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MX2021016074A - Nanoparticula con un solo sitio para la union de polinucleotido de plantilla. - Google Patents

Nanoparticula con un solo sitio para la union de polinucleotido de plantilla.

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Publication number
MX2021016074A
MX2021016074A MX2021016074A MX2021016074A MX2021016074A MX 2021016074 A MX2021016074 A MX 2021016074A MX 2021016074 A MX2021016074 A MX 2021016074A MX 2021016074 A MX2021016074 A MX 2021016074A MX 2021016074 A MX2021016074 A MX 2021016074A
Authority
MX
Mexico
Prior art keywords
supercontig
binding
accessory
template
nanoparticle
Prior art date
Application number
MX2021016074A
Other languages
English (en)
Inventor
Lorenzo Berti
Andrew A Brown
Wayne N George
Jonathan Mark Boutell
Ludovic Vincent
M Shane Bowen
Yin Nah Teo
Xiangyuan Yang
Lafranconi Pietro Gatti
Maria Rogert Bacigalupo
Eric Brustad
Mathieu Lessard-Viger
Saurabh Nirantar
Seth Mcdonald
Sergio Peisajovich
Michael Howard
Alex Nemiroski
Jeffrey Brodin
Fei Shen
Lena Storms
Olivia Benice
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2021016074A publication Critical patent/MX2021016074A/es

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/02—Making microcapsules or microballoons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B01J2219/00718—Type of compounds synthesised
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nanotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Composite Materials (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • General Physics & Mathematics (AREA)
  • Materials Engineering (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)

Abstract

Se proporciona una nanopartícula que incluye un supercóntigo, un sitio de plantilla único para unir un polinucleótido de plantilla al supercóntigo, y una pluralidad de sitios accesorios para unir oligonucleótidos accesorios al supercóntigo, en donde el supercóntigo se selecciona de una o más moléculas de ADN de supercóntigo y uno o más polipéptidos de supercóntigo, el sitio de plantilla único para unir un polinucleótido de plantilla al supercóntigo se selecciona de un sitio de unión. de plantilla covalente y un sitio de unión de plantilla no covalente y la pluralidad de sitios accesorios para unir oligonucleótidos accesorios al supercóntigo se seleccionan de sitios de unión de oligonucleótidos accesorios covalentes y sitios de unión de oligonucleótidos accesorios no covalentes. También se proporcionan métodos para usar la nanopartícula, en particular en secuenciación por síntesis.
MX2021016074A 2019-12-23 2020-12-22 Nanoparticula con un solo sitio para la union de polinucleotido de plantilla. MX2021016074A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962952799P 2019-12-23 2019-12-23
US201962952866P 2019-12-23 2019-12-23
PCT/US2020/066547 WO2021133768A1 (en) 2019-12-23 2020-12-22 Nanoparticle with single site for template polynucleotide attachment

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MX2021015230A MX2021015230A (es) 2019-12-23 2020-12-22 Nanoparticula con un solo sitio para la union de polinucleotido de plantilla.

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US (2) US20210187470A1 (es)
EP (2) EP4081662A1 (es)
JP (2) JP2023508800A (es)
KR (2) KR20220119309A (es)
CN (2) CN114207148B (es)
AU (2) AU2020412619A1 (es)
BR (2) BR112021026800A2 (es)
CA (2) CA3144531A1 (es)
IL (3) IL318975A (es)
MX (2) MX2021016074A (es)
PH (2) PH12021553296A1 (es)
TW (2) TW202140798A (es)
WO (2) WO2021133768A1 (es)

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WO2021133770A1 (en) 2021-07-01
US20210187469A1 (en) 2021-06-24
EP4081657A1 (en) 2022-11-02
KR20220119310A (ko) 2022-08-29
IL289230A (en) 2022-02-01
IL289295A (en) 2022-02-01
IL289295B1 (en) 2025-03-01
CA3144531A1 (en) 2021-07-01
CN114245827A (zh) 2022-03-25
MX2021015230A (es) 2022-03-29
AU2020414692A1 (en) 2022-01-06
AU2020412619A1 (en) 2022-01-06
IL289295B2 (en) 2025-07-01
CA3144528A1 (en) 2021-07-01
JP2023508800A (ja) 2023-03-06
IL318975A (en) 2025-04-01
TW202140798A (zh) 2021-11-01
CN114207148B (zh) 2025-04-22
BR112021026800A2 (pt) 2022-07-26
KR20220119309A (ko) 2022-08-29
US20210187470A1 (en) 2021-06-24
PH12021553295A1 (en) 2022-08-01
WO2021133768A1 (en) 2021-07-01
EP4081662A1 (en) 2022-11-02
PH12021553296A1 (en) 2022-08-01
CN114207148A (zh) 2022-03-18
JP2023508799A (ja) 2023-03-06
BR112021026769A2 (pt) 2022-07-26
TW202138565A (zh) 2021-10-16

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