MXPA00012757A - Metodo mejorado para identificacion simultanea de arnm expresados diferencialmente y medicion de concetraciones relativas. - Google Patents
Metodo mejorado para identificacion simultanea de arnm expresados diferencialmente y medicion de concetraciones relativas.Info
- Publication number
- MXPA00012757A MXPA00012757A MXPA00012757A MXPA00012757A MXPA00012757A MX PA00012757 A MXPA00012757 A MX PA00012757A MX PA00012757 A MXPA00012757 A MX PA00012757A MX PA00012757 A MXPA00012757 A MX PA00012757A MX PA00012757 A MXPA00012757 A MX PA00012757A
- Authority
- MX
- Mexico
- Prior art keywords
- cdna
- mrna
- primers
- crna
- improved method
- Prior art date
Links
- 108020004999 messenger RNA Proteins 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 4
- 238000005259 measurement Methods 0.000 title 1
- 239000002299 complementary DNA Substances 0.000 abstract 4
- 230000006819 RNA synthesis Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 230000001575 pathological effect Effects 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
- 241001515965 unidentified phage Species 0.000 abstract 1
- 238000012800 visualization Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Un metodo mejorado para la identificacion simultanea especifica en secuencia de ARNm's en una poblacion de ARNm permite la visualizacion de casi cada ARNm expresado por un tejido como una banda distinta en un gel cuya intensidad corresponde aproximadamente a la concentracion del ARNm. En general, el metodo comprende la formacion de ADNc usando cebadores de anclaje para fijar un punto de extremo 3, produciendo insertos clonados a partir del ADNc en un vector que contiene un promotor especifico de bacteriofago para sintesis subsecuente de ARN, generando fragmentos linealizados de insertos clonados, preparando ARNc, transcribiendo ADNc a partir del ARNc usando un conjunto de cebadores 5-RT, y llevando a cabo PCR usando un cebador 3-PCR cuya secuencia es derivada del vector y un conjunto de cebadores 5-PCR que se deriva de los cebadores 5-RT usados para la transcripcion de ADNc a partir de ARNc. El metodo puede identificar cambios en la expresion de ARNm asociados con la administracion de farmacos o con condiciones fisiologicas o patologicas.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/108,099 US6096503A (en) | 1993-11-12 | 1998-06-30 | Method for simultaneous identification of differentially expresses mRNAs and measurement of relative concentrations |
| PCT/US1999/014852 WO2000000645A1 (en) | 1998-06-30 | 1999-06-30 | IMPROVED METHOD FOR SIMULTANEOUS IDENTIFICATION OF DIFFERENTIALLY EXPRESSED mRNAs AND MEASUREMENT OF RELATIVE CONCENTRATIONS |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MXPA00012757A true MXPA00012757A (es) | 2004-09-10 |
Family
ID=22320297
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MXPA00012757A MXPA00012757A (es) | 1998-06-30 | 1999-06-30 | Metodo mejorado para identificacion simultanea de arnm expresados diferencialmente y medicion de concetraciones relativas. |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6096503A (es) |
| EP (1) | EP1092044A4 (es) |
| JP (1) | JP2002519011A (es) |
| AU (1) | AU784177B2 (es) |
| CA (1) | CA2332339A1 (es) |
| MX (1) | MXPA00012757A (es) |
| NO (1) | NO20006702L (es) |
| WO (1) | WO2000000645A1 (es) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6110680A (en) * | 1993-11-12 | 2000-08-29 | The Scripps Research Institute | Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations |
| US6096503A (en) * | 1993-11-12 | 2000-08-01 | The Scripps Research Institute | Method for simultaneous identification of differentially expresses mRNAs and measurement of relative concentrations |
| US5459037A (en) * | 1993-11-12 | 1995-10-17 | The Scripps Research Institute | Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations |
| EP1007737A4 (en) * | 1997-07-25 | 2002-07-03 | Affymetrix Inc | SYSTEM FOR OBTAINING A POLYMORPHISM DATABASE $ i () |
| CA2707436C (en) | 2007-06-29 | 2014-01-28 | Epicentre Technologies Corporation | Copy dna and sense rna |
| US9650668B2 (en) | 2008-09-03 | 2017-05-16 | Nabsys 2.0 Llc | Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels |
| US8262879B2 (en) | 2008-09-03 | 2012-09-11 | Nabsys, Inc. | Devices and methods for determining the length of biopolymers and distances between probes bound thereto |
| WO2012019168A2 (en) | 2010-08-06 | 2012-02-09 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| US8715933B2 (en) | 2010-09-27 | 2014-05-06 | Nabsys, Inc. | Assay methods using nicking endonucleases |
| EP4108671B1 (en) | 2010-10-01 | 2024-11-20 | ModernaTX, Inc. | Modified nucleosides, nucleotides, and nucleic acids, and uses thereof |
| JP5998148B2 (ja) * | 2010-11-16 | 2016-09-28 | ナブシス ２．０ エルエルシー | ハイブリダイズされたプローブの相対位置を検出することによる生体分子のシークエンシングのための方法 |
| US11274341B2 (en) | 2011-02-11 | 2022-03-15 | NABsys, 2.0 LLC | Assay methods using DNA binding proteins |
| WO2012135805A2 (en) | 2011-03-31 | 2012-10-04 | modeRNA Therapeutics | Delivery and formulation of engineered nucleic acids |
| US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| CN103974724B (zh) | 2011-10-03 | 2019-08-30 | 现代泰克斯公司 | 修饰的核苷、核苷酸和核酸及其用途 |
| SG10201604896TA (en) | 2011-12-16 | 2016-08-30 | Moderna Therapeutics Inc | Modified nucleoside, nucleotide, and nucleic acid compositions |
| US9254311B2 (en) | 2012-04-02 | 2016-02-09 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of proteins |
| US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
| US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
| JP2015518704A (ja) | 2012-04-02 | 2015-07-06 | モデルナ セラピューティクス インコーポレイテッドＭｏｄｅｒｎａ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． | 膜タンパク質の産生のための修飾ポリヌクレオチド |
| HRP20220607T1 (hr) | 2012-11-26 | 2022-06-24 | Modernatx, Inc. | Terminalno modificirana rna |
| US9914966B1 (en) | 2012-12-20 | 2018-03-13 | Nabsys 2.0 Llc | Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation |
| EP2956550B1 (en) | 2013-01-18 | 2020-04-08 | Nabsys 2.0 LLC | Enhanced probe binding |
| US8980864B2 (en) | 2013-03-15 | 2015-03-17 | Moderna Therapeutics, Inc. | Compositions and methods of altering cholesterol levels |
| EP3052106A4 (en) | 2013-09-30 | 2017-07-19 | ModernaTX, Inc. | Polynucleotides encoding immune modulating polypeptides |
| EP3052521A1 (en) | 2013-10-03 | 2016-08-10 | Moderna Therapeutics, Inc. | Polynucleotides encoding low density lipoprotein receptor |
| MA46240A (fr) | 2016-09-14 | 2019-07-24 | Modernatx Inc | Compositions d'arn de haute pureté et procédés pour leur préparation |
| WO2022150717A1 (en) | 2021-01-11 | 2022-07-14 | Modernatx, Inc. | Seasonal rna influenza virus vaccines |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993018176A1 (en) * | 1992-03-11 | 1993-09-16 | Dana-Farber Cancer Institute | METHODS TO CLONE mRNA |
| US6096503A (en) * | 1993-11-12 | 2000-08-01 | The Scripps Research Institute | Method for simultaneous identification of differentially expresses mRNAs and measurement of relative concentrations |
| US5459037A (en) * | 1993-11-12 | 1995-10-17 | The Scripps Research Institute | Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations |
| EA200100490A1 (ru) * | 1998-11-04 | 2001-10-22 | Диджитал Гене Текнолоджиз, Инк. | Способ индексации и определения относительной концентрации экспрессирующихся информационных рнк |
-
1998
- 1998-06-30 US US09/108,099 patent/US6096503A/en not_active Expired - Fee Related
-
1999
- 1999-06-30 MX MXPA00012757A patent/MXPA00012757A/es not_active Application Discontinuation
- 1999-06-30 JP JP2000556998A patent/JP2002519011A/ja active Pending
- 1999-06-30 AU AU48497/99A patent/AU784177B2/en not_active Ceased
- 1999-06-30 EP EP99932118A patent/EP1092044A4/en not_active Withdrawn
- 1999-06-30 CA CA002332339A patent/CA2332339A1/en not_active Abandoned
- 1999-06-30 WO PCT/US1999/014852 patent/WO2000000645A1/en not_active Application Discontinuation
-
2000
- 2000-08-01 US US09/630,202 patent/US6633818B1/en not_active Expired - Fee Related
- 2000-12-29 NO NO20006702A patent/NO20006702L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002519011A (ja) | 2002-07-02 |
| EP1092044A4 (en) | 2002-10-30 |
| WO2000000645A1 (en) | 2000-01-06 |
| US6633818B1 (en) | 2003-10-14 |
| AU4849799A (en) | 2000-01-17 |
| EP1092044A1 (en) | 2001-04-18 |
| NO20006702L (no) | 2001-02-27 |
| AU784177B2 (en) | 2006-02-16 |
| NO20006702D0 (no) | 2000-12-29 |
| CA2332339A1 (en) | 2000-01-06 |
| US6096503A (en) | 2000-08-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal |