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MX2008011406A - Amplificacion especifica de secuencias de adn fetal de una fuente mixta, materno fetal. - Google Patents

Amplificacion especifica de secuencias de adn fetal de una fuente mixta, materno fetal.

Info

Publication number
MX2008011406A
MX2008011406A MX2008011406A MX2008011406A MX2008011406A MX 2008011406 A MX2008011406 A MX 2008011406A MX 2008011406 A MX2008011406 A MX 2008011406A MX 2008011406 A MX2008011406 A MX 2008011406A MX 2008011406 A MX2008011406 A MX 2008011406A
Authority
MX
Mexico
Prior art keywords
fetal
dna sequences
mixed
specific amplification
fetal dna
Prior art date
Application number
MX2008011406A
Other languages
English (en)
Inventor
Stephen Brown
Original Assignee
Univ Columbia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Columbia filed Critical Univ Columbia
Publication of MX2008011406A publication Critical patent/MX2008011406A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención brinda un método para selectivamente amplificar secuencias de ADN fetal de una fuente mixta, materno-fetal. Este método utiliza metilación diferencial para permitir para la amplificación selectiva de secuencias específicas trofoblasto/fetales de mezclas de ADN que contengan una alta proporción de ADN no-trofoblasto/fetales. La invención también brinda métodos para el uso de secuencias de ADN fetales para la detección de aneuploidia.
MX2008011406A 2006-03-06 2007-03-06 Amplificacion especifica de secuencias de adn fetal de una fuente mixta, materno fetal. MX2008011406A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US77891106P 2006-03-06 2006-03-06
PCT/US2007/063366 WO2007103910A2 (en) 2006-03-06 2007-03-06 Specific amplification of fetal dna sequences from a mixed, fetal-maternal source

Publications (1)

Publication Number Publication Date
MX2008011406A true MX2008011406A (es) 2008-11-18

Family

ID=38475791

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2008011406A MX2008011406A (es) 2006-03-06 2007-03-06 Amplificacion especifica de secuencias de adn fetal de una fuente mixta, materno fetal.

Country Status (11)

Country Link
US (1) US20090203002A1 (es)
EP (1) EP1994164A4 (es)
JP (1) JP2009529330A (es)
KR (1) KR20080107464A (es)
CN (1) CN101421410A (es)
AU (1) AU2007223102A1 (es)
BR (1) BRPI0709545A2 (es)
CA (1) CA2645045A1 (es)
MX (1) MX2008011406A (es)
WO (1) WO2007103910A2 (es)
ZA (1) ZA200808153B (es)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
EP2423334A3 (en) 2006-02-02 2012-04-18 The Board of Trustees of The Leland Stanford Junior University Non-invasive fetal genetic screening by digital analysis
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
EP2589668A1 (en) 2006-06-14 2013-05-08 Verinata Health, Inc Rare cell analysis using sample splitting and DNA tags
US8748100B2 (en) * 2007-08-30 2014-06-10 The Chinese University Of Hong Kong Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) * 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
WO2010075459A1 (en) 2008-12-22 2010-07-01 Celula, Inc. Methods and genotyping panels for detecting alleles, genomes, and transcriptomes
US20100285537A1 (en) * 2009-04-02 2010-11-11 Fluidigm Corporation Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation
US9447467B2 (en) 2009-04-21 2016-09-20 Genetic Technologies Limited Methods for obtaining fetal genetic material
US8563242B2 (en) * 2009-08-11 2013-10-22 The Chinese University Of Hong Kong Method for detecting chromosomal aneuploidy
EP2488644A4 (en) * 2009-10-14 2013-03-27 Genetic Technologies Ltd Epigenetic dna enrichment
US20120315633A1 (en) 2009-12-23 2012-12-13 Genetic Technologies Limited Methods of enriching and detecting fetal nucleic acids
WO2011082386A1 (en) * 2009-12-31 2011-07-07 The Trustees Of Columbia University In The City Of New York Specific amplification of fetal dna sequences from a mixed, fetal-maternal source
WO2012018391A2 (en) 2010-08-02 2012-02-09 Guided Therapy Systems, Llc Methods and systems for treating plantar fascia
BR112013010585B1 (pt) * 2010-10-29 2020-12-01 Asuragen, Inc. método de caracterização de um locus de fmr1 ou um locus de frm2 em uma amostra de dna e método de análise de um locus fmr1 ou um locus fmr2 de uma amostra de dna humana
GB2488358A (en) * 2011-02-25 2012-08-29 Univ Plymouth Enrichment of foetal DNA in maternal plasma
AU2012340118A1 (en) * 2011-11-17 2014-04-24 Rheonix, Inc. System and methods for selective molecular analysis
KR101256206B1 (ko) * 2012-03-02 2013-04-19 의료법인 제일의료재단 태아의 성별 결정을 위한 분석방법 및 장치
ES2930180T3 (es) 2012-03-02 2022-12-07 Sequenom Inc Métodos para enriquecer ácido nucleico canceroso a partir de una muestra biológica
CA2868836C (en) 2012-03-26 2019-08-06 The Johns Hopkins University Rapid aneuploidy detection
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
EP2872648B1 (en) 2012-07-13 2019-09-04 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
HK1216655A1 (zh) 2013-03-13 2016-11-25 Sequenom, Inc. 用於dna甲基化分析的引物
WO2015138774A1 (en) 2014-03-13 2015-09-17 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CN107002080B (zh) * 2014-12-18 2020-11-06 深圳华大智造科技股份有限公司 一种基于多重pcr的目标区域富集方法和试剂
EP3518974A4 (en) 2016-09-29 2020-05-27 Myriad Women's Health, Inc. NON-INVASIVE PRENATAL SCREENING USING DYNAMIC ITERATIVE DEEP OPTIMIZATION
CN108588064B (zh) * 2018-04-23 2019-07-26 上海桐树生物科技有限公司 构建目的序列dna文库的试剂盒及目的序列dna文库的构建方法
CN111876472B (zh) * 2020-06-17 2023-12-01 江门市灿明生物科技有限公司 多种混合核酸中检测痕量核酸的方法

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5641628A (en) * 1989-11-13 1997-06-24 Children's Medical Center Corporation Non-invasive method for isolation and detection of fetal DNA
US5714325A (en) * 1993-09-24 1998-02-03 New England Medical Center Hospitals Prenatal diagnosis by isolation of fetal granulocytes from maternal blood
US20070269799A9 (en) * 1994-06-22 2007-11-22 Zhang David Y Nucleic acid amplification methods
US20010051341A1 (en) * 1997-03-04 2001-12-13 Isis Innovation Limited Non-invasive prenatal diagnosis
US7083924B2 (en) * 2000-07-10 2006-08-01 Btg International Limited Diagnostic method for the identification of foetal DNA in a material sample
GB0016742D0 (en) * 2000-07-10 2000-08-30 Simeg Limited Diagnostic method
US6664056B2 (en) * 2000-10-17 2003-12-16 The Chinese University Of Hong Kong Non-invasive prenatal monitoring
US20030036100A1 (en) * 2001-04-10 2003-02-20 Imperial College Innovations Ltd. Simultaneous determination of phenotype and genotype
US20030170675A1 (en) * 2001-04-11 2003-09-11 The Gov't Of The U.S Of America As Represented By The Secretary Of The Dept. Of Health & Human Serv. Methods of manipulating nucleic acids
US7348139B1 (en) * 2001-04-13 2008-03-25 The Johns Hopkins University School Of Medicine SOCS-1 gene methylation in cancer
US6927028B2 (en) * 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
EP1468104A4 (en) * 2002-01-18 2006-02-01 Genzyme Corp METHODS FOR DETECTION OF FETAL DNA AND QUANTIFICATION OF ALLELES
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
US7442506B2 (en) * 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US20070178478A1 (en) * 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
EP1583846B1 (en) * 2003-01-17 2011-11-16 The Chinese University Of Hong Kong Circulating mrna as diagnostic markers for pregnancy-related disorders
WO2004081183A2 (en) * 2003-03-07 2004-09-23 Rubicon Genomics, Inc. In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna
EP2354253A3 (en) * 2003-09-05 2011-11-16 Trustees of Boston University Method for non-invasive prenatal diagnosis
EP1689884A4 (en) * 2003-10-08 2007-04-04 Univ Boston PROCESS FOR THE PRENATAL DIAGNOSIS OF CHROMOSOMAL ABNORMALITIES
DE60328193D1 (de) * 2003-10-16 2009-08-13 Sequenom Inc Nicht invasiver Nachweis fötaler genetischer Merkmale
US20070111233A1 (en) * 2003-10-30 2007-05-17 Bianchi Diana W Prenatal diagnosis using cell-free fetal DNA in amniotic fluid
AU2004286845A1 (en) * 2003-10-30 2005-05-19 Tufts-New England Medical Center Prenatal diagnosis using cell-free fetal DNA in amniotic fluid
US20060003342A1 (en) * 2004-01-15 2006-01-05 Bianchi Diana W Fetal RNA in amniotic fluid to determine gene expression in the developing fetus
EP1721014B1 (en) * 2004-02-18 2013-07-17 Trustees Of Boston University Method for detecting and quantifying rare mutations/polymorphisms
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
WO2005108618A2 (en) * 2004-04-30 2005-11-17 Applera Corporation Methods and kits for methylation detection
US7709194B2 (en) * 2004-06-04 2010-05-04 The Chinese University Of Hong Kong Marker for prenatal diagnosis and monitoring
WO2006097049A1 (en) * 2005-03-18 2006-09-21 The Chinese University Of Hong Kong A method for the detection of chromosomal aneuploidies
US20070122823A1 (en) * 2005-09-01 2007-05-31 Bianchi Diana W Amniotic fluid cell-free fetal DNA fragment size pattern for prenatal diagnosis
EP2423334A3 (en) * 2006-02-02 2012-04-18 The Board of Trustees of The Leland Stanford Junior University Non-invasive fetal genetic screening by digital analysis
WO2007112418A2 (en) * 2006-03-28 2007-10-04 Baylor College Of Medicine Screening for down syndrome
WO2007121276A2 (en) * 2006-04-12 2007-10-25 Biocept, Inc. Enrichment of circulating fetal dna
US7901884B2 (en) * 2006-05-03 2011-03-08 The Chinese University Of Hong Kong Markers for prenatal diagnosis and monitoring
US20080176237A1 (en) * 2006-12-07 2008-07-24 Biocept, Inc. Non-invasive prenatal genetic screen

Also Published As

Publication number Publication date
ZA200808153B (en) 2009-06-24
CA2645045A1 (en) 2007-09-13
WO2007103910A2 (en) 2007-09-13
CN101421410A (zh) 2009-04-29
WO2007103910A3 (en) 2007-11-29
EP1994164A4 (en) 2010-07-21
US20090203002A1 (en) 2009-08-13
JP2009529330A (ja) 2009-08-20
AU2007223102A1 (en) 2007-09-13
EP1994164A2 (en) 2008-11-26
BRPI0709545A2 (pt) 2011-07-19
KR20080107464A (ko) 2008-12-10

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