KR980002268A - A maltogenic amylase gene, a recombinant molecule containing it, a transformant having the same, and an enzyme produced therefrom - Google Patents
A maltogenic amylase gene, a recombinant molecule containing it, a transformant having the same, and an enzyme produced therefrom Download PDFInfo
- Publication number
- KR980002268A KR980002268A KR1019960025135A KR19960025135A KR980002268A KR 980002268 A KR980002268 A KR 980002268A KR 1019960025135 A KR1019960025135 A KR 1019960025135A KR 19960025135 A KR19960025135 A KR 19960025135A KR 980002268 A KR980002268 A KR 980002268A
- Authority
- KR
- South Korea
- Prior art keywords
- maltose
- gene
- transformant
- decomposes
- recombinant molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010061330 glucan 1,4-alpha-maltohydrolase Proteins 0.000 title abstract description 3
- 108090000790 Enzymes Proteins 0.000 title abstract 2
- 102000004190 Enzymes Human genes 0.000 title abstract 2
- 108700026220 vif Genes Proteins 0.000 title abstract 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims abstract description 12
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims abstract description 12
- 108010065511 Amylases Proteins 0.000 claims abstract description 9
- 239000004382 Amylase Substances 0.000 claims abstract description 7
- 102000013142 Amylases Human genes 0.000 claims abstract description 7
- 235000019418 amylase Nutrition 0.000 claims abstract description 7
- 229920000858 Cyclodextrin Polymers 0.000 claims abstract description 5
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 5
- 229920002472 Starch Polymers 0.000 claims abstract description 4
- 239000008107 starch Substances 0.000 claims abstract description 4
- 235000019698 starch Nutrition 0.000 claims abstract description 4
- 238000001155 isoelectric focusing Methods 0.000 claims abstract 5
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims abstract 3
- 241000193385 Geobacillus stearothermophilus Species 0.000 claims abstract 2
- 230000002068 genetic effect Effects 0.000 claims abstract 2
- 229920001218 Pullulan Polymers 0.000 claims description 2
- 239000004373 Pullulan Substances 0.000 claims description 2
- 235000019423 pullulan Nutrition 0.000 claims description 2
- FWQHNLCNFPYBCA-UHFFFAOYSA-N fluoran Chemical compound C12=CC=CC=C2OC2=CC=CC=C2C11OC(=O)C2=CC=CC=C21 FWQHNLCNFPYBCA-UHFFFAOYSA-N 0.000 abstract 1
- 239000001116 FEMA 4028 Substances 0.000 description 2
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 description 2
- 235000011175 beta-cyclodextrine Nutrition 0.000 description 2
- 229960004853 betadex Drugs 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
본 발명은 Bacillus Stearothermophilus KCTC1044BP로부터 분리한 말토오스생성 아밀라제 유전자로, 제1도에 표시된 바와 같은 유전자 지도를 가지며, 10% SDS-PAGE로 측정한 분자량이 약 62000 Dalton이고, 아이소일렉트릭 포커싱 (isoelectric focusing: IEF)에 의해 측정한 등전점이 약 4.0이고, 상기 유전자에 의해 생성된 말토오스생성 아밀라제는 플루란에 작용하여 주로 판노오스를 생성하고, 전분을 분해하여 주로 말토오스를 생성하며, β-사이클로덱스트린을 분해하는, 말토오스생성 아밀라제 유전자에 관한 것이다. 또한 본 발명은 상기 유전자를 포함하는 재조합 분자, 그것을 가지는 형질전환체 및 그것으로부터 생산되는 효소에 관한 것이다.The present invention relates to a maltose-producing amylase gene isolated from Bacillus stearothermophilus KCTC1044BP, which has a genetic map as shown in FIG. 1 and has a molecular weight of about 62000 Dalton as measured by 10% SDS-PAGE and isoelectric focusing (IEF ), The maltose-producing amylase produced by this gene acts on fluoran to produce mainly pannocose, decomposes starch to mainly produce maltose, and decomposes? -Cyclodextrin , And a maltogenic amylase gene. The present invention also relates to a recombinant molecule comprising said gene, a transformant having said recombinant molecule, and an enzyme produced therefrom.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is a trivial issue, I did not include the contents of the text.
제1도는 본 발명의 말토오스생성 아밀라제의 유전자 제한지도이다.Figure 1 is a gene restriction map of the maltose-producing amylase of the present invention.
제2도는 본 발명의 말토오스생성 아밀라제의 분자량 전기영동 사진이다.FIG. 2 is a molecular weight electrophoresis photograph of the maltose-producing amylase of the present invention.
제4도는 β-사이클로덱스트린을 기질로 하였을 때 본 발명의 말토오스생성 아밀라제의 온도 특성곡선이다.FIG. 4 is a temperature characteristic curve of the maltose-producing amylase of the present invention when β-cyclodextrin is used as a substrate.
제6도는 풀루란, 전분, β-사이클로덱스트린에 대한, 본 발명 말토오스생성 아밀라제의 시간별 작용형태를 보여주는 박충크로마토그래피이다.FIG. 6 is a buccal chromatogram showing the time course of the maltogenic amylase of the present invention against pullulan, starch, β-cyclodextrin.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019960025135A KR100186716B1 (en) | 1996-06-28 | 1996-06-28 | Maltose-producing amylases, recombinant vectors comprising the genes encoding them and transformants thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019960025135A KR100186716B1 (en) | 1996-06-28 | 1996-06-28 | Maltose-producing amylases, recombinant vectors comprising the genes encoding them and transformants thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR980002268A true KR980002268A (en) | 1998-03-30 |
| KR100186716B1 KR100186716B1 (en) | 1999-04-01 |
Family
ID=19464296
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019960025135A Expired - Fee Related KR100186716B1 (en) | 1996-06-28 | 1996-06-28 | Maltose-producing amylases, recombinant vectors comprising the genes encoding them and transformants thereof |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR100186716B1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100625100B1 (en) * | 2002-07-15 | 2006-09-19 | 주식회사 제노포커스 | Method for producing maltopentaose producing amylase and maltopentaose |
| KR20170040634A (en) | 2015-10-05 | 2017-04-13 | 한림대학교 산학협력단 | Method for production of maltose with novel enzyme |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109486791B (en) * | 2018-11-22 | 2020-11-03 | 湖南汇升生物科技有限公司 | Preparation and application of maltogenic amylase mutant |
| CN109370973B (en) * | 2018-11-22 | 2020-12-29 | 湖南汇升生物科技有限公司 | Maltogenic amylase producing strain |
-
1996
- 1996-06-28 KR KR1019960025135A patent/KR100186716B1/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100625100B1 (en) * | 2002-07-15 | 2006-09-19 | 주식회사 제노포커스 | Method for producing maltopentaose producing amylase and maltopentaose |
| KR20170040634A (en) | 2015-10-05 | 2017-04-13 | 한림대학교 산학협력단 | Method for production of maltose with novel enzyme |
Also Published As
| Publication number | Publication date |
|---|---|
| KR100186716B1 (en) | 1999-04-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX9306955A (en) | AEROSOL SPRAY FORMULATIONS FOR HAIR. | |
| DE60032367D1 (en) | ACTIVE RECOMBINANT NEUROTOXINE | |
| DE69131580D1 (en) | GDF-1 and UOG1 proteins | |
| DE69720979D1 (en) | THERMOSTABILIZED CONTRAST AGENT | |
| DE69936778D1 (en) | ADENOASSOZED VIRUS VECTORS AND ITS USES | |
| DE60141546D1 (en) | Polypeptides containing the extracellular domain of IL-20RA and / or IL-20RB | |
| DK1062936T3 (en) | Cosmetic composition for the hair in the form of a water-in-silicone emulsion comprising at least one fixative polymer | |
| NO971875D0 (en) | Cathepsin 02 protease | |
| FR2774588B1 (en) | COSMETIC OR DERMATOLOGICAL COMPOSITION CONTAINING AT LEAST ONE NATURAL, RECOMBINANT ARACHNID SILK PROTEIN OR THE LIKE | |
| TR200102740T2 (en) | Azeotrope-like compositions containing 1,1,1,3,3-Pentafluorobutane. | |
| DK0605040T3 (en) | Pullulanase, microorganisms producing this process for the preparation of this pullulanase and its use | |
| TR199900267T2 (en) | GnRH-L�kotoksin kimeralar�. | |
| KR920000967A (en) | Method of Forming Silicon Nitride Film | |
| EE9900581A (en) | LAG-3 pleisimisteisendid | |
| KR950005319A (en) | Pharmaceutical composition | |
| AU3953685A (en) | Crosslinkable polysiloxane composition | |
| EP0934946A3 (en) | Methods of making polysiloxanes | |
| ATE199377T1 (en) | PROTEIN WITH CYTOKINE ACTIVITY, RECOMBINANT DNA, EXPRESSION VECTOR AND HOST CELLS FOR ITS PRODUCTION | |
| KR980002268A (en) | A maltogenic amylase gene, a recombinant molecule containing it, a transformant having the same, and an enzyme produced therefrom | |
| FI972384A7 (en) | Novel dipeptide p-amidinobenzylamides containing N-terminal sulfonyl or amidinosulfonyl groups | |
| TR199903014T2 (en) | Carboxamides useful as 5-HT1F Agonists. | |
| EP1064296A4 (en) | Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus | |
| WO1998039450A3 (en) | Surface protein (spsa protein) of streptococcus pneumoniae, deleted derivatives, expression system for said proteins and vaccine system with said proteins | |
| TR199901190T2 (en) | Methods and compositions for the delivery and synthesis of interferon-(alpha) nucleic acids. | |
| FR2692282B1 (en) | CLONING OF THE GENE ENCODING THE PROTEIN GP28.5 OF T. GONDII; PEPTIDE FRAGMENTS FROM SUCH PROTEIN AND THEIR APPLICATIONS. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| PA0109 | Patent application |
Patent event code: PA01091R01D Comment text: Patent Application Patent event date: 19960628 |
|
| PA0201 | Request for examination |
Patent event code: PA02012R01D Patent event date: 19960628 Comment text: Request for Examination of Application |
|
| PG1501 | Laying open of application | ||
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 19980825 Patent event code: PE09021S01D |
|
| E701 | Decision to grant or registration of patent right | ||
| PE0701 | Decision of registration |
Patent event code: PE07011S01D Comment text: Decision to Grant Registration Patent event date: 19981125 |
|
| GRNT | Written decision to grant | ||
| PR0701 | Registration of establishment |
Comment text: Registration of Establishment Patent event date: 19981229 Patent event code: PR07011E01D |
|
| PR1002 | Payment of registration fee |
Payment date: 19981229 End annual number: 3 Start annual number: 1 |
|
| PG1601 | Publication of registration | ||
| PR1001 | Payment of annual fee |
Payment date: 20010209 Start annual number: 4 End annual number: 6 |
|
| PR1001 | Payment of annual fee |
Payment date: 20041206 Start annual number: 7 End annual number: 7 |
|
| PR1001 | Payment of annual fee |
Payment date: 20051205 Start annual number: 8 End annual number: 8 |
|
| PR1001 | Payment of annual fee |
Payment date: 20061204 Start annual number: 9 End annual number: 9 |
|
| PR1001 | Payment of annual fee |
Payment date: 20071203 Start annual number: 10 End annual number: 10 |
|
| PR1001 | Payment of annual fee |
Payment date: 20081201 Start annual number: 11 End annual number: 11 |
|
| PR1001 | Payment of annual fee |
Payment date: 20091202 Start annual number: 12 End annual number: 12 |
|
| PR1001 | Payment of annual fee |
Payment date: 20101201 Start annual number: 13 End annual number: 13 |
|
| PR1001 | Payment of annual fee |
Payment date: 20120629 Start annual number: 14 End annual number: 14 |
|
| FPAY | Annual fee payment |
Payment date: 20130130 Year of fee payment: 15 |
|
| PR1001 | Payment of annual fee |
Payment date: 20130130 Start annual number: 15 End annual number: 15 |
|
| LAPS | Lapse due to unpaid annual fee | ||
| PC1903 | Unpaid annual fee |
Termination category: Default of registration fee Termination date: 20141109 |