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MX342195B - Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico. - Google Patents

Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico.

Info

Publication number
MX342195B
MX342195B MX2014003075A MX2014003075A MX342195B MX 342195 B MX342195 B MX 342195B MX 2014003075 A MX2014003075 A MX 2014003075A MX 2014003075 A MX2014003075 A MX 2014003075A MX 342195 B MX342195 B MX 342195B
Authority
MX
Mexico
Prior art keywords
methoxy
methods
unblocked
fast
nucleic acid
Prior art date
Application number
MX2014003075A
Other languages
English (en)
Other versions
MX2014003075A (es
Inventor
Philip Stupi Brian
Li Hong
Wu Weidong
N Hersh Megan
Hertzog David
E Morris Sidney
L Metzker Michael
Original Assignee
Lasergen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lasergen Inc filed Critical Lasergen Inc
Publication of MX2014003075A publication Critical patent/MX2014003075A/es
Publication of MX342195B publication Critical patent/MX342195B/es

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
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Abstract

La presente invención se refiere generalmente a nucleótidos y nucleósidos 3'- OH marcados y no marcados con grupos de terminación que se pueden fotodescomponerse basados en nitrobencilo substituidos con 5-metoxi para usarse en métodos y sistemas relacionados con secuenciación y análisis de ADN y ARN. Estos compuestos pueden ser utilizados como terminadores reversibles ya que exhiben una rápida cinética de incorporación de nucleótido, terminación de base simple, alta selectividad de nucleótido y rápida descomposición de grupo de terminación que da como resultado un nucleótido de origen natural.
MX2014003075A 2011-09-13 2012-09-13 Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico. MX342195B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161534347P 2011-09-13 2011-09-13
US201161627211P 2011-10-07 2011-10-07
PCT/US2012/055231 WO2013040257A1 (en) 2011-09-13 2012-09-13 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing

Publications (2)

Publication Number Publication Date
MX2014003075A MX2014003075A (es) 2014-11-25
MX342195B true MX342195B (es) 2016-09-20

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MX2014003075A MX342195B (es) 2011-09-13 2012-09-13 Nucleótidos de terminación de rápida fotodescomposición 5-metoxi, 3' -oh no bloqueados y métodos para secuenciación de ácido nucleico.

Country Status (10)

Country Link
US (5) US8889860B2 (es)
EP (2) EP2755984B1 (es)
JP (1) JP6196972B2 (es)
KR (1) KR102048274B1 (es)
CN (1) CN104024269B (es)
AU (1) AU2012308518B2 (es)
CA (1) CA2848049C (es)
IL (1) IL231460B (es)
MX (1) MX342195B (es)
WO (1) WO2013040257A1 (es)

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