MX2013001447A - Metodo de produccion y purificacion de una sialiltransferasa soluble activa. - Google Patents
Metodo de produccion y purificacion de una sialiltransferasa soluble activa.Info
- Publication number
- MX2013001447A MX2013001447A MX2013001447A MX2013001447A MX2013001447A MX 2013001447 A MX2013001447 A MX 2013001447A MX 2013001447 A MX2013001447 A MX 2013001447A MX 2013001447 A MX2013001447 A MX 2013001447A MX 2013001447 A MX2013001447 A MX 2013001447A
- Authority
- MX
- Mexico
- Prior art keywords
- sialyltransferase
- polypeptide
- purifying
- producing
- active soluble
- Prior art date
Links
- 102000003838 Sialyltransferases Human genes 0.000 title abstract 5
- 108090000141 Sialyltransferases Proteins 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 4
- 229920001184 polypeptide Polymers 0.000 abstract 5
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 5
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 5
- 241000699802 Cricetulus griseus Species 0.000 abstract 1
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 abstract 1
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 abstract 1
- OVRNDRQMDRJTHS-CBQIKETKSA-N N-Acetyl-D-Galactosamine Chemical compound CC(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@H](O)[C@@H]1O OVRNDRQMDRJTHS-CBQIKETKSA-N 0.000 abstract 1
- MBLBDJOUHNCFQT-UHFFFAOYSA-N N-acetyl-D-galactosamine Natural products CC(=O)NC(C=O)C(O)C(O)C(O)CO MBLBDJOUHNCFQT-UHFFFAOYSA-N 0.000 abstract 1
- 238000011210 chromatographic step Methods 0.000 abstract 1
- 230000013595 glycosylation Effects 0.000 abstract 1
- 238000006206 glycosylation reaction Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 210000001672 ovary Anatomy 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
- C12Y204/99003—Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase (2.4.99.3)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente invención se relaciona con un método para la producción y purificación de un polipéptido de sialiltransferasa, en particular un polipéptido de N-Acetilgalactosamina (Gal NAc)-a-2, 6-sialiltransferasa I (ST6GalNAcI). El método comprende los pasos de producir el polipéptido de sialiltransferasa en una célula de Ovario de Hámster Chino (CHO) y purificar el polipéptido con una combinación de pasos cromatográficos. El método da por resultado en alto rendimiento del polipéptido de sialiltransferasa que es bastante pura y activa. La sialiltransferasa obtenida, en especial ST6GalNAcI, se puede emplear para la glucosilación de proteínas terapéuticas tales como G-CSF.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20100171626 EP2415779B1 (en) | 2010-08-02 | 2010-08-02 | Method of producing and purifying an active soluble sialyltransferase |
| PCT/EP2011/063291 WO2012016984A1 (en) | 2010-08-02 | 2011-08-02 | Method of producing and purifying an active soluble sialyltransferase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2013001447A true MX2013001447A (es) | 2013-11-20 |
Family
ID=42736163
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013001447A MX2013001447A (es) | 2010-08-02 | 2011-08-02 | Metodo de produccion y purificacion de una sialiltransferasa soluble activa. |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US9222081B2 (es) |
| EP (2) | EP2415779B1 (es) |
| JP (1) | JP5984267B2 (es) |
| CN (1) | CN103154017B (es) |
| CA (1) | CA2807397C (es) |
| ES (1) | ES2534936T3 (es) |
| MX (1) | MX2013001447A (es) |
| PT (1) | PT2415779E (es) |
| WO (1) | WO2012016984A1 (es) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013075740A1 (en) * | 2011-11-23 | 2013-05-30 | Sanofi | Antibody purification method |
| CN105026416A (zh) * | 2013-02-06 | 2015-11-04 | 新加坡科技研究局 | 用于降低蛋白质制剂中的聚集物含量的混合的多官能性金属亲和表面 |
| RU2662668C2 (ru) | 2013-05-06 | 2018-07-26 | Санофи | Способ непрерывной многостадийной очистки антител |
| HK1243329A1 (zh) * | 2015-03-31 | 2018-07-13 | Yeda Research And Development Co., Ltd. | 穀氨酸草酰乙酸轉氨酶1(got1)、其製劑、產生方法及用途 |
| KR101667677B1 (ko) * | 2015-05-29 | 2016-10-19 | 한국생명공학연구원 | 재조합 ST6Gal1 단백질을 활성형으로 제조하는 방법 |
| KR102390246B1 (ko) | 2016-12-21 | 2022-04-22 | 에프. 호프만-라 로슈 아게 | 항체의 시험관내 글리코조작에 있어서의 효소의 재사용 |
| WO2018114879A1 (en) | 2016-12-21 | 2018-06-28 | F. Hoffmann-La Roche Ag | Method for in vitro glycoengineering of antibodies |
| ES2930534T3 (es) | 2017-09-04 | 2022-12-16 | 89Bio Ltd | Conjugados de péptidos de FGF-21 mutantes y usos de los mismos |
| WO2019063849A1 (en) * | 2017-12-20 | 2019-04-04 | Dsm Ip Assets B.V. | PURIFICATION OF A POLYPEPTIDE OF INTEREST |
| KR102362777B1 (ko) * | 2018-03-27 | 2022-02-15 | 주식회사 녹십자 | 친화성 크로마토그래피 공정을 포함하는 백일해균 유래 단백질 수득 방법 |
| KR102140531B1 (ko) * | 2018-08-07 | 2020-08-04 | (주)휴온스 | Gly-Tβ4의 제조방법 |
| EP3613486B1 (de) * | 2018-08-24 | 2020-10-07 | UGA Biopharma GmbH | Verfahren und anlage zur reinigung von epo und/oder einem epo-derivat |
| CN113416243B (zh) * | 2021-08-23 | 2021-12-10 | 上海盛迪医药有限公司 | 一种纯化抗体的方法 |
| CN119912505A (zh) * | 2025-04-02 | 2025-05-02 | 浙江熙正霖生物科技有限公司 | 一种从发酵液中提纯唾液酸的方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5032519A (en) | 1989-10-24 | 1991-07-16 | The Regents Of The Univ. Of California | Method for producing secretable glycosyltransferases and other Golgi processing enzymes |
| WO1995018217A1 (fr) | 1993-12-24 | 1995-07-06 | The Institute Of Physical And Chemical Research | Nouvelle synthetase a chaine de sucres, et procede pour produire cette derniere |
| US6280989B1 (en) * | 1999-06-17 | 2001-08-28 | Dmitri Kapitonov | Sialyltransferases |
| MXPA04003333A (es) | 2001-10-10 | 2006-02-22 | Neose Technologies Inc | Remodelado y glicoconjugacion de peptidos. |
| JP4657219B2 (ja) * | 2003-12-03 | 2011-03-23 | バイオジェネリックス アーゲー | ＧｌｙｃｏＰＥＧ化された顆粒球コロニー刺激因子 |
| JP2008501344A (ja) * | 2004-06-03 | 2008-01-24 | ネオス テクノロジーズ インコーポレイティッド | 切り詰め型ＳＴ６ＧａｌＮＡｃＩポリペプチドおよび核酸 |
| KR20080021590A (ko) | 2005-03-24 | 2008-03-07 | 네오스 테크놀로지스, 인크. | 가용성이고 활성인 진핵생물 글리코실트랜스퍼라제의원핵생물 유기체 내에서의 발현 |
| US20060246544A1 (en) | 2005-03-30 | 2006-11-02 | Neose Technologies,Inc. | Manufacturing process for the production of peptides grown in insect cell lines |
| US20090047710A1 (en) | 2005-11-09 | 2009-02-19 | Neose Technologies, Inc. | ST3Gal-1/ST6GalNAc-1 Chimeras |
| US8969532B2 (en) * | 2006-10-03 | 2015-03-03 | Novo Nordisk A/S | Methods for the purification of polypeptide conjugates comprising polyalkylene oxide using hydrophobic interaction chromatography |
| WO2008073620A2 (en) | 2006-11-02 | 2008-06-19 | Neose Technologies, Inc. | Manufacturing process for the production of polypeptides expressed in insect cell-lines |
-
2010
- 2010-08-02 PT PT101716264T patent/PT2415779E/pt unknown
- 2010-08-02 ES ES10171626.4T patent/ES2534936T3/es active Active
- 2010-08-02 EP EP20100171626 patent/EP2415779B1/en active Active
-
2011
- 2011-08-02 CN CN201180047627.7A patent/CN103154017B/zh not_active Expired - Fee Related
- 2011-08-02 JP JP2013522234A patent/JP5984267B2/ja active Active
- 2011-08-02 EP EP11738247.3A patent/EP2601207B1/en active Active
- 2011-08-02 CA CA2807397A patent/CA2807397C/en active Active
- 2011-08-02 WO PCT/EP2011/063291 patent/WO2012016984A1/en active Application Filing
- 2011-08-02 MX MX2013001447A patent/MX2013001447A/es unknown
- 2011-08-02 US US13/813,804 patent/US9222081B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| PT2415779E (pt) | 2015-05-13 |
| JP2013535207A (ja) | 2013-09-12 |
| EP2415779B1 (en) | 2015-01-21 |
| WO2012016984A1 (en) | 2012-02-09 |
| EP2415779A1 (en) | 2012-02-08 |
| CA2807397A1 (en) | 2012-02-09 |
| CN103154017A (zh) | 2013-06-12 |
| US9222081B2 (en) | 2015-12-29 |
| JP5984267B2 (ja) | 2016-09-06 |
| ES2534936T3 (es) | 2015-04-30 |
| EP2601207B1 (en) | 2016-12-28 |
| CA2807397C (en) | 2019-03-19 |
| CN103154017B (zh) | 2016-02-03 |
| US20140057316A1 (en) | 2014-02-27 |
| EP2601207A1 (en) | 2013-06-12 |
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