NZ333996A - Method and vaccine containing enteric bacteria with antigenic properties - Google Patents
Method and vaccine containing enteric bacteria with antigenic propertiesInfo
- Publication number
- NZ333996A NZ333996A NZ333996A NZ33399695A NZ333996A NZ 333996 A NZ333996 A NZ 333996A NZ 333996 A NZ333996 A NZ 333996A NZ 33399695 A NZ33399695 A NZ 33399695A NZ 333996 A NZ333996 A NZ 333996A
- Authority
- NZ
- New Zealand
- Prior art keywords
- culture
- phase
- egta
- bacteria
- air
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title abstract 4
- 230000000890 antigenic effect Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 229960005486 vaccine Drugs 0.000 title 1
- 230000005526 G1 to G0 transition Effects 0.000 abstract 2
- 241000589989 Helicobacter Species 0.000 abstract 2
- YJIYWYAMZFVECX-UHFFFAOYSA-N 2-[N-[2-(acetyloxymethoxy)-2-oxoethyl]-2-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]anilino]acetic acid acetyloxymethyl ester Chemical compound CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=CC=CC=C1OCCOC1=CC=CC=C1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O YJIYWYAMZFVECX-UHFFFAOYSA-N 0.000 abstract 1
- 241000607534 Aeromonas Species 0.000 abstract 1
- 241000606125 Bacteroides Species 0.000 abstract 1
- 241000589876 Campylobacter Species 0.000 abstract 1
- 241000193403 Clostridium Species 0.000 abstract 1
- 241000588914 Enterobacter Species 0.000 abstract 1
- 241000194033 Enterococcus Species 0.000 abstract 1
- 241000607142 Salmonella Species 0.000 abstract 1
- 241000607768 Shigella Species 0.000 abstract 1
- 241000607598 Vibrio Species 0.000 abstract 1
- 241000607734 Yersinia <bacteria> Species 0.000 abstract 1
- GNJXVFXIDHCCKR-UHFFFAOYSA-N acetyloxymethyl 2-[[2-(acetyloxymethoxy)-2-oxoethyl]-[2-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]ethoxy]ethoxy]ethyl]amino]acetate Chemical compound CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)CCOCCOCCN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O GNJXVFXIDHCCKR-UHFFFAOYSA-N 0.000 abstract 1
- 230000003698 anagen phase Effects 0.000 abstract 1
- 210000004556 brain Anatomy 0.000 abstract 1
- 244000309466 calf Species 0.000 abstract 1
- 150000001768 cations Chemical class 0.000 abstract 1
- 239000002738 chelating agent Substances 0.000 abstract 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 abstract 1
- 230000002163 immunogen Effects 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000001802 infusion Methods 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
A method of producing an enteric bacterium having enhanced antigenic properties selected from: Campylobacter sp, Yersinia sp, Bacteroides sp, Klebiella sp, Enterobacter sp, Gastrospirillum sp, Salmonella sp, Shigella, sp Aeromonas, Vibrio sp Clostridium sp, Enterococcus sp and Eschericha coli Comprising growing a culture of enteric bacteria in vitro in a combination of conditions comprising; a) a divalent cation chelator selected from 1.0-25ìM of BAPTA/AM, 0.5-10ìM EGTA and 1.0-100ìM EGTA/AM; b) at a temperature between 30-42oC; c) in air or an microaerophillic condition, wherein the microaerophillic condition comprises i) 5-20% CO2 with 80-95% air ii) 5-10% O2 with 10-20% CO2 with about 70-85% N2 for a sufficient time so that the culture is in a growth phase at about early log phase, between early log phase and stationary phase, or at about stationary phase, wherein the enhanced antigenic property is a higher level of immunogenic antigenicity when compared to the antigenic property of bacteria from a culture of Helicobacter species in brain heart infusion broth supplemented with bovine calf serum.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US31840994A | 1994-10-05 | 1994-10-05 | |
| US08/538,544 US5897475A (en) | 1994-10-05 | 1995-10-03 | Vaccines comprising enhanced antigenic helicobacter spp. |
| US08/538,543 US5681736A (en) | 1994-10-05 | 1995-10-03 | Methods for producing enhanced antigenic shigella bacteria and vaccines comprising same |
| NZ295877A NZ295877A (en) | 1994-10-05 | 1995-10-04 | A vaccine comprising the helicobacter bacterium and its use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ333996A true NZ333996A (en) | 2000-07-28 |
Family
ID=27484355
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ333996A NZ333996A (en) | 1994-10-05 | 1995-10-04 | Method and vaccine containing enteric bacteria with antigenic properties |
Country Status (1)
| Country | Link |
|---|---|
| NZ (1) | NZ333996A (en) |
-
1995
- 1995-10-04 NZ NZ333996A patent/NZ333996A/en unknown
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| RENW | Renewal (renewal fees accepted) | ||
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