PE20001068A1 - Produccion de analogos de tripsinogeno recombinante solubles - Google Patents
Produccion de analogos de tripsinogeno recombinante solublesInfo
- Publication number
- PE20001068A1 PE20001068A1 PE1999000952A PE00095299A PE20001068A1 PE 20001068 A1 PE20001068 A1 PE 20001068A1 PE 1999000952 A PE1999000952 A PE 1999000952A PE 00095299 A PE00095299 A PE 00095299A PE 20001068 A1 PE20001068 A1 PE 20001068A1
- Authority
- PE
- Peru
- Prior art keywords
- amino acids
- asp
- arg
- trypsinogen
- seq
- Prior art date
Links
- 102000018690 Trypsinogen Human genes 0.000 title abstract 5
- 108010027252 Trypsinogen Proteins 0.000 title abstract 5
- 150000001413 amino acids Chemical class 0.000 abstract 12
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 abstract 2
- 102000004400 Aminopeptidases Human genes 0.000 abstract 2
- 108090000915 Aminopeptidases Proteins 0.000 abstract 2
- 102000004142 Trypsin Human genes 0.000 abstract 2
- 108090000631 Trypsin Proteins 0.000 abstract 2
- 239000012588 trypsin Substances 0.000 abstract 2
- GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical class NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 abstract 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000013467 fragmentation Methods 0.000 abstract 1
- 238000006062 fragmentation reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
SE REFIERE A UN ANALOGO DE TRIPSINOGENO RECOMBINANTE (QUE PUEDE ACTIVARSE POR FRAGMENTACION CON UNA AMINOPEPTIDASA) QUE COMPRENDE: a)UNA PROTEINA QUE TIENE ACTIVIDAD DE TRIPSINA; b)UNA SECUENCIA QUE TIENE AL MENOS 2 AMINOACIDOS EXCEPTO Lys o Arg; Y SE SELECCIONADA DE i)DE 2 A 20 AMINOACIDOS NO BASICOS; ii)DE 2 a 20 RESIDUOS DE AMINOACIDOS EXCEPTO Lys O Arg; iii)Val-Asp-Asp-Asp-Asp-N, DONDE N ES CUALQUIER AMINOACIDO EXCEPTO Lys, Arg Y Pro; iv)AMINOACIDOS 5-10 DE LA SEC ID NO:2 (SECUENCIA DE 233 AMINOACIDOS); v)DE 2 A 20 AMINOACIDOS O AMINOACIDOS HIDROFILICOS QUE NO SEAN Lys o Arg; vi)RESIDUOS DE AMINOACIDOS 5-8 DE LA SEC ID NO:2; vii)DE 2 A 20 AMINOACIDOS DONDE EL NUMERO DE AMINOACIDOS ES PAR Y LOS RESIDUOS SON CUALQUIER AMINOACIDO NO BASICO EXCEPTO PROLINA. TAMBIEN SE REFIERE A ACIDOS NUCLEICOS AISLADO QUE CODIFICA EL ANALOGO DE TRIPSINOGENO Y TRIPSINA CONSISTE DE LAS BASES 4-699 DE LA SEC NO:3 (DE 699 BASES); UN VECTOR DE EXPRESION QUE COMPRENDE UN COMPUESTO NUCLEOTIDO AISLADO UNIDO A UNA SECUENCIA DE CONTROL DE EXPRESION Y UN METODO PARA PRODUCIR TRIPSINA RECOMBINANTE (SEC ID NO 4 DE 231 AMINOACIDOS) QUE COMPRENDE EXPRESAR EL TRIPSINOGENO EN UNA CELULA HUESPED; AISLAR EL TRIPSINOGENO EXPRESADO; ACTIVAR EL TRIPSINOGENO AISLADO CON UNA AMINOPEPTIDASA
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10121398P | 1998-09-21 | 1998-09-21 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PE20001068A1 true PE20001068A1 (es) | 2000-10-18 |
Family
ID=22283537
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PE1999000952A PE20001068A1 (es) | 1998-09-21 | 1999-09-20 | Produccion de analogos de tripsinogeno recombinante solubles |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1141263A4 (es) |
| AR (1) | AR021818A1 (es) |
| AU (1) | AU6388499A (es) |
| CA (1) | CA2343966A1 (es) |
| PE (1) | PE20001068A1 (es) |
| WO (1) | WO2000017332A1 (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU7053500A (en) * | 1999-09-15 | 2001-04-17 | Eli Lilly And Company | Chymotrypsin-free trypsin |
| DK1399568T3 (da) | 2001-02-01 | 2006-03-27 | Hoffmann La Roche | Fremgangsmåde til fremstilling af rekombinant trypsin |
| ATE439432T1 (de) | 2002-08-30 | 2009-08-15 | Novozymes Inc | Verfahren zur herstellung von säugetier-trypsinen |
| CN104312933B (zh) * | 2014-10-17 | 2017-03-29 | 江南大学 | 一种优化信号肽提高胰蛋白酶胞外分泌表达的方法 |
| CA3006175A1 (en) | 2015-11-25 | 2017-06-01 | Academisch Ziekenhuis Leiden | Recombinant serine proteases |
| CN116445462A (zh) * | 2023-04-20 | 2023-07-18 | 西安麦博泰克生物科技有限公司 | 一种重组猪胃蛋白酶的纯化制备方法及重组猪胃蛋白酶 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5763215A (en) * | 1984-08-16 | 1998-06-09 | Bio-Technology General Corporation | Method of removing N-terminal amino acid residues from eucaryotic polypeptide analogs and polypeptides produced thereby |
| TW257792B (es) * | 1992-10-01 | 1995-09-21 | Lilly Co Eli | |
| CA2102673A1 (en) * | 1992-11-13 | 1994-05-14 | Michael G. Greaney | Expression vectors for bovine trypsin and trypsinogen and host cells transformed therewith |
| US5773248A (en) * | 1995-11-13 | 1998-06-30 | Uab Research Foundation | Nucleic acid encoding a human α3(IX) collagen protein and method of producing the protein recombinantly |
| JPH09294583A (ja) * | 1996-03-08 | 1997-11-18 | Ajinomoto Co Inc | アミノペプチダーゼｇｘ及びそれを用いるタンパク質の加水分解方法 |
-
1999
- 1999-09-15 WO PCT/US1999/021047 patent/WO2000017332A1/en not_active Application Discontinuation
- 1999-09-15 CA CA002343966A patent/CA2343966A1/en not_active Abandoned
- 1999-09-15 AU AU63884/99A patent/AU6388499A/en not_active Abandoned
- 1999-09-15 EP EP99951445A patent/EP1141263A4/en not_active Withdrawn
- 1999-09-20 PE PE1999000952A patent/PE20001068A1/es not_active Application Discontinuation
- 1999-09-20 AR ARP990104729A patent/AR021818A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2343966A1 (en) | 2000-03-30 |
| AU6388499A (en) | 2000-04-10 |
| WO2000017332A1 (en) | 2000-03-30 |
| AR021818A1 (es) | 2002-08-07 |
| EP1141263A4 (en) | 2002-08-28 |
| EP1141263A1 (en) | 2001-10-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FC | Refusal |