PL241064B1 - Method of producing for producing a viscoelastic gel supplementing the synovial fluid and a viscoelastic gel supplementing the synovial fluid - Google Patents

Abstract

The subject of the application is a method of producing a viscoelastic gel replenishing synovial fluid and a viscoelastic gel replenishing synovial fluid, widely used in veterinary medicine to alleviate symptoms of joint dysfunction by increasing slip on the surface of connective tissue. The method consists in mixing an aqueous solution of a polymer with an average molecular weight above 1500 Da and having a concentration of 0.1% to 40% by weight, preferably polyvinylpyrrolidone and / or polyvinyl alcohol and / or hyaluronic acid, is mixed with an aqueous solution of a gold colloid with a concentration of 0.001% to 0.04% by weight and / or an aqueous solution of silver colloid at a concentration of 0.001% to 0.04% by weight, with one part by volume of the aqueous polymer solution mixed with at least 15 minutes with one to four parts by volume of the aqueous solution gold and / or silver colloid. The viscoelastic gel contains water in which a polymer with an average molecular weight above 1500 Da is dissolved in an amount of 0.08% by weight to 26.6% by weight, preferably polyvinylpyrrolidone and / or polyvinyl alcohol and / or hyaluronic acid, and colloidal gold in an amount from 0.0025% by weight to 0.01% by weight and / or colloidal silver in an amount from 0.00025% by weight to 0.01% by weight.

Description

PL 241 064 B1

Description of the invention

The subject of the invention is a method of producing a viscoelastic gel replenishing synovial fluid and a viscoelastic gel replenishing synovial fluid, which is widely used in veterinary medicine to alleviate the symptoms of joint dysfunction by increasing slip on the surface of connective tissue.

An implantable hydrogel of an acrylamide copolymer for therapeutic purposes, known from Polish patent application No. P.327454, is a copolymer of N-substituted methacrylamide or acrylamide, a cross-linking agent and a material selected from the group consisting of complex sugar, sugar derivative, tissue adhesive peptide and polymer conjugate with anti-lipid derived antibodies, wherein the polymeric hydrogel is heterogeneous, flexibly deformable, and has an equilibrium water content of at least about 80%, and a method of preparation thereof. It can be used for tissue regeneration or organ repair, for example in the developing or adult nervous system.

The multifunctional biocompatible hydrogel and the production method are known from the European patent specification EP1438976. The hydrogel contains acrylamide in the amount of 1.3 - 15 by weight, N, N'-methylene-bis-acrylamide cross-linking agents in the amount of 0.004 - 0.975, N, N'-ethylene-bis-acrylamide in the amount of 0.004 - 5.1, poviargolum in the amount of 0.002 - 0.45 and water - up to 100. The hydrogel is produced by copolymerizing acrylamide with cross-linking agents in an aqueous medium in the presence of a polymerization initiator, with the maturation of the reaction compound in two stages, the first of which is carried out at a temperature of 20 - 90 ° C for 2 - 24 hours, and the second - at 107 - 130 ° C for not more than 2 hours.

Leaflet on liquid synovial endoprosthesis available on the website: http://www.noltrex.pl/wp-content/uploads/2018/04/Folder-Noltrex.pdf, contains purified water in the amount of 1.5% - 96%, three-dimensional polyacrylamide in an amount of 1.5% - 4% and silver ions in an amount of 0.0001 0.0025%.

The essence of the method according to the invention consists in the fact that an aqueous polymer solution with an average molecular weight above 1500 Da and with a concentration of 0.1% to 40% by weight, selected from the group of polyvinylpyrrolidone, polyvinyl alcohol, hyaluronic acid and their mixtures, is mixed with an aqueous gold colloid solution with a concentration of 0.001% to 0.04% by weight and / or an aqueous silver colloid solution with a concentration from 0.001% to 0.04% by weight, where one part by volume of the aqueous polymer solution is mixed with at least 15 minutes with one to four parts by volume of an aqueous solution of gold and / or silver colloid.

The essence of the viscoelastic gel according to the invention consists in the fact that it contains water in which a polymer with an average molecular weight above 1500 Da is dissolved in an amount of 0.08% by weight to 26.6% by weight, selected from the group of polyvinylpyrrolidone, polyvinyl alcohol , hyaluronic acid and mixtures thereof, and colloidal gold in an amount from 0.0025 wt% to 0.01 wt% and / or colloidal silver in an amount from 0.00025 wt% to 0.01 wt%.

The viscoelastic gel replenishing synovial fluid according to the invention restores the proper level of viscosity of the synovial fluid, alleviates the symptoms of joint dysfunction, increases the glide on the surface of the connective tissue, limits further damage to the articular cartilage, alleviates pain by reducing friction between joint structures in the joint, absorbs the forces affecting the structures joint in the pond, improves the function of the joint. In addition, it contains silver and / or gold nanoparticles that are used as bactericidal material, as a drug carrier, as well as for the formation of gels and ointments with bactericidal properties.

The objects of the inventions are explained in the working examples.

P r z k ł a d 1

The method of obtaining viscoelastic gel replenishing synovial fluid consists in preparing 500 ml of an aqueous solution containing 24 wt. % polyvinylpyrrolidone and 0.1 wt. of hyaluronic acid, which is then mixed with 250 ml of 0.04 wt.% aqueous colloidal gold solution. and 250 ml of an aqueous solution of colloidal silver at a concentration of 0.001 wt. Everything is mixed for a minimum of 30 minutes. The viscoelastic gel prepared in this way replenishing the synovial fluid, dissolved in water, contains: 12 wt. % polyvinylpyrrolidone, 0.05 wt. % hyaluronic acid, 0.01 wt. colloidal gold and 0.00025 wt. colloidal silver.

PL 241 064 B1

P r z k ł a d 2

The method of synthesizing a viscoelastic gel replenishing synovial fluid consists in making up to 500 ml of an aqueous solution containing 12 wt. % polyvinyl alcohol and 10 wt. of polyvinylpyrrolidone, 250 ml of 0.01 wt.% aqueous colloidal gold solution are added. and 250 ml of an aqueous solution of colloidal silver at a concentration of 0.04% by weight. The mixture is stirred for 2 hours. The viscoelastic gel prepared in this way replenishing the synovial fluid contains: 6 wt. % polyvinyl alcohol, 5 wt. % polyvinylpyrrolidone, 0.0025 wt. colloidal gold and 0.01 wt. colloidal silver.

P r z k ł a d 3

The method of synthesizing the viscoelastic gel replenishing synovial fluid consists in preparing 500 ml of an aqueous solution containing 12 wt. % polyvinyl alcohol and 0.6 wt. of hyaluronic acid, and 250 ml of an aqueous colloidal gold solution with a concentration of 0.02% by weight is added thereto. and 250 ml of an aqueous solution of colloidal silver at a concentration of 0.02% by weight. The mixture is stirred for 1 hour. The viscoelastic gel prepared in this way replenishing the synovial fluid contains: 6 wt. polyvinyl alcohol, 0.3 wt. % hyaluronic acid, 0.005 wt. colloidal gold and 0.005 wt. colloidal silver.

P r z k ł a d 4

The method of synthesizing the viscoelastic gel replenishing synovial fluid consists in preparing 500 ml of an aqueous solution containing 12 wt. % polyvinyl alcohol, 12 wt. % polyvinylpyrrolidone and 0.2 wt. of hyaluronic acid and 250 ml of an aqueous colloidal gold solution with a concentration of 0.001% by weight is added thereto. and 250 ml of an aqueous solution of colloidal silver at a concentration of 0.01% by weight. The whole is stirred for 30 minutes. The viscoelastic gel prepared in this way replenishing the synovial fluid contains: 6 wt. % polyvinyl alcohol, 6 wt. polyvinylpyrrolidone, 0.1 wt. % hyaluronic acid, 0.00025 wt. colloidal gold and 0.0025 wt. colloidal silver.

P r z k ł a d 5

The method of synthesizing the viscoelastic gel replenishing synovial fluid consists in preparing 500 ml of an aqueous solution containing 24 wt. polyvinyl alcohol, and 500 ml of 0.02 wt.% aqueous gold colloidal solution is added thereto. The whole is mixed for 15 minutes. The viscoelastic gel prepared in this way replenishing the synovial fluid, dissolved in water, contains: 12 wt. % polyvinyl alcohol and 0.01 wt. colloidal gold.

P r x l a d 6

The method of synthesizing the viscoelastic gel replenishing synovial fluid consists in preparing 500 ml of an aqueous solution containing 24 wt. of polyvinylpyrrolidone and 500 ml of an aqueous solution of colloidal silver with a concentration of 0.001% by weight is added thereto. The whole is stirred for 15 minutes. The viscoelastic gel prepared in this way replenishing the synovial fluid, dissolved in water, contains: 12 wt. % polyvinylpyrrolidone and 0.0005 wt. colloidal silver.

P r z k ł a d 7

The method of synthesizing the viscoelastic gel replenishing synovial fluid consists in preparing 500 ml of an aqueous solution containing 2 wt. of hyaluronic acid and 500 ml of an aqueous colloidal gold solution with a concentration of 0.04% by weight is added thereto. The whole is stirred for 30 minutes. The viscoelastic gel prepared in this way replenishing the synovial fluid, dissolved in water, contains: 1 wt. % of hyaluronic acid and 0.02 wt. colloidal gold.

P r z k ł a d 8

The method of synthesizing the viscoelastic gel replenishing synovial fluid consists in preparing 500 ml of an aqueous solution containing 40 wt. polyvinyl alcohol, and 200 ml of an aqueous colloidal gold solution with a concentration of 0.04% by weight is added thereto. and 50 ml of an aqueous solution of colloidal silver at a concentration of 0.024% by weight. The mixture is stirred for 1 hour. The viscoelastic gel prepared in this way replenishing the synovial fluid contains dissolved in water: 26.6 wt. % polyvinyl alcohol, 0.0064 wt. colloidal gold and 0.0016 wt. colloidal silver.

P r z k ł a d 9

The method of synthesizing the viscoelastic gel replenishing synovial fluid consists in preparing 250 ml of an aqueous solution containing 24 wt. polyvinyl alcohol, and 500 ml of 0.012 wt.% aqueous gold colloidal solution is added thereto. The mixture is stirred for 1 hour. The viscoelastic gel prepared in this way replenishing the synovial fluid contains dissolved in water: 8 wt. % polyvinyl alcohol and 0.008 wt. colloidal gold.

Claims (2)

PL 241 064 B1 P r z k ł a d 10 The method of synthesizing the viscoelastic gel replenishing synovial fluid consists in preparing 250 ml of an aqueous solution containing 4 wt. of hyaluronic acid, and 750 ml of an aqueous colloidal gold solution with a concentration of 0.03% by weight is added thereto. and 250 ml of an aqueous solution of colloidal silver at a concentration of 0.004 wt. The mixture is stirred for 2 hours. The viscoelastic gel prepared in this way replenishing the synovial fluid contains dissolved in water: 0.8 wt. % hyaluronic acid, 0.018 wt. colloidal gold and 0.0008 wt. colloidal silver. Patent claims A method of producing a viscoelastic gel replenishing synovial fluid, characterized in that an aqueous polymer solution with an average molecular weight above 1500 Da and a concentration ranging from 0.1% to 40% by weight, selected from the group of polyvinylpyrrolidone, polyvinyl alcohol, hyaluronic acid and their of the mixtures, mixed with an aqueous gold colloid solution with a concentration of 0.001% to 0.04% by weight and / or an aqueous silver colloid solution with a concentration of 0.001% to 0.04% by weight, where one part by volume of the aqueous polymer solution is mixed with at least 15 minutes with one to four parts by volume of an aqueous solution of gold and / or silver colloid. 2. Visco-elastic gel replenishing synovial fluid containing an aqueous polymer solution, characterized in that it contains water in which the polymer with an average molecular weight above 1500 Da is dissolved in an amount of 0.08% by weight to 26.6% by weight, selected from the group polyvinylpyrrolidone, polyvinyl alcohol, hyaluronic acid and mixtures thereof, and colloidal gold in an amount from 0.0025 wt% to 0.01 wt% and / or colloidal silver in an amount from 0.00025 wt% to 0.01 wt%.