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MXPA02002507A - Deteccion de alteraciones en un gen por pcr de largo alcance mediante el uso de elementos moviles humanos. - Google Patents

Deteccion de alteraciones en un gen por pcr de largo alcance mediante el uso de elementos moviles humanos.

Info

Publication number
MXPA02002507A
MXPA02002507A MXPA02002507A MXPA02002507A MXPA02002507A MX PA02002507 A MXPA02002507 A MX PA02002507A MX PA02002507 A MXPA02002507 A MX PA02002507A MX PA02002507 A MXPA02002507 A MX PA02002507A MX PA02002507 A MXPA02002507 A MX PA02002507A
Authority
MX
Mexico
Prior art keywords
gene
alteration
interest
test sample
genomic dna
Prior art date
Application number
MXPA02002507A
Other languages
English (en)
Inventor
Thorarinn Blondal
Original Assignee
Decode Genetics Ehf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/470,673 external-priority patent/US6225093B1/en
Application filed by Decode Genetics Ehf filed Critical Decode Genetics Ehf
Publication of MXPA02002507A publication Critical patent/MXPA02002507A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se describen metodos para detectar una alteracion en un gen de interes, tal como un retraso en el gen C4A (por ejemplo, para detectar el C4AQ0), realizando la amplificacion de reaccion en cadena de polimerasa en un alto indice sobre una muestra de prueba que comprende ADN genomico. Los metodos amplifican el ADN objetivo que comprende todo o una porcion de un elemento movil humano (por ejemplo, un injerto retroviral en el intron 9 del gen C4A), mediante el uso de cebadores designados de modo que se formen los productos PCR solo si la muestra de prueba comprende ADN genomico que incluye la alteracion en el gen de interes; de manera alternativa, los metodos amplifican el ADN objetivo usando cebadores designados de tal modo que los productos PCR se formen solo si la muestra de prueba comprende ADN genomico que no incluye la alteracion en el gen de interes. Alternativamente, los cebadores son designados de tal modo que los productos PCR tengan tamanos diferentes de manera detectable, deperdiendo si la muestra de prueba comprende ADN genomico que incluye la alteracion del gen de interes. Los metodos pueden ser utiles para identificar si un individuo tiene el riesgo de una enfermedad o estado asociado con la alteracion en el gen de interes, en tanto la presencia de la alteracion se correlaciona con el riesgo de la enfermedad.
MXPA02002507A 1999-09-07 2000-09-06 Deteccion de alteraciones en un gen por pcr de largo alcance mediante el uso de elementos moviles humanos. MXPA02002507A (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US39124499A 1999-09-07 1999-09-07
US44353999A 1999-11-19 1999-11-19
US47159899A 1999-12-23 1999-12-23
US09/470,673 US6225093B1 (en) 1999-09-07 1999-12-23 Detection of C4A deletion by long range PCR
PCT/IB2000/001283 WO2001018245A2 (en) 1999-09-07 2000-09-06 Detection of alterations in a gene by long range pcr using human mobile elements

Publications (1)

Publication Number Publication Date
MXPA02002507A true MXPA02002507A (es) 2004-09-10

Family

ID=27503328

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA02002507A MXPA02002507A (es) 1999-09-07 2000-09-06 Deteccion de alteraciones en un gen por pcr de largo alcance mediante el uso de elementos moviles humanos.

Country Status (9)

Country Link
EP (1) EP1214449A2 (es)
JP (1) JP2003508082A (es)
CN (1) CN1382221A (es)
AU (1) AU6720700A (es)
BR (1) BR0014017A (es)
CA (1) CA2383857A1 (es)
MX (1) MXPA02002507A (es)
NZ (1) NZ517641A (es)
WO (1) WO2001018245A2 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002248213A1 (en) * 2000-12-19 2002-08-19 Hospital For Special Surgery Markers for disease susceptibility and targets for therapy
TWI340168B (en) * 2003-05-07 2011-04-11 Tosoh Corp Method of detecting micrometastasis
EP2397561B1 (en) 2010-06-18 2017-08-02 Progenika Biopharma, S.A. Methods for determining the presence or absence of genetic segments
DK3102722T3 (da) 2014-02-04 2020-11-16 Jumpcode Genomics Inc Genom fraktionering
DK3110975T3 (da) * 2014-02-27 2021-10-11 Jumpcode Genomics Inc Fremgangsmåder til analyse af somatiske mobile elementer og anvendelser deraf
US10968536B2 (en) 2015-02-25 2021-04-06 Jumpcode Genomics, Inc. Methods and compositions for sequencing
US11339427B2 (en) 2016-02-12 2022-05-24 Jumpcode Genomics, Inc. Method for target specific RNA transcription of DNA sequences
CN113820499B (zh) * 2021-10-26 2024-06-25 深圳临研医学有限公司 用于诊断系统性红斑狼疮的蛋白标志物

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5096557A (en) * 1990-07-11 1992-03-17 Genetype A.G. Internal standard for electrophoretic separations

Also Published As

Publication number Publication date
BR0014017A (pt) 2002-12-31
EP1214449A2 (en) 2002-06-19
NZ517641A (en) 2004-02-27
JP2003508082A (ja) 2003-03-04
AU6720700A (en) 2001-04-10
WO2001018245A2 (en) 2001-03-15
WO2001018245A3 (en) 2002-04-18
CA2383857A1 (en) 2001-03-15
CN1382221A (zh) 2002-11-27

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