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MX2020010422A - Alfa-amilasa maltogenica variante. - Google Patents

Alfa-amilasa maltogenica variante.

Info

Publication number
MX2020010422A
MX2020010422A MX2020010422A MX2020010422A MX2020010422A MX 2020010422 A MX2020010422 A MX 2020010422A MX 2020010422 A MX2020010422 A MX 2020010422A MX 2020010422 A MX2020010422 A MX 2020010422A MX 2020010422 A MX2020010422 A MX 2020010422A
Authority
MX
Mexico
Prior art keywords
amino acid
amylase
variant
acid sequence
maltogenic alpha
Prior art date
Application number
MX2020010422A
Other languages
English (en)
Inventor
Aloysius Wilhelmus Rudolphus Hubertus Teunissen
Jong René Marcel De
Joke Anneleen Putseys
Jeroen Godefrooij
Helma Arina Stolze-Lagerweij
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of MX2020010422A publication Critical patent/MX2020010422A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01133—Glucan 1,4-alpha-maltohydrolase (3.2.1.133), i.e. maltogenic alpha-amylase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Abstract

La presente invención se refiere a un polipéptido variante que tiene actividad alfa-amilasa maltogénica, en donde el polipéptido comprende una secuencia de aminoácidos, que, cuando se alinea con una secuencia de aminoácidos de la SEQ ID NO: 1, comprende una sustitución de aminoácidos F188L/I, S200N y D261G y, opcionalmente, otra sustitución de aminoácidos T288P, en donde las sustituciones de aminoácidos se determinan con referencia a la SEQ ID NO: 1, y en donde el polipéptido tiene una secuencia de aminoácidos que tiene al menos 70 %, 75 %, 80 %, 85 %, 90 %, 95 %, 96 %, 97 %, 98 %, o al menos 99 % de identidad con la secuencia de aminoácidos de la SEQ ID NO: 1. La invención también se refiere a un proceso para la preparación de una masa o un producto panificado en donde se usa el polipéptido variante.
MX2020010422A 2018-04-05 2019-04-04 Alfa-amilasa maltogenica variante. MX2020010422A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18165871 2018-04-05
PCT/EP2019/058523 WO2019193102A1 (en) 2018-04-05 2019-04-04 Variant maltogenic alpha-amylase

Publications (1)

Publication Number Publication Date
MX2020010422A true MX2020010422A (es) 2020-10-28

Family

ID=61906748

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020010422A MX2020010422A (es) 2018-04-05 2019-04-04 Alfa-amilasa maltogenica variante.

Country Status (6)

Country Link
US (1) US11944104B2 (es)
EP (1) EP3772966A1 (es)
CN (1) CN111935981B (es)
MX (1) MX2020010422A (es)
WO (1) WO2019193102A1 (es)
ZA (1) ZA202005561B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111944785B (zh) * 2020-08-27 2022-05-06 江南大学 一种产麦芽糖淀粉酶的重组菌株及其在烘焙食品中的应用
AU2023272468A1 (en) 2022-05-14 2024-11-14 Novonesis Plant Biosolutions A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK474589D0 (da) 1989-09-27 1989-09-27 Novo Nordisk As Fremgangsmaade til fremstilling af bageriprodukter
DK2316929T3 (en) 1998-02-27 2016-07-25 Novozymes As Maltogenic alpha-amylase variants
AU2003303934A1 (en) 2002-05-30 2004-09-09 Dsm Ip Assets B.V. Novel pectinases and uses thereof
EP2151494A3 (en) * 2004-08-02 2011-04-13 Novozymes A/S Maltogenic alpha-amylase variants
CA2610683C (en) 2004-09-24 2015-12-22 Novozymes A/S Method of preparing a dough-based product
JP2008527985A (ja) 2005-01-24 2008-07-31 ディーエスエム アイピー アセッツ ビー．ブイ． 糸状菌細胞において目的の化合物を産生させるための方法
JPWO2007094391A1 (ja) * 2006-02-17 2009-07-09 学校法人関西学院 ドメイン間相互作用を利用した遺伝子発現抑制法
PL2423315T3 (pl) 2006-06-29 2015-06-30 Dsm Ip Assets Bv Sposób osiągania lepszej ekspresji polipeptydu
EP2186880A1 (en) 2008-11-07 2010-05-19 DSM IP Assets B.V. Improved production of riboflavin
EP2486799A1 (en) * 2011-02-14 2012-08-15 DSM IP Assets B.V. Method to produce cake with lipolytic enzyme and alpha-amylase
US9301533B2 (en) * 2013-03-01 2016-04-05 Dsm Ip Assets B.V. Alpha-amylase variants
CN105026557A (zh) * 2013-03-01 2015-11-04 帝斯曼知识产权资产管理有限公司 α-淀粉酶变体
WO2015118126A1 (en) 2014-02-07 2015-08-13 Dsm Ip Assets B.V. Improved bacillus host

Also Published As

Publication number Publication date
EP3772966A1 (en) 2021-02-17
US20210161158A1 (en) 2021-06-03
US11944104B2 (en) 2024-04-02
CN111935981B (zh) 2024-06-14
BR112020020252A2 (pt) 2021-01-19
ZA202005561B (en) 2022-03-30
WO2019193102A1 (en) 2019-10-10
CN111935981A (zh) 2020-11-13

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