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MD4205C1 - Method for determining the activity of catalase - Google Patents

Method for determining the activity of catalase

Info

Publication number
MD4205C1
MD4205C1 MDA20120004A MD20120004A MD4205C1 MD 4205 C1 MD4205 C1 MD 4205C1 MD A20120004 A MDA20120004 A MD A20120004A MD 20120004 A MD20120004 A MD 20120004A MD 4205 C1 MD4205 C1 MD 4205C1
Authority
MD
Moldova
Prior art keywords
catalase
activity
determining
phosphate buffer
mixture
Prior art date
Application number
MDA20120004A
Other languages
Romanian (ro)
Russian (ru)
Other versions
MD4205B1 (en
Inventor
Надежда ЕФРЕМОВА
Агафия УСАТЫЙ
Елена МОЛОДОЙ
Original Assignee
Институт Микробиологии И Биотехнологии Академии Наук Молдовы
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Институт Микробиологии И Биотехнологии Академии Наук Молдовы filed Critical Институт Микробиологии И Биотехнологии Академии Наук Молдовы
Priority to MDA20120004A priority Critical patent/MD4205C1/en
Publication of MD4205B1 publication Critical patent/MD4205B1/en
Publication of MD4205C1 publication Critical patent/MD4205C1/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to biotechnology, in particular to a method for determining the activity of catalase and can be used in biochemical research, as well as to control the technological process of catalase preparation in the microbiological industry.Summary of the method consists in that the biological material is introduced into a mixture containing: 0.05 M of phosphate buffer, 0.06% of hydrogen peroxide and 5 mM of ethylene diamine tetraacetic acid, then the mixture is incubated at the temperature of 25°C and is determined spectrophotometrically the optical density of the reaction product in relation to phosphate buffer solution, the measurements are performed at a wavelength of 240 nm at the beginning of the reaction and in 15 seconds, and the activity of catalase is defined in accordance with the established formula.
MDA20120004A 2011-12-26 2011-12-26 Method for determining the activity of catalase MD4205C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MDA20120004A MD4205C1 (en) 2011-12-26 2011-12-26 Method for determining the activity of catalase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MDA20120004A MD4205C1 (en) 2011-12-26 2011-12-26 Method for determining the activity of catalase

Publications (2)

Publication Number Publication Date
MD4205B1 MD4205B1 (en) 2013-02-28
MD4205C1 true MD4205C1 (en) 2013-09-30

Family

ID=47831226

Family Applications (1)

Application Number Title Priority Date Filing Date
MDA20120004A MD4205C1 (en) 2011-12-26 2011-12-26 Method for determining the activity of catalase

Country Status (1)

Country Link
MD (1) MD4205C1 (en)

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1399682A1 (en) * 1985-07-02 1988-05-30 В.И. Дудин Method of analysis of catalase in blood
SU1422121A1 (en) * 1987-01-21 1988-09-07 Институт Ботаники Ан Казсср Method of revealing catalase activity in biological objects
SU1436070A1 (en) * 1986-11-18 1988-11-07 Воронежский государственный университет им.Ленинского комсомола Method of determining catalase activity of soil
WO1990002202A1 (en) * 1988-08-16 1990-03-08 Cetus Corporation Reduction of peroxidatic and catalatic interference with assays of peroxidatic activity
RU2027171C1 (en) * 1991-07-05 1995-01-20 Узбекский научно-исследовательский институт каракулеводства Method of catalase activity assay in biological objects
WO2001034831A2 (en) * 1999-11-12 2001-05-17 Smithkline Beecham P.L.C. Determination of catalase activity
RU2175445C2 (en) * 1999-11-15 2001-10-27 Научно-исследовательский институт кардиологии Томского научного центра СО РАМН Incubation medium for determination of plasma blood catalase activity
RU2235320C1 (en) * 2003-03-13 2004-08-27 Московский государственный университет леса Method of determining catalase-catalyzed hydrogen peroxide degradation rate in soils
US20060199238A1 (en) * 2005-02-22 2006-09-07 Neil Charter ADP detection using an enzyme-coupled reaction
CN101464389A (en) * 2007-12-19 2009-06-24 苏州艾杰生物科技有限公司 Monoamine oxidase diagnostic reagent kit and method for measuring active concentration of monoamine oxidase

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1399682A1 (en) * 1985-07-02 1988-05-30 В.И. Дудин Method of analysis of catalase in blood
SU1436070A1 (en) * 1986-11-18 1988-11-07 Воронежский государственный университет им.Ленинского комсомола Method of determining catalase activity of soil
SU1422121A1 (en) * 1987-01-21 1988-09-07 Институт Ботаники Ан Казсср Method of revealing catalase activity in biological objects
WO1990002202A1 (en) * 1988-08-16 1990-03-08 Cetus Corporation Reduction of peroxidatic and catalatic interference with assays of peroxidatic activity
RU2027171C1 (en) * 1991-07-05 1995-01-20 Узбекский научно-исследовательский институт каракулеводства Method of catalase activity assay in biological objects
WO2001034831A2 (en) * 1999-11-12 2001-05-17 Smithkline Beecham P.L.C. Determination of catalase activity
RU2175445C2 (en) * 1999-11-15 2001-10-27 Научно-исследовательский институт кардиологии Томского научного центра СО РАМН Incubation medium for determination of plasma blood catalase activity
RU2235320C1 (en) * 2003-03-13 2004-08-27 Московский государственный университет леса Method of determining catalase-catalyzed hydrogen peroxide degradation rate in soils
US20060199238A1 (en) * 2005-02-22 2006-09-07 Neil Charter ADP detection using an enzyme-coupled reaction
CN101464389A (en) * 2007-12-19 2009-06-24 苏州艾杰生物科技有限公司 Monoamine oxidase diagnostic reagent kit and method for measuring active concentration of monoamine oxidase

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Некрасова Г.Ф., Киселёва И.С. УМКД "Экологическая физиология растений". Руководство к лабораторным и практическим занятиям. Уральский государственный университет, 2008, с. 157 *
Рахманова Т.И., Матасова Л.В., Семенихина А.В., Сафонова О.А., Макеева А.В., Попова Т.Н. Методы оценки оксидативного статуса. Учебно-методическое пособие для вузов. Издательско-полиграфический центр Воронежского государственного университета, 2009, с. 62 *

Also Published As

Publication number Publication date
MD4205B1 (en) 2013-02-28

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FG4A Patent for invention issued
KA4A Patent for invention lapsed due to non-payment of fees (with right of restoration)
MM4A Patent for invention definitely lapsed due to non-payment of fees