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PT1078051E - Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas - Google Patents

Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas Download PDF

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Publication number
PT1078051E
PT1078051E PT99922306T PT99922306T PT1078051E PT 1078051 E PT1078051 E PT 1078051E PT 99922306 T PT99922306 T PT 99922306T PT 99922306 T PT99922306 T PT 99922306T PT 1078051 E PT1078051 E PT 1078051E
Authority
PT
Portugal
Prior art keywords
virus
selection
polypeptide
phage
display system
Prior art date
Application number
PT99922306T
Other languages
English (en)
Inventor
Lutz Riechmann
Peter Kristensen
Jean-Luc Jestin
Gregory Paul Winter
Original Assignee
Domantis Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9810228.8A external-priority patent/GB9810228D0/en
Priority claimed from GBGB9810223.9A external-priority patent/GB9810223D0/en
Application filed by Domantis Ltd filed Critical Domantis Ltd
Publication of PT1078051E publication Critical patent/PT1078051E/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Control Of Indicators Other Than Cathode Ray Tubes (AREA)
  • Saccharide Compounds (AREA)
  • Vehicle Body Suspensions (AREA)
  • Circuits Of Receivers In General (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Air Bags (AREA)
PT99922306T 1998-05-13 1999-05-13 Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas PT1078051E (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9810228.8A GB9810228D0 (en) 1998-05-13 1998-05-13 Selection method
GBGB9810223.9A GB9810223D0 (en) 1998-05-13 1998-05-13 Selection system

Publications (1)

Publication Number Publication Date
PT1078051E true PT1078051E (pt) 2008-03-17

Family

ID=26313668

Family Applications (1)

Application Number Title Priority Date Filing Date
PT99922306T PT1078051E (pt) 1998-05-13 1999-05-13 Sistema de apresentação em fagos para a selecção de proteínas correctamente organizadas

Country Status (14)

Country Link
US (2) US7442159B1 (pt)
EP (2) EP1950295A1 (pt)
JP (2) JP4493846B2 (pt)
AT (1) ATE380865T1 (pt)
AU (1) AU762814B2 (pt)
CA (1) CA2328422A1 (pt)
CY (1) CY1107293T1 (pt)
DE (1) DE69937735T2 (pt)
DK (1) DK1078051T3 (pt)
ES (1) ES2299244T3 (pt)
GB (1) GB2361237A (pt)
NO (1) NO329172B1 (pt)
PT (1) PT1078051E (pt)
WO (1) WO1999058655A2 (pt)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1100890A2 (en) 1998-07-27 2001-05-23 Genentech, Inc. Improved transformation efficiency in phage display through modification of a coat protein
DE60041119D1 (de) 1999-07-20 2009-01-29 Morphosys Ag Verfahren zur präsentation von (poly)peptiden/proteinen auf bakteriophagenpartikeln via disulfidbindungen
JP2003523742A (ja) * 2000-02-03 2003-08-12 ドマンティス リミテッド コンビナトリアルタンパク質ドメイン
GB0019049D0 (en) * 2000-08-04 2000-09-27 Neutec Pharma Plc Combinatorial chemistry method
WO2002012277A2 (en) * 2000-08-07 2002-02-14 Domantis Limited Hybrid combinatorial proteins made from reshuffling of differently folded domains
DE10135039C1 (de) * 2001-07-18 2003-03-13 Nemod Immuntherapie Ag Verfahren zur Isolierung großer Varianzen spezifischer Moleküle für ein Zielmolekül aus Phagemid-Gen-Bibliotheken
EP2357237A1 (en) 2003-05-14 2011-08-17 Domantis Limited A process for recovering polypeptides that unfold reversibly from a polypeptide repertoire
EP1902131B1 (en) 2005-07-08 2009-11-11 University of Zurich Phage display using cotranslational translocation of fusion polypeptides
GB2428293A (en) * 2005-07-13 2007-01-24 Domantis Ltd Phage display libraries
ATE524579T1 (de) 2005-07-21 2011-09-15 Aebi Ltd Verfahren und zusammensetzungen zur identifizierung eines peptids mit intermolekularer wechselwirkung mit einem interessierenden ziel
GB0515115D0 (en) 2005-07-22 2005-08-31 Isogenica Ltd Peptide characterisation
JP5564249B2 (ja) * 2006-06-23 2014-07-30 アレシア・バイオセラピューティクス・インコーポレーテッド 癌に関与するポリヌクレオチド配列およびポリペプチド配列
CA2688434A1 (en) 2007-06-06 2008-12-11 Domantis Limited Polypeptides, antibody variable domains and antagonists
US9062097B2 (en) 2007-08-21 2015-06-23 Morpho Sys AG Methods for the formation of disulphide bonds
UY31504A1 (es) 2007-11-30 2009-07-17 Construcciones de union de antigenos
CN104829714A (zh) 2008-11-03 2015-08-12 阿莱斯亚生物疗法股份有限公司 特异性地阻滞肿瘤抗原的生物活性的抗体
ES2688621T3 (es) 2008-12-05 2018-11-05 Glaxo Group Limited Métodos para seleccionar polipéptidos resistentes a proteasa
EP2401298A1 (en) 2009-02-24 2012-01-04 Glaxo Group Limited Antigen-binding constructs
US20110305694A1 (en) 2009-02-24 2011-12-15 Paul Andrew Hamblin Multivalent and/or multispecific rankl-binding constructs
JP2012518399A (ja) 2009-02-24 2012-08-16 グラクソ グループ リミテッド 抗原結合性構築物
EP2643351A1 (en) 2010-11-24 2013-10-02 Glaxo Group Limited Multispecific antigen binding proteins targeting hgf
RS59080B1 (sr) 2011-03-31 2019-09-30 Adc Therapeutics Sa Antitela protiv antigena 1 povezanog sa bubrezima i njihovi fragmenti koji se vezuju za antigen
CA2842099A1 (en) 2011-07-27 2013-01-31 Glaxo Group Limited Antigen binding constructs
EP2771456B1 (en) * 2011-10-27 2018-08-15 The Governors of the University of Alberta Device and method for bacterial culture and assay
LT2802351T (lt) 2012-01-09 2019-06-25 Adc Therapeutics Sa Agentai, skirti trigubai neigiamo krūties vėžio gydymui
CA3214529A1 (en) * 2013-09-25 2015-04-02 Cytomx Therapeutics, Inc. Matrix metalloproteinase substrates and other cleavable moieties and methods of use thereof
EP3099319A2 (en) 2014-01-31 2016-12-07 Cytomx Therapeutics Inc. Matriptase and u-plasminogen activator substrates and other cleavable moieties and methods of use thereof
WO2016074683A1 (en) * 2014-11-11 2016-05-19 Lundorf Pedersen Materials Aps Method for identification of molecules with desired characteristics
IL300408B2 (en) 2015-01-20 2025-11-01 Cytomx Therapeutics Inc Matrix metalloprotease-cleavable and serine protease cleavable substrates and methods of use thereof
EP3277713B1 (en) 2015-03-31 2025-09-10 Sorriso Pharmaceuticals, Inc. Polypeptides
CA2981098A1 (en) * 2015-03-31 2016-10-06 Vhsquared Limited Peptide construct having a protease-cleavable linker
EP3827116A4 (en) 2018-07-23 2022-05-11 The Governors of the University of Alberta Genetically-encoded bicyclic peptide libraries
MX2021006429A (es) 2018-12-06 2021-07-15 Cytomx Therapeutics Inc Sustratos escindibles con matriz de metaloproteasa, serina o cisteína escindible con proteasa y métodos de uso de estos.
CN114466864B (zh) 2019-06-21 2024-12-27 索瑞索制药公司 多肽
CN114008195A (zh) * 2019-06-21 2022-02-01 勃林格殷格翰国际有限公司 控制rna病毒复制和基因表达的新机制

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
ES2052027T5 (es) 1988-11-11 2005-04-16 Medical Research Council Clonacion de secuencias de dominio variable de inmunoglobulina.
CA2016842A1 (en) 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
US5723286A (en) * 1990-06-20 1998-03-03 Affymax Technologies N.V. Peptide library and screening systems
GB9015198D0 (en) * 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
EP0585287B1 (en) * 1990-07-10 1999-10-13 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
ES2330052T3 (es) * 1991-03-01 2009-12-03 Dyax Corporation Proteina quimerica que comprende micro-proteinas que tienen dos o mas puentes disulfuro y relaizaciones de las mismas.
DE4122599C2 (de) * 1991-07-08 1993-11-11 Deutsches Krebsforsch Phagemid zum Screenen von Antikörpern
ES2341666T3 (es) 1991-12-02 2010-06-24 Medimmune Limited Produccion de autoanticuerpos de repertorios de segmentos de anticue rpos expresados en la superficie de fagos.

Also Published As

Publication number Publication date
NO329172B1 (no) 2010-09-06
ATE380865T1 (de) 2007-12-15
AU3941399A (en) 1999-11-29
US8680018B2 (en) 2014-03-25
WO1999058655A2 (en) 1999-11-18
US20090203542A1 (en) 2009-08-13
DE69937735T2 (de) 2008-11-27
DE69937735D1 (de) 2008-01-24
AU762814B2 (en) 2003-07-03
CY1107293T1 (el) 2012-11-21
GB2361237A (en) 2001-10-17
EP1078051B1 (en) 2007-12-12
GB0026143D0 (en) 2000-12-13
JP4493846B2 (ja) 2010-06-30
ES2299244T3 (es) 2008-05-16
NO20005628L (no) 2001-01-12
JP2010148507A (ja) 2010-07-08
WO1999058655A3 (en) 2000-01-27
EP1950295A1 (en) 2008-07-30
EP1078051A2 (en) 2001-02-28
CA2328422A1 (en) 1999-11-18
DK1078051T3 (da) 2008-04-07
JP5161865B2 (ja) 2013-03-13
JP2002514413A (ja) 2002-05-21
US7442159B1 (en) 2008-10-28
NO20005628D0 (no) 2000-11-08

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