LU503155B1

LU503155B1 - Plant Essential Oil Insect Repellent for Edible Fungi and Its Preparation Method

Info

Publication number: LU503155B1
Application number: LU503155A
Authority: LU
Inventors: Hualin Hu; Chao Sun
Original assignee: The Key Laboratory Of Chemistry For Natural Products Of Guizhou Province And Chinese Academy Of Scie
Priority date: 2022-12-07
Filing date: 2022-12-07
Publication date: 2023-06-07

Abstract

The invention discloses a plant essential oil insect repellent for edible fungi. And the insect repellent consists of the following components: 12-18 parts of peppermint oil, 15-20 parts of navel orange oil, 20-35 parts of lemon oil, 8-15 parts of citronella oil, 13-20 parts of scutellaria baicalensis extract, 3-8 parts of tobacco leaves, 6-10 parts of stemona and 10-20 parts of emulsifier. The preparation method comprises the following steps: firstly, make tobacco leaves and Stemona into distillate medicine liquid, then add peppermint oil, navel orange oil, lemon oil and citronella oil into emulsifier, seal and stir, then add scutellaria baicalensis extract, then put them into a distiller with a pressure of 90-150MPa and a temperature of 100-120oC, finally put the liquid into a homogenizer, stir at 60-70oC for half an hour, and cool to room temperature to obtain the finished product.

Description

DESCRIPTION LU503155

Plant Essential Oil Insect Repellent for Edible Fungi and Its Preparation Method

TECHNICAL FIELD

The invention belongs to the technical field of bactericides and insect repellents, in particular to a plant essential oil insect repellent for edible fungi and its preparation method.

BACKGROUND

Edible fungi are favored by consumers because of their delicious taste, rich in nutrients such as protein, vitamins, dietary fiber, minerals and amino acids, and high edible and medicinal value.

With the increase of people's demand for edible fungi, the planting scale of edible fungi is increasing, and the industrial scale is also expanding. Due to the expansion of production scale and planting technology, the pests and diseases become more and more rampant in the planting process, which has become a serious problem limiting the yield and quality of edible fungi.

According to statistics, there are more than 90 kinds of common pests and more than 30 kinds of harmful mites in the process of planting edible fungi. Every year, the yield of edible fungi is reduced by 20%-30% due to pests, and even the production of edible fungi is completely lost. Therefore, the effective control of edible fungi pests is the key to improve the yield and quality of edible fungi.

Mosquitoes and flies are the most common pests in the process of planting edible fungi, because of their strong reproductive ability, and both larvae and adults can cause damage to edible fungi. This kind of pests mostly grow in damp and shady areas, and feed on damp and rotten food. Its living environment is highly compatible with the planting environment of edible fungi, and it is one of the most common and difficult to clear pest species. Larvae usually feed on the mycelium of edible fungi, which causes the decay effect on edible fungi, leading to yellowing, atrophy, mildew and death of edible fungi. And drosophila melanogaster is especially typical in the cultivation of Pleurotus geesteranus and Agrocybe aegerita.

At present, in the process of planting edible fungi, growers use a large number of pesticides to control pests. Although this method has achieved certain results in a short period of time, long-term use or excessive use will make pests resistant, and the continued use of pesticides will not achieve the effect of pest control. On the other hand, the heavy use of pesticides causes serious pollution of edible fungi, which threatens the nutrition and food safety of edible fung; 503155

How to control the pests and diseases of edible fungi without pollution, improve the yield and quality of edible fungi, reduce the production cost and improve the economic benefits of production has become a big problem to limit the scale of edible fungi industry.

SUMMARY

In order to solve the above technical problems, the present invention provides a plant essential oil insect repellent for edible fungi and its preparation method.

In order to achieve the above technical purpose, the technical scheme used is as follows:

The plant essential oil insect repellent for edible fungi, and the insect repellent consists of the following components in parts by weight: 12-18 parts of peppermint oil, 15-20 parts of navel orange oil, 20-35 parts of lemon oil, 8-15 parts of citronella oil, 13-20 parts of scutellaria baicalensis extract, 3-8 parts of tobacco leaves, 6-10 parts of stemona and 10-20 parts of emulsifier.

In the preferred technical scheme of the invention, the emulsifier is made of Tween -20 and/or octylphenol polyoxyethylene ether.

In the preferred technical scheme of the invention, the insect repellent consists of the following components in parts by weight: 15 parts of peppermint oil, 18 parts of navel orange oil, parts of lemon oil, 10 parts of citronella oil, 15 parts of scutellaria baicalensis extract, 5 parts of tobacco leaves, 8 parts of stemona and 15 parts of emulsifier.

In the preferred technical scheme of the invention, the insect repellent consists of the following components in parts by weight: 16 parts of peppermint oil, 17 parts of navel orange oil, parts of lemon oil, 12 parts of citronella oil, 18 parts of scutellaria baicalensis extract, 6 parts of tobacco leaves, 7 parts of stemona and 13 parts of emulsifier.

Another purpose of the present invention is to provide a plant essential oil insect repellent for edible fungi. And it comprises the following steps: (1) weigh each component according to that weight parts of each raw material for later use; (2) respectively crush the tobacco leaves and the stemona into 50-100 meshes particles; mix the crushed tobacco with the mashed stemona, add water and soak in water for 15-20min, the added water is 1-3 times of the total mass of tobacco leaves and stemona, distill, and collek#503155 distillate to obtain liquid medicine for later use; (3) mix peppermint oil, navel orange oil, lemon oil and citronella oil, add emulsifier, seal, heat and stir for 25-40min, and control the temperature at 30-40°C; (4) after mixing the materials in step (2) and step (3) and scutellaria baicalensis extract, place the mixture in a distiller and inject steam with a pressure of 90-150MPa; and control the temperature of steam at 100-120°C; (5) stir the liquid in step (4) in a homogenizer, keep the temperature at 60-70°C and stir for half an hour, and cool it to room temperature to obtain the finished product.

In the preferred technical scheme of the invention, the stirring speed of the homogenizer 1s 20-50m/s.

Another purpose of the present invention 1s to provide the application of the insect repellent in medicine for preventing and controlling the drosophila melanogaster of Pleurotus geesteranus and Lentinus edodes.

Another purpose of the present invention 1s to provide the application of the insect repellent in the medicine for preventing and controlling pests and harmful mites of Agrocybe aegerita and

Flammulina velutipes.

Another purpose of the present invention 1s to provide the application of the insect repellent in the medicine for preventing and controlling the diseases of edible fungi.

The inventor found that lemon oil and citronella oil added with scutellaria baicalensis extract can stimulate the pheromone of drosophila melanogaster, stimulate the brain system of drosophila melanogaster to produce chaos, change its adaptability to photoperiod, temperature and humidity, and achieve the effect of luring it to eat and mate. The scutellaria baicalensis extract has inhibitory effect on miscellaneous bacteria, and has no effect on normal mycelia. The lemon oil, citronella oil and scutellaria baicalensis are not easy to produce drug resistance to pests, and have no phytotoxicity to edible fungi.

Tobacco leaves and stemona have insecticidal and lice killing effects, and they have a good repelling effect on pests such as mosquitoes, flies, noctuids, red spiders and nematodes in the cultivation of edible fungi. Plant essential oil, tobacco leaves, stemona and scutellaria baicalensis extract can well inhibit the rotten rate of fungus sticks, and reduce the probability of edible fungi mycelia atrophy and mildew. LU503155

The invention has the beneficial effects that: (1) The insect repellent of the invention, especially lemon oil, citronella oil, scutellaria baicalensis extract and other components, has a good insect repellent effect on Pleurotus geesteranus and Agrocybe aegerita, and has an obvious inhibition effect on rotten sticks caused by drosophila melanogaster. The insect repellent can reduce the harm of drosophila melanogaster to Pleurotus geesteranus and Agrocybe aegerita, reduce the planting cost and increase the yield, and has a good application prospect. (2) The insect repellent of the invention can effectively prevent and control the influence of pests and miscellaneous bacteria on mycelia in the growth process of edible fungi, so as to effectively inhibit the occurrence of pests and diseases of edible fungi; The insect repellent has no drug resistance, no pollutant residue in use, no harm to people and the environment, and can be used continuously, meeting the food safety requirements. However, the existing fungicides, in the process of continuous application, bacteria and insect pests are resistant, so it is necessary to double the use to produce the effect, and in the process of application, pesticide residues are serious, endangering food safety. (3) The insect repellent of the invention not only has high killing rate, but also has stable drug effect, long time effect, safe and non-toxic raw materials, no residue after application, no pollution. In addition, the production method of the insect repellent is simple, easy to operate, low in production cost and convenient to use. The application of the insect repellent can effectively reduce the rotten rate of the sticks and improve the yield and quality of edible fungi.

DETAILED DESCRIPTION OF THE INVENTION

The scheme of the present invention will be further described in detail with reference to the following embodiments. The following description is only to explain the present invention, but not to limit its content.

Embodiment 1 12kg of peppermint oil, 15kg of navel orange oil, 20kg of lemon oil, 8kg of citronella oil, 13kg of scutellaria baicalensis extract, 3kg of tobacco leaves, 6kg of stemona and 10kg of Tween -20; And the preparation method is as follows:

(1) Weigh each component according to that weight parts of each raw material for later usd;J203155 (2) Respectively crush the tobacco leaves and the stemona into 50-100 meshes particles; mix the crushed tobacco with the mashed stemona, add water and soak in water for 15min, the added water is 2 times of the total mass of tobacco leaves and stemona, distill, and collect distillate to obtain liquid medicine for later use; (3) Mix peppermint oil, navel orange oil, lemon oil and citronella oil, add Tween -20, seal, heat and stir for 40min, and control the temperature at 30°C; (4) After mixing the materials in step (2) and step (3) and scutellaria baicalensis extract, place the mixture in a distiller and inject steam with a pressure of 150MPa; and control the temperature of steam at 100°C; (5) Stir the liquid in step (4) in a homogenizer, keep the temperature at 70°C and stir for half an hour, and cool it to room temperature to obtain the finished product.

Embodiment 2 18kg of peppermint oil, 20kg of navel orange oil, 35kg of lemon oil, 15kg of citronella oil, 20kg of scutellaria baicalensis extract, 8kg of tobacco leaves, 10kg of stemona and 20kg of octylphenol polyoxyethylene ether; And the preparation method is as follows: (1) Weigh each component according to that weight parts of each raw material for later use; (2) Respectively crush the tobacco leaves and the stemona into 50-100 meshes particles; mix the crushed tobacco with the mashed stemona, add water and soak in water for 20min, the added water is 1 time of the total mass of tobacco leaves and stemona, distill, and collect distillate to obtain liquid medicine for later use; (3) Mix peppermint oil, navel orange oil, lemon oil and citronella oil, add octylphenol polyoxyethylene ether, seal, heat and stir for 25min, and control the temperature at 40°C; (4) After mixing the materials in step (2) and step (3) and scutellaria baicalensis extract, place the mixture in a distiller and inject steam with a pressure of 90MPa; and control the temperature of steam at 120°C; (5) Stir the liquid in step (4) in a homogenizer, keep the temperature at 60°C and stir for half an hour, and cool it to room temperature to obtain the finished product.

Embodiment 3 15kg of peppermint oil, 18kg of navel orange oil, 25kg of lemon oil, 10kg of citronella oil,

15kg of scutellaria baicalensis extract, Skg of tobacco leaves, 8kg of stemona, 7kg Tween 3503155 and 8kg of octylphenol polyoxyethylene ether. And the preparation method is same as the

Embodiment 1.

Embodiment 4 16kg of peppermint oil, 17kg of navel orange oil, 30kg of lemon oil, 12kg of citronella oil, 18kg of scutellaria baicalensis extract, 6kg of tobacco leaves, 7kg of stemona, Skg Tween -20 and 8kg of octylphenol polyoxyethylene ether. And the preparation method is same as the

Embodiment 1.

Application example

Experiment 1

Add 2000g of water into 10g of the product prepared in Embodiments 1-4; In Comparative example 1, the insect-proof agent purchased by Suizhou Ligiang Biotechnology Development

Co., Ltd. of Hubei Province is used, and add 2000g of water into 10g of the insect-proof agent;

Spray 2,000g water on the Comparative example 2; Experiments are conducted in Pleurotus geesteranus fields provided by Yunyi Edible Fungi Cooperative and Longhui Edible Fungi

Cooperative. The cultivation formula of edible fungi is as follows: 75% corn cob, 10% cottonseed hull, 10% bran and 5% lime. The fungus sticks are made of 22x50x0.03cm low-pressure polyethylene plastic bags, with the ratio of material to water of 1:1.3, add 2.0kg of wet materials per stick according to the ratio of material to water, and the material height is 24cm and the dry material weight of the stick is 0.87kg.

Pleurotus geesteranus clinker cultivation, atmospheric pressure space bag sterilization, and the experiment was inoculated on May 1, 2021, sprayed with insecticides on May 16, and fruiting on May 23. Keep 60%-70% humidity during the fungus growth management of

Pleurotus geesteranus in the high temperature period, and keep fresh air circulation in the greenhouse from the beginning to the end to prevent neurosporacrasa pollution; After the sticks are full of mycelium, remove the collar newspaper, spray water to keep the humidity of mushroom room at 85%-90%, collect and weigh when the mushroom cover is 3-4cm, and remove the residual mushroom roots and dead mushroom buds on the material surface in time to prevent the parasitism of drosophila melanogaster. Table 1 shows the results of insect repellent on the drosophila melanogaster of Pleurotus geesteranus. Table 2 shows the investigation results of insect repellent on the number of Pleurotus geesteranus rotten sticks. And Table 3 shows th¢/203155 survey results of insect repellent on Pleurotus geesteranus yield.

The results in Table 1 show that compared with the Comparative example only sprayed with water, the products of Embodiments 1-4 and the commercially available insect-proof agent can achieve better insect repellent effect, and there is no difference in insect repellent effect between

Embodiments 1-4 of the present invention and the commercially available insect-proof agent. In addition, the insect repellent of the present invention is stable in efficacy and long in time, and the insect repellent of Embodiments 1-4 can effectively repel drosophila melanogaster.

Table 1 Results of insect repellent on the drosophila melanogaster of Pleurotus geesteranus

Number 5/16 Hang 5/28 6/2 6/7 6/17 | 6/1 | 6/30 | 7/30 | 7/1 yellow Change Second Chang | (hea | 9(h | (hea | (hea | 7(h board(head) yellow spraying e d) |ead)| d) d) | ead board(h yellow ) ead) board( head) 5/ | 5/2 | 5/28 | 5/30 | 6/2 | 6/4 | 6/7 | 6/8 | 6/1 17] 2 2 faioimeni [5/99 [1 o [9 6 MB 0 [on | | [0 enna 3 on [6 [m [Mm [wi enna 53 ww [0 [m [a [70 0s 9 wm oa m sans [516 | ww [5 [0 [woo

Comparative 78 82 63 88 59 79 | 40 | 64 102 | 106 | 129 | 156 example 1

Comparative | 50 | 220 | 286 | 106 | 289 210 | 52 | 156 | 286 | 307 | 324 | 366 | 476 example 2

In Table 2, the results of the investigation on the number of Pleurotus geesteranus rotten sticks show that Embodiments 1-4 can effectively reduce the rotten sticks number of Pleurotus geesteranus, and reduce the rotten sticks rate of Pleurotus geesteranus compared with insect-proof agent and water.

Table 2 Investigation of insect repellent on the number of Pleurotus geesteranus rotten -4J503155 sticks

Embodiment 5/1 1 3 5 5 -54.54 1

Embodiment 5/1 2 3 3 -72.72 2

Embodiment 5/1 3 3 4 -63.64 3

Embodiment 5/1 1 4 5 5 -54.54 4

Comparative 5/1 1 1 7 7 13 +18.18 example 1

Comparative 5/1 1 1 11 example 2

In Table 3, the yield test of Pleurotus geesteranus shows that all the Embodiments 1-4 have high yield, which is slightly higher than that of insect-proof agent and about 10% higher than that of pure water.

Table 3 Comparison of insect repellent on Pleurotus geesteranus yield.

Embodiment 5/24 7/15 5.87 3.80 1.84 1.16 12.67 1

Embodiment 5/24 7/17 5.09 3.37 1.80 1.23 11.49 2

Embodiment 5/24 7/17 5.15 3.78 1.98 1.26 12.17 3

Embodiment 5/25 7/17 5.25 3.43 1.53 1.06 11.27 4

Comparative 5/24 7/14 5.29 3.42 1.31 1.05 11.07 example 1

Comparative 5/24 7/17 4.97 3.07 1.30 1.03 10.37 example 2

Experiment 2

From July 1 to 10, 2021, 100 sticks of Lentinus edodes are treated with the insecticides (the insecticides treatment method is the same as that of Experiment 1) of Embodiments 1-4,

Comparative example 1 and Comparative example 2 in Shanyang Village, Jiashi Town, Yanhe

County, Yangxi Village, Banchang Town, and the Lentinus edodes base of Chengyao Company,

Bijiang District, respectively. Each treatment is marked and hanged with a yellow card to stick insects, and the number of mosquitoes, moths and red spiders are recorded. The results are shown in Table 4.

Table 4 Effect of insect repellent on preventing rotten sticks of Lentinus edodes LU503155

Number Shanyang Village, Jiashi Yangxi Village, Jiashi Daao Village, Bijiang

Town Town District

Embodime 7/1 13 6.25 7/5 19 15 7/10 21 18 nt 1

Embodime 7/1 15 6.25 7/5 23 16 7/10 24 20 nt 2

Embodime 7/1 19 8.00 7/5 25 12 7/10 23 16 nt 3

Embodime 7/1 14 8.82 7/5 24 14 7/10 26 19 nt 4

Comparativ 7/1 25 17.57 7/5 29 45 7/10 34 28 e example 1

Comparativ 7/1 41 17.65 7/5 55 51 7/10 30 e example 2

In Table 4, the investigation results of Lentinus edodes show that Embodiments 1-4 have a good repellent effect on mosquitoes, flies, noctuids and red spiders of Lentinus edodes. The insect repellent of Embodiments 1-4 combined with spraying water in greenhouse and other cooling measures in high temperature period reduce the rotten rate of Lentinus edodes in summer.

Experiment 3 LUS03155

The chemicals of Embodiments 1-4, Comparative example 1 and Comparative example 2 (the treatment method of chemicals is the same as that of Experiment 1) were sprayed on

Agrocybe aegerita and Flammulina velutipes from March 2021 to October 2021, and the indexes such as armyworm number, rotten stick number and average yield were tested. The armyworm collected after treatment was the average armyworm number five days before spraying, and the number of sticks tested by Agrocybe aegerita and Flammulina velutipes was 25. The results are shown in Table 5:

Agrocybe aegerita and Flammulina velutipes from March 2021 to October 2021, and the indexes such as the number of stuck insects, the number of rotten sticks and average yield are tested. The stuck insects collected after treatment are the average stuck insects number five days before spraying, and the number of sticks tested by Agrocybe aegerita and Flammulina velutipes is 25.

The results are shown in Table 5: LU503155

The The Average The number The Average number of | number yield(kg) of stuck number | yield(kg) stuck of rotten insects(head) | of rotten insects(he | sticks(pi sticks(pi ad) ece) ece)

Embodiment 56 2 0.59 59 2 0.71 1

Embodiment 48 5 0.64 55 4 0.73 2

Embodiment 46 3 0.68 27 3 0.68 3

Embodiment 65 4 0.65 39 3 0.65 4

Comparative 153 13 0.58 125 0.56 example 1

Comparative 156 12 0.46 156 11 0.51 example 2

The results of Table 5 show that Embodiments 1-4 have a better repellent effect on

Agrocybe aegerita and Flammulina velutipes compared with the control insect-proof agent and water. Compared with the traditional insect-proof agent, the number of rotten sticks of mycelia 1s reduced and the yield is increased.

Experiment 4

According to the National Food Safety Standard GB2763-2019 Maximum Residue Limit

Standard of Pesticides in Food, the Pleurotus geesteranus treated by Embodiments 1-4,

Comparative example 1 and Comparative example 2 in Experiment 1 is tested for multiple pesticide residues by the high performance liquid chromatograph.

The test method is: weigh 10.00g of samples and crush them evenly. After adding the samples into a 50ml centrifuge tube, add 10mL of acetonitrile, and swirl for 3 minutes, add 0.5g of disodium citrate, 4g of anhydrous magnesium sulfate, 1g of disodium hydrogen citrate and 145031 55 of sodium chloride into the centrifuge tube, then swirl for 3 minutes, and centrifuge at 8000r/min for 5 minutes, and suck 6mL of acetonitrile layer into a 15mL centrifuge tube.

The purification method is: suck 1.5mL of the extract into the centrifuge tube, add 100mg of anhydrous magnesium sulfate and 100mg of PSA(N-propyl ethylenediamine), swirl for 2min, centrifuge at 8000r/min for Smin, and filter the supernatant with a 0.22um filter membrane.

Use methanol, water and acetonitrile as mobile phase, the ratio of them is 55% methanol, 25% water and 20% acetonitrile, the flow rate is 0.3ml/min, the injection volume is 10uL, and the temperature of chromatographic column is 40°C.

According to the indexes specified in GB2763-2019 Maximum Residue Limit of Pesticides in Food, the detected limit meets the national standards. If it exceeds the detection limit, it can be judged as "detected"; if it exceeds the maximum pesticide residue limit standard, it will be judged as "exceeding the standard"; if there is no prescribed limit, it will not be judged.

The test results are shown in Table 6: LU503155

Carbendazi 0.35 0.26 0.38 0.27 2.690 m

Chlorothalon 0.33 0.25 0.30 0.36 2.34 0.49 il ove | oo [on | 008 | ow | om | em

Cypermethri 0.012 0.025 0.015 0.026 0.36 0.16 n

The results in Table 6 show that Pleurotus geesteranus treated in Embodiments 1-4 meets the national standard, and all the indexes of pesticide residues in Comparative example 1 are beyond the standard range. The pesticide residue content of Pleurotus geesteranus treated in

Embodiments 1-4 is small, which 1s due to the use of pesticides in the cultivation raw materials of edible fungi. The pesticide residue index of Comparative example 2 directly sprayed with water is also close to the standard value, which indicates that there are pesticide residues in the original cultivated fungus sticks, resulting in a small amount of pesticide residues in

Embodiments 1-4, which is much lower than the national standard and meets the food safety requirements.

To sum up, the insect repellent of Embodiments 1-4 can achieve the same insect repellent effect as the commercially available insect-proof agent. Compared with the commercially available products, Embodiments 1-4 can reduce the rotten rate of sticks in Pleurotus geesteranus, Lentinus edodes, Agrocybe aegerita and Flammulina velutipes, thus increasing their yield. Moreover, the edible fungi treated by Embodiments 1-4 can effectively reduce thé:}503155 pesticide residues and improve food safety.

The above is only a detailed illustration and description of the embodiments of the present invention, and is not intended to limit the present invention. For those skilled in the field, the present invention can be modified and varied. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims

CLAIMS LU503155

1. A plant essential oil insect repellent for edible fungi is characterized in that the insect repellent consists of the following components in parts by weight: 12-18 parts of peppermint oil, 15-20 parts of navel orange oil, 20-35 parts of lemon oil, 8-15 parts of citronella oil, 13-20 parts of scutellaria baicalensis extract, 3-8 parts of tobacco leaves, 6-10 parts of stemona and 10-20 parts of emulsifier.

2. The plant essential oil insect repellent for edible fungi according to claim 1 is characterized in that the emulsifier is made of tween -20 and/or octylphenol polyoxyethylene ether.

3. The plant essential oil insect repellent for edible fungi according to claim 1 is characterized in that the insect repellent consists of the following components in parts by weight: parts of peppermint oil, 18 parts of navel orange oil, 25 parts of lemon oil, 10 parts of citronella oil, 15 parts of scutellaria baicalensis extract, 5 parts of tobacco leaves, 8 parts of stemona and 15 parts of emulsifier.

4. The plant essential oil insect repellent for edible fungi according to claim 1 is characterized in that the insect repellent consists of the following components in parts by weight: 16 parts of peppermint oil, 17 parts of navel orange oil, 30 parts of lemon oil, 12 parts of citronella oil, 18 parts of scutellaria baicalensis extract, 6 parts of tobacco leaves, 7 parts of stemona and 13 parts of emulsifier.

5. A preparation method of the plant essential oil insect repellent for edible fungi according to claim 1 is characterized in that it comprises the following steps: (1) weigh each component according to that weight parts of each raw material for later use; (2) respectively crush the tobacco leaves and the stemona into 50-100 meshes particles; mix the crushed tobacco with the mashed stemona, add water and soak in water for 15-20min, the added water is 1-3 times of the total mass of tobacco leaves and stemona, distill, and collect distillate to obtain liquid medicine for later use; (3) mix peppermint oil, navel orange oil, lemon oil and citronella oil, add emulsifier, seal, heat and stir for 25-40min, and control the temperature at 30-40°C;

(4) after mixing the materials in step (2) and step (3) and scutellaria baicalensis extradtJ203155 place the mixture in a distiller and inject steam with a pressure of 90-150MPa; and control the temperature of steam at 100-120°C; (5) stir the liquid in step (4) in a homogenizer, keep the temperature at 60-70°C and stir for half an hour, and cool it to room temperature to obtain the finished product.

6. The preparation of the plant essential oil insect repellent for edible fungi according to claim 5 is characterized in that the stirring speed of the homogenizer is 20-50m/s.

7. Application of the insect repellent according to any one of claims 1-4 in medicine for preventing and controlling the drosophila melanogaster of Pleurotus geesteranus and Lentinus edodes.

8. Application of the insect repellent according to any one of claims 1-4 in the medicine for preventing and controlling pests of Agrocybe aegerita and Flammulina velutipes.