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PT2990478T - Luciferases de oplophorus sintéticas com débito luminoso aumentado - Google Patents

Luciferases de oplophorus sintéticas com débito luminoso aumentado

Info

Publication number
PT2990478T
PT2990478T PT151866522T PT15186652T PT2990478T PT 2990478 T PT2990478 T PT 2990478T PT 151866522 T PT151866522 T PT 151866522T PT 15186652 T PT15186652 T PT 15186652T PT 2990478 T PT2990478 T PT 2990478T
Authority
PT
Portugal
Prior art keywords
light output
enhanced light
oplophorus luciferases
synthetic oplophorus
synthetic
Prior art date
Application number
PT151866522T
Other languages
English (en)
Inventor
P Encell Lance
V Wood Keith
G Wood Monika
Hall Mary
Otto Paul
Vidugiris Gediminas
Zimmerman Kristopher
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of PT2990478T publication Critical patent/PT2990478T/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y113/00Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/61Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PT151866522T 2009-05-01 2010-05-03 Luciferases de oplophorus sintéticas com débito luminoso aumentado PT2990478T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US17483809P 2009-05-01 2009-05-01

Publications (1)

Publication Number Publication Date
PT2990478T true PT2990478T (pt) 2017-05-08

Family

ID=42358981

Family Applications (4)

Application Number Title Priority Date Filing Date
PT181846999T PT3409764T (pt) 2009-05-01 2010-05-03 Luciferases de oplophorus sintéticas com débito luminoso aumentado
PT17154777T PT3181687T (pt) 2009-05-01 2010-05-03 Luciferases de oplophorus sintéticas com débito luminoso aumentado
PT151866522T PT2990478T (pt) 2009-05-01 2010-05-03 Luciferases de oplophorus sintéticas com débito luminoso aumentado
PT107170136T PT2456864E (pt) 2009-05-01 2010-05-03 Luciferases de oplophorus sintéticas com débito luminoso aumentado

Family Applications Before (2)

Application Number Title Priority Date Filing Date
PT181846999T PT3409764T (pt) 2009-05-01 2010-05-03 Luciferases de oplophorus sintéticas com débito luminoso aumentado
PT17154777T PT3181687T (pt) 2009-05-01 2010-05-03 Luciferases de oplophorus sintéticas com débito luminoso aumentado

Family Applications After (1)

Application Number Title Priority Date Filing Date
PT107170136T PT2456864E (pt) 2009-05-01 2010-05-03 Luciferases de oplophorus sintéticas com débito luminoso aumentado

Country Status (16)

Country Link
US (7) US8557970B2 (pt)
EP (5) EP3409764B1 (pt)
JP (2) JP6038649B2 (pt)
KR (1) KR101665352B1 (pt)
CN (2) CN108192950B (pt)
AU (1) AU2010242771B2 (pt)
BR (1) BRPI1010019B1 (pt)
CA (1) CA2758572A1 (pt)
DK (4) DK2456864T3 (pt)
ES (4) ES2691539T3 (pt)
IL (3) IL215697B (pt)
LT (1) LT3409764T (pt)
PL (4) PL2990478T3 (pt)
PT (4) PT3409764T (pt)
SG (3) SG10201601281WA (pt)
WO (1) WO2010127368A1 (pt)

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Also Published As

Publication number Publication date
EP3181687B1 (en) 2018-08-08
JP2016028599A (ja) 2016-03-03
DK3409764T3 (da) 2020-05-25
DK2456864T3 (en) 2015-12-21
KR101665352B1 (ko) 2016-10-12
US10233485B2 (en) 2019-03-19
US20100281552A1 (en) 2010-11-04
IL274145A (en) 2020-06-30
JP6038649B2 (ja) 2016-12-07
DK2990478T3 (en) 2017-05-15
IL265263B (en) 2020-05-31
EP2990478B1 (en) 2017-04-05
EP3409764B1 (en) 2020-04-15
CA2758572A1 (en) 2010-11-04
WO2010127368A1 (en) 2010-11-04
US20190185908A1 (en) 2019-06-20
US9777311B2 (en) 2017-10-03
PT3181687T (pt) 2018-10-19
PT3409764T (pt) 2020-05-29
PL3409764T3 (pl) 2020-07-13
US20210032676A1 (en) 2021-02-04
EP3744834A1 (en) 2020-12-02
US20200248228A1 (en) 2020-08-06
JP6227615B2 (ja) 2017-11-08
CN108192950B (zh) 2021-09-03
ES2629390T3 (es) 2017-08-09
PL2456864T3 (pl) 2016-04-29
SG10201601281WA (en) 2016-03-30
EP3181687A1 (en) 2017-06-21
US8557970B2 (en) 2013-10-15
EP3409764A1 (en) 2018-12-05
LT3409764T (lt) 2020-06-10
EP2990478A1 (en) 2016-03-02
PL2990478T3 (pl) 2017-08-31
CN108192950A (zh) 2018-06-22
PT2456864E (pt) 2016-01-20
ES2691539T3 (es) 2018-11-27
IL215697A0 (en) 2012-01-31
US10633690B2 (en) 2020-04-28
US20220389434A1 (en) 2022-12-08
EP2456864B1 (en) 2015-11-11
IL215697B (en) 2019-03-31
US11667950B2 (en) 2023-06-06
US11365436B2 (en) 2022-06-21
AU2010242771A1 (en) 2011-11-03
KR20120088548A (ko) 2012-08-08
PL3181687T3 (pl) 2018-11-30
IL265263A (en) 2019-05-30
IL274145B (en) 2021-07-29
CN102459579B (zh) 2018-02-09
AU2010242771B2 (en) 2015-01-22
SG10201901453PA (en) 2019-03-28
CN102459579A (zh) 2012-05-16
EP2456864A1 (en) 2012-05-30
JP2012525819A (ja) 2012-10-25
DK3181687T3 (en) 2018-09-10
US20180051318A1 (en) 2018-02-22
SG175180A1 (en) 2011-11-28
ES2795287T3 (es) 2020-11-23
US20140120548A1 (en) 2014-05-01
BRPI1010019A2 (pt) 2015-08-25
BRPI1010019B1 (pt) 2019-02-05
ES2559505T3 (es) 2016-02-12
US10844422B2 (en) 2020-11-24

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