MD830Z - Process for pre-sowing treatment of carrot seeds - Google Patents
Process for pre-sowing treatment of carrot seeds Download PDFInfo
- Publication number
- MD830Z MD830Z MDS20140053A MDS20140053A MD830Z MD 830 Z MD830 Z MD 830Z MD S20140053 A MDS20140053 A MD S20140053A MD S20140053 A MDS20140053 A MD S20140053A MD 830 Z MD830 Z MD 830Z
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- Moldova
- Prior art keywords
- seeds
- carrot
- carrot seeds
- germination
- sowing treatment
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- 244000000626 Daucus carota Species 0.000 title claims abstract description 16
- 235000002767 Daucus carota Nutrition 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 title claims abstract description 12
- 238000009331 sowing Methods 0.000 title claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229930182470 glycoside Natural products 0.000 claims abstract description 8
- 150000002338 glycosides Chemical class 0.000 claims abstract description 8
- 239000007864 aqueous solution Substances 0.000 claims abstract description 6
- 238000002791 soaking Methods 0.000 claims abstract description 6
- 241000208280 Hyoscyamus niger Species 0.000 claims abstract description 5
- 238000000605 extraction Methods 0.000 claims abstract description 5
- 229930186308 hyoscyamoside Natural products 0.000 description 9
- 230000035784 germination Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 240000004160 Capsicum annuum Species 0.000 description 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
- 241000195955 Equisetum hyemale Species 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 229930002534 steroid glycoside Natural products 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
Invenţia se referă la agricultură, şi anume la un procedeu de tratare a seminţelor de morcov înainte de semănat. The invention relates to agriculture, namely to a process for treating carrot seeds before sowing.
În prezent, este cunoscut procedeul de tratare a seminţelor de morcov înainte de semănat care constă în înmuierea acestora în soluţia apoasă a preparatului moldstim de 0,001% timp de 24 ore. Dezavantajul acestui procedeu constă în faptul că preparatul moldstim este obţinut din materie primă costisitoare - seminţe de ardei dulce, eficacitatea metodei de extragere a glicozidei nu a fost suficient verificată [1]. Currently, the method of treating carrot seeds before sowing is known, which consists in soaking them in an aqueous solution of the 0.001% moldstim preparation for 24 hours. The disadvantage of this method is that the moldstim preparation is obtained from expensive raw materials - sweet pepper seeds, the effectiveness of the glycoside extraction method has not been sufficiently verified [1].
Problema pe care o rezolvă invenţia propusă constă în sporirea germinaţiei seminţelor de morcov în câmp deschis, care asigură răsărirea şi creşterea uniformă a plantulelor, precum şi îmbunătăţirea calităţilor comerciale ale rizocarpilor. The problem solved by the proposed invention consists in increasing the germination of carrot seeds in the open field, which ensures the emergence and uniform growth of seedlings, as well as improving the commercial qualities of rhizocarps.
Procedeul de tratare a seminţelor de morcov înainte de semănat, conform invenţiei, include înmuierea acestora timp de 15 min într-o soluţie apoasă de 0,005% de glicozide furostanolice, obţinute din seminţe de Hyoscyamus niger L. prin extragere cu etanol de 70%, ( în continuare - hiosciamozide). The process for treating carrot seeds before sowing, according to the invention, includes soaking them for 15 min in an aqueous solution of 0.005% furostanol glycosides, obtained from Hyoscyamus niger L. seeds by extraction with 70% ethanol, (hereinafter - hyoscyamosides).
Rezultatul constă în sporirea germinaţiei seminţelor de morcov în câmp deschis, care asigură răsărirea şi creşterea uniformă a plantulelor, precum şi îmbunătăţirea calităţilor comerciale ale rizocarpilor. The result is increased germination of carrot seeds in the open field, which ensures uniform emergence and growth of seedlings, as well as improving the commercial qualities of rhizocarps.
Metoda de obţinere a sumei hiosciamozidelor furostanolice include următoarele operaţiuni: seminţele de măselariţă neagră uscate în aer liber şi fărâmiţate au fost supuse extracţiei prin încălzire cu alcool etilic de 70%. Etanolul a fost distilat din extract, iar reziduul apos obţinut a fost supus filtrării gel prin sephadex G-25 şi G-50, utilizând apa distilată în calitate de eluent. Procesul eluării se controlează permanent prin cromatografiere în strat subţire de silicagel. Eluatele, care conţin glicozide din seria furostanolului, au fost unite şi concentrate prin evaporare în vid, după care au fost uscate. Ca rezultat s-a obţinut suma glicozidelor furostanolice (hiosciamozidelor) sub formă de pulbere de culoare brună deschisă [Nedova I. Structura glicozidelor steroidice din seminţele de Hyoscyamus niger L. şi activitatea lor biologică. Autoreferat al tezei de doctor în chimie, Chişinău, 2008]. The method for obtaining the sum of furostanol hyoscyamosides includes the following operations: the seeds of black horsetail dried in the open air and crushed were subjected to extraction by heating with 70% ethyl alcohol. Ethanol was distilled from the extract, and the aqueous residue obtained was subjected to gel filtration through sephadex G-25 and G-50, using distilled water as an eluent. The elution process was permanently controlled by silica gel thin layer chromatography. The eluates, which contain glycosides from the furostanol series, were combined and concentrated by evaporation in vacuo, after which they were dried. As a result, the sum of furostanol glycosides (hyoscyamosides) was obtained in the form of a light brown powder [Nedova I. Structure of steroid glycosides from Hyoscyamus niger L. seeds and their biological activity. Abstract of the doctoral thesis in chemistry, Chisinau, 2008].
Exemplu de realizare a invenţiei Example of embodiment of the invention
Cercetările s-au desfaşurat în condiţii de laborator şi în câmp deschis. În calitate de obiect de studiu a servit soiul de morcov Krasavka. În scopul determinării concentraţiei optimale de hiosciamozide, preventiv a fost efectuată experienţa în laborator. Seminţele de morcov au fost înmuiate timp de 15 min în soluţiile apoase de hiosciamozide în limitele concentraţiilor de 0,0001%……..0,01%. The research was carried out in laboratory conditions and in the open field. The object of study was the carrot variety Krasavka. In order to determine the optimal concentration of hyoscyamosides, a laboratory experiment was carried out as a preventive measure. Carrot seeds were soaked for 15 min in aqueous solutions of hyoscyamosides in the concentration limits of 0.0001%……..0.01%.
Rezultatele obţinute ne permit să concluzionăm că concentraţia optimă a soluţiei de hiosciamozide pentru sporirea germinaţiei seminţelor de morcov este de 0,005% (tabelul 1). The results obtained allow us to conclude that the optimal concentration of hyoscyamoside solution for increasing carrot seed germination is 0.005% (table 1).
Tabelul 1 Table 1
Influenţa glicozidelor asupra germinaţiei seminţelor de morcov The influence of glycosides on carrot seed germination
Varianta Concentraţia Germinaţia totală % % faţă de martor Martor 35,5 Moldstim 0,0001 37,5 5,6 0,001 36,3 2,1 0,005 35,8 0,7 0,01 44,0 23,9 Hiosciamozide 0,0001 42,5 19,7 0,001 35,5 0 0,005 46,0 29,8 0,01 38,5 8,5Variant Concentration Total germination % % compared to control Control 35.5 Moldstim 0.0001 37.5 5.6 0.001 36.3 2.1 0.005 35.8 0.7 0.01 44.0 23.9 Hyosciamoside 0.0001 42.5 19.7 0.001 35.5 0 0.005 46.0 29.8 0.01 38.5 8.5
În scopul determinării germinaţiei seminţelor de morcov în câmp deschis, o parte din acestea a fost înmuiată în soluţie de hiosciamozide cu concentraţia de 0,005% timp de 15 min (invenţia propusă). Pentru comparaţie, cealaltă parte de seminţe a fost înmuiată în soluţie de 0,01% a preparatului moldstim timp de 15 min (cea mai apropiată soluţie). În calitate de martor au servit seminţele înmuiate în apă. Seminţele din toate variantele după înmuiere au fost uscate şi ulterior semănate în câmp cu ajutorul semănătoarei pentru legume. Suprafaţa parcelei a constituit 364 m2. Suprafaţa de evidenţă a experienţei －1092 m2. Experienţa a fost efectuată în 4 repetiţii. In order to determine the germination of carrot seeds in the open field, part of them was soaked in a solution of hyoscyamoside with a concentration of 0.005% for 15 min (the proposed invention). For comparison, the other part of the seeds was soaked in a 0.01% solution of the moldstim preparation for 15 min (the closest solution). Seeds soaked in water served as a control. Seeds of all variants after soaking were dried and subsequently sown in the field using a vegetable seeder. The area of the plot was 364 m2. The area of the experiment - 1092 m2. The experiment was carried out in 4 repetitions.
La un interval de 9 zile după semănat în câmp s-a efectuat evidenţa germinaţiei. Evidenţele şi observările efectuate demonstrează că numărul de seminţe germinate, tratate cu soluţia de hiosciamozide, depăşeşte cu 112,5% varianta luată în calitate de martor şi cu 51,8% － varianta în care pentru înmuiere a fost utilizat preparatului moldstim (tabelul 2). Concomitent menţionăm faptul că tratarea seminţelor de morcov cu soluţia apoasă de hiosciamozide a favorizat germinaţia asigurând astfel apariţia şi dezvoltarea uniformă a plantulelor. Efectul în cauză a influenţat pozitiv asupra calităţilor comerciale ale producţiei. At an interval of 9 days after sowing in the field, germination was recorded. The records and observations made demonstrate that the number of germinated seeds treated with the hyoscyamoside solution exceeds by 112.5% the variant taken as a control and by 51.8% the variant in which the moldstim preparation was used for soaking (table 2). At the same time, we mention that the treatment of carrot seeds with the aqueous solution of hyoscyamoside favored germination, thus ensuring the appearance and uniform development of seedlings. This effect had a positive influence on the commercial qualities of the production.
Tabelul 2 Table 2
Influenţa glicozidelor asupra germinaţiei seminţelor de morcov în câmp deschis The influence of glycosides on the germination of carrot seeds in the open field
Varianta Germinaţia în câmp deschis, buc./m3 % faţă de martor Martorul 120 Moldstim (0,01%) 168 40,0 Hiosciamozide (0,005%) 255 112,5Variant Germination in open field, pcs./m3 % compared to control Control 120 Moldstim (0.01%) 168 40.0 Hyosciamoside (0.005%) 255 112.5
Aşadar, procedeul propus în comparaţie cu cea mai apropiată soluţie este mult mai efectiv, substanţa utilizată pentru tratare este obţinută din seminţele plantei Hyoscyamus niger L. care se găseşte în cantităţi suficiente în flora spontană. Toate acestea denotă avantajul procedeului propus faţa de soluţia proximă. Therefore, the proposed process in comparison with the closest solution is much more effective, the substance used for treatment is obtained from the seeds of the plant Hyoscyamus niger L. which is found in sufficient quantities in the spontaneous flora. All this denotes the advantage of the proposed process over the closest solution.
1. MD 365 Y 2011.05.31 1. MD 365 Y 2011.05.31
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20140053A MD830Z (en) | 2014-04-18 | 2014-04-18 | Process for pre-sowing treatment of carrot seeds |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20140053A MD830Z (en) | 2014-04-18 | 2014-04-18 | Process for pre-sowing treatment of carrot seeds |
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| MD830Y MD830Y (en) | 2014-11-30 |
| MD830Z true MD830Z (en) | 2015-06-30 |
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| MDS20140053A MD830Z (en) | 2014-04-18 | 2014-04-18 | Process for pre-sowing treatment of carrot seeds |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD791F1 (en) * | 1996-01-30 | 1997-07-31 | Univ De Stat Din Moldova | Process for silver recovery from the exhausted photographic material |
| MD1163G2 (en) * | 1997-07-02 | 1999-10-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Process for presowing carrots seeds treetment |
| MD2892G2 (en) * | 2005-04-06 | 2006-06-30 | Институт Физиологии Растений Академии Наук Республики Молдова | Trioside 3-O-{[b-D-glucopyranosyl(1 4)]-[b -D-glucopyranosyl(1 3)]- b-D-galactopyranoside}-(25R)-5a-furostan-3b,22a,26-triol-[26-O-b-D-glucopyranoside] - regulator of plant water exchange |
| MD2924G2 (en) * | 2005-04-06 | 2006-07-31 | Институт Физиологии Растений Академии Наук Республики Молдова | process for vine treatment |
| MD3738G2 (en) * | 2008-05-21 | 2009-06-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Tetraoside of (25R)-5α-furostan-3β-22α-26-triol-[26-O-β-D-glucopyranoside] as compound increasing the seed distant hybridization setting |
| EA013410B1 (en) * | 2005-06-16 | 2010-04-30 | Басф Акциенгезельшафт | Use of phenylsemicarbazones for seed treatment |
| MD365Y (en) * | 2011-01-10 | 2011-05-31 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of carrot seeds |
-
2014
- 2014-04-18 MD MDS20140053A patent/MD830Z/en not_active IP Right Cessation
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD791F1 (en) * | 1996-01-30 | 1997-07-31 | Univ De Stat Din Moldova | Process for silver recovery from the exhausted photographic material |
| MD1163G2 (en) * | 1997-07-02 | 1999-10-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Process for presowing carrots seeds treetment |
| MD2892G2 (en) * | 2005-04-06 | 2006-06-30 | Институт Физиологии Растений Академии Наук Республики Молдова | Trioside 3-O-{[b-D-glucopyranosyl(1 4)]-[b -D-glucopyranosyl(1 3)]- b-D-galactopyranoside}-(25R)-5a-furostan-3b,22a,26-triol-[26-O-b-D-glucopyranoside] - regulator of plant water exchange |
| MD2924G2 (en) * | 2005-04-06 | 2006-07-31 | Институт Физиологии Растений Академии Наук Республики Молдова | process for vine treatment |
| EA013410B1 (en) * | 2005-06-16 | 2010-04-30 | Басф Акциенгезельшафт | Use of phenylsemicarbazones for seed treatment |
| MD3738G2 (en) * | 2008-05-21 | 2009-06-30 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Tetraoside of (25R)-5α-furostan-3β-22α-26-triol-[26-O-β-D-glucopyranoside] as compound increasing the seed distant hybridization setting |
| MD365Y (en) * | 2011-01-10 | 2011-05-31 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of carrot seeds |
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| Publication number | Publication date |
|---|---|
| MD830Y (en) | 2014-11-30 |
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| FG9Y | Short term patent issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) |