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NO20080062L - Fremgangsmate for pavisning av mikroorganisme og kit for pavisning av mikroorganisme - Google Patents

Fremgangsmate for pavisning av mikroorganisme og kit for pavisning av mikroorganisme

Info

Publication number
NO20080062L
NO20080062L NO20080062A NO20080062A NO20080062L NO 20080062 L NO20080062 L NO 20080062L NO 20080062 A NO20080062 A NO 20080062A NO 20080062 A NO20080062 A NO 20080062A NO 20080062 L NO20080062 L NO 20080062L
Authority
NO
Norway
Prior art keywords
microorganism
paving
test sample
kit
dna
Prior art date
Application number
NO20080062A
Other languages
English (en)
Inventor
Shinichi Yoshida
Takashi Soejima
Original Assignee
Morinaga Milk Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Morinaga Milk Industry Co Ltd filed Critical Morinaga Milk Industry Co Ltd
Publication of NO20080062L publication Critical patent/NO20080062L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/519—Topoisomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Levende celler som stammer fra en mikroorganisme i en test-prøve blir detektert ved hjelp av følgende trinn: a) trinnet som innebærer behandling av test-prøven med en topoisomerase-gift og/eller en DNA gyrase-gift, b) trinnet som omfatter ekstraksjon av DNA fra test-prøven og amplifikasjon av en målregion fra det ekstraherte DNA ved hjelp av en PCR-reaksjon, og c) trinnet som innebærer å analysere det amplifiserte produktet.
NO20080062A 2006-02-17 2008-01-04 Fremgangsmate for pavisning av mikroorganisme og kit for pavisning av mikroorganisme NO20080062L (no)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2006/302893 WO2007094077A1 (ja) 2006-02-17 2006-02-17 微生物検出法及び微生物検出キット

Publications (1)

Publication Number Publication Date
NO20080062L true NO20080062L (no) 2008-11-17

Family

ID=38371268

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20080062A NO20080062L (no) 2006-02-17 2008-01-04 Fremgangsmate for pavisning av mikroorganisme og kit for pavisning av mikroorganisme

Country Status (9)

Country Link
US (2) US9139866B2 (no)
EP (1) EP1992690B1 (no)
JP (1) JP4127847B2 (no)
KR (2) KR20100115383A (no)
CN (1) CN101341249B (no)
AU (1) AU2006338402B2 (no)
CA (1) CA2615089C (no)
NO (1) NO20080062L (no)
WO (1) WO2007094077A1 (no)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2548222T3 (es) 2006-02-28 2015-10-14 Montana State University Uso de derivados de fenantridio para diferenciar entre células intactas y con membrana comprometida usando técnicas moleculares a base de ácido nucleico
KR100955884B1 (ko) * 2008-04-07 2010-05-06 고려대학교 산학협력단 살리신을 이용한 엔테로박터 사카자키의 검출방법 및 이를이용한 엔테로박터 사카자키의 선택배지
JP2010017144A (ja) * 2008-07-11 2010-01-28 National Institute Of Infectious Diseases レジオネラ属菌の検出方法及び検出キット
WO2011010740A1 (ja) 2009-07-24 2011-01-27 森永乳業株式会社 微生物検出法及び微生物検出キット
JP5692489B2 (ja) * 2010-01-15 2015-04-01 東洋製罐グループホールディングス株式会社 微生物の検出方法
JP5766216B2 (ja) 2011-01-24 2015-08-19 タカラバイオ株式会社 核酸修飾方法
PT2751083T (pt) * 2011-08-31 2018-03-05 Otsuka Pharma Co Ltd Composto de quinolona
DE102012014981B3 (de) 2012-07-26 2013-07-18 Sartorius Stedim Biotech Gmbh Verfahren zur Differenzierung zwischen lebenden und toten Zellen
WO2014021351A1 (ja) * 2012-08-03 2014-02-06 森永乳業株式会社 微生物検出法及び微生物検出キット
JP5433820B1 (ja) * 2012-08-03 2014-03-05 森永乳業株式会社 微生物検出法及び微生物検出キット
FI128362B (en) 2015-02-27 2020-04-15 Kemira Oyj Procedure for Quantitative Monitoring of Endospores in the Aquatic Environment of a Paper or Cardboard Factory
CN107923825A (zh) * 2015-07-23 2018-04-17 普度研究基金会 快速浓缩、回收和检测食物样品中的病原体
KR101760726B1 (ko) 2015-12-18 2017-07-24 대한민국 (식품의약품안전처장) 미생물 탈리 및 페놀-클로로포름을 이용한 동물성 식품으로부터 메타게놈 dna의 분리방법
DE102020103971B4 (de) * 2020-02-14 2022-02-24 Testo bioAnalytics GmbH Verfahren zum Nachweis von lebenden Mikroorganismen und ein fluidisches Kanalsystem
CN113528684A (zh) * 2021-08-04 2021-10-22 领航基因科技（杭州）有限公司 用于鉴别革兰氏阴性菌和革兰氏阳性菌的数字pcr检测试剂盒和应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU615286B2 (en) 1988-04-15 1991-09-26 N.V. Innogenetics S.A. Hybridization probes for detecting neisseria strains
CA2258547A1 (en) 1996-06-17 1997-12-24 Microcide Pharmaceuticals, Inc. Screening methods using microbial strain pools
JP2001149073A (ja) * 1999-09-16 2001-06-05 Sanyo Electric Co Ltd 腸内細菌叢の分析方法及び分析装置
GB0008787D0 (en) 2000-04-10 2000-05-31 Norsk Naeringsmiddelforskning A method of cell detection
WO2002052034A1 (fr) 2000-12-26 2002-07-04 Joji Oshima Methodes de bioscopie et d'amplification d'acides nucleiques
US20030130171A1 (en) * 2001-10-30 2003-07-10 Schoenhard Grant L. Inhibitors of ABC drug transporters in multidrug resistant microbial cells
WO2004015141A2 (en) 2002-08-08 2004-02-19 University Of Lausanne Method for the rapid assessment of the presence and viability of bacterial cells and use thereof________________________
FI20031678A0 (fi) * 2003-11-18 2003-11-18 Teemu Korpimaeki Näytteen käsittelymenetelmä ja järjestely siihen
CA2666448C (en) * 2007-08-16 2013-09-10 Morinaga Milk Industry Co., Ltd. Method and kit for the detection of live microorganism cells in a sample

Also Published As

Publication number Publication date
JP4127847B2 (ja) 2008-07-30
CN101341249B (zh) 2013-07-24
KR20100115383A (ko) 2010-10-27
WO2007094077A1 (ja) 2007-08-23
US20150086995A1 (en) 2015-03-26
JPWO2007094077A1 (ja) 2009-07-02
KR101010122B1 (ko) 2011-01-24
CA2615089C (en) 2015-11-17
EP1992690A1 (en) 2008-11-19
US20090305240A1 (en) 2009-12-10
US9567625B2 (en) 2017-02-14
AU2006338402A1 (en) 2007-08-23
US9139866B2 (en) 2015-09-22
KR20080027848A (ko) 2008-03-28
EP1992690B1 (en) 2014-05-21
CA2615089A1 (en) 2007-08-23
EP1992690A4 (en) 2009-09-23
CN101341249A (zh) 2009-01-07
AU2006338402B2 (en) 2010-12-23

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