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RU2002119320A - METHOD FOR DETERMINING THE PROTEIN CONTENT - Google Patents

METHOD FOR DETERMINING THE PROTEIN CONTENT

Info

Publication number
RU2002119320A
RU2002119320A RU2002119320/13A RU2002119320A RU2002119320A RU 2002119320 A RU2002119320 A RU 2002119320A RU 2002119320/13 A RU2002119320/13 A RU 2002119320/13A RU 2002119320 A RU2002119320 A RU 2002119320A RU 2002119320 A RU2002119320 A RU 2002119320A
Authority
RU
Russia
Prior art keywords
determining
protein content
copper
proteins
potential
Prior art date
Application number
RU2002119320/13A
Other languages
Russian (ru)
Other versions
RU2249818C2 (en
Inventor
Юрий Миронович Волосюк
Денис Владимирович Степанченко
Original Assignee
Донской государственный аграрный университет
Юрий Миронович Волосюк
Денис Владимирович Степанченко
Filing date
Publication date
Application filed by Донской государственный аграрный университет, Юрий Миронович Волосюк, Денис Владимирович Степанченко filed Critical Донской государственный аграрный университет
Priority to RU2002119320/13A priority Critical patent/RU2249818C2/en
Priority claimed from RU2002119320/13A external-priority patent/RU2249818C2/en
Publication of RU2002119320A publication Critical patent/RU2002119320A/en
Application granted granted Critical
Publication of RU2249818C2 publication Critical patent/RU2249818C2/en

Links

Claims (1)

Способ определения содержания белков, включающий создание аналитической среды с электрохимически активными компонентами, введение в нее анализируемого на содержание белков образца, измерение потенциала индикаторного электрода относительно электрода сравнения и определение по величине потенциала количества белков посредством калибровочного графика, отличающийся тем, что в качестве аналитической среды используют щелочной раствор, содержащий ионы гидроксила и ионы меди(II) с массовым отношением 76-119, а измерение потенциала производят на медном индикаторном электроде при массовом отношении меди(II) к белкам 0,156-6,97.A method for determining the protein content, including creating an analytical environment with electrochemically active components, introducing into it a sample analyzed for protein content, measuring the potential of the indicator electrode relative to the reference electrode and determining the value of the amount of proteins by means of a calibration graph, characterized in that the analytical environment is used alkaline solution containing hydroxyl ions and copper (II) ions with a mass ratio of 76-119, and the measurement of the potential on a copper indicator electrode with a mass ratio of copper (II) to proteins of 0.156-6.97.
RU2002119320/13A 2002-07-17 2002-07-17 Protein content determination method RU2249818C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2002119320/13A RU2249818C2 (en) 2002-07-17 2002-07-17 Protein content determination method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2002119320/13A RU2249818C2 (en) 2002-07-17 2002-07-17 Protein content determination method

Publications (2)

Publication Number Publication Date
RU2002119320A true RU2002119320A (en) 2004-10-10
RU2249818C2 RU2249818C2 (en) 2005-04-10

Family

ID=35612047

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2002119320/13A RU2249818C2 (en) 2002-07-17 2002-07-17 Protein content determination method

Country Status (1)

Country Link
RU (1) RU2249818C2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2733935C2 (en) * 2019-03-26 2020-10-08 Федеральное государственное автономное образовательное учреждение высшего образования "Уральский федеральный университет имени первого Президента России Б.Н. Ельцина" Method for targeted covalent immobilisation of proteins on surface of working electrode

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