[go: up one dir, main page]

MX2009002741A - Lineas de celulas mdck que soportan el crecimiento viral para altos titulos y proceso de biorreactor que utiliza las mismas. - Google Patents

Lineas de celulas mdck que soportan el crecimiento viral para altos titulos y proceso de biorreactor que utiliza las mismas.

Info

Publication number
MX2009002741A
MX2009002741A MX2009002741A MX2009002741A MX2009002741A MX 2009002741 A MX2009002741 A MX 2009002741A MX 2009002741 A MX2009002741 A MX 2009002741A MX 2009002741 A MX2009002741 A MX 2009002741A MX 2009002741 A MX2009002741 A MX 2009002741A
Authority
MX
Mexico
Prior art keywords
cell lines
same
mdck cells
high titers
mdck cell
Prior art date
Application number
MX2009002741A
Other languages
English (en)
Inventor
Mark Thompson
Jonathan Liu
Richard Schwartz
Luis Maranga
Simon Hsu
Mridul Ghosh
Ajit Subramanian
Original Assignee
Medimmune Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medimmune Llc filed Critical Medimmune Llc
Publication of MX2009002741A publication Critical patent/MX2009002741A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material
    • C12N2760/16152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16251—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16251—Methods of production or purification of viral material
    • C12N2760/16252—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La presente invención se refiere a las nuevas células MDCK que pueden ser para desarrollar virus, por ejemplo, virus de la influenza, en cultivo celular a un título más alto que lo que era previamente posible. Las células MDCK pueden ser adaptadas al medio de cultivo libre de suero. La presente invención se refiere además a las composiciones de cultivo celular que comprenden las células MDCK y los métodos de cultivo para desarrollar las células MDCK. La presente invención se refiere además a los métodos para producir los virus de la influenza en cultivo celular, utilizando las células MDCK de la invención.
MX2009002741A 2006-09-15 2007-09-14 Lineas de celulas mdck que soportan el crecimiento viral para altos titulos y proceso de biorreactor que utiliza las mismas. MX2009002741A (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US84512106P 2006-09-15 2006-09-15
US87172106P 2006-12-22 2006-12-22
US91700807P 2007-05-09 2007-05-09
US95181307P 2007-07-25 2007-07-25
PCT/US2007/078527 WO2008105931A2 (en) 2006-09-15 2007-09-14 Mdck cell lines supporting viral growth to high titers and bioreactor process using the same

Publications (1)

Publication Number Publication Date
MX2009002741A true MX2009002741A (es) 2009-05-11

Family

ID=39721761

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009002741A MX2009002741A (es) 2006-09-15 2007-09-14 Lineas de celulas mdck que soportan el crecimiento viral para altos titulos y proceso de biorreactor que utiliza las mismas.

Country Status (13)

Country Link
US (3) US20080286850A1 (es)
EP (2) EP2069480B1 (es)
JP (2) JP5260525B2 (es)
KR (2) KR101464783B1 (es)
CN (2) CN103361319A (es)
AU (1) AU2007347716B2 (es)
BR (1) BRPI0716724A2 (es)
CA (1) CA2663522C (es)
ES (1) ES2473275T3 (es)
MX (1) MX2009002741A (es)
RU (2) RU2487935C2 (es)
SG (2) SG10201501780QA (es)
WO (1) WO2008105931A2 (es)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005322353B2 (en) 2004-12-23 2011-09-01 Medimmune, Llc Non-tumorigenic MDCK cell line for propagating viruses
EP2842572A1 (en) * 2005-11-01 2015-03-04 Novartis Vaccines and Diagnostics GmbH Cell-derived viral vaccines with low levels of residual cell dna
US9254318B2 (en) 2006-03-31 2016-02-09 Wisconsin Alumni Research Foundation High titer recombinant influenza viruses for vaccines
MX2009002741A (es) 2006-09-15 2009-05-11 Medimmune Llc Lineas de celulas mdck que soportan el crecimiento viral para altos titulos y proceso de biorreactor que utiliza las mismas.
WO2008156778A2 (en) 2007-06-18 2008-12-24 Tokiko Watanabe Influenza m2 protein mutant viruses as live influenza attenuated vaccines
AU2009296628B2 (en) 2008-09-24 2015-01-15 Medimmune, Llc Methods for purification of viruses
AU2009296701B2 (en) * 2008-09-24 2015-02-19 Medimmune, Llc Methods for cultivating cells, propagating and purifying viruses
WO2010052214A2 (en) * 2008-11-05 2010-05-14 Glaxosmithkline Biologicals S.A. Novel method
JP2010143860A (ja) * 2008-12-19 2010-07-01 Chisso Corp タンパク質の安定化剤
EP2493912B1 (en) 2009-10-26 2020-07-29 Wisconsin Alumni Research Foundation High titer recombinant influenza viruses with enhanced replication in vero cells
US10130697B2 (en) 2010-03-23 2018-11-20 Wisconsin Alumni Research Foundation (Warf) Vaccines comprising mutant attenuated influenza viruses
US20110262965A1 (en) 2010-04-23 2011-10-27 Life Technologies Corporation Cell culture medium comprising small peptides
WO2011135393A1 (es) * 2010-04-27 2011-11-03 Universidad Manuela Beltran Vacuna biologica autologa contra el cancer
AR080972A1 (es) * 2010-04-28 2012-05-23 Abbott Biologicals Bv Produccion de componentes virales
RU2565546C2 (ru) * 2010-08-16 2015-10-20 Севек Фармасьютикалз Гмбх Постоянные линии клеток человека для получения вирусов гриппа
CN101979517B (zh) * 2010-10-15 2011-12-28 普莱柯生物工程股份有限公司 一种利用生物反应器大规模生产流感病毒的方法
WO2013106398A1 (en) * 2012-01-09 2013-07-18 Sanofi Pasteur Biologics, Llc Purification of herpes virus
CN102526720B (zh) * 2012-01-11 2013-12-11 中国人民解放军军事医学科学院微生物流行病研究所 一种流感病毒疫苗的制备方法
JP6152535B2 (ja) * 2012-04-16 2017-06-28 公益財団法人ヒューマンサイエンス振興財団 細胞培養組成物及びインフルエンザウイルスの生産方法
WO2013177172A2 (en) 2012-05-21 2013-11-28 Sanofi Pasteur Limited Herpesvirus compositions and related methods
RU2507255C1 (ru) * 2012-07-23 2014-02-20 Государственное научное учреждение Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии Россельхозакадемии ШТАММ ДИПЛОИДНЫХ КЛЕТОК СИНОВИАЛЬНОЙ МЕМБРАНЫ ЯГНЕНКА Ovis aries, ИСПОЛЬЗУЕМЫЙ ДЛЯ ВИРУСОЛОГИЧЕСКИХ ИССЛЕДОВАНИЙ
RU2506310C1 (ru) * 2012-07-23 2014-02-10 Государственное научное учреждение Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии Россельхозакадемии ШТАММ ДИПЛОИДНЫХ КЛЕТОК СИНОВИАЛЬНОЙ МЕМБРАНЫ ПОРОСЕНКА Sus scrofa, ИСПОЛЬЗУЕМЫЙ ДЛЯ ВИРУСОЛОГИЧЕСКИХ ИССЛЕДОВАНИЙ
CN108379571A (zh) 2012-12-28 2018-08-10 勃林格殷格翰动物保健有限公司 支原体疫苗的制备方法
BR112015015199B1 (pt) * 2012-12-28 2022-09-06 Boehringer Ingelheim Vetmedica Gmbh Composição imunogênica compreendendo antígenos de micoplasma e uso
KR101370512B1 (ko) 2013-06-07 2014-03-06 재단법인 목암생명공학연구소 무단백 배지에서 부유 배양되는 mdck 유래 세포주 및 상기 세포주를 이용하여 바이러스를 증식시키는 방법
WO2015009743A1 (en) 2013-07-15 2015-01-22 Wisconsin Alumni Research Foundation High titer recombinant influenza viruses with enhanced replication in mdck or vero cells or eggs
JP2014088409A (ja) * 2013-12-20 2014-05-15 Jnc Corp タンパク質の安定化剤
WO2015196150A2 (en) 2014-06-20 2015-12-23 Wisconsin Alumni Research Foundation (Warf) Mutations that confer genetic stability to additional genes in influenza viruses
CN104212768A (zh) * 2014-09-18 2014-12-17 中国科学院大连化学物理研究所 用于培养微囊化重组中国仓鼠卵巢细胞的无蛋白培养基及其制备方法
DK3224376T4 (da) * 2014-11-28 2023-05-30 Uniqure Ip Bv DNA-forureninger i en sammensætning omfattende en parvoviral virion
RU2573938C1 (ru) * 2014-12-11 2016-01-27 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук Клеточная линия рака почки человека ibgvat r 27
RU2573940C1 (ru) * 2014-12-11 2016-01-27 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук Клеточная линия рака почки человека ibgvat r 6
CN104689311A (zh) * 2014-12-31 2015-06-10 瑞普（保定）生物药业有限公司 一种生产禽脑脊髓炎病毒灭活疫苗的方法
WO2016148195A1 (ja) * 2015-03-17 2016-09-22 一般財団法人阪大微生物病研究会 インフルエンザウイルス作出用細胞
US10633422B2 (en) 2015-06-01 2020-04-28 Wisconsin Alumni Research Foundation (Warf) Influenza virus replication by inhibiting microRNA lec7C binding to influenza viral cRNA and mRNA
US9890363B2 (en) 2015-07-06 2018-02-13 Wisconsin Alumni Research Foundation (Warf) Influenza virus replication for vaccine development
CN105349499B (zh) * 2015-11-06 2018-12-07 张澍 一种禽流感全病毒颗粒标记疫苗的制备方法及其产品和用途
EP3417056A1 (en) 2016-02-19 2018-12-26 Wisconsin Alumni Research Foundation (WARF) Improved influenza b virus replication for vaccine development
JP6933643B2 (ja) * 2016-03-29 2021-09-08 一般財団法人阪大微生物病研究会 Ｍｄｃｋ細胞の培養方法
CN106119186B (zh) * 2016-06-24 2019-10-08 肇庆大华农生物药品有限公司 一种用于全悬浮培养mdck细胞的无血清培养基及其制备方法
EP3299454A1 (en) * 2016-09-21 2018-03-28 Deutsches Krebsforschungszentrum Optimized method for large scale production of parvovirus h-1 in an essentially serum-free medium
CN106520700B (zh) * 2016-11-10 2019-12-27 中国食品药品检定研究院 稳定表达牛胰蛋白酶原的mdck细胞系及其用途
US11197926B2 (en) 2017-10-25 2021-12-14 Wisconsin Alumni Research Foundation (Warf) Recombinant influenza viruses with stabilized HA for replication in eggs
EP3840780A1 (en) 2018-08-20 2021-06-30 Wisconsin Alumni Research Foundation Vectors for eliciting immune responses to non-dominant epitopes in the hemagglutinin (ha) protein
WO2020106975A1 (en) * 2018-11-21 2020-05-28 Western Oncolytics Ltd. Manufacture of virus
US11241492B2 (en) 2019-01-23 2022-02-08 Wisconsin Alumni Research Foundation (Warf) Mutations that confer genetic stability to genes in influenza viruses
WO2020163804A1 (en) 2019-02-08 2020-08-13 Wisconsin Alumni Research Foundation (Warf) Humanized cell line
CN114929269A (zh) 2019-05-01 2022-08-19 威斯康星校友研究基金会(Warf) 用于疫苗开发的改进的流感病毒复制
WO2021041624A2 (en) 2019-08-27 2021-03-04 Yoshihiro Kawaoka Recombinant influenza viruses with stabilized ha for replication in eggs
WO2021146591A2 (en) * 2020-01-17 2021-07-22 Asklepios Biopharmaceutical, Inc. Recombinant aav production
JP2023524015A (ja) 2020-04-29 2023-06-08 エスケー バイオサイエンス カンパニー リミテッド 使い捨て培養工程システムを用いたインフルエンザウイルス生産方法及びインフルエンザウイルス抗原精製条件の迅速確認試験
KR102444684B1 (ko) * 2020-04-29 2022-09-16 에스케이바이오사이언스(주) 일회용 배양 공정 시스템을 이용한 인플루엔자 바이러스 생산 방법
WO2022066934A2 (en) * 2020-09-23 2022-03-31 Codiak Biosciences, Inc. Process for preparing extracellular vesicles
CN116376810A (zh) * 2023-04-26 2023-07-04 上海奥浦迈生物科技股份有限公司 一种mdck细胞无血清贴壁培养驯化的方法

Family Cites Families (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO162160C (no) 1987-01-09 1989-11-15 Medi Cult As Serumfritt vekstmedium, samt anvendelse derav.
US5118513A (en) 1987-07-02 1992-06-02 The Procter & Gamble Company Method for enhancing bioavailability of iron-calcium mineral supplements
US5854037A (en) 1989-08-28 1998-12-29 The Mount Sinai School Of Medicine Of The City University Of New York Recombinant negative strand RNA virus expression systems and vaccines
US5166057A (en) 1989-08-28 1992-11-24 The Mount Sinai School Of Medicine Of The City University Of New York Recombinant negative strand rna virus expression-systems
US5840520A (en) 1989-08-28 1998-11-24 Aviron Recombinant negative strand RNA virus expression systems
US20060019350A1 (en) 1989-08-28 2006-01-26 Peter Palese Recombinant negative strand RNA virus expression system and vacccines
US5786199A (en) 1989-08-28 1998-07-28 The Mount Sinai School Of Medicine Of The City University Of New York Recombinant negative strand RNA virus expression systems and vaccines
US6001634A (en) 1989-08-28 1999-12-14 Palese; Peter Recombinant negative strand RNA viruses
SU1698288A1 (ru) 1990-02-01 1991-12-15 Всесоюзный научно-исследовательский институт гриппа Штамм VIRUS INFLUeNZa А/Ленинград/325/88 дл приготовлени гриппозного диагностикума
US6887699B1 (en) 1990-05-22 2005-05-03 Medimmune Vaccines, Inc. Recombinant negative strand RNA virus expression systems and vaccines
US6245549B1 (en) 1990-06-28 2001-06-12 Connaught Laboratories Limited Production of virus and purification of viral envelope proteins for vaccine use
GB9215834D0 (en) 1992-07-24 1992-09-09 Celltech Ltd Cell culture
US5824536A (en) 1994-08-23 1998-10-20 St. Jude Children's Research Hospital Influenza virus replicated in mammalian cell culture and vaccine production
US5690937A (en) 1995-06-05 1997-11-25 Aviron Temperature sensitive clustered changed-to-alanine mutants of influenza virus PB2 gene
DE19612967A1 (de) 1996-04-01 1997-10-02 Behringwerke Ag Verfahren zur Vermehrung von Influenzaviren in Zellkultur, sowie die durch das Verfahren erhältlichen Influenzaviren
DE19612966B4 (de) 1996-04-01 2009-12-10 Novartis Vaccines And Diagnostics Gmbh & Co. Kg MDCK-Zellen und Verfahren zur Vermehrung von Influenzaviren
JP2000517188A (ja) 1996-08-30 2000-12-26 ライフ テクノロジーズ，インコーポレイテッド 無血清哺乳動物細胞培養培地およびその使用
US20040171152A1 (en) 1996-10-10 2004-09-02 Invitrogen Corporation Animal cell culture media comprising non-animal or plant-derived nutrients
CA2272820C (en) * 1996-11-20 2012-09-11 Introgen Therapeutics, Inc. An improved method for the production and purification of adenoviral vectors
TW570803B (en) 1997-04-09 2004-01-11 Duphar Int Res Influenza vaccine
US6884414B1 (en) 1997-04-30 2005-04-26 Mount Sinai School Of Medicine Of New York University Recombinant influenza viruses expressing tumor-associated antigens as antitumor agents
US6544785B1 (en) 1998-09-14 2003-04-08 Mount Sinai School Of Medicine Of New York University Helper-free rescue of recombinant negative strand RNA viruses
US8715940B2 (en) 1999-04-06 2014-05-06 Wisconsin Alumni Research Foundation Method of making recombinant influenza virus
JP4972261B2 (ja) 1999-08-27 2012-07-11 ライフ テクノロジーズ コーポレーション 金属結合化合物および細胞培養培地組成物中でのそれらの使用
US6825036B2 (en) 2000-03-03 2004-11-30 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Cell usable in serum-free culture and suspension culture and process for producing virus for vaccine by using the cell
ATE420189T1 (de) 2000-04-28 2009-01-15 St Jude Childrens Res Hospital Dna-transfektionssystem zur erzeugung von infektiösen negativsträngigen rna virus
CN1194005C (zh) * 2000-06-01 2005-03-23 长春长生生物科技股份有限公司 利用Sepharose4FF柱层析技术生产流行性感冒灭活疫苗
AU2001263896A1 (en) 2000-06-23 2002-01-14 Akzo Nobel N.V. Attenuated bovine respiratory syncytial virus
US20040241139A1 (en) 2000-07-20 2004-12-02 Gerd Hobom Recombinant influenza viruses with bicistronic vRNAs coding for two genes in tandem arrangement
US6593123B1 (en) * 2000-08-07 2003-07-15 Avigen, Inc. Large-scale recombinant adeno-associated virus (rAAV) production and purification
DE10144903A1 (de) 2001-09-12 2003-03-27 Chiron Behring Gmbh & Co Vermehrung von Viren in Zellkultur
DE10144906B4 (de) 2001-09-12 2013-11-28 Novartis Vaccines And Diagnostics Gmbh Verfahren zur großtechnischen Herstellung von Impfstoffen
CN100547069C (zh) 2001-12-07 2009-10-07 克鲁塞尔荷兰公司 病毒、病毒分离物和疫苗的生产
US6951752B2 (en) 2001-12-10 2005-10-04 Bexter Healthcare S.A. Method for large scale production of virus antigen
AU2003230559B2 (en) 2002-02-21 2008-11-13 Mount Sinai School Of Medicine Recombinant negative strand virus RNA expression systems and vaccines
AU2003230908A1 (en) 2002-04-11 2003-10-27 Medimmune Vaccines, Inc. Spray freeze dry of compositions for intranasal administration
CN103540568A (zh) 2002-04-26 2014-01-29 米迪缪尼有限公司 制备流感病毒的多质粒系统
US7465456B2 (en) 2002-04-26 2008-12-16 Medimmune, Llc Multi plasmid system for the production of influenza virus
NZ538094A (en) 2002-07-09 2007-01-26 Baxter Int Serum free and animal protein free culture medium for cultivation of cells
EP1585964A4 (en) 2002-08-28 2008-07-16 Introgen Therapeutics Inc CHROMATOGRAPHIC METHODS FOR PURIFYING ADENOVIRUSES
US20060002862A1 (en) 2002-12-17 2006-01-05 Medimmune Vaccines, Inc. High pressure spray-dry of bioactive materials
CN101804202B (zh) 2003-02-25 2014-06-18 米迪缪尼有限公司 制备流感疫苗组合物的方法
US7687240B2 (en) 2003-03-18 2010-03-30 Wyeth Holdings Corporation Process for increasing RSV surface glycoprotein yields using a mutant strain of RSV
US7405068B2 (en) * 2003-05-02 2008-07-29 Tate & Lyle Ingredients Americas, Inc. Pyruvate producing yeast strain
US7723094B2 (en) 2003-05-28 2010-05-25 Wisconsin Alumni Research Foundation Recombinant influenza vectors with a polII promoter and ribozymes for vaccines and gene therapy
WO2004110484A1 (fr) 2003-06-18 2004-12-23 Gosudarstvenny Nauchny Tsentr Virusologii I Biotekhnologii 'vektor' Procede de production d'un vaccin vivant de culture contre le virus de la grippe
AU2003249471A1 (en) 2003-07-31 2005-02-14 Louis Auer Cardiovascular disorder simulation device
WO2005026333A1 (ja) 2003-09-09 2005-03-24 Japan Science And Technology Agency プロテアーゼを用いた接着性細胞の浮遊培養技術、及び該細胞を宿主として用いてウィルスを生産する方法
ATE469972T1 (de) 2003-12-23 2010-06-15 Medimmune Llc Multiplasmid-system zur erzeugung des grippevirus
US7553665B2 (en) 2004-03-01 2009-06-30 Ares Trading S.A. Process for the cultivation of mammalian cells producing IL-18BP in serum-free cell culture medium
US8221969B2 (en) 2004-04-19 2012-07-17 Sanyo Chemical Industries, Ltd. Method of producing virus
KR20070015178A (ko) 2004-04-27 2007-02-01 백스터 인터내셔널 인코포레이티드 교반-탱크 반응기 시스템
WO2005113758A1 (en) 2004-05-20 2005-12-01 Id Biomedical Corporation Process for the production of an influenza vaccine
JP4629385B2 (ja) * 2004-08-16 2011-02-09 Ｎｔｎ株式会社 針状ころ軸受
KR101346989B1 (ko) 2004-10-06 2014-02-06 메디뮨 엘엘씨 냉장 온도 안정성 인플루엔자 백신 조성물
CA2593036A1 (en) 2004-12-08 2006-06-15 Medimmune Vaccines, Inc. Methods of producing influenza vaccine compositions
AU2005322353B2 (en) 2004-12-23 2011-09-01 Medimmune, Llc Non-tumorigenic MDCK cell line for propagating viruses
WO2006095431A1 (ja) 2005-03-10 2006-09-14 Kyoritsu Seiyaku Corporation 動物由来の成分なしで培養可能である細胞株及びその作出方法、これを用いたウイルスの生産方法、及びワクチンの生産方法
US7534596B2 (en) 2006-02-10 2009-05-19 Solohill Engineering, Inc. Method and device for producing vaccine
EP2007883B1 (en) 2006-04-20 2011-12-28 Wyeth LLC Purification processes for isolating purified vesicular stomatitis virus from cell culture
US8778353B2 (en) 2006-05-01 2014-07-15 Technovax, Inc. Influenza virus-like particle (VLP) compositions
MX2009002741A (es) 2006-09-15 2009-05-11 Medimmune Llc Lineas de celulas mdck que soportan el crecimiento viral para altos titulos y proceso de biorreactor que utiliza las mismas.
EP1982727A1 (en) 2007-04-17 2008-10-22 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for purification of viral proteins
AU2009296701B2 (en) 2008-09-24 2015-02-19 Medimmune, Llc Methods for cultivating cells, propagating and purifying viruses
AU2009296628B2 (en) 2008-09-24 2015-01-15 Medimmune, Llc Methods for purification of viruses

Also Published As

Publication number Publication date
CA2663522C (en) 2015-06-02
EP2069480A4 (en) 2011-03-30
US8357376B2 (en) 2013-01-22
AU2007347716B2 (en) 2013-06-20
CN103361319A (zh) 2013-10-23
ES2473275T3 (es) 2014-07-04
KR20140075022A (ko) 2014-06-18
EP2069480A2 (en) 2009-06-17
CA2663522A1 (en) 2008-09-04
KR20090057444A (ko) 2009-06-05
JP2010503412A (ja) 2010-02-04
AU2007347716A1 (en) 2008-09-04
US8846032B2 (en) 2014-09-30
CN101627113B (zh) 2013-04-24
RU2009113671A (ru) 2010-10-20
RU2012145173A (ru) 2014-04-27
US20080286850A1 (en) 2008-11-20
SG174813A1 (en) 2011-10-28
US20130052717A1 (en) 2013-02-28
WO2008105931A3 (en) 2009-02-19
EP2069480B1 (en) 2014-03-19
SG10201501780QA (en) 2015-04-29
BRPI0716724A2 (pt) 2013-10-01
EP2615167A1 (en) 2013-07-17
JP5260525B2 (ja) 2013-08-14
CN101627113A (zh) 2010-01-13
WO2008105931A2 (en) 2008-09-04
RU2487935C2 (ru) 2013-07-20
JP2013153762A (ja) 2013-08-15
US20100112669A1 (en) 2010-05-06
KR101464783B1 (ko) 2014-11-26

Similar Documents

Publication Publication Date Title
MX2009002741A (es) Lineas de celulas mdck que soportan el crecimiento viral para altos titulos y proceso de biorreactor que utiliza las mismas.
MX2007005132A (es) Medios libres de proteina animal para cultivo de celulas.
AU2019268194A1 (en) Cell culture system
EP4368701A3 (en) Feeder-free culture system
SG178914A1 (en) Stem cell conditioned medium compositions
CY1120911T1 (el) Παραγομενες απο mdck κυτταρικες σειρες προσαρμοσμενες σε απαλλαγμενη απο ορο καλλιεργεια και καλλιεργεια εναιωρηματος και μεθοδος για παρασκευη ιου εμβολιου χρησιμοποιωντας τα κυτταρα
MY183385A (en) Production of polio virus at high titers for vaccine production
MX2011011491A (es) Manipulacion de la biosintesis de fructosano y aumento de biomasa vegetal.
WO2011075686A3 (en) Methods & compositions comprising heat shock proteins
WO2010096588A3 (en) Cell culture media containing combinations of proteins
EA201270224A1 (ru) Среда для культивирования клеток для экспрессии белков adamts
WO2011159359A3 (en) Serum-free chemically defined cell culture medium
WO2012171026A3 (en) Methods for high yield virus production
MXPA05009096A (es) Metodo de cultivo celular libre de animal.
SG169359A1 (en) A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses
EA201070788A1 (ru) Трансгенные фотосинтетические микроорганизмы и фотобиореактор
WO2012031263A3 (en) Cell culture system for bioreactor scale-up of cells
CN103509749A (zh) 人参形成层干细胞分离及培养方法
CN102283109B (zh) 一种香蕉新品种的培育方法
CN102090342A (zh) 一种紫花野菊高效再生体系建立的方法
MX2013005140A (es) Medios de cultivo de celulas granulados en seco.
WO2012141471A3 (ko) 역분화-증진제를 이용한 역분화 만능 줄기세포의 제조방법
EA201000901A1 (ru) Способ культивирования микроорганизмов, относящихся к порядку thraustochytriales
CN102690841B (zh) 一种获得红豆杉转基因愈伤组织的遗传转化方法
CN105145365A (zh) 一种中华桫椤绿色球状体途径的组培繁殖方法

Legal Events

Date Code Title Description
FG Grant or registration