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MX2021012671A - Métodos y materiales para la producción de proteínas. - Google Patents

Métodos y materiales para la producción de proteínas.

Info

Publication number
MX2021012671A
MX2021012671A MX2021012671A MX2021012671A MX2021012671A MX 2021012671 A MX2021012671 A MX 2021012671A MX 2021012671 A MX2021012671 A MX 2021012671A MX 2021012671 A MX2021012671 A MX 2021012671A MX 2021012671 A MX2021012671 A MX 2021012671A
Authority
MX
Mexico
Prior art keywords
materials
production
methods
proteins
nucleotide positions
Prior art date
Application number
MX2021012671A
Other languages
English (en)
Inventor
Smita Shankar
Martin Andrew Hoyt
Xiao Guo
Original Assignee
Impossible Foods Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Impossible Foods Inc filed Critical Impossible Foods Inc
Publication of MX2021012671A publication Critical patent/MX2021012671A/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795Porphyrin- or corrin-ring-containing peptides
    • C07K14/805Haemoglobins; Myoglobins
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1086Preparation or screening of expression libraries, e.g. reporter assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03013Alcohol oxidase (1.1.3.13)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Este documento se refiere a los materiales y métodos para la producción de proteínas. En un aspecto, este documento proporciona un constructo de ácidos nucleicos incluyendo un primer elemento promotor de alcohol oxidasa, en donde el primer elemento promotor de alcohol oxidasa incluye una mutación en una o más posiciones de nucleótidos correspondientes a cualquiera de las posiciones de nucleótidos 668-734 con respecto a la SEO ID NO: 28.
MX2021012671A 2019-04-17 2020-04-17 Métodos y materiales para la producción de proteínas. MX2021012671A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962835338P 2019-04-17 2019-04-17
PCT/US2020/028858 WO2020215017A1 (en) 2019-04-17 2020-04-17 Materials and methods for protein production

Publications (1)

Publication Number Publication Date
MX2021012671A true MX2021012671A (es) 2022-01-24

Family

ID=70614652

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021012671A MX2021012671A (es) 2019-04-17 2020-04-17 Métodos y materiales para la producción de proteínas.

Country Status (13)

Country Link
US (3) US11427932B2 (es)
EP (1) EP3956454A1 (es)
JP (1) JP2022529021A (es)
KR (1) KR20210153106A (es)
CN (1) CN114555812A (es)
AU (1) AU2020257270A1 (es)
BR (1) BR112021020727A2 (es)
CA (1) CA3137100A1 (es)
IL (1) IL287318A (es)
MX (1) MX2021012671A (es)
PH (1) PH12021552635A1 (es)
SG (1) SG11202111468SA (es)
WO (1) WO2020215017A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3294762T3 (da) 2015-05-11 2022-03-14 Impossible Foods Inc Ekspressionskonstruktioner og fremgangsmåder til genmanipulering af methylotrof gær
EP3956454A1 (en) 2019-04-17 2022-02-23 Impossible Foods Inc. Materials and methods for protein production
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
AU2021353004A1 (en) 2020-09-30 2023-04-13 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
WO2022108839A1 (en) * 2020-11-17 2022-05-27 Ginkgo Bioworks, Inc. Synthetic methanol inducible promoters and uses thereof
AU2022369299A1 (en) 2021-10-19 2024-03-14 Eat Scifi Inc. Plant base/animal cell hybrid meat substitute
JP2024546883A (ja) * 2021-12-16 2024-12-26 インポッシブル フーズ インコーポレイテッド 組換えタンパク質の発現を増加させる遺伝因子

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4158065A (en) 1977-12-16 1979-06-12 Sugiyo Co., Ltd. Crab meat substitute and method of preparing same
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5663143A (en) 1988-09-02 1997-09-02 Dyax Corp. Engineered human-derived kunitz domains that inhibit human neutrophil elastase
US5824511A (en) 1995-08-01 1998-10-20 University Technology Corporation Method for enhancing the production of hemoproteins
WO1997028273A1 (en) 1996-02-01 1997-08-07 North American Vaccine, Inc. Expression of group b neisseria meningitidis outer membrane (mb3) protein from yeast and vaccines
DE69728878T2 (de) 1996-06-10 2005-04-07 Novozymes Biotech, Inc., Davis Verfahren zur vermehrung der hemoproteinherstellung in filamentösen fungi
JP3794760B2 (ja) 1996-07-30 2006-07-12 三井化学株式会社 フィターゼ生産酵母
US7803765B2 (en) 1999-05-05 2010-09-28 Phylogica Limited Methods of constructing biodiverse gene fragment libraries and biological modulators isolated therefrom
US7498304B2 (en) * 2000-06-16 2009-03-03 Curis, Inc. Angiogenesis-modulating compositions and uses
WO2004099405A1 (en) 2003-04-11 2004-11-18 Ventria Bioscience Human blood proteins expressed in monocot seeds
CN1308452C (zh) * 2004-09-14 2007-04-04 上海永业农科生物工程有限公司 一种含有改组乙醇氧化酶启动子的毕赤酵母植酸酶表达系统
AT501955B1 (de) 2005-02-23 2007-08-15 Univ Graz Tech Mutierte aox1-promotoren
EP1934329B1 (en) 2005-08-24 2016-11-02 The Scripps Research Institute Translation enhancer-element dependent vector systems
JP2007228956A (ja) 2006-02-28 2007-09-13 Suntory Ltd 醸造用酵母由来の有用タンパク質同定方法
JP2008017733A (ja) 2006-07-11 2008-01-31 Tokyo Univ Of Agriculture 異種タンパク質の発現方法及び異種タンパク質の発現方法により得られたタンパク質
JP2008017774A (ja) 2006-07-13 2008-01-31 Nipro Corp ピキア酵母を用いた組換えヒトヘモグロビンの製造
BRPI0717997A8 (pt) 2006-11-01 2019-01-29 Sigma Alimentos Sa De Cv processo para preparar produtos alimentícios substituídos de carne, pasta para preparar produtos alimentícios substituídos de carne, processo para preparar uma pasta, e, produto alimentício substituto de carne cozido
WO2008090211A1 (en) 2007-01-26 2008-07-31 Novozymes A/S Method for methanol independent induction from methanol inducible promoters in pichia
EP2058398A1 (en) 2007-11-09 2009-05-13 Nipro Corporation Production of recombinant human hemoglobin using pichia yeast
EP2300615A2 (en) 2008-07-11 2011-03-30 Novozymes A/S Pichia pastoris das promoter variants
CN102482638A (zh) 2009-07-02 2012-05-30 微德赞股份有限公司 制备己二酸的生物学方法
WO2011006136A2 (en) 2009-07-09 2011-01-13 Verdezyne, Inc. Engineered microorganisms with enhanced fermentation activity
CA2779163A1 (en) 2009-12-30 2011-07-07 Iogen Energy Corporation Modified yeast strains exhibiting enhanced fermentation of lignocellulosic hydrolysates
CN102190731B (zh) 2010-03-09 2016-01-27 中国科学院上海生命科学研究院 用人工转录因子诱导产生多能干细胞
US9085766B2 (en) 2010-05-21 2015-07-21 Cornell University Methods of producing recombinant heme-binding proteins and uses thereof
WO2012083424A1 (en) 2010-12-21 2012-06-28 The University Of Western Ontario Novel amino acid linker sequences for ligand immobilization
EP2669375B1 (en) 2011-01-27 2018-10-17 Kaneka Corporation Yeast of the genus pichia modified to express high levels of a mpp1 homolog and process for producing protein
WO2012177499A1 (en) 2011-06-23 2012-12-27 The Trustees Of Princeton University Systems for induction of gene expression and protein depletion in yeast
US20140220217A1 (en) 2011-07-12 2014-08-07 Maraxi, Inc. Method and compositions for consumables
US10039306B2 (en) 2012-03-16 2018-08-07 Impossible Foods Inc. Methods and compositions for consumables
KR102024952B1 (ko) 2011-07-12 2019-09-26 임파서블 푸즈 인크. 소비재를 위한 방법 및 조성물
EP2565262A1 (en) 2011-08-31 2013-03-06 VTU Holding GmbH Protein expression
JP6295512B2 (ja) 2012-03-15 2018-03-20 株式会社豊田中央研究所 酵母における外来遺伝子の発現産物の生産方法、酵母における発現調節剤及びその利用
WO2013158749A2 (en) 2012-04-17 2013-10-24 Gevo, Inc. Engineered microorganisms with improved growth properties
CN102757976B (zh) 2012-07-12 2014-10-08 华东理工大学 消除甲醇诱导型启动子单一甲醇碳源依赖性的方法
WO2014045621A1 (ja) 2012-09-19 2014-03-27 国立大学法人大阪大学 肺炎球菌表面タンパク質aを含む肺炎球菌ワクチン
PT2943078T (pt) 2013-01-11 2021-06-16 Impossible Foods Inc Métodos e composições para consumíveis
AU2014305233B2 (en) 2013-08-05 2017-06-29 Agresearch Limited A method and control system for optimising the quality of meat
AU2015240911B2 (en) 2014-03-31 2018-11-08 Impossible Foods Inc. Ground meat replicas
US20160160299A1 (en) 2014-10-31 2016-06-09 Board Of Regents, The University Of Texas System Short exogenous promoter for high level expression in fungi
DK3294762T3 (da) 2015-05-11 2022-03-14 Impossible Foods Inc Ekspressionskonstruktioner og fremgangsmåder til genmanipulering af methylotrof gær
EP3128006A1 (en) 2015-08-06 2017-02-08 Consejo Superior de Investigaciones Cientificas (CSIC) Tools for multiprotein complex expression in pichia pastoris
CA3077201A1 (en) 2017-09-28 2019-04-04 Precision Fermentation, Inc. Methods, devices, and computer program products for yeast performance monitoring in fermentation systems
RU2658758C1 (ru) 2017-10-02 2018-06-22 ООО "Лаборатория Ангиофарм" Рекомбинантная плазмида для экспрессии в дрожжах Pichia pastoris гена химерного белка ангиогенина человека и штамм дрожжей Pichia pastoris - продуцент рекомбинантного химерного белка ангиогенина человека
US20200325484A1 (en) 2019-04-12 2020-10-15 Delin Liang Enhancing gene expression by linking self-amplifying transcription factor with viral 2A-like peptide
EP3956454A1 (en) 2019-04-17 2022-02-23 Impossible Foods Inc. Materials and methods for protein production
AU2020263580A1 (en) 2019-04-25 2021-11-18 Impossible Foods Inc. Strains and methods for production of heme-containing proteins
US20210062206A1 (en) 2019-07-08 2021-03-04 The Regents Of The University Of California Synthetic transcription factors
JP2024546883A (ja) 2021-12-16 2024-12-26 インポッシブル フーズ インコーポレイテッド 組換えタンパク質の発現を増加させる遺伝因子

Also Published As

Publication number Publication date
US20220389616A1 (en) 2022-12-08
KR20210153106A (ko) 2021-12-16
JP2022529021A (ja) 2022-06-16
PH12021552635A1 (en) 2022-07-11
US20250066951A1 (en) 2025-02-27
IL287318A (en) 2021-12-01
CA3137100A1 (en) 2020-10-22
WO2020215017A1 (en) 2020-10-22
SG11202111468SA (en) 2021-11-29
US20200332267A1 (en) 2020-10-22
EP3956454A1 (en) 2022-02-23
BR112021020727A2 (pt) 2021-12-14
US12116699B2 (en) 2024-10-15
US11427932B2 (en) 2022-08-30
CN114555812A (zh) 2022-05-27
AU2020257270A1 (en) 2021-11-18

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