PT106071B - THYMBRA ESSENTIAL OIL AND CHITOSAN GEL CAPITATA FOR THE TREATMENT OF VULOVOVAGINAL CANDIDOSE - Google Patents

Abstract

This invention relates to a gel resulting from the association of chymosan and essential oil of THYMBRA CAPITATA with a view to its use in the treatment of vulvo-vaginal candidiasis. The chitosan and essential oils of TIMBRA represent an innovative approach, since the market already exists in the market. SOME PHARMACEUTICAL FORMULATIONS BASED ON NATURAL PRODUCTS OF VAGINAL APPLICATION BUT THAT ARE IN THEIR OWN CREMES. THIS GEL ASSOCIATES TWO NATURAL PRODUCTS WITH A SUCCESSFUL ANTI-CANDIDA EFFECT, QUITOSAN AND ESSENTIAL OIL, BEING INNOVATIVE AT THE LEVEL OF ITS FORMULATION, TECHNOLOGY AND USES. The present invention has application in the treatment of vulvar vaginal candidiasis which is one of the most common feminine infections, affecting approximately 70-75% of the female population at least once in life.

Description

The emergentincidence of beginning and endangels, and the development of resistance to the classical agents, a challenge to the scientist. Accordingly, the therapeutic alternative processor, such as natural prodrugs, toas and other essential oils, is the essential oil of Thymbx: the capitata is traditionally considered a potent antiseismic agent. in the treatment of catheter infections. We further describe the amino acid effect of this product on pianethogenic cells, reported here only; primary heat-mediated fnngi.ci. effect; ostcp membrane; asthma, asthma, the ability to inhibit the formation of all germination (willow, L. R <? sinto, E <; Gonçalves, R, Ji; P Loa - Vas, Cd; Cavaleiro, C ..; (A, Costa de Oliveira, Martins, Oliveira, J., Chemical Compounding and Anti-Phial Activation of the Essential Oxygen of Thymbra. capitata * Plant dedicated, 2004, 70, (0), 512-515}.

Also, for this natural product, we have also found the interesting and well-known antigen in pre-formed biofilms of this yeast i Pomerase-1, A.g Gaspar, (A;

Ra ime ?. gt; < / RTI > -a, R.? Silva-Dias, A.G. et al., C .; Cavalssi.ro, C .; Pina • tas, C.; bart is m-d © -Oliveira, J .; Q'.se. > .ror, o. THE,; Rodrignes, Av., Et al., On the activity of Tissues et al., Et al., On the basis of biophysical development, J. Biochem., 2012, JDD, 2), 32, 303).

Chitosan is, as a result, highly active. In the present invention, the invention is directed to the species Danai and of limited tonicity in homase cells (Raimeira-de-OAiveira, A, Ribeiro, iUP, Raimeira-de-Giiveira, R, Caspar, Qiineira, et al., Proc., Pina Vac, C ..; Baxter, D.-Giviteira, et al., Proctor, J.Ag Rodrigues, A> Gv, Anti-Candida activlty © fa ohitoaan hydrogel : mechanism of action and cytotox.ici.tv profiie · dynoooi bestei ον © η η, 30X0, 20, (i) 322-327: Furlmeira-ftBmb; veira, 2;

Gaspar .. CP; Raimei et al. Sarmento, B .; Cíartixiea-ae " Oliveira, O ,; Fina- Vare CP; Rodrigues, A, G,; Queiror, 0 ', A., Ode relationsMp hetvoen Candida species cAurge cieoui ty and. ctm.tosan actdvi ty evalaated by ion ~ exctange cnr orna s ogr aphy. A method for the preparation of a pharmaceutical composition according to any of the preceding claims, characterized in that the composition of the present invention comprises the steps of: It is true that most of the pharmaceutical formulations available in the sero-type have a rheological and adhesion-specific character which are not very suitable for the valid application. The preparations have traditionally been commercialized, and the creams, foams, gels, irrigations and tablets have been shown to have a short retention time, due to the vaginal autodepuration mechanism, causing discomfort and soreaction of eacorreunia, absorption kinetics and broatr vi. of the vaginal formulations depends on their interaction with the fluids; vaginal residents. Vaginal drug delivery benefits from the use of bi-lingual systems. Vaginal application formulations of the gel are available in the pharmaceutical market. Most of these products are sewn together as lubricants, not by any agent However, the vaginal application of the acids with the purpose of restoring the pH: vaginal acid, in the therapeutic spremeurarity applicable to various vaginal intentions are also available, alternative therapeutic proposals are constituted by the base of the gol without therapeutic effect and conveying to the classic nivcinrerobian.os compositions, somi-solid formulations conveying natural antimicrobial compounds for vaginal aphcation are generally available. According to the invention, the invention relates to a composition according to the invention in which the composition according to the invention is characterized in that it comprises a lactate, the present invention differs from that patent to the extent that its final composition and effects produced on the basis of this technology are demarcated therefrom. The present invention is further illustrated by the following examples: The present invention relates to a process for the preparation of a medicament for the preparation of a medicament for the treatment of essential oils. , the compounds of the formula (I), (III), (III), (IV), (IV), (IV) and (IV). In combination with two compounds having an oxydi-tungic effect, the maleic or the insane, potentiates the final effect, in fact, the invention has been reached. Accordingly, the general disclosure of the invention is directed to a preferred embodiment of the present invention. a new pharmaceutical formula for vaginal application, resulting from the association of two products in the taxai a, qui tostro and the essential oil of ibiza capi cata.

This product differs from other semi-vaginal products already. developed by their ovaries, drug materials of the natural carotene, top-of-the-art technologies and the characteristics of the outer tissues expressed by the association of two active saturated products; the chitosan and the essential oil of Td capitan stone.

Description <! &Amp; The invention relates to a process for the preparation of a pharmaceutical composition according to the invention. smooth, and pleasant odor, and is believed to be of the natural extract of TryoOra capita, a. of figure 1, the prepared bibrogei is illustrated.

This gel being in, t wheels gone into the vagina will be spread around the area and thanks to the characteristic.iscitas stickers of qtdtosa.no will have. ability to adhere to the walls of the vaginal epithelium as well as the effect of runoff. and increasing the time of permanence in the vagina, prodoriodo therapeutic effect. This effect can be enhanced by the addition of the polymers: cellulose: hydroxyalkylcellulose, hydroxypropylcellulose, carboxymethylcellulose, hydroxypropylcellulose, and the like; (5, 5-101), poloxamers and 0.5-2% coatings, and to compound them in toluene reductions, to their application in the compositions of the invention. the semi-solid cakes (5 g) with a classic applicator, will condase the adjusted cocaine covered in the vaginal cavity, as well as the bergamot dispersion of the active substances. -propionic acid (Parma-Qui.mi.ca Su.r S. L, d); low molecular weight chitosan, triaoetin and lactic acid (Bigrim-Aldrich), Follsorbate BC essential oil of Caffeine and water, Figure 1 shows the utilizable raw materials and the concentrations included in the developed prototype. of the gel required am agitator in war Heidolpb RtR 2 i 01St

For the preparation of the gei opt imitou · " and a method that a a bar and. the following pus pus sounds: 1. Put the coraititainree to incorporate into the formation; The reaction mixture is cooled to 0 ° C. c ao ao; 3, add to my cures. Autoresor or Pol.isoruaco 30; In part, the oil is dried in the mixture of prop. According to another aspect of the invention there is provided a compound of formula (I): 5, inserting both of the alloys 00b comprised the smooth step of obtaining a homogeneous gel; 6. Keep under heat and cool. Heating is required and actual dissolution is required under a shaker.

It was necessary to define the concentrations to be used, and each, I erected, took '" if the starting point is taken as necessary. antimicrobial effect, based on pseudococcal studies. However, such technology allows for the inclusion of the carrier and the carrier; medium and high organic weights and extractions of plants such as chamomile, lavage, malvae, perennial herbaceous plants, and other plants such as lidocaine in concentrations ranging from 0.1 to 2%, based on the therapeutic objective predicted; is defined. Other polymers; derived from cellulose (N-hydroxyleteloseose, hydroxypropylcellulose, carboxymethyl cellulose, methylcellulose) in conformations of 0.5-101%, polyoxamines in 0.1-20% strength, and In a preferred embodiment of the present invention, the present invention provides a method for the preparation of a compound of formula (I) in the presence of a compound of formula Other combinations of these two product types, qitosan and plant extracts, may be proposed based on the technology herein protected, the organoleptic character of the gel was real sweated under the dot of or. the gel is carefully observed and analyzed for the following characteristics; (color, opacity and Oonogeneity), odor, softness to touch and odorability (which encompasses the ease of scattering the pH of the gel in the pH 744 The lactic acid, in addition to the dog, gives solubilisation of the quitestane, gives uxr: acidic pH to the gel (4> 55, corpseable with vaginal fluid (4.5-5.05), which is due to Cante This acid is physiological of the vagina and has therefore been shown to be the best option for inclusion and production of the gel,

The purpose of the present invention is to provide the treatment of vaginal infections caused by Carunda spp, and it has been found that the fungal anaerobic effect of each of the active products isolated from the willow (willow, b, F> et. 004; Paitiaeira-de-Oli; vav; et al., 2012; - Salooira-de-

Oliveira, A, et al., 2000, proceeded to the study of the anti-imperialist effect of the association, the principal method of cernionation (cheoberboarding) in the ferext concentrates of each uxr active compounds, guitosa and essential benzyl capita bead, and post-ferrally the prepared phyla product, the gel.

This technology has enabled the production of a new pharmaceutical fork resulting from the combination of these two natural and bio-active products in a homogeneous dispersion. the essential oil in the agar gel, A. noxrogeneidae of the final product was confirmed by microscopic observation,

It has been found on the basis of the classical methodology of Cackerboard that the combination of both natural products produced a potent antifungal action, and the reported inddsaidal effect for each of the products was summarized (Figure 2). The resultant gel of the present invention, evaluated on the basis of CLS M27-A.3, corroborated, is shown in that the addition of the product of the product required for the treatment of alopecia is less than that of each of the prodrugs In this way the prepared gel serves not only of the carrier to the essential oil but also because it is composed of chitosan, potency is the antifungal effect. The invention thus has a recombinant potent anuifunctional effect in the combination of chitosan to essential oil of Thrnappa epa tara, seven dictination of its final time and effects prodaaidos based on this technology, of the other products proposed as is the case of simple healthy quito gel without association of other active products, proposed by Prai.asa at ai. or of inert gels of polycarbobutyl in which essential oils are provided in which the antimicrobial effect is typically expressed by the extract of the powder as the base gel is simply the carrier (if desired) , A, B, A, B, C, A, B, C, A, B, C, A and B are the same as those described above. 37, (21, 1.51-111);

Seven potentiated antifungal effects observed in this study are presented as an advantage, the effect and / or effect of promoting a final product with lower conceptions of the active compounds than anticipating a lower toxicity of the product, since the use of lower concentrations of essential oil will correspond to the error probability of the system absorption; here. the presence of chitosan concentrations may lead to the risk of absorption of essential oil from the open epithelium of the thighs.

Description &

Figmza. The chemical composition of the qaitesane gel and essential oil of ibiza capr cata

Figure 2; Resulted from the cbsekerboard MIC; kidney concentration in the inhibitory; FXCl; integrated value of single and run-of-the-line supply agreements - and the value presented exceeds the potential

Figure. 3; The gel gel, shown in the following table, is presented by the application of Gaitosan and the plant extracts, prolyglycerol, tricetin, 1-acetic acid, 0- the prototype of the invention is shown in the accompanying drawings in the accompanying drawings of the various exemplary optical weights,

Only the characterization of biologically active products, such as the biological and physiological properties, at physiological pH enhances their ability to treat the disease as a result of the properties of or of the golthan's life.

CoviIhd and PBI, February 13, sdli

Claims (2)

1. Chytoin-oil gel and Tone-capillary oil for the treatment of vaginal vaginal oysters. The composition of the present invention is characterized in that it comprises 10 to 10% aqueous NaHCO 3 and 10 to 10% aqueous NaHCO 3 and 10% aqueous NaHCO 3. 1% n / a at 0% lactic acid content of 0% lactic acid enters from 0% to 100% polyacrylonitrile between 1% and 1% w / w to the essential of Thymbi-capitata from 0.1 to 1% o / m. 2, Gel. of anitensin and oil of Taytama capitata for the treatment of conditions according to the present invention. i) 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 from qnitonano and dl to ao vencia! of Thymbra canister for the treatment of candidiasis goes. vovagrsai, according to the king come. in the extracts of plants with camomile, linden, baldness, perpetual arachidonic acid and anaesthetics such as lithocaine and concentrations of between 0.1 and 2%. 0001.1.01, January 12, 2014