LU506831B1 - A MAINTENANCE PROCEDURE FOR SEALING BASEMENT SLUDGE - Google Patents

Abstract

The present invention discloses a maintenance method for sealing cellar sludge, which is prepared from the following weight of raw materials: the present invention relates to a cellar skin sludge maintenance method, belongs to the technical field of liquor brewing. Obtained by maintaining the cellar skin sludge feel wet, tender, oily, rubbing no roughness, smell has a distinct sour taste, no foreign smell or odor, microscopic examination of bacilli, clostridia more and fat.

Description

A maintenance procedure for sealing basement sludge LU506831

technical area

The present invention relates to the technical field of white wine production, in particular to a maintenance method for sealing cellar sludge.

technology in the background

The production of strong aroma liquor is a traditional solid fermentation, which is highly dependent on the cellar, and the quality of the cellar sludge in the cellar plays a very important role in the quality of the strong aroma liquor, because of the high quality of the old

Cellar mud in the growth of a variety of different functions beneficial to the

Brewery of microbial communities. One of the hexanoic acid bacteria is a type of

Clostridium difficile, a strong aroma of lye fermentation is a crucial role in a

Cellar sludge functional bacteria play, their number, how much and the function of the strength of fermentation of strong aroma of liquor directly affect the quality of Daqu wine fermentation, because its metabolism of hexanoic acid and the fermentation products of alcohol to produce

Ethylhexanoate, the main aroma of strong aroma of Daqu wine components. In the fermentation of strong aroma liquor production, the fermentation of grains in addition to the bottom of the

Cellar mud and basement wall contact, but also with the basement waterproofing of the basement

Skin mud contact. If in accordance with the basement depth of 2m, basement cap

Height of 0.5 m, basement length of 3m width of 2m rectangular basement calculations that

Basement floor mud area of 6m3 Basement wall mud area of 20m°*. Basement skin

Mud area of 11m*.

In brewing production, after the processed grains have been brought into the cellar, the surface of the yeast sediment is usually covered with 8-12 cm thick cellar sludge.

After sealing the basement, the mud is smoothed and polished, and then the basement is cleaned once a day. If the basement mud is cracked due to the subsidence of fermented bad spirits, it should be smoothed in time until it is formed and no longer cracked, and then a layer of plastic film should be placed over the mud to prevent the basement mud from drying and cracking. The purpose of sealing the

The purpose of sealing the cellar with sealing mud is to isolate the grapes from the outside air, which creates anaerobic conditions and prevents the entry of harmful microorganisms, while at the same time preventing the yeast in the air from releasing a large amount of fermentable

Sugar is consumed to ensure that the fermentation of the wine proceeds normally. If the

If the cellar is not sealed, e.g. if the cellar roof is leaking, this leads to rot, mold, acidity and a bad taste of the wine. The sealing mud is also part of the

Cellar sludge. Production practice shows that the cellar sludge not only has a cellar sealing effect, but also has a close relationship with the upper layer of poor wine quality, and the number of microbial populations on the upper layer of poor wine quality plays an important role. At present, people do the maintenance of cellar

Sludge is mainly concentrated on the basement floor mud and basement wall mud, and the lack of effective maintenance of the basement skin mud, only the basement skin mud as a sealing effect, no consideration of the functional microbial nutrition and

Metabolism, so that the basement skin mud composition and function is simple, mainly in the main function of the bacterium caproic acid bacteria insufficient number.

Therefore, the present invention requires the development of new maintenance methods for the

Sealing of cellar sludge that meets the requirements of wine production.

Content of the invention LU506831

The main purpose of the present invention is to provide a maintenance method for the

Sealing of the basement sludge in response to the problems that the general traditional basement sludge consists mainly of yellow sludge, which in its

Composition and function is simple and has less impact on the quality and

taste characteristics of strongly aromatic white wine, and the characteristics of

cellar sludge, which can be easily exchanged and moved, is not fully utilized. The cellar sludge produced by the process is rich in hexanoic acid bacteria, which can effectively improve the quality and taste characteristics of strong aromatic white wine and increase the proportion of high-quality products of strong aromatic large-volume wine.

To achieve the above purpose, the present invention adopts the following technical solutions:

Advantageous effect of the present invention:

The preservation method of the cellar skin sludge of the present invention can not only avoid the deterioration of the quality of the cellar skin sludge, but also

Improve the quality of cellar skin sludge. The present invention provides a new method for the preservation of cellar skin sludge, which has a wide application prospect.

Won maintenance basement skin mud feel wet, tender, oily, rubbing no roughness,

Odor of more obvious hexanoic acid, no foreign odor or foreign odor, microscopic examination of bacilli, clostridia more and fat.

Description of the attached drawings

Figure 1 is a schematic representation of the process flow of the present invention.

Detailed Description (1) Expanded Culture of Capric Acid Bacteria Seeds: (I) Primary Seed Culture:

Refrigerator storage of liquid strains by 80 °C treatment for 10min, after 10% of

Inoculum into the liquid volume of 100mL 100mL triangular flask, cultured at 35 °C for 7d to obtain the first level of seed standby; @) Secondary seed culture: the first stage of seed liquid corresponding to 10% of the inoculum into the liquid volume of 1L 1L

Triangular flask, cultured at 35 °C for 7 days to obtain the second stage of seed readiness; ©)

Tertiary seed culture: the second stage of seed liquid corresponding to 10% of the inoculum in the liquid volume of 10L of the fermenter, at 35 °C culture of the third stage of

Seed readiness 7d;@ Fourth-class seed culture: the third-class seed liquid was connected to the fermenter with a liquid volume of 100L corresponding to the inoculum amount of 10% and cultured at 35°C for 7d to obtain the fourth-class seeds; ©)

Adopting the same method, and then the fourth-class seed liquid was amplified by 10 times to obtain the fifth-class seed liquid of 1m3. (2) Preparation of hexanoic acid bacteria culture liquid: the material according to the

Prepare the formula of the hexanoic acid bacteria culture medium, sterilize, add ethanol when

Cool to 35°C, then add the five-stage seed liquid at a ratio of 10% into the 10m* fermenter, stir well and then close the stirrer and culture at 35°C for 7d.

The capric acid bacteria strain in (1) and (2) refers to Clostridium perfringens with the signature Clostridium, species name Kluyver; the capric acid bacteria medium contains the following individual components and contents: CH3COONa 0.5%, K2HPO4 0.04%, MgSO4.7H20

0.02%, (NH4)2804 0.1%, yeast extract 0.1%. CaCO3 0.05%, wine lees 10%, ethanol 2%. Dk4/506831

Medium was sterilized at 121 °C for 30 minutes, and ethanol was added before inoculation. (3) Detection of bacterial caprose liquid: After fermentation, the indicators of bacterial caprose liquid produced are as follows: light yellow appearance; slightly caprose-like odor; pH=5.5; number of caprose bacteria=1.1<10*/mL. (4) Collection and sealing of cellar sludge: After the completion of fermentation of the bad spirit, the cellar skin is cut into small

Squares cut and loaded into the sludge hopper; then the cellar sludge is transported to the yard of the cellar sludge treatment workshop. (5) Ingredient Mixing: the prepared to the cellar skin mud raking to a slightly higher casing, which in turn scattered into the cellar skin mud ingredients, cellar skin

Mud maintenance ingredients from the cellar skin to 48% of the mud, 5% of the large

Currant powder; 5% of soybean cake powder; 5% of bran; said (2) in the

Hexanoic acid bacterial culture liquid 10%; wine tail 15%; 95% alcohol 2%; 10% of high-quality yellow water; and then the first coarse mixture. After the end of the coarse

Mixing, start the power button of the basement mud machine, send the mixture into the inlet of the mud whirlwind with a moving bucket, and let the mud

Cyclone to fully mix it until it is evenly kneaded, and in the mixing process, it is necessary to ensure that all kinds of raw materials are uniform and permeable, and to control the water content of the basement skin mud than 40%. (6) Cultivation of basement sludge: After the mixing of the material is completed, use the holding bucket to make good basement sludge, which is transported to the cement basin sealed with a film to cultivate under natural conditions for 30d.

After maintaining the cellar skin mud should be moist, tender, oily, rub no

Roughness, smell of obvious hexanoic acid, no foreign smell or odor, microscopic examination of rod, clostridial bacteria and grease. (7) Cellar skin mud application: after the completion of the culture, the holding bucket will be cellar skin mud into the transport vehicle, transported to the cellar side to

Cellar scum of grains in the cellar after entering the cellar, the cellar pool cellar

Skin mud collection, wipe the surface with mud, without damage to the

Sealing the plastic film, the fermentation time of 70d.

The basement sludge after maintenance of the present invention was used to

To seal the cellar of a strongly flavored white wine, and in addition the cellar was sealed with used and unmaintained cellar sludge, and the quality of the

fermentation of pasta yeast wine was compared and the results are presented in Table 1.

Table 1 Comparison of the cellar sludge after treatment with the present

invention with the untreated basement sludge

. . . . . U506831

Cellar sludge | Wet, tender, oily, no roughness, clear | Typical strong mm after maintenance | sour taste, no | Aroma style, elegant of invention mold spots on the surface, many | Cellar sludge aroma, and thick rod-shaped and clostridial | no mixed taste,

Bacteria at microscopic | slightly sweet

Examination. Taste, excellent

wine quality.

Unpreserved hard clay lumps, dry cracking phenomenon, | With mud smell,

Cellar floor the surface of the breeding white | bitter taste,

Mold spots that have a bad smell | heavy, microscopic examination of the | mixed taste,

rods, clostridia few and small and | poor wine quality lean

As can be seen from Table 1, the unpreserved cellar sludge exhibited uniform, hard sludge lumps, which is due to the uneven mixing of the soil and the

drying out into lumps. After sealing the cellar with the unpreserved cellar sludge, numerous cracks appeared on the surface of the cellar, white mold spots grew on the surface, which bore a foul odor, few and thin rod-shaped and clostridial bacteria under microscopic examination, and the obtained pasta lees wine had a muddy odor, bitter taste and various flavors, and the wine quality was poor. The quality of the cellar sludge obtained by applying the present invention is significantly higher than that of the unpreserved cellar sludge, and the quality of the obtained pasta lees wine also has an elegant cellar skin aroma and better wine quality.

Claims (7)

claims LU506831 1. A maintenance method for sealing basement sludge, characterized in that it comprises the following steps: 5 (1) After expanding the culture of hexanoic acid bacteria strain, preparing the expanded culture seed liquid of hexanoic acid bacteria; (2) In a 10m? fermentation tank, according to the hexanoic acid bacteria culture medium formula, materials are prepared, sterilized, cooled to about 35 °C, add ethanol (? as much as possible), and then to the proportion of about 10% of the hexanoic acid bacteria expanding culture seed liquid, mix well, and then static cultivation at 30-37 °C for 5-11d to obtain hexanoic acid bacteria culture liquid; (3) Collect the sealed cellar skin mud to be retained as cellar skin mud, and according to a certain proportion to the (2) add in the caproic acid bacterial culture liquid, large currant powder, soybean cake powder, bran, wine tails, about 95% alcohol and yellow water, etc. to make the cellar skin mud maintenance ingredients mixture, the mixture through the churning machine to fully mix until evenly kneaded, and the mixture of water content of 30-50%, to obtain the processed cellar skin mud;N (4) after the completion of mixing of uniform, the resulting treated cellar skin mud transported to the cement pool, sealed with a film to cultivate under natural conditions for 10-40d, that is, to preserve. 2. A maintenance method for sealing cellar sludge according to claim 1, characterized in that: said method for expanding the culture of hexanoic acid bacteria seeds is as follows: D the refrigerator preservation of liquid bacterial species by 75-85 °C treatment for about 10min corresponding to about 10% of the inoculum amount into the filling volume of 100mL 100mL triangular flasks, in 30-37 °C cultivation of about 7d to obtain the first stage of seed liquid readiness; © The first quality seed liquid was connected to a 1L triangular flask with a liquid volume of 1L in accordance with an inoculum volume of about 10% and cultured at 30-37 °C for about 7d to obtain the second quality seed liquid for reserve; © The second quality seed liquid was connected to a fermenter with a liquid volume of 10 | connected in accordance with an inoculum amount of about 10% and cultured at 30-37°C for 5-11 days to obtain the third-class seed liquid; @ Introduce the third-class seed liquid in accordance with an inoculum amount of about 10% into the fermenter with the liquid volume of 100L and culture at 30-37°C for about 7d to obtain the fourth-class seed liquid for reserve; © Connect the fourth-class seed liquid corresponding to the inoculum amount of about 10% to the fermenter with the liquid volume of 1000L and culture at 30- 37°C for about 7d to obtain the fifth-class seed liquid, that is, the seed liquid of the expanded culture of capric acid bacteria; 3. A maintenance method for sealing basement sludge according to claim 1, dadurdy/506831, characterized in that the strain of capric acid bacteria is Clostridium perfringens with the signature Clostridiun, species name Kluyver. 4. A maintenance method for sealing basement sludge according to claim 1, characterized in that: the culture medium for hexanoic acid bacteria contains various components and their contents are: CH3COONa 0.5% or so, K2HPO4 0.04% or so, MgSO4.7H20 0.02% or so, (NH4)2S04 0.1%. Yeast extract 0.05-0.5% or so, CaCO3 0.05% or so, wine tail 1-20%, ethanol 1-5%, (?). The above culture medium components together are not equal to 100 percent, the remaining amount must be why to write the substance); the sterilization of said hexanoic acid bacteria culture medium is sterilized at about 121 °C for about 30 min. 5. A maintenance method for sealing basement sludge according to claim 1, characterized in that: The individual components and contents contained in the hexanoic acid bacteria medium are: CH3COONa 0.5%, K:HPOs 0.04%, MgSO4.7H20 0.02%, (NH4)2S04 0.1%, yeast extract 0.05-0.5%, CaCO3 0.05%, vine tail 1-20% and ethanol 1-5%. 6. Fin maintenance method for sealing basement sludge according to claim 1, characterized in that: The method for collecting basement sealing sludge is as follows: when the fermentation of bad alcohol is finished, a small square spade is used to cut the basement skin into small squares and load them into the sludge hopper, and then the basement skin sludge is transported to the yard of the basement skin sludge treatment workshop. 7. A maintenance method for sealing cellar sludge according to claim 1, characterized in that the mixture of ingredients for preserving the cellar sludge consists of the following components (in weight percent): 25-80% of the cellar sludge to be preserved, 1-10% of the dacquoise powder; 1-10% of the soybean cake powder; 1-10% of the bran; 1-10% of the hexanoic acid bacterial culture solution obtained in step (2); 1-20% of the vine tail; 1-5% of the 95% alcohol; 1-20% of the yellow water;