[go: up one dir, main page]

MX2021002573A - Sistemas y procedimientos para electroforesis capilar de afinidad. - Google Patents

Sistemas y procedimientos para electroforesis capilar de afinidad.

Info

Publication number
MX2021002573A
MX2021002573A MX2021002573A MX2021002573A MX2021002573A MX 2021002573 A MX2021002573 A MX 2021002573A MX 2021002573 A MX2021002573 A MX 2021002573A MX 2021002573 A MX2021002573 A MX 2021002573A MX 2021002573 A MX2021002573 A MX 2021002573A
Authority
MX
Mexico
Prior art keywords
systems
methods
capillary electrophoresis
affinity capillary
subject matter
Prior art date
Application number
MX2021002573A
Other languages
English (en)
Inventor
Kelsey Catherine Dent
David John Fischer
David A Michels
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of MX2021002573A publication Critical patent/MX2021002573A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Electrostatic Separation (AREA)

Abstract

La materia objeto descrita en el presente documento se refiere a composiciones, sistemas y procedimientos de cribado de una o más especies de un polipéptido en una mezcla compleja de polipéptidos, por ejemplo, proteínas de múltiples subunidades. Por ejemplo, la materia objeto se refiere a ligandos usados en conexión con la electroforesis capilar de afinidad, así como también a los procedimientos y sistemas de detección de polipéptidos en una mezcla de multímeros que incluye anticuerpos multiespecíficos, por ejemplo, anticuerpos biespecíficos.
MX2021002573A 2018-09-10 2019-09-10 Sistemas y procedimientos para electroforesis capilar de afinidad. MX2021002573A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862729384P 2018-09-10 2018-09-10
PCT/US2019/050361 WO2020055832A1 (en) 2018-09-10 2019-09-10 Systems and methods for affinity capillary electrophoresis

Publications (1)

Publication Number Publication Date
MX2021002573A true MX2021002573A (es) 2021-05-12

Family

ID=68138779

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021002573A MX2021002573A (es) 2018-09-10 2019-09-10 Sistemas y procedimientos para electroforesis capilar de afinidad.

Country Status (11)

Country Link
EP (1) EP3837282A1 (es)
JP (1) JP7482853B2 (es)
KR (1) KR20210056364A (es)
CN (1) CN112654639B (es)
AU (1) AU2019340647A1 (es)
BR (1) BR112021004436A2 (es)
CA (1) CA3109067A1 (es)
IL (1) IL281186A (es)
MX (1) MX2021002573A (es)
TW (1) TWI850255B (es)
WO (1) WO2020055832A1 (es)

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2828426B2 (ja) * 1995-03-31 1998-11-25 株式会社分子バイオホトニクス研究所 蛍光検出等電点電気泳動用マーカー
US20020062010A1 (en) 1997-05-02 2002-05-23 Genentech, Inc. Method for making multispecific antibodies having heteromultimeric and common components
US20030207346A1 (en) * 1997-05-02 2003-11-06 William R. Arathoon Method for making multispecific antibodies having heteromultimeric and common components
JP4213224B2 (ja) 1997-05-02 2009-01-21 ジェネンテック，インコーポレーテッド ヘテロマルチマー及び共通成分を有する多重特異性抗体の製造方法
US20020002269A1 (en) 1998-09-29 2002-01-03 Jeffrey D. Milbrandt Artemin, a neurotrophic factor
CA2463301A1 (en) * 2004-04-22 2005-10-22 Atanas Iliev Lalev Method for detecting protein interactions - affinity purification with switching the ligand
US20090182127A1 (en) 2006-06-22 2009-07-16 Novo Nordisk A/S Production of Bispecific Antibodies
ES2774337T3 (es) 2008-01-07 2020-07-20 Amgen Inc Método para fabricación de moléculas heterodímeras Fc de anticuerpos utilizando efectos de conducción electrostática
CA2851534C (en) 2011-10-10 2023-02-14 Xencor, Inc. A method for purifying antibodies
US10851178B2 (en) 2011-10-10 2020-12-01 Xencor, Inc. Heterodimeric human IgG1 polypeptides with isoelectric point modifications
AR096601A1 (es) 2013-06-21 2016-01-20 Novartis Ag Anticuerpos del receptor 1 de ldl oxidado similar a lectina y métodos de uso
EP3083689B1 (en) 2013-12-17 2020-05-27 Genentech, Inc. Anti-cd3 antibodies and methods of use
EP3294775B1 (en) * 2015-05-12 2021-07-14 Regeneron Pharmaceuticals, Inc. Multimeric protein purity determination
JP7002446B2 (ja) * 2015-09-21 2022-03-04 アプティーボ リサーチ アンド デベロップメント エルエルシー Ｃｄ３結合ポリペプチド

Also Published As

Publication number Publication date
IL281186A (en) 2021-04-29
AU2019340647A1 (en) 2021-03-11
CN112654639B (zh) 2024-08-30
CN112654639A (zh) 2021-04-13
WO2020055832A1 (en) 2020-03-19
JP2022500627A (ja) 2022-01-04
JP7482853B2 (ja) 2024-05-14
KR20210056364A (ko) 2021-05-18
TW202026314A (zh) 2020-07-16
BR112021004436A2 (pt) 2021-05-25
EP3837282A1 (en) 2021-06-23
CA3109067A1 (en) 2020-03-19
TWI850255B (zh) 2024-08-01

Similar Documents

Publication Publication Date Title
ZA202208710B (en) Anti-vegf protein compositions and methods for producing the same
MX2020001227A (es) Composiciones y metodos para detectar y cuantificar la proteina en la celula hospedera en las lineas de celulas y productos de polipeptidos recombinantes.
MX2023003630A (es) Aglutinantes de albumina de suero mejorados.
MY199781A (en) Anti-cd38 antibodies and methods of use
MX2021009352A (es) Compuestos de fijacion al receptor de il -2.
CL2017001921A1 (es) Proteinas de union a icos
EA201892691A1 (ru) Однодоменный белок, связывающий сывороточный альбумин
EA201991168A1 (ru) Белок, связывающий простатический специфический мембранный антиген
BR112018003127A2 (pt) purificação de fkpa e usos do mesmo para produzir polipeptídeos recombinantes
MX2017000083A (es) Compuestos de platino, composiciones y usos de estos.
EP3543335A3 (en) Soluble intein fusion proteins and methods for purifying biomolecules
MX2022005091A (es) Metodos de preparacion de una muestra enriquecida para la secuenciacion de polipeptidos.
AR104610A1 (es) Determinación de pureza de proteínas multiméricas
WO2016172551A8 (en) METHODS OF IDENTIFYING BACTERIA COMPRISING BINDING POLYPEPTIDES
EA201892325A1 (ru) Композиции и способы детектирования белков клетки-хозяина
SA521420953B1 (ar) بروتين ربط مولد ضد مضاد لـمولد ضد ظهاري ذو 6 أغشية انتقالية من البروستاتا 1
MX2020000288A (es) Cromatografia.
EA202190459A1 (ru) Способ и хроматографическая система для определения количества и чистоты мультимерного белка
MX2021002573A (es) Sistemas y procedimientos para electroforesis capilar de afinidad.
MA40371A (fr) Sélectivité efficace des protéines recombinées
MX2020012016A (es) Sistemas y metodos para cuantificar y modificar la viscosidad de proteinas.
BR112019024742A2 (pt) Proteínas robo2 recombinantes, composições, métodos e usos das mesmas
MX2018016413A (es) Ensayos farmacocineticos mejorados para dominios variables unicos de inmunoglobulina.
MA38497A1 (fr) Polypeptides d'il-22 et protéines de fusion fc il-22 et leurs procédés d'utilisation