamber entrance, guiding the movement of the shutter, the seal of the chamber entrance and shutter, a transparent sealed dropper, bottle for drug cat, long and short needles, syringe, transparent elastic connecting tubes, drug clamp, head holder, head holder lock, rigidly mounted on the front wall of the chamber

All this makes it possible to sharply reduce the likelihood of a drug overdose, c- abolishes the drug process (by reducing the rate of fluctuations in the concentration of the drug in the chamber), prolongs the narcotic effect of the portion of the drug introduced into the chamber, reduces the consumption of the drug, its effect on ex - a perimeter, makes the device convenient to use.

In FIG. 1 schematically shows the proposed device for inhalation anesthesia in small laboratory animals, general view, FIG. 2 - drug treatment chamber n table of this device, front view

The device contains an operating table 1, 2, a rectangular through hole 3 in its plane, fixed screws 4 on its surface, a transparent anesthesia chamber 5, directing the grooves 6, the shutter 7 with a bend 8, void; clear dropper 10 s. input-tube 11, bottle 12 for drugs, syringe 13, long 14 and short 15 needles, screw 5 clamp 16. two connecting transparent elastic tubes 17, 18. head holder 19, fixed arc 20 on it, movable sleeve 21, ring 22, lock screw 23, retainer holder 24, hole

0 in it and locking screw 26.

The device is made in the form of a table 1, 2, on the head end of which an anesthesia chamber 5 of a rectangular shape is fixedly fixed (glued), made 5 of transparent material (for example, plexiglass). This allows you to monitor the condition of the animal, determine the depth anesthesia for the movement of the eyelids, tongue and lower jaw. The front, top and side seams of chamber 5 are sealed (with glue). Its volume is 14-15 times greater than the head of the animal. This size of the chamber 5 allows sharply (in comparison with the mask fitting the head with the same amount

5) 14-15 times to reduce the risk of a toxic concentration of the narcotic substance occurring in it, makes it possible to freely enter the animal’s head and create its optimal concentration with a small dose of the drug (a further increase in the size of chamber 5 is impractical because it to an increase in drug consumption and slows the entry of animals into the deep narcotic phase). The rear end of the chamber 5, its entrance, is closed by a shutter 7. The dimensions of the entrance allow the head of the animal to be freely inserted through it. The shutter 7 moves vertically along the guide grooves 6 in the side walls of the chamber 5. There is a cutout 8 in the lower part of the shutter 7 the configuration repeats the configuration of the animal’s neck, Cutout 8 of the shutter 7, the upper 5 n and the lower part of the entrance to the chamber 5 are glued with a soft elastic seal 9 (for example, foam rubber or rubber) designed to seal the chamber 5 when the animal’s head and vehicle move in

0 her. Such a seal sharply reduces the exit of the drug outside the chamber 5. prolongs the narcotic effect of the dose of the drug introduced into the anesthesia chamber 5, reduces its consumption,

L reduces the effect of the drug on the experimenter. On the upper wall of the chamber 5, on the side, in the middle, a transparent dropper 10 (for example, from plexiglass) is motionlessly hermetically fixed, allowing counting drops of the drug being supplied.

the position is determined by the condition that the narcotic substance falls on a porous material - an evaporator (for example, cotton wool), located on the side of the animal’s head. The inlet tube 11 of the dropper is connected by a transparent elastic tube 17 (for example, of silicone, for monitoring drug intake, which reduces the possibility of overdose) with a long needle 14 through which the drug is delivered from the bottle 12 (it can be a standard drug bottle, manufactured by industry). To this end, the sharp end of the needle 14 passes through the stopper of the bottle 12 to its bottom. The drug is delivered due to the increased pressure in the vial 12 created by the syringe 13 (or pear). It is connected by a tube 18 to a short needle 15, the sharp end of which is passed through the stopper of the bottle 12 and is located above the narcotic liquid. The entry of the drug into the chamber 5 is regulated by a screw clamp 16. located on the tube 17. Such a drug supply system eliminates the experimenter's contact with this substance and makes it easy and accurate to dose it into the chamber 5 h. The outside of the front wall of the chamber 5 (Fig. 2) is stationary the head holder 24 is fixed with a locking screw 26 and a hole 25 matching the hole in the wall of the chamber 5. The hole 25 serves to guide and secure the head holder 19. It is glued with a sealant.

The presence of constructive functional connection between the table, camera and headband makes the device convenient to use.

The head holder 19 is designed to firmly fix the head of the animal. It consists of a metal rod, the head part of which is curved along the profile of the head of the rat, a metal arc 20, wound behind the head of the animal on its neck. Arc 20 is rigidly connected to the rod. A movable metal clutch 21 with a locking screw 20 and a fixed metal ring 22 is located on the rod. It fixes the upper jaw of the rat. Rigid fastening of the head of the experimental animal eliminates the possibility of the animal removing the head from the chamber in the case of superficial anesthesia and, therefore, prevents the disturbance of the drug process.

The surface 1 of the table and its fixed legs 2 (Fig. 1 and 2) are made of plexiglass or metal. A rectangular through hole 3 is cut in the middle of the table. Its width is equal to 1/3 of the width of the body of the animal, and its length is 1/2 the length of its body. Hole 3 improves fixation of the body. The upper back or lower abdomen falls there, which prevents the trunk from falling to the side. For fastening the extremities of the animal, screws 4 are located on the table, which are rigidly connected to its surface 1.

0 The device operates as follows.

Two needles 14, 15 are inserted into the drug vial 12, as shown in FIG. 1. A short needle 15 connects to a syringe

5 13, length 14 - with tube 17. Using a syringe 13 and a clamp 16 (preferably in a fume hood), the rate of drug delivery through the tube 17 is regulated. After adjustment, the drug remaining in the tube 17 is returned to the vial by reverse movement of the syringe piston 13. The free end of the tube 17 is put on the tube 11 of the dropper 10 (Fig. 1). The shutter 7 of the chamber 5 is pulled out upward. AT

5, a chamber 5 is placed under a dropper 10 with cotton or other porous material. An animal (e.g., rat, rabbit, guinea pig) undergoes preliminary inhalation anesthesia under a hood (preferably in a fume hood). Upon reaching a sufficient depth of anesthesia, as evidenced by the disappearance of corneal reflexes, it is removed from under the hood, Arc 20 of the head holder 19

5 winds behind the head of the animal until it stops. The ring 22 of the sleeve 21 is put on the upper jaw behind the incisors. In this position, the sleeve 21 is fixed to the head of the head 19 with a screw 23. The free end of the shaft

The head holder 19 is passed through the chamber 5, inserted into the hole 25 of the head holder 24, and pulled through it until the entire head of the animal and two-thirds of its neck are in the chamber 5.

5 After that, the head-holder shaft 19 is secured with a locking screw 26 (the animal's head can be positioned either with the crown of the head up or with the crown of the head depending on need). Damper 7 is inserted

0 into the grooves 6 of the walls of the chamber 5 and lowers down to the seal 9 at the bottom of the chamber inlet. Using a syringe 13, an increased pressure is created in the vial 12c with the drug, and 10-15 drops are introduced into the chamber 5

5 of this substance. Drops are counted through the transparent walls of the dropper 10. The remainder of the drug from the tube 17 is aspirated into the vial with a syringe 13. The paws of the animal are fixed to the screws 4 using threads. To do this (the screw cage is loosened by 2-3

turn, the free end of the thread is wrapped twice around the screw 4 in a clockwise direction and pressed by its head. When fixing the animal, it is positioned so that the back (or abdomen) is in the hole 3 of the table, Anesthesia is on the verge of movement of the animal (it is necessary to monitor the motility of the eyelids, lower jaw, tongue, limbs). When movements appear, 3-5 drops of the drug are added to chamber 5.

When carrying out inhalation anesthesia in rats, using the proposed device, 0.3 to 0.4 ml of ether is consumed per 1 hour.

Experimental studies and testing of the proposed device for inhalation anesthesia in small laboratory animals, in particular rats, showed that, in comparison with a device of a similar purpose (prototype), the invention has better performance due to:

1) using instead of a mask a transparent drug chamber, the volume of which is 14-15 times the volume of the head of the experimental animal. This significantly reduces the likelihood of a toxic concentration of the drug in the chamber, allows the head of the animal to be introduced into it freely, and makes it possible to monitor the depth of anesthesia according to the state of the organs of the head of the animal;

2) sealing the entrance to the chamber during anesthesia with a shutter and gaskets. AT

as a result, the release of the drug into the atmosphere sharply decreases, the rate of decrease of its drug concentration in the chamber, the drug’s state of the animal is stabilized and prolonged, the consumption of the drug substance and its effect on the experimenter are reduced;

3) the use of a sealed drug supply system in the chamber, consisting of

0 droppers, vial for narcotic substance, syringe, two needles and transparent connecting tubes, which prevents the experimenter from contacting the drug, reduces its consumption and simplifies

5 dosage of the drug;

4) rigid fixation of the head of the experimental animal with the help of a head restraint, which excludes the possibility of removing the animal’s head from the chamber in case of superficial anesthesia and, therefore, prevents the drug process from being disturbed;

5) the presence of a constructive relationship between the drug-treatment chamber and the operating table and head holder. The camera is fixedly mounted on the table, and the head restraint lock is located on its front wall. The chamber and the table form a single functional unit. It does

0 device is easy to use,

(56) Copyright certificate of the USSR No. 1017320, cl. A 61 D 7/00. 1983.

Claims (1)

SUMMARY OF THE INVENTION DEVICE FOR CARRYING OUT INHALATION ANNESTIC ANTISOPHY OF SMALL LABORATORY ANIMALS containing an anesthesia device with an inlet for the animal’s head and means for fixing the animal, characterized in that, in order to increase its performance by creating and maintaining an optimal concentration of the drug, control of the depth of anesthesia by changing the motility of the organs of the animal’s head, its rigid fixation and reducing the impact drugs for the experimenter narkotizatsionnoe device is designed as a permanently mounted on the operating table and enshrined transparent hermetically by seams chamber whose volume is 14 - 15 times the volume of the animal's head, and an inlet closed by a shutter, having a bottom recess corresponding to the configuration of the neck of the animal, and 0 5 installed with the possibility of vertical movement along the guide grooves made on the side walls of the inlet of the chamber, the inlet of the chamber and the cutout of the shutter are provided with seals, and a transparent dropper connected to the drug delivery system consisting of a fixed and fixed hermetic is fixed and sealed on the upper wall of the chamber from a vial for a narcotic substance, e which short and long needles are inserted, communicated by means of two transparent elastic connecting tubes respectively with a supercharger and a Res clamp-regulating drug delivery torus with a dropper, wherein the means for fixation of the animal is made as a sleeve and metallicheskogogolovoderzhatel latch head holders having a locking screw, and rigidly fixed on the opposite wall of the inlet chamber, in which an aperture for the end of the head holders. YU FIG. 1