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NL2024429B1 - Method for colouring flowers of pot plants - Google Patents

Method for colouring flowers of pot plants Download PDF

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Publication number
NL2024429B1
NL2024429B1 NL2024429A NL2024429A NL2024429B1 NL 2024429 B1 NL2024429 B1 NL 2024429B1 NL 2024429 A NL2024429 A NL 2024429A NL 2024429 A NL2024429 A NL 2024429A NL 2024429 B1 NL2024429 B1 NL 2024429B1
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NL
Netherlands
Prior art keywords
leaves
leaf tissue
opening
accessible
leaf
Prior art date
Application number
NL2024429A
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Dutch (nl)
Inventor
Christianus Gerardus De Koning Robin
Original Assignee
Gerard De Koning Bloemen B V
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Application filed by Gerard De Koning Bloemen B V filed Critical Gerard De Koning Bloemen B V
Priority to NL2024429A priority Critical patent/NL2024429B1/en
Application granted granted Critical
Publication of NL2024429B1 publication Critical patent/NL2024429B1/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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  • Life Sciences & Earth Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention concerns a method for changing the natural colour of the flower or flowers of a pot plant, said method comprising the steps of (a) providing a pot plant having one or more stems, one or more flowers, a root system surrounded by a potting medium for pot plants and 5 further having between 3 and n leaves, n being an integer of 4 or larger, (b) making at least one opening in 1 to n - 2 of the leaves such that the leaf tissue is accessible via the at least one opening, (c) providing colourant with a colour different from the natural colour of the one or more flowers, (d) exposing the leaf tissue of the 1 to n - 2 leaves made accessible in step (b) to the colourant of step (c) via the at least one opening for a period of time sufficiently long to 10 change the colour of the one or more flowers, and (e) removing the 1 to n - 2 leaves with the at least one opening from the pot plant. The invention further concerns pot plants obtainable by said method.

Description

METHOD FOR COLOURING FLOWERS OF POT PLANTS
FIELD OF THE INVENTION The invention relates to a method for colouring flowers of pot plants, in particular of plants of the orchid family (orchidaceae), and to the pot plants obtainable by said method.
BACKGROUND OF THE INVENTION The invention relates to the colouring of flowers of pot plants. Over the years, many different techniques have been developed to change the natural colours of the flowers of pot plant, in particular of plants of the orchid family (orchidaceae).
One of these techniques concerns colouring the flowers of a pot plant by dissolving a dye in water, followed by pouring the resulting solution onto the soil around the roots of the plant such that the solution is taken up by the roots. The technique, however, has a very inefficient uptake of the dye into the roots and results in an only moderate and uneven discolouration of the flowers. Moreover, this technique leads to discoloured roots (at the exterior surface) which is unwanted from an aesthetic point of view and to soil contaminated with dye - because the major part of the dye solution sinks down along the roots into the soil - which is unwanted because it can easily result in spots and stains in the household environment where pot plants are typically applied.
WO2012/067496A 1 discloses a method for colouring flowers and/or leaves of plants, such as Anthurium, Spathiphvllum, Rosa L, Kalanchoe, Curcuma and Bromeliaceae. Said method comprises the steps of (i) physically injuring the roots of the plant such that an open fluid connection is obtained with the fluid sap flow in the xylem of the roots of the plant and (ii) contacting the physically injured roots with a colorant liquid. Like the previous technique, this method leads to discoloured roots which is unwanted from an aesthetic point of view and to soil contaminated with colorant liquid - because the major part of the colorant liquid sinks down along the roots into the soil - which is unwanted because it can easily result in spots and stains in the household environment where pot plants are typically applied.
WO2012/141579A1 discloses a method for colouring flowers of a pot plant, such as Phalaenopsis, Anthurium and Spathiphyllum. Said method comprises the steps of (1) providing an injecting means which is provided with an injection needle, (ii) filling the injecting means with a quantity of colouring liquid which is non-toxic to the pot plant, (iii) arranging in the stem of the flower a bore with a diameter corresponding to the diameter of the injection needle, (iv) inserting the injection needle into the bore, (v) injecting the stem with the quantity of colouring liquid, and (vi) removing the injection needle from the stem following the injection. The bore is typically located halfway along the overall length of the stem, and can be sealed after removing the needle.
NL2003621C concerns a method for colouring flowers of a pot plant, such as Phalaenopsis, Anthurium and Spathiphyllum. Said method comprises the steps of (1) providing a pipette, (ii) arranging in the stem of the flower a bore with a diameter corresponding to the diameter of the end of the pipette, (ii1) inserting the end of the pipette into the bore and securing the pipette towards the stem, (iv) filling the pipette with a quantity of colouring liquid which is non-toxic to the pot plant after step (i), (ii) or (iii), and (v) removing the pipette from the stem.
NL1040904C discloses a substance, such as a colourant, introduction method for plants, among which are plants of the orchid family such as Phalaenopsis orchids and Dendrobium orchids. This method comprises the steps of (i) forming a final hole into a stem of the plant, wherein the final hole is accessible via an opening in an outer surface of the stem, and wherein the final hole has a dimension in a direction parallel to a longitudinal axis of the stem which is larger than a maximum dimension of the opening in said direction parallel to the longitudinal axis of the stem and (i1) subjecting the interior of the final hole to the substance by introducing the substance through the opening. After introduction of the substance into the plant, the final hole may be closed.
Obviously, even after sealing the bore, the pot plant with discoloured flowers that results from the methods disclosed in WO2012/141579A1, NL2003621C and NL1040904C always has a visible spot typically located halfway along the overall length of the stem where the stem 1s damaged, which is aesthetically unattractive. Moreover, the methods disclosed in WO2012/141579A1, NL2003621C and NL 1040904C result in an unwanted weakening of the stem.
There is a need for novel methods for changing the natural colour of the flower or flowers of pot plants that do not have the drawbacks of the prior art.
Accordingly, it is an object of the invention to provide a method for changing the natural colour of the flower or flowers of pot plants wherein the potting plant medium surrounding the roots of the pot plant is not contaminated with colourant.
It is a further object of the invention to provide a method for changing the natural colour of the flower or flowers of pot plants wherein the roots of the plant are not damaged and which does not result in the discolouration of the roots of the plant.
It is a still further object of the invention to provide a method for changing the natural colour of the flower or flowers of pot plants wherein the stem or stems of the pot plant are not damaged.
SUMMARY OF THE INVENTION The inventors have unexpectedly found that the flowers of pot plants can be uniformly and homogeneously coloured via the leaves in an efficient way. If the leave or leaves that are used for colouring the flowers of the pot pant are subsequently removed, including for example any leave stems, the pot plants can, apart from the discolouration of the flower or flowers, not or hardly be distinguished from the original pot plants, i.e. they have no or hardly noticeable visible signs of damage. Accordingly, in a first aspect, the invention provides a method for changing the natural colour of the flower or flowers of a pot plant, said method comprising the steps of; (a) providing a pot plant having one or more stems, one or more flowers, a root system surrounded by a potting medium for pot plants and further having between 3 and # leaves, n being an integer of 4 or larger; (b) making at least one opening in 1 to # - 2 of the leaves such that the leaf tissue is accessible via the at least one opening; (c) providing colourant with a colour different from the natural colour of the one or more flowers; (d) exposing the leaf tissue of the 1 to # - 2 leaves made accessible in step (b) to the colourant of step (c) via the at least one opening for a period of time sufficiently long to change the colour of the one or more flowers; and (e) removing the 1 to 72 - 2 leaves with the at least one opening, preferably the 1 ton - 2 leaves with the at least one opening including corresponding leave stems (if present), from the pot plant.
In a second aspect, the invention provides a pot plant obtainable by the process as defined herein, wherein: ° the root system of the pot plant is not physically injured, ° the one or more stems of the pot plant are not physically injured; ° the potting medium surrounding the root system is not contaminated with the colourant; and
° the exterior surface of the root system has its natural colour. In a third aspect, the invention provides a pot plant having one or more flowers, one or more stems, at least 2 leaves and a root system surrounded by a potting medium for pot plants, wherein the one or more flowers do not have their natural colour, and wherein: ° the root system of the pot plant is not physically injured; ° the one or more stems of the pot plant are not physically injured; ° the potting medium for pot plants surrounding the root system is not contaminated with the colourant; and © the exterior surface of the root system has its natural colour.
DEFINITIONS The term ‘colouring flowers’ in the context of the present invention refers to ‘changing the natural colour of flowers’. In the context of the present invention, ‘white’ can also be a (natural) colour of the flowers.
The term ‘pot plant’ as used herein is considered to be similar to ‘potted plant’, and refers to plants that are typically cultivated and displayed in a pot, such as houseplants that are placed in residences and offices, particularly for decorative purposes.
The terms ‘eaf or ‘leaves’ as used herein do not include a ‘/eaf sten?’ unless indicated otherwise. The term ‘potting medium for pot plants’ as used herein encompasses for example ‘soil’, ‘potting soil’, ‘garden soil’, and structured potting media such as ‘potting bark’.
BRIEF DESCRIPTION OF THE FIGURES Figure 1 depicts an orchid pot plant having flowers and leaves before the colouring process, i.e. the flowers still have their natural colour. Figure 2 depicts the orchid pot plant of Figure 1 of which part of one of the leaves has been removed such that the leaf tissue is accessible. Figure 3 depicts the orchid pot plant of Figure 2 wherein a pipette with a liquid colourant therein is forced into the accessible leaf tissue to expose the leaf tissue to the liquid colourant for a sufficient time to change the natural colour of the flowers to the intended colour.
Figure 4 depicts the orchid pot plant of Figure 3, wherein the leave that was exposed to the liquid colourant is removed and wherein the natural colour of the flowers is changed into the intended colour.
5 DETAILED DESCRIPTION In a first aspect, the invention concerns a method for changing the natural colour of the flower or flowers of a pot plant, said method comprising the steps of: (a) providing a pot plant having one or more stems, one or more flowers, a root system surrounded by a potting medium for pot plants and further having between 3 and 7: leaves, # being an integer of 4 or larger; (b) making at least one opening in 1 to 7 - 2 of the leaves such that the leaf tissue is accessible via the at least one opening; (©) providing colourant with a colour different from the natural colour of the one or more flowers; (d) exposing the leaf tissue of the 1 to 7 - 2 leaves made accessible in step (b) to the colourant of step (c) via the at least one opening for a period of time sufficiently long to change the colour of the one or more flowers; and (e) removing the 1 ton - 2 leaves with the at least one opening, preferably the 1 to 7 - 2 leaves with the at least one opening including corresponding leave stems (if present), from the pot plant.
In a second aspect, the invention provides a pot plant obtainable by the process as defined herein, wherein: ° the root system of the pot plant is not physically injured; e= the one or more stems of the pot plant are not physically injured; ° the potting medium for pot plants surrounding the root system is not contaminated with the colourant; and ° the exterior surface of the root system has its natural colour.
A physically injured root system refers to a root system that has been damaged, such as for example by bruising, breaking, cutting, sawing or drilling.
A physically injured stem refers to a stem that has been damaged, such as for example by bruising, breaking, cutting, sawing or drilling. Hence, a stem that has been cut away (almost) up to the root system is also considered a physically injured stem.
In a third aspect, the invention provides a pot plant having one or more flowers, one or more stems, at least 2 leaves and a root system surrounded by a potting medium for pot plants, wherein the one or more flowers do not have their natural colour, and wherein: ° the root system of the pot plant is not physically injured, ° the one or more stems of the pot plant are not physically injured; © the potting medium for pot plants surrounding the root system is not contaminated with the colourant; and ° the exterior surface of the root system has its natural colour. Pot plant In step (a), a pot plant having one or more stems, one or more flowers, a root system surrounded by a potting medium for pot plants and further having between 3 and 7: leaves is provided. Although in step (a) a pot plant is provided, the process as defined herein does not necessarily require the presence of a pot. The process can also be performed when the pot plant 1s not present in a pot, although the presence of a pot is preferred. Similarly, the process as defined herein does not necessarily require the presence of the potting medium for pot plants. The process can also be performed when the root system is, temporarily, not surrounded by a potting medium for pot plants. In an embodiment, the potting medium for pot plants is chosen from soil, potting soil, garden soil, soil structuring agents, potting bark and combinations thereof. In a preferred embodiment, the one or more flowers of the pot plant provided in step (a) have a natural colour with high saturation and high brightness. In another preferred embodiment, the one or more flowers of the pot plant provided in step (a) have a natural colour chosen from the group consisting of white, off-white, pearl, ivory and yellow. In a very preferred embodiment, the one or more flowers of the pot plant provided in step (a) have a natural colour chosen from white and off-white. Most preferably, the natural colour is white. The pot plant is preferably chosen from the group consisting of the orchid family (orchidaceae), Anthurium and Spathiphyllum.
In a more preferred embodiment, the pot plant is chosen from plants of the orchid family (orchidaceae), even more preferably chosen from Phalaenopsis orchids and Dendrobium orchids, most preferably from Phalaenopsis orchids.
The pot plant provided in step (a) has between 3 and » leaves, n being an integer of 4 or larger. In embodiments, the pot plant provided in step (a) has between 3 and # = 500 leaves, preferably between 4 and #2 = 100 leaves, more preferably between 5 and 1 = 75 leaves, still more preferably between 6 and 7? = 50 leaves, yet more preferably between 7 and 73 = 40 leaves.
The pot plant, i.e. the pot plant that results from the method as defined herein or that is obtainable using the method as defined herein, has between 2 and 1 — 1 leaves, such as between 2 and 499 leaves, preferably between 3 and # = 99 leaves, more preferably between 4 and n = 74 leaves, still more preferably between 5 and n = 49 leaves, yet more preferably between 6 and # = 39 leaves.
Making leaf tissue accessible In step (b) at least one opening in 1 to # - 2 of the leaves is made such that the leaf tissue of said 1 to 7 - 2 leaves is accessible via the at least one opening in those leaves. As will be appreciated by those skilled in the art, the wording ‘the leaf tissue of the leaves is accessible via the at least one opening in those leaves’, Is to be construed as ‘the leaf tissue of the leaves is accessible to contacting with the colourant as defined herein via the at least one opening in those leaves’.
Step (b) throughout the application can also be worded as ‘making at least one opening inx = 1 ton - 2 of the leaves such that the leaf tissue of the x leaves is accessible via the at least one opening, wherein x is an integer’. In other words, at least one opening is made in x leaves. The wording ‘the 1 to 7 - 2 leaves’ thus means ‘the x leaves’.
Since, the number of leaves of the pot plant provided in step (a) is between 3 and #, n being an integer of 4 or larger, the number of leaves of which the leaf tissue is not made accessible is between 2 and # - 1. This means that at least two leaves remain unatfected.
In a preferred embodiment, one opening is made in step (b) in the 1 to # - 2 leaves. In another preferred embodiment, at least one opening is made in step (b) in 1 or 2, preferably only in 1 leaf. In a very preferred embodiment, one opening is made in step (b) in only 1 leaf such that the leaf tissue of only 1 leaf is made accessible.
The 1 to n - 2 leaves wherein at least one opening is made are preferably located somewhere in the middle of the 3 to # leaves distributed in a vertical direction, i.e. the 1 to #- 2 leaves are preferably chosen somewhere in the middle of the 3 to 7 leaves in a direction parallel to the one or more stems of the pot plant.
The at least one opening in the 1 to 1 - 2 leaves can be introduced using different techniques. Techniques that can be advantageously used in step (b) can be independently chosen for every opening in every individual leaf from the group consisting of: (i) removing part of the leaf by cutting with a knife; (i) removing part of the leaf by cutting with a pair of scissors; (11) drilling or cutting a hole in the leaf with a hand drill or drilling machine; (iv) inserting one end of a pipette into the leaf tissue; (v) inserting one end of a straw into the leaf tissue; (vi) inserting one end of a needle into the leaf tissue, wherein the needle is optionally equipped with a syringe; (vil) making accessible the leaf tissue using (1) followed by subjecting the accessible leaf tissue to any one of (iii) to (vii); and (vii) making accessible the leaf tissue using (11) followed by subjecting the accessible leaf tissue to any one of (iii) to (vii).
In a preferred embodiment, the at least one opening in the 1 to 7: - 2 leaves are introduced in step (b) by removing part of the leaf by cutting with a knife or by removing part of the leaf by cutting with a pair of scissors, preferably by cutting with a knife. If part of the leaf is removed by cutting with a knife or by cutting with a pair of scissors, the remaining part of the leave preferably extends about 2 to 4 cm from the stem/roots. In doing so, the leaf is cut there where its thickness is highest such that a pipette, straw of needled can be easily inserted into the accessible leaf tissue. In another preferred embodiment, the at least one opening in the 1 to # - 2 leaves are introduced in step (b) by removing part of the leaf by cutting with a knife or by removing part of the leaf by cutting with a pair of scissors, preferably by cutting with a knife, followed by drilling or cutting a hole in the accessible leaf tissue with a hand drill or drilling machine.
Exposing the leaf tissue to the colourant The leaf tissue of the 1 to 7 - 2 leaves made accessible in step (b) is exposed in step (d) to the colourant provided in step (c) via the at least one opening for a period of time sufficiently long to change the colour of the one or more flowers. It is within the skills of the artisan to choose an exposure time that results in the intended (extent of the) uniform and homogeneous colour change of the one or more flowers In a preferred embodiment, the period of time applied in step (d) is at least 12 hours, more preferably at least 14 hours, still more preferably at least 16 hours, even more preferably at least 18 hours, such as at least 19, 20, 21, 22, 23 or 24 hours.
In step (d), exposing the leaf tissue of the 1 to # - 2 leaves made accessible in step (b) to the colourant of step (c) via the at least one opening can be performed using different techniques depending on the type of opening that has been made in step (b) and/or on the way in which the opening has been made (b).
In a first embodiment, the at least one opening in the 1 to # - 2 leaves are introduced in step (b) by removing part of the leaf by cutting with a knife or by removing part of the leaf by cutting with a pair of scissors, preferably by cutting with a knife, followed by: (1) inserting one end of a pipette in the accessible leaf tissue; (i) inserting one end of a straw in the accessible leaf tissue; or (ii) inserting one end of a needle in the accessible leaf tissue, wherein the needle is equipped with a syringe, wherein the pipette, straw of syringe is filled with the colourant before insertion or is filled with the colourant after insertion to expose the leaf tissue to the colourant.
In a second embodiment, the at least one opening in the 1 to 7 - 2 leaves are introduced in step (b) by removing part of the leaf by cutting with a knife or by removing part of the leaf by cutting with a pair of scissors, preferably by cutting with a knife, followed by drilling or cutting a hole in the accessible leaf tissue with a hand drill or drilling machine, further followed by: (1) inserting one end of a pipette in the hole in the accessible leaf tissue; (ij) inserting one end of a straw in the hole in the accessible leaf tissue; or (ii) inserting one end of a needle in the hole in the accessible leaf tissue, wherein the needle is equipped with a syringe, wherein the pipette, straw of syringe is filled with the colourant before insertion or is filled with the colourant after insertion to expose the leaf tissue to the colourant.
This embodiment is advantageous because the drilling or cutting in the accessible leaf tissue actually removes plant material/plant tissue, thereby creating a hole that is already suitable for receiving one end of a pipette, straw of needle.
In a preferred embodiment, the diameter of the hole substantially corresponds to the diameter of the end of the pipette, straw of needle that is inserted into the hole.
In a third embodiment, the at least one opening in the 1 to # - 2 leaves are introduced in step (b) by inserting one end of a pipette in the leaf tissue or by inserting one end of a straw in the leaf tissue, followed by filling the pipette or straw with the colourant to expose the leaf tissue to the colourant.
In a fourth embodiment, the at least one opening in the 1 to # - 2 leaves are introduced in step (b) by inserting one end of a needle in the leaf tissue, followed by attaching a syringe to the other end of the needle, followed by filling the syringe with the colourant to expose the leaf tissue to the colourant.
The use of a syringe can be advantageous because the colourant, when liquid, can then be forced into the leaf tissue.
One end of the pipette, straw or needle is preferably inserted into the accessible leaf tissue at an angle with the vertical direction, i.e. at an angle with the direction parallel to the one or more stems, such that the opening of the pipette, straw or needle not inserted into the accessible leaf tissue points upwards. This configuration prevents leaking of the colourant in case the pipette, straw of needle is not closed. Moreover, this configuration improves the contact of the colourant with the leaf tissue. The pipette, straw or needle may be secured in the intended configuration by supporting means. Typically, however, no further support is needed. Colourant In step (d), the at least one opening in the 1 to 72 - 2 leaves are exposed to colourant.
As will be appreciated by those skilled in the art, the colourant is chosen from colourants that are (at least in the amounts applied to the plant) non-toxic to the pot plant. Moreover, the colourant is chosen from colourants that can be taken up by the sap flow of the pot plant when they are contacted with the sap flow. The pot plant may be supplied with water before step (d).
In a preferred embodiment, the colourant is a liquid colourant, more preferably an aqueous solution of a dye. The term ‘aqueous solution’ means that the solvent of the dye comprises or consists of water. The solvent can however also comprise small amounts of co- solvents for the dye and one or more salts. In an embodiment, the colourant is a liquid colourant being an aqueous solution of a dye, optionally comprising one or more co-solvents for the dye and optionally comprising one or more salts.
In an embodiment, the colourant is a dye in powder form. Colouring the one or more flowers of the pot plant with a colourant being a dye in powder form 1s less preferred than colouring the one or more flowers with a liquid colourant because the dye in powder form is less easily taken up by the sap streams of the leaves. If the one or more flowers of the pot plant are coloured with a colourant being a dye in powder form, it is preferred that the pot plant is supplied with water before step (d).
The colourant as defined hereinbefore can also be a mixture of two or more colourants, such as a mixture of two or more dyes.
In an embodiment, the leaf tissue of two or more openings in a single leaf is exposed to different colourants in step (c). In another embodiment, the leaf tissue of openings in different leaves is exposed to different colourants in step (c).
Amount of colourant applied It is within the skills of the artisan to choose a suitable amount of colourant to contact the accessible leaf tissue via an opening. In an embodiment wherein a liquid colourant, such as a concentrated aqueous solution of one or more dyes, is applied, the amount typically applied per opening is between 0.5 and 4 ml, preferably between 0.7 and 2 ml. In an embodiment wherein a colourant that is a dye in powder form 1s applied, the amount typically applied per opening is between 0.2 and 2 g.
Specific preferred embodiments A preferred embodiment concerns a method for changing the natural colour of the flower or flowers of a pot plant, said method comprising the steps of: (a) providing a pot plant having one or more stems, one or more flowers, a root system surrounded by a potting medium for pot plants and further having between 6 and 50 leaves; (b) making one opening in 1 or 2 of the leaves such that the leaf tissue is accessible via the opening; (c) providing a liquid colourant with a colour different from the natural colour of the one or more flowers; (d) exposing the leaf tissue of the 1 or 2 leaves made accessible in step (b) to the liquid colourant of step (c) via the opening for at least 12 hours; and (e) removing the 1 or 2 leaves with the opening, preferably the 1 or 2 leaves with the opening including corresponding leave stems, from the pot plant.
A more preferred embodiment concerns a method for changing the natural colour of the flower or flowers of a pot plant, preferably of a Phalaenopsis orchid, said method comprising the steps of: (a) providing a pot plant having one or more stems, one or more flowers, a root system surrounded by a potting medium for pot plants and further having between 6 and 50 leaves; (b) making one opening in 1 of the leaves such that the leaf tissue is accessible via the opening, preferably by removing part of the leaf by cutting with a knife or by removing part of the leaf by cutting with a pair of scissors;
(c) providing an aqueous solution of a dye with a colour different from the natural colour of the one or more flowers; (d) exposing the leaf tissue of the 1 leaf made accessible in step (b) to the aqueous solution of the dye of step (c) via the opening for at least 12 hours, preferably by: (1) inserting one end of a pipette in the accessible leaf tissue; (ij) inserting one end of a straw in the accessible leaf tissue; or (iv) inserting one end of a needle in the accessible leaf tissue, wherein the needle is equipped with a syringe, at an angle with the vertical direction such that the opening of the pipette, straw or needle not inserted into the accessible leaf tissue points upwards, wherein the pipette, straw or syringe is filled with the aqueous solution of the dye before insertion of the pipette, straw or syringe or is filled with the aqueous solution of the dye after insertion of the pipette, straw or syringe to expose the leaf tissue to the aqueous solution of the dye; and (e) removing the 1 leaf with the opening, preferably the 1 leaf with the opening including a corresponding leave stem, from the pot plant.
Thus, the invention has been described by reference to certain embodiments discussed above. It will be recognized that these embodiments are susceptible to various modifications and alternative forms well known to those of skill in the art.
Furthermore, for a proper understanding of this document and its claims, it is to be understood that the verb ‘to comprise’ and its conjugations are used in its non-limiting sense to mean that items following the word are included, but items not specifically mentioned are not excluded. In addition, reference to an element by the indefinite article ‘a’ or ‘an’ does not exclude the possibility that more than one of the element is present, unless the context clearly requires that there be one and only one of the elements. The indefinite article ‘a’ or ‘an’ thus usually means ‘at least one’ or ‘one or more’.
EXAMPLES Colouring experiments were performed as follows. In a first step, a number of 10 Phalaenopsis pot plants having white flowers (natural colour) were provided. The Phalaenopsis pot plants all had a minimum number of six leaves and at least two stems with flowers.
As an example, Figure 1 depicts a Phalaenopsis pot plant (1) having a single stem (5) with flowers (2) and seven leaves (3) before the colouring process, i.e. the flowers (2) still have their natural colour. In Figure 1, the stem (5) is attached via a clip (4) to a support (7). The Phalaenopsis pot plant is provided with a pot (6) containing the root system and surrounding potting medium for pot plants (not shown).
The following procedure was followed for every individual Phalaenopsis pot plant.
In a second step, part of one of the leaves was removed using a knife such that the remaining part on the leaf extended about 2 to 4 cm from the stem/roots. In doing so, the leaf 1s cut there where its thickness is highest such that a pipette can be easily inserted into the accessible leaf tissue.
This leaf of which part is removed was located somewhere in the middle of the leaves distributed in a vertical direction, i.e. a leaf was chosen somewhere in the middle of the leaves in a direction parallel to the stems of the pot plant.
Figure 2 depicts the result after the second process step. Leaf tissue (8) was made accessible. The maximum thickness of the leaf where the leaf was cut is indicated with ‘d".
In a third step, a pipette (9) was inserted into the accessible leaf tissue (8). The pipette (9) had a capacity of about 4 ml and had two openings, one opening with a small diameter for releasing content and one opening with a larger diameter for filling the pipette (9) with content. Inserting the pipette (9) into the accessible leaf tissue (8) means that the end of the opening with the smaller diameter is forced into the leaf tissue such that it remains in the same position without further support. The pipette was inserted into the accessible leaf tissue (8) at an angle with the vertical direction, such that the opening of the pipette (9) with the larger diameter pointed upwards. The pipette (9) was subsequently filled with about 1.5 ml of a liquid blue colourant (10) (aqueous solution of a water-soluble blue dye which is non-toxic to the Phalaenopsis pot plant). Thus, the pot plant (1) was exposed to the colourant for a period of at about 12 hours.
After this period of time, all flowers had turned blue.
See Figure 3 for the result of the third process step.
In a fourth step, the pipette was removed.
Subsequently, the remainder of the leaf that was exposed to the liquid colourant was removed.
The resulting pot plant (1) had blue flowers (2a) and no signs of damage to the flowers, stems and leaves.
See Figure 4 for the result of the fourth process step.

Claims (15)

ConclusiesConclusions 1. Werkwijze voor het veranderen van de natuurlijke kleur van de bloem of de bloemen van een potplant, waarbij de werkwijze de volgende stappen omvat: (a) het verschaffen van een potplant met één of meer stammen, één of meer bloemen, een wortelsysteem omgeven door een potmedium voor potplanten en verder hebbende tussen 3 en # bladeren, waarbij 7: een geheel getal van 4 of groter is; (b) het maken van tenminste één opening in 1 tot #2 - 2 van de bladeren op zodanige wijze dat het bladweefsel toegankelijk is via de ten minste één opening; (c) het verschaffen van kleurmiddel met een kleur verschillend van de natuurlijke kleur van de één of meer bloemen; (d) het blootstellen van het bladweefsel van de 1 tot #2 — 2 bladeren dat toegankelijk is gemaakt in stap (b) aan het kleurmiddel uit stap (c) via de ten minste één opening voor een tijdsperiode welke voldoende lang is om de kleur van de één of meer bloemen te wijzigen, en (e) het verwijderen van de 1 tot 72 — 2 bladeren met de ten minste één opening, bij voorkeur de 1 tot #7 — 2 bladeren met de ten minste één opening omvattende bijbehorende bladstelen, van de potplant.A method for changing the natural color of the flower or flowers of a potted plant, the method comprising the steps of: (a) providing a potted plant having one or more stems, one or more flowers, surrounding a root system through a potting medium for potted plants and further having between 3 and # leaves, wherein 7: is an integer of 4 or greater; (b) making at least one opening in 1 to #2 - 2 of the leaves such that the leaf tissue is accessible through the at least one opening; (c) providing colorant having a color different from the natural color of the one or more flowers; (d) exposing the leaf tissue of the 1 to #2 — 2 leaves made accessible in step (b) to the stain from step (c) through the at least one opening for a period of time sufficient for the color to of the one or more flowers, and (e) removing the 1 to 72 - 2 leaves with the at least one opening, preferably the 1 to #7 - 2 leaves with the at least one opening including associated petioles, of the pot plant. 2. Werkwijze volgens conclusie 1, waarbij de tijdsperiode toegepast in stap (d) ten minste 12 uur bedraagt, bij voorkeur ten minste 14 uur, met sterkere voorkeur ten minste 16 uur, met nog sterkere voorkeur ten minste 18 uur, zoals ten minste 19, 20, 21, 22, 23 or 24 uur.A method according to claim 1, wherein the period of time used in step (d) is at least 12 hours, preferably at least 14 hours, more preferably at least 16 hours, even more preferably at least 18 hours, such as at least 19 , 20, 21, 22, 23 or 24 hours. 3. Werkwijze volgens conclusie 1 of 2, waarbij de ten minste één opening in de 1 tot # — 2 bladeren in stap (b) gemaakt worden met een techniek onafhankelijk gekozen voor elke opening en elk individueel blad uit de groep bestaande uit: (i) het verwijderen van een deel van het blad door te snijden met een mes; (ii) het verwijderen van een deel van het blad door te knippen met een schaar; (ii) het boren of snijden van een gat in het blad met een handboor of een boormachine; (iv) het inbrengen van een uiteinde van een pipet in het bladweefsel, (v) het inbrengen van een uiteinde van een rietje in het bladweefsel; (vi) het inbrengen van een uiteinde van een naald in het bladweefsel, waarbij de naald optioneel uitgerust is met een spuit;The method of claim 1 or 2, wherein the at least one opening in the 1 to # - 2 leaves in step (b) is made by a technique selected independently for each opening and each individual leaf from the group consisting of: (i ) removing part of the blade by cutting with a knife; (ii) removing part of the leaf by cutting with scissors; (ii) drilling or cutting a hole in the blade with a hand drill or power drill; (iv) inserting a pipette tip into the leaf tissue, (v) inserting a straw tip into the leaf tissue; (vi) inserting a tip of a needle into the leaf tissue, the needle optionally being equipped with a syringe; (vii) het toegankelijk maken van het bladweefsel gebruikmakend van (i) gevolgd door het onderwerpen van het toegankelijke bladweefsel aan één van (iii) tot (vii); en (viii) het toegankelijk maken van het bladweefsel gebruikmakend van (ii) gevolgd door het onderwerpen van het toegankelijke bladweefsel aan één van (iii) tot (vii).(vii) making the leaf tissue accessible using (i) followed by subjecting the accessible leaf tissue to one of (iii) to (vii); and (viii) making the leaf tissue accessible using (ii) followed by subjecting the accessible leaf tissue to one of (iii) to (vii). 4. Werkwijze volgens één van de conclusies 1 - 3, waarbij de ten minste één opening in de 1 tot 72 — 2 bladeren in stap (b) geïntroduceerd worden door het verwijderen van een deel van het blad door het snijden met een mes of door het verwijderen van een deel van het blad door te knippen met een schaar, gevolgd door: (i) het inbrengen van een uiteinde van een pipet in het toegankelijke bladweefsel, (ii) het inbrengen van een uiteinde van een rietje in het toegankelijke bladweefsel; of (ii) het inbrengen van een uiteinde van een naald in het toegankelijke bladweefsel, waarbij de naald uitgerust is met een spuit, waarbij de pipet, het rietje of de spuit gevuld is met het kleurmiddel vóór het inbrengen of gevuld is met het kleurmiddel na het inbrengen om het bladweefsel in stap (d) bloot te stellen aan het kleurmiddel.A method according to any one of claims 1 to 3, wherein the at least one opening in the 1 to 72-2 leaves in step (b) is introduced by removing a portion of the leaf by cutting with a knife or by cutting removing a portion of the leaf by cutting with scissors, followed by: (i) inserting a pipette tip into the accessible leaf tissue, (ii) inserting a straw tip into the accessible leaf tissue; or (ii) inserting a tip of a needle into the accessible leaf tissue, the needle being fitted with a syringe, the pipette, straw or syringe being filled with the colorant before insertion or filled with the colorant after introducing to expose the leaf tissue in step (d) to the colorant. 5. Werkwijze volgens één van de conclusies 1-3, waarbij de ten minste één opening in de 1 tot n — 2 bladeren in stap (b) geïntroduceerd zijn door het verwijderen van een deel van het blad door het snijden met een mes of door het verwijderen van een deel van het blad door te knippen met een schaar, gevolgd door het boren of snijden van een gat in het toegankelijke bladweefsel met een handboor of een boormachine, verder gevolgd door: (1) hetinbrengen van een uiteinde van een pipet in het toegankelijke bladweefsel, (i) het inbrengen van een uiteinde van een rietje in het toegankelijke bladweefsel; of (ii) het inbrengen van een uiteinde van een naald in het toegankelijke bladweefsel, waarbij de naald uitgerust is met een spuit, waarbij de pipet, het rietje of de spuit gevuld is met het kleurmiddel vóór het inbrengen of gevuld is met het kleurmiddel na het inbrengen om het bladweefsel in stap (d) bloot te stellen aan het kleurmiddel.A method according to any one of claims 1 to 3, wherein the at least one opening in the 1 to n - 2 leaves is introduced in step (b) by removing a portion of the leaf by cutting with a knife or by cutting removing a portion of the leaf by cutting with scissors, followed by drilling or cutting a hole in the accessible leaf tissue with a hand drill or power drill, followed by: (1) inserting a pipette tip into the accessible leaf tissue, (i) inserting one end of a straw into the accessible leaf tissue; or (ii) inserting a tip of a needle into the accessible leaf tissue, the needle being fitted with a syringe, the pipette, straw or syringe being filled with the colorant before insertion or filled with the colorant after introducing to expose the leaf tissue in step (d) to the colorant. 6. Werkwijze volgens één van de conclusies 1-5, waarbij de potplant verschaft in stap (a) tussen 3 en # = 500 bladeren heeft, bij voorkeur tussen 4 en 7: = 100 bladeren, bij sterkere voorkeur tussen 5 en n = 75 bladeren, met nog sterkere voorkeur tussen 6 en # = 50 bladeren.A method according to any one of claims 1-5, wherein the potted plant provided in step (a) has between 3 and # = 500 leaves, preferably between 4 and 7: = 100 leaves, more preferably between 5 and n = 75 leaves, with an even stronger preference between 6 and # = 50 leaves. 7. Werkwijze volgens één van de conclusies 1-6, waarbij in stap (b) één opening gemaakt wordt in de 1 tot # — 2 bladeren.A method according to any one of claims 1-6, wherein in step (b) one opening is made in the 1 to #-2 leaves. 8. Werkwijze volgens één van de conclusies 1-7, waarbij in stap (b) één opening wordt gemaakt in één van de bladeren.A method according to any one of claims 1-7, wherein in step (b) one opening is made in one of the leaves. 9. Werkwijze volgens één van de conclusies 1-8, waarbij het kleurmiddel verschaft in stap (c) een vloeibaar kleurmiddel is, bij voorkeur een waterige oplossing van één of meer kleurstoffen.A method according to any one of claims 1-8, wherein the colorant provided in step (c) is a liquid colorant, preferably an aqueous solution of one or more colorants. 10. Werkwijze volgens conclusie 1, waarbij de werkwijze de volgende stappen omvat: (a) het verschaffen van de potplant met één of meer stammen, één of meer bloemen, een wortelsysteem omgeven door een potmedium voor potplanten en verder hebbende tussen 6 en 50 bladeren; (b) het maken van één opening in 1 of 2 van de bladeren op zodanige wijze dat het bladweefsel toegankelijk is via de opening; (c) het verschaffen van een vloeibaar kleurmiddel met een kleur verschillend van de natuurlijke kleur van de één of meer bloemen; (d) het blootstellen van het bladweefsel van de 1 of 2 bladeren dat toegankelijk is gemaakt in stap (b) aan het vloeibare kleurmiddel uit stap (c) via de opening voor ten minste 12 uur; en (e) het verwijderen van de 1 of 2 bladeren met de opening, bij voorkeur de 1 of 2 bladeren met de opening omvattende bijbehorende bladstelen, van de potplant.The method of claim 1, wherein the method comprises the steps of: (a) providing the potted plant having one or more stems, one or more flowers, a root system surrounded by a potting medium for potted plants and further having between 6 and 50 leaves ; (b) making one opening in 1 or 2 of the leaves such that the leaf tissue is accessible through the opening; (c) providing a liquid colorant having a color different from the natural color of the one or more flowers; (d) exposing the leaf tissue of the 1 or 2 leaves made accessible in step (b) to the liquid colorant from step (c) through the opening for at least 12 hours; and (e) removing the 1 or 2 leaves with the aperture, preferably the 1 or 2 leaves with the aperture including associated petioles, from the potted plant. 11. Werkwijze volgens conclusie 1, waarbij de werkwijze de volgende stappen omvat: (a) het verschaffen van de potplant met één of meer stammen, één of meer bloemen, een wortelsysteem omgeven door een potmedium voor potplanten en verder hebbende tussen 6 en 50 bladeren; (b) het maken van één opening in 1 van de bladeren op zodanige wijze dat het bladweefsel toegankelijk is via de opening, bij voorkeur door het verwijderen van een deel van het blad door het snijden met een mes of door het verwijderen van een deel van het blad door te knippen met een schaar; (c) het verschaffen van een waterige oplossing van een kleurstof met een kleur verschillend van de natuurlijke kleur van de één of meer bloemen;The method of claim 1, wherein the method comprises the steps of: (a) providing the potted plant having one or more stems, one or more flowers, a root system surrounded by a potting medium for potted plants and further having between 6 and 50 leaves ; (b) making one opening in 1 of the leaves in such a way that the leaf tissue is accessible through the opening, preferably by removing part of the leaf by cutting with a knife or by removing part of the leaf cutting the leaf with scissors; (c) providing an aqueous solution of a dye having a color different from the natural color of the one or more flowers; (d) het blootstellen van het bladweefsel van het ene blad dat toegankelijk is gemaakt in stap (b) aan de waterige oplossing van de kleurstof uit stap (c) via de opening voor ten minste 12 uur, bij voorkeur door: (1) het inbrengen van een uiteinde van een pipet in het toegankelijke bladweefsel, (i) het inbrengen van een uiteinde van een rietje in het toegankelijke bladweefsel, of (11) het inbrengen van een uiteinde van een naald in het toegankelijke bladweefsel, waarbij de naald uitgerust is met een spuit; in een hoek met de verticale richting zodanig dat de opening van de pipet, het rietje of de naald welke niet ingebracht is in het toegankelijke bladweefsel naar boven wijst, waarbij de pipet, het rietje of de spuit gevuld is met de waterige oplossing van de kleurstof vóór het inbrengen of gevuld is met de waterige oplossing van de kleurstof na het inbrengen om het bladweefsel in stap (d) bloot te stellen aan de waterige oplossing van de kleurstof, en (e) het verwijderen van het ene blad met de opening, bij voorkeur het ene blad met de opening omvattende een bijbehorende bladsteel, van de potplant.(d) exposing the leaf tissue of the one leaf made accessible in step (b) to the aqueous solution of the dye from step (c) through the opening for at least 12 hours, preferably by: (1) inserting a tip of a pipette into the accessible leaf tissue, (i) inserting a tip of a straw into the accessible leaf tissue, or (11) inserting a tip of a needle into the accessible leaf tissue, the needle being fitted with a syringe; at an angle to the vertical such that the opening of the pipette, straw or needle not inserted into the accessible leaf tissue points upwards, the pipette, straw or syringe being filled with the aqueous solution of the dye before insertion or filled with the aqueous solution of the dye after insertion to expose the leaf tissue in step (d) to the aqueous solution of the dye, and (e) removing the one leaf having the aperture, at preferably the one leaf with the opening comprising an associated petiole, of the pot plant. 12. Werkwijze volgens één van de conclusies 1-11, waarbij de één of meer bloemen van de potplant verschaft in stap (a) een natuurlijke kleur hebben gekozen uit de groep bestaande uit wit, gebroken wit, parelwit, ivoorwit en geel, bij voorkeur gekozen uit wit en gebroken wit, waarbij de natuurlijke kleur bij sterkere voorkeur wit is.A method according to any one of claims 1-11, wherein the one or more flowers of the potted plant provided in step (a) have a natural color selected from the group consisting of white, off-white, pearl white, ivory white and yellow, preferably selected from white and off-white, with the more preferred natural color being white. 13. Werkwijze volgens één van de conclusies 1-12, waarbij de potplant gekozen wordt uit de groep bestaande uit de orchideeënfamilie (orchidaceae), Anthurium en Spathiphyllum, bij voorkeur gekozen uit planten van de orchideeënfamilie (orchidaceae), bij sterkere voorkeur gekozen uit Phalaenopsis orchideeën en Dendrobium orchideeën, met de meeste voorkeur uit Phalaenopsis orchideeën.A method according to any one of claims 1-12, wherein the potted plant is selected from the group consisting of the orchid family (orchidaceae), Anthurium and Spathiphyllum, preferably selected from plants of the orchid family (orchidaceae), more preferably selected from Phalaenopsis orchids and Dendrobium orchids, most preferably from Phalaenopsis orchids. 14. Potplant verkrijgbaar via de werkwijze volgens één van de conclusies 1-13, waarbij: ° het wortelsysteem van de potplant niet fysiek beschadigd is; ° de één of meer stammen van de potplant niet fysiek beschadigd zijn; ° het potmedium voor potplanten dat het wortelsysteem omgeeft niet met het kleurmiddel gecontamineerd is; enA potted plant obtainable by the method according to any one of claims 1-13, wherein: ° the root system of the potted plant is not physically damaged; ° the one or more stems of the pot plant are not physically damaged; ° the potting medium for pot plants surrounding the root system is not contaminated with the coloring agent; and ° het buitenoppervlak van het worstelsysteem zijn natuurlijke kleur heeft.° the outer surface of the wrestling system has its natural color. 15. Potplant met één of meer bloemen, één of meer stammen, ten minste 2 bladeren en een wortelsysteem omringd door aarde, waarbij de één of meer bloemen niet hun natuurlijke kleur hebben, verkregen via de werkwijze volgens één van de conclusies 1-13, waarbij: ° het wortelsysteem van de potplant niet fysiek beschadigd is; ° de één of meer stammen van de potplant niet fysiek beschadigd zijn; ° het potmedium voor potplanten dat het wortelsysteem omgeeft niet met het kleurmiddel gecontamineerd is; en ° het buitenoppervlak van het worstelsysteem zijn natuurlijke kleur heeft.A potted plant with one or more flowers, one or more stems, at least 2 leaves and a root system surrounded by soil, wherein the one or more flowers do not have their natural color, obtained by the method according to any one of claims 1-13, where: ° the root system of the pot plant is not physically damaged; ° the one or more stems of the pot plant are not physically damaged; ° the potting medium for pot plants surrounding the root system is not contaminated with the coloring agent; and ° the outer surface of the wrestling system has its natural color.
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NL1030581C2 (en) * 2005-11-25 2006-05-10 Joost Petrus Jacobus Bakker Increasing the number of inflorescences in a Phalaenopsis orchid comprises administering a cytokinin to the orchid and exposing it to a cold period
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WO2010085082A2 (en) * 2009-01-23 2010-07-29 Kim Jae-Jong Dye storage tube for storing dyes for flower petals, and method for dyeing flower petals using same
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL1030581C2 (en) * 2005-11-25 2006-05-10 Joost Petrus Jacobus Bakker Increasing the number of inflorescences in a Phalaenopsis orchid comprises administering a cytokinin to the orchid and exposing it to a cold period
WO2009143649A1 (en) * 2008-05-27 2009-12-03 安龙生物科技股份有限公司 Method to alter flower and leaf color
WO2010085082A2 (en) * 2009-01-23 2010-07-29 Kim Jae-Jong Dye storage tube for storing dyes for flower petals, and method for dyeing flower petals using same
NL2003621C2 (en) 2009-10-12 2011-04-13 Logico B V METHOD FOR COLORING A FLOWER ON A POTPLANT AND POTPLANT WITH COLORED FLOWER.
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