NL2031127B1 - Method for promoting germination of mallotus apelta (lour.) müll. arg. seeds - Google Patents
Method for promoting germination of mallotus apelta (lour.) müll. arg. seeds Download PDFInfo
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- NL2031127B1 NL2031127B1 NL2031127A NL2031127A NL2031127B1 NL 2031127 B1 NL2031127 B1 NL 2031127B1 NL 2031127 A NL2031127 A NL 2031127A NL 2031127 A NL2031127 A NL 2031127A NL 2031127 B1 NL2031127 B1 NL 2031127B1
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- seeds
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- apelta
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- 230000035784 germination Effects 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 17
- 230000001737 promoting effect Effects 0.000 title claims abstract description 6
- 241001139545 Mallotus apelta Species 0.000 title claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000005286 illumination Methods 0.000 claims abstract description 11
- 239000012153 distilled water Substances 0.000 claims abstract description 8
- 229920001817 Agar Polymers 0.000 claims abstract description 6
- 239000008272 agar Substances 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims abstract description 4
- 238000009331 sowing Methods 0.000 claims abstract description 3
- 230000002745 absorbent Effects 0.000 claims abstract 2
- 239000002250 absorbent Substances 0.000 claims abstract 2
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims description 4
- 238000005201 scrubbing Methods 0.000 abstract description 11
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 abstract description 5
- 229930191978 Gibberellin Natural products 0.000 abstract description 2
- 239000003448 gibberellin Substances 0.000 abstract description 2
- 238000005406 washing Methods 0.000 abstract description 2
- 238000011282 treatment Methods 0.000 description 14
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 239000005980 Gibberellic acid Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 208000032843 Hemorrhage Diseases 0.000 description 2
- 208000034158 bleeding Diseases 0.000 description 2
- 231100000319 bleeding Toxicity 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- 206010008909 Chronic Hepatitis Diseases 0.000 description 1
- 240000000491 Corchorus aestuans Species 0.000 description 1
- 235000011777 Corchorus aestuans Nutrition 0.000 description 1
- 235000010862 Corchorus capsularis Nutrition 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000221017 Euphorbiaceae Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 206010033078 Otitis media Diseases 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 206010053476 Traumatic haemorrhage Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 208000002399 aphthous stomatitis Diseases 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 229910052729 chemical element Inorganic materials 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 230000035611 feeding Effects 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 206010019847 hepatosplenomegaly Diseases 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- -1 peroxide peroxide Chemical class 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 238000004162 soil erosion Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000008736 traumatic injury Effects 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present disclosure discloses a method for promoting germination of Nbllotus apelta(Lour.) Mull. Arg. seeds. The method comprises the following steps: putting the Nbllotus apelta(Lour.) Mull .Arg. seeds into distilled water, and standing at 20°C for 4 hours, then taking out the soaked seeds, scrubbing to remove black loose oil—containing structures on the surfaces, washing with clear water, and sucking up water on the surfaces of the seeds with absorbent paper; sowing the treated seeds on a 1% agar culture medium containing 200 mg/L of gibberellin, and germinating the culture medium at 20°C, wherein the illumination intensity is 1000 lux, and the photoperiod is 12 hours of illumination/12 hours of darkness. By using the method of the present disclosure, the germination rate of Nbllotus apelta(Lour.) Mull. Arg. seeds can be increased from 30% to nearly 66.7%.
Description
P1206/NLpd
METHOD FOR PROMOTING GERMINATION OF MALLOTUS APELTA (LOUR.) MULL.
ARG. SEEDS
The present disclosure belongs to the technical field of ger- mination of plant seeds, and particularly relates to a method for promoting germination of Mallotus apelta (Lour.) Müll.Arg. seeds.
Mallotus apelta (Lour.) Mall. Arg. is shrub or small tree species of Euphorbiaceae. It often grows on the roadsides of flat hills and barren and dry bare mountains or barren mountains in southern provinces of China, it is a pioneer species and can be used for preventing soil erosion and ecological restoration. Its tender leaves in spring can be mixed with other fodders for feed- ing pigs, and the leaves can be collected for preparing compost in winter. Its stem barks are fibrous raw materials and can be woven into jute bags or used as blended yarns or used as raw materials of wax paper and artificial cotton. The roots and leaves of M. apelta are used as medicines; the leaves have the effects of clearing heat, diuresis, relieving pain, removing toxicity and stopping bleeding and can be used for treating otitis media, aph- tha, traumatic injuries, eczema, traumatic bleeding, etc.; and the roots have the effects of nourishing the liver, activating blood circulation, and can be used for treating chronic hepatitis, hepatosplenomegaly, edema and other diseases; and meanwhile, the roots can also be used for treating stomachache, vomiting, bleed- ing, skin wet itching and other diseases. The oil content of the seeds reaches 36%, and can be used for preparing bactericides, lubricants, etc.. Although M. apelta is widely distributed in southern China, the resource quantity is relatively low, and the propagation survival rate of M. apelta is not high and is only about 30% either using hard branch cuttage or twig cuttage. At present, the M. apelta cultivation technology is not efficient , there is no report of large-scale seedling propagation, and seed germination is still one of main propagation technologies.
At present, in the prior art, the germination rate of fresh
M. apelta seeds is about 30-50%.
The present disclosure aims to provide a simple and efficient method for germinating M. apelta seeds so as to improve the germi- nation rate of the M. apelta seeds. The method can improve the germination rate of the M. apelta seeds from 30% to 66.7%, and is simple and easy to implement, and has no risk or toxic or side ef- fect.
In order to achieve the above object, the present disclosure provides the following technical solutions:
A method for promoting germination of M. apelta seeds com- prises the following steps: (1) immerging the seeds into distilled water at 20°C for 4 hours, then taking out the soaked seeds, scrubbing to remove black loose oil-containing structures on the surfaces, washing with clear running water, any excess moisture was removed by blotting the seeds surface dry; and (2) sowing the seeds treated in the step (1) on a 1% agar medium containing 200 mg/L of gibberellin, and incubated at 20°C, wherein the illumination intensity is 1000 lux, and the photoperi- od is 12 hours of illumination/12 hours of darkness.
The substantive content of the present disclosure is further described below with the embodiments herein, but the present dis- closure is not limited by them.
Example 1: 1. Materials and method: 1.1 Research materials:
In October 2015, ripe and fresh Mallotus apelta (Lour.) Mull.
Arg. seeds were collected from Guanyin Mountain, Yuanyang County,
Honghe Hani and Yi Autonomous Prefecture, Yunnan Province. After collection, they were transported to The Germplasm Bank of Wild
Species of Kunming Institute of Botany for preservation. Storage was performed under conditions of temperature of 15°C and relative humidity of 15%. The storage was performed for 10 months.
1.2 Research method:
1.2.1 Experimental design: seeds stored were directly taken out and divided into four treatment groups, namely a control group, a scrubbing group, a distilled water treated scrubbing group and a hydrogen peroxide treated scrubbing group.
The dis- tilled water treated scrubbing group and the hydrogen peroxide treated scrubbing group were respectively provided with three gra- dients, the three gradients included soaking for 4 hours, 8 hours and 16 hours.
Each treatment 3 replicates with 40 seeds for each replicate was used.
1.2.2 Treatment with distilled water: 360 seeds were put into three 35 ml glass vials with caps, and each vail contained 120 seeds; 20 ml of distilled water was respectively added, the caps were screwed, and the bottles were put into an illumination incu- bator at the temperature of 20°C. 120 seeds were randomly taken out in sequence after 4 hours, 8 hours and 16 hours and were lightly scrubbed on a gauze element to remove black loose oil-containing structures on the surface, then the seeds were washed with tap wa-
ter, any excess moisture was removed by blotting the seeds surface dry.
1.2.3 Treatment with hydrogen peroxide: 360 seeds were put into three 35 ml glass vials with caps, and each vail contained 120 seeds; 20 ml of 3-3.5% hydrogen peroxide/water solution was respectively added into each wail, the caps were screwed, and the bottles were put into an illumination incubator at 20°C; 120 seeds were randomly taken out in sequence after 4 hours, 8 hours and 16 hours and were slightly scrubbed on the gauze element to remove black loose oil-containing structures on the surfaces, then the seeds were washed with tap water; any excess moisture was removed by blotting the seeds surface dry.
1.2.4 Treatment by scrubbing: the seeds were soaked in water and then were immediately and slightly scrubbed on the gauze ele- ment to remove black loose oil-containing structures on the sur-
faces of the seeds, the seeds were washed with tap water, any ex- cess moisture was removed by blotting the seeds surface dry.
1.2.5 Germination: two culture media were adopted in the ex-
periment, wherein one medium was a common 1% agar medium, and the other medium was a 1% agar medium containing 200 mg/L of gibberel- lic acid {GA:). The seeds were respectively sowed on the two media, the Petri dishes were placed in transparent plastic bags to pre- vent desiccation., then the transparent plastic bags were put in an illumination incubator at 20°C, wherein the illumination inten- sity was 1000 lux, and the photoperiod was 12 hours of illumina- tion/12 hours of darkness. Germination was checked once every 2-3 days, the seeds can be determined to be germinated if radicles ex- tend to exceed 5 mm, the germinated seeds were taken out and the germination quantity was recorded; and the experiment was termi- nated if no germination occured for 2 consecutive weeks after the experiment’s onset for 4 weeks. 1.2.6 Data analysis: SPSS16.0 software was used for analyzing germination data, One-Way ANOVA was used for performing variance analysis, and an S-N-K method was used for performing multiple comparisons on germination rates of different treatments. 2. Results and discussion 2.1 Effect of different treatment methods on germination of
Mallotus apelta (Lour.) Mull. Arg. seeds:
Table 1 Effect of different treatment methods on germination of Mallotus apelta (Lour.) Mill. Arg. seeds
Table 1 Effect of different treatments on the germination rate of Mallotus apelta (Lour.) Mull. Arg. seeds (3) ~~ Control Only Treat Treat Treat Treat Treat Treatment scrubbing ment ment ment ment ment with hy- with with with with with drogen distilled distilled distilled hydrogen hydrogen peroxide water water water peroxide peroxide for 16 for 4 for 8 for 16 for4 for8 hours hours hours hours hours hours
Common 0 © OO medium
Gibberel- 30.0413.2 55.0+13.2 667417 3.343.3a 35.0+10 1674444 16.7433 31.7+60a lin- ab bc c Aab a b contain- ing medi- um
Notes: the result was represented by an average value = standard error, and different letters indicated that the germina- tion rates of different treatment on the - medium containing 200mg/L gibberellic acid (GA;) are significantly different. 5 As shown in Table 1, seeds could not germinate on a gibberel- lin-free common medium.
The germination rate of untreated seeds on an agar medium containing 200 mg/L gibberellic acid (GA:) was 30%, the germination rate of seeds subjected to scrubbing treatment af- ter soaking in distilled water for 4 hours could reach 66.7%, and the effect was the best in all treatment; the scrubbing effect had no significant difference from that after scaking in distilled wa- ter for 4 hours, but the black loose oil-containing structure on the outer layer of the scaked seeds was easier to remove. 3. The present disclosure has the following positive effects: the germination of Mallotus apelta (Lour.) Mull.
Arg. seeds was significantly promoted, the germination rate of M. apelta seeds was increased from 30% to 66.7%, and the treatment method was sim- ple and feasible, and had no risk or toxic or side effect.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2031127A NL2031127B1 (en) | 2022-03-01 | 2022-03-01 | Method for promoting germination of mallotus apelta (lour.) müll. arg. seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2031127A NL2031127B1 (en) | 2022-03-01 | 2022-03-01 | Method for promoting germination of mallotus apelta (lour.) müll. arg. seeds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NL2031127B1 true NL2031127B1 (en) | 2023-09-07 |
Family
ID=88064666
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NL2031127A NL2031127B1 (en) | 2022-03-01 | 2022-03-01 | Method for promoting germination of mallotus apelta (lour.) müll. arg. seeds |
Country Status (1)
| Country | Link |
|---|---|
| NL (1) | NL2031127B1 (en) |
-
2022
- 2022-03-01 NL NL2031127A patent/NL2031127B1/en active
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