RO121472B1 - Process for preparing calcium-boron-fructose complex - Google Patents

Abstract

The invention relates to a calcium-boron-fructose complex employed as a nutritional supplement for plants and animals. According to the invention, the process consists in sucessively mixing 4 moles of D-fructose and a natural boron mineral with a content of 2 moles of boron in demineralized water at the temperature of 60A C, and gradually admixing 1 mole of natural calcium carbonate while homogenizing continuously, the resulting solution comprising 6.5 g/l of boron and 17.0 g/l of calcium.

Description

The invention relates to a process for obtaining the calcium-boron-fructose complex, as a solution, used as a nutritional supplement for plants and animals.

Solutions for correcting boron deficiency in plant nutrition are known, by administering boron salts (borates), chemical fertilizers containing potassium and phosphorus containing boron in the soil, by administering organic fertilizers (which can also be done by foliar sprinkling). ). Uses of boron carbohydrate complexes are also known as food supplements for humans, with the use of calcium fructoborate being particularly recommended (PCT / US 98/06050).

Also known are complex food supplements, which also contain a boron compound, for veterinary use, which can also be recommended as a human diet supplement (PCT / NL 92/00015), as well as complex food supplements. containing: calcium, magnesium, silicon, boron and vitamins: A, D and C, created for veterinary use, which is recommended for strengthening bones, cartilages and improving metabolism, especially for racing horses.

The known solutions have the disadvantage that boron-containing food supplements do not cumulate the effect of nutrition with microelements and macroelements and stimulation of metabolic processes.

The technical problem solved by the invention is to determine the optimal working parameters and composition so that the calcium-boron-fructose mixture has properties suitable for use as both a nutritional supplement and a natural stimulant.

The process according to the invention eliminates the mentioned disadvantages, by mixing successively, in demineralized water, at 60 ° C, 4 moles D-fructose and a natural boron mineral, containing 2 moles boron, in which it is added gradually, with continuous homogenization, 1 mol of calcium carbonate of natural origin, the solution obtained having the composition: 6.5 g / l boron and 17.0 g / l calcium, the product being applied foliar in an amount of 5 ... 10 l / ha , as a nutritional supplement for plants, and in an amount of 0.2 ... 4 ml solution / day in drinking water or feed, as a supplement for animal nutrition.

By applying the invention, the following advantages are obtained:

- the use of the product as a nutritional supplement for plants cumulates two effects, one of nutrition with microelements and macroelements (calcium, in significant concentrations), but also a natural stimulating effect of chlorophyll assimilation, which has direct consequences on the efficiency of the nutrition of the plant;

- by using in the feeding of animals and birds in the youth phase and mineral-vitamin deficiencies, the following effects are obtained:

- a significant strengthening of the bones, because it stimulates the absorption and retention of calcium and magnesium and stimulates the metabolic processes that provide energy, and in particular glycolysis and the Krebs cycle, with beneficial effects in osteoporosis, osteoarthritis and rheumatoid arthritis;

- increases the body's immunity and increases its resistance to diseases;

- ensures a significant increase in the assimilation of vitamins and minerals from food;

- it improves the productive performances (the increase parameters);

- ensures a significant increase in the assimilation of vitamins and minerals from food;

- improves biochemical parameters (lowers cholesterol, increases fat quality by increasing the ratio between saturated and unsaturated fatty acids);

- increases the concentration of calcium in the serum, avoiding unwanted phenomena before and after calving;

- favors the deposition of calcium in the egg shell and improves the percentage of hatching and vitality of the chickens;

- decreases the percentage of mortality;

- causes changes in the composition of the meat (cholesterol, saturated acids / unsaturated acids ratio);

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- in antistress; after vaccination; in convalescent states.

The product created cumulates two effects, one of nutrition with microelements and macroelements (calcium, in significant concentrations), but also a natural stimulating effect of chlorophyll assimilation, which has direct consequences on the efficiency of plant nutrition.

The calcium-boron-fructose complex according to the present invention can be prepared from natural minerals containing boron (Tinai, Sassolit, Colemanite, Kernite, Ulexite, Hydroxyboracite) fructose and calcium carbonate of natural origin (Crete, Calcit, Dolomite, Coral) .

The solution obtained has the following characteristics: colorless liquid, with a slight opalescent tint; density (kg / dmc): 1,170 ... 1,180; pH (concentrated solution): 5.0 ... 5.5; pH (dilute solution; 1:15 dilution; 1: 100) 7.0 ... 7.5; composition: bor (B) (g / l): minimum 6.5; calcium (Ca) (g / l): minimum 17.0.

The process for obtaining the calcium-boron-fructose complex according to the invention consists of the following steps: dosing of the raw materials, according to the manufacturing recipe, introduction of demineralized water in the reactor, previously heated to 60 ° C; introduction of fructose, homogenization of the mixture until complete dissolution, introduction of natural mineral containing boron, homogenization of the mixture until complete dissolution, addition of calcium carbonate of natural origin, in small quantities, gradually, concomitantly with the homogenization of the mixture, following the completion of the release CO ₂ , filtration of the obtained solution, packaging and labeling.

The following is an example of an embodiment of the invention.

Example. The composition for use as a fertilizer and stimulant for plants, as well as the composition for use as a supplement for animal nutrition is prepared by mixing in water at 60'0 4 moles D-fructose (4 moles) with a natural mineral of boron (2 moles of boron), after which the calcium carbonate of natural origin (1 mol) is introduced, gradually, following the completion of the release of CO ₂ , finally, the obtained solution is filtered and packaged.

For use as a nutritional supplement for plants (foliar stimulator), the product is applied in the amount of 5 ... 10 l / ha, by foliar spray, depending on the crop to which it is applied.

For use as an animal nutrition supplement, the product is applied by dilution in feed or drinking water, the dose being the following:

- for birds: the dose is 0.2 ... 0.4 ml calcium-boron-fructose / day complex solution;

- for animals: the dose is 2 ... 4 ml calcium-boron-fructose / day complex solution. The results of the use of the complex, according to the invention, are shown in table 1 and in annexes 1 ... 8.

Table 1

Use of the calcium-boron-fructose complex as foliar fertistimulator (doses and time of application)

Nr. books. Culture Nr. sprinkler Spray dose (l / ha) / Total dose (l / ha) Time of application 1. tomatoes 2 5/10 - in seedlings, when the plants have 3 - 4 true leaves (2 applications can be made at an interval of 7 -10 days) - the vegetation period (2 weeks after planting) (2 applications can be made at intervals of 10 - 15 days)

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Table 1 (continued)

Nr. crt. Culture Nr. sprinkler Spray dose (l / ha) / Total dose (l / ha) Time of application 2. Pepper 2 5/10 - in seedlings, when the plants have 3 - 4 true leaves (2 applications can be made at an interval of 7 -10 days) - the vegetation period (2-3 weeks after planting) at intervals of 10-15 days 3. Eggplants 2 5/10 - in seedlings, when the plants have 3 - 4 true leaves (2 applications can be made at an interval of 7 -10 days) - the vegetation period (2-3 weeks after planting) at intervals of 10-15 days 4. Cabbage 2 5/10 - in seedlings, when the plants have 2 true leaves (2 applications can be made at a range of 7 -10 days) - the vegetation period (3 to 4 weeks after planting, and at the beginning of the master's formation) 5. Cucumbers 2 5/10 - the vegetation period (when the first 3 - 5 true leaves appear, when the plant is 20 - 25 cm high), at intervals of 10-15 days 6. Sunflower 1 ΤΠ - when the plants are 40 - 50 cm (at 4-5 true leaves) 7. Pile cereals (wheat, barley, barley, rye) 1 7/7 - spring, after starting in vegetation, when the plant is 20 - 25 cm, until the emergence of the spike 8. Corn 1 7/7 - after starting in vegetation, when 6 - 7 true leaves appear, when the plant is 30 - 40 cm 9. Potato 2 5/10 - after starting in vegetation, when the plant is 25 - 30 cm, before and after flowering 10. Tobacco 1 7/7 - in vegetation, when the plant is 15 20 cm

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Table 1 (continued)

Nr. books. Culture Nr. sprinkler Spray dose (l / ha) / Total dose (l / ha) Time of application 11. Vine 2 5/10 - at the advanced formation of the leaves, when the shoots have a length of 20 25 cm; at intervals of 10-15 days 12. Fruit trees 1 7/7 - after the formation of the leaf system (leaves) 13. Lucerne and lawn 2-3 5/15 - spring, when the plant is 8 - 10 cm; and after each mowing 14. Flowers (field culture) 2 5/10 - at the occurrence of 3 to 5 leaves; at intervals of 20 - 25 days

Appendix 1

Testing the calcium-boron-fructose complex as a foliar fertilizer

Species: Sunflower (Helianthus annuus L.)

The simple hybrid: Fundulea 206

Dose: 1% with 500 I solution / ha, or 5 I complex / ha

Application time: 4 - 5 true leaves phase - the appearance of the floral bud

Number of applications: two

Application interval: 10-15 days

Experimental variants: 2

V, = control sprinkled with water;

V ₂ = complex (1% with 500 I solution / ha).

The experience was located on a soil cernoziom cambio, without basic fertilization.

Production results (kg / ha) obtained from the sunflower culture, the simple hybrid "Fundulea 206 at S.C.D.A. Caracal - Olt in 2002

Cousin. Specification Number of treatments Conc. solution % Amount of complex used l / ha Seed production The increase achieved to a treatment The total kg / ha % kg / l com- plexus 1 MT sprinkled with water - - - - 1220 - 100.0 - 2 Ca-Bor-Fru complex 2 1, o 5 10 1480 ** 260 121, 3 26

DL 5% = 170

DL 1% = 230

DL 0.1% = 280

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Annex 2

Testing of the calcium-boron-fructose complex as foliar fertilizer Species: Tomatoes (Lycopersicon lycopersicum (L.) Karst ex. Farw. Variety: Romec 554 J

Dose: 1% with 500 I solution / ha, or 5 I Standard Folibor / ha

Application time: first application at 7-10 days after planting

Number of applications: two

Application interval: 10-75 days

Experimental variants: 2

V, = control sprinkled with water;

V ₂ = complex (1% with 500 I solution / ha).

The experience was located on an alluvial soil with a neutral to weak alkaline reaction (pH = 7.0 ... 7.4) and a nutrient content of:

NNO ₃ = 59.5 - 73.5 ppm;

P _AL = 119-220 ppm;

K _AL = 291 - 307 ppm.

The production results (kg / ha) obtained from the crop of summer-autumn tomatoes, the variety "Romec 554 J at S.C.D.L. Işalnita in 2002

Cousin. Specification Number of treatments Conc. solution % Amount of complex used l / ha Absolute production The increase achieved to a treatment The total kg / ha % kg / l complex 1 MT sprinkled with water - - - - 44300 - 100.0 - 2 Ca-Bor-Fru complex 2 1.0 5 10 53800 ** 9500 121.4 950

DL 5% = 3742

DL 1% = 6192

DL 0.1% = 11589

Annex 3

Testing of the calcium-boron-fructose complex as foliar fertilizer Species: Bell peppers (Capsicum annuumn L. ssp annuum var. Grossum) Variety: Ișalnița 85 V

Dose: 1% with 500 I solution / ha, or 5 I Complex / ha

Time of application: first application at 7 ... 10 days after planting Number of applications: two

Application interval: 10 ... 15 days

Experimental variants: 2

V, = control sprinkled with water

V ₂ = complex (1% with 500 I solution / ha).

The experience was located on an alluvial soil with a neutral to weak alkaline reaction (pH = 7.0 ... 7.4) and a nutrient content of:

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NNO, = 59.5- 73.5 ppm;

P _AL = 119-220 ppm;

K _AL = 291 - 307 ppm.

Production results (kg / ha) obtained from the cultivation of peppers, variety "Ișalnița 85 V at S.C.D.L. Işalnița in 2002

Cousin. Specification Number of treatments Conc. solution% Amount of complex used l / ha Seed production The increase achieved to a treatment The total kg / ha % kg / l complex 1 MT sprinkled with water - - - - 24600 - 100.0 - 2 Ca-Bor-Fru complex 2 1.0 5 10 35000 ** 11400 145.1 1140

DL 5% = 4915 DL1% = 8133

DL 0.1% = 15222

Annex 4

Testing the calcium-boron-fructose complex as a foliar fertilizer

Species: Eggplants (Solanum melongena L.)

Variety: Lucia

Dose: 1% with 500 I solution / ha, or 5 I complex / ha

Application time: first application at 7-10 days after planting

Number of applications: two

Application interval: 10-15 days

Experimental variants: 2

Vi = control sprinkled with water;

V ₂ = Complex (1% with 500 I solution / ha).

The experience was located in an alluvial soil with a neutral to weak alkaline reaction (pH = 7.0 - 7.4) and a nutrient content of:

NNO ₃ = 59.5 -73.5 ppm;

P _AL = 119 - 220 ppm;

K _AL = 291 - 307 ppm.

The production results (kg / ha) obtained at the eggplant crop, the variety "Lucia at S.C.D.L. Işalnița in 2002

Cousin. Specification Number of treatments Conc. solution% Amount of complex used l / ha Absolute seed production The increase achieved to a treatment The total kg / ha % kg / l complex 1 MT sprinkled with water - - - - 36400 - 100.0 - 2 Ca-Bor-Fru complex 2 1.0 5 10 48200 ** 11800 132.3 1180

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DL 5% = 2747

DL 1% = 4545

DL 0.1% = 8507 ***

Annex 5

Testing the calcium-boron-fructose complex as a foliar fertilizer

Species: Autumn wheat (Triticum aestivum L.)

Variety. bustard

Dose: 1% with 500 I solution / ha, or 5 I complex / ha

Application time: spring when the plants are 10-15 cm high

Number of applications: two

Application interval: 10-15 days

Experimental variants: 2

V ₁ = control sprayed with water;

V ₂ = Complex (1% with 500 I solution / ha).

The experience was located on an alluvial soil with a neutral to weak alkaline reaction (pH = 7.0 ... 7.4) and a nutrient content of:

NNO ₃ = 59.5 - 73.5 ppm,

P _AL = 119 - 220 ppm;

K _AL = 291 - 307 ppm.

Production results (kg / ha) obtained at the autumn wheat crop, the variety "Dropia at S.C.D.L. Işalnița in 2002

Cousin. Specification Number of treatments Conc. solution% Amount of complex used l / ha Absolute production kg / ha The increase achieved to a treatment The total kg / ha % kg / l complex 1 MT sprinkled with water - - - - 2000 - 100.0 - 2 Ca-Bor-Fru complex 2 1.0 5 10 2540 ** 540 127.0 54

DL 5% = 322

DL1% = 522

DL 0.1% = 998 ***

Annex 6

Testing the calcium-boron-fructose complex as a foliar fertilizer

Species: Tobacco (Nicotiana tabacum L.)

Variety: Victoria

Dose: 1 /% with 500 I solution / ha, or 5 I complex / ha

Time of application: first application at 7 - 10 days after planting

Number of applications: two

Application interval: 10-15 days

Experimental variants: 2

V, = control sprinkled with water;

V ₂ = Complex (1% with 500 I solution / ha).

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The experience was located at the experimental base CHIMENERG Ișalnița - Dolj.

Production results (kg / ha) obtained from tobacco cultivation, variety "Virginia, at CHIMENERG Ișalnița in 2002

Cousin. Specification Number of treatments Conc. solution% Amount of complex used l / ha Production of dried leaves kg / ha The increase achieved to a treatment The total kg / ha % kg / l complex 1 MT sprinkled with water - - - - 1934 - 100.0 - 2 Ca-Bor-Fru complex 2 1.0 5 10 2152 ** 218 111.3 21.8

DL 5% = 165

DL 1% = 231

DL 0.1% = 310

Appendix 7 Testing the calcium-boron-fructose complex as a nutritional supplement for broiler chickens ♦ Control group (M) ♦ Experimental group 1 (E1) - was fed with the same nutrients combined as group M, but every day an im was added volume of complex to provide a tested product intake of 0.3337g / kg chicken (recommended by the manufacturer).

♦ Experimental lot 2 (E2) - was fed with the same feeds combined as group M, but each day a volume of complex was added which would provide a double contribution to group E1.

Bioproductive performance (cumulative data during the 0-42 days experience)

UM M E1 E2 Initial weight g / c 34.09 32.56 32.04 Final weight g / c 1862.47 1857.98 1898.68 Daily average consumption g / chicken / day 80.95 79.54 79.61 I increase daily average g / chicken / day 43.64 43.46 44,45 Specific consume g / g 1.85 1.83 1.79 ^inhab

Where a = significantly different (p <0.05) from M b = significantly different (p <, 0.05) from E1 c = significantly different (p <0.05) from E2

Chemical analysis of bones - 28 days

leg bone witness Exp.1 Exp.2 Dry substance 105 ° C (g%) 41.75 ± 1,220 44.27 ± 1.057 42.00 ± 2.152 Gross fat (g%) 4.06 ± 0.577 4.99 ± 0.979 4.28 ± 0.777

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Table (continued)

leg bone witness Exp.1 Exp.2 Ash (g%) 52.90 ± 0.165 53.78 ± 0.607 53.40 ± 1.289 Calcium (g%) 28.11 ± 0.149 28.45 ± 0.317 28.22 ± 0.179 Phosphorus (g%) 14.07 ± 0.070 14.23 ± 0.236 13.37 ± 0.326 Boron (mg%) 0.01 ± 0.0016 0.02 ^ac ± 0.0017 0.027 ^ab ± 0.0016

FEMUR witness Exp. 1 Exp. 2 Dry substance 105 uC (g%) 44.86 ± 1.135 46.14 ± 0.245 46.12 ± 0.914 Gross fat (g%) 6.70 ± 1.469 7.43 ± 2.097 5.27 ± 0.906 Ash (g%) 51.74 ± 0.711 52.90 ± 0.676 52.90 ± 0.757 Calcium (g%) 27.88 ± 0.123 27.60 ± 0.209 27.31 ± 0.295 Phosphorus (g%) 14.78 ± 0.117 13.89 ^to ± 0.073 13.92 ^to ± 0.032 Boron (mg%) 0.01 ± 0.000 0.03 ^to ± 0.005 0.029 ^to ± 0.001

a = significantly different (p _s 0.05) from M b = significantly different (p <0.05) from E1 c = significantly different (p _s 0.05) from E2

Appendix 8 Testing the calcium-boron-fructose complex as a nutritional supplement in the feeding of piglets after weaning

The rations were isoproteic and isocaloric for the control and experimental groups, the difference between the rations consisting of the addition of the complex (liquid product) administered to the experimental group.

Productive performance (average values / lot)

Specification Witness batch Experimental lot Enhancement parameters: - final weight, kg / pig - increase weight, kg / pig - daily average gain, kg / day / pig - average daily consumption, kg / day / pig - specific consumption (kg / kg) 38.12 ± 1.886 26.38 ± 1.886 0.525 ± 0.037 1.469 ± 0.075 2.798 ± 0.149 38.75 ± 0.829 27,375 ± 0.718 0.547 ± 0.014 1,311 ± 0.023 2.396 ± 0.044 Development parameters: - carcass, kg / pig - head, kg / pig - tabs, kg / pig - heart, kg / pig - liver, kg / pig - lung, kg / pig 28.250 2.65 ± 0.05 0.685 ± 0.005 0.145 ± 0.005 0.880 ± 0.03 0.470 ± 0.00 29.125 2.97 ± 0.184 0.730 ± 0.014 0.130 ± 0.014 0.915 ± 0.021 0.420 ± 0.014 Yield at slaughter,% 70.63 71.03

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Crude chemical composition in muscle and pulp * 1

Specification Real dry matter % Crude protein % Raw fat % Ash % MUSCLE witness 27.66 23.12 3.28 1.20 Experimental 26.08 22.86 2.19 1.12 PULP witness 26.54 22.89 2.48 1.15 Experimental 25.42 22,12 2.14 1.12

'The results are expressed in% sample as such. 9

Claims (2)

1. Process for obtaining the calcium-boron-fructose complex as a solution, 13 used as a nutrition supplement for plants and animals, characterized by successively mixing in demineralized water at 60 ° C, 4 moles D- fructose and a 15 natural boron mineral, containing 2 moles boron, to which 1 mol of calcium carbonate of natural origin is added gradually, with the solution obtained having in 17 composition 6.5 g / l boron and 17 , 0 g / l calcium. 2. Method of applying the calcium-boron-fructose complex, defined in claim 1, 19, characterized in that the product is applied foliar, in an amount of 5 ... 10 l / ha, as a nutritional supplement for plants, and in quantity. 0,2 ... 4 ml solution / day in drinking water or feed, 21 as an animal nutrition supplement.