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KR970066561A - Biosensor for measuring glucose and method for producing the same - Google Patents

Biosensor for measuring glucose and method for producing the same Download PDF

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Publication number
KR970066561A
KR970066561A KR1019960007178A KR19960007178A KR970066561A KR 970066561 A KR970066561 A KR 970066561A KR 1019960007178 A KR1019960007178 A KR 1019960007178A KR 19960007178 A KR19960007178 A KR 19960007178A KR 970066561 A KR970066561 A KR 970066561A
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South Korea
Prior art keywords
biosensor
enzyme
electrode
electron mediator
paste
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KR1019960007178A
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Korean (ko)
Inventor
김인수
신민철
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성재갑
주식회사 Lg 화학
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Priority to KR1019960007178A priority Critical patent/KR970066561A/en
Publication of KR970066561A publication Critical patent/KR970066561A/en

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  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 글루코스 측정용 바이오센서 및 그의 제작 방법에 관한 것으로, 절연성 기판; 절연성 기판 상에 위치하는 효소 지시 전극, 비효소 지시 전극 및 기준 전극으로 이루어진 전극계; 및 전극계 상부에 위치하는 모세관 시료 수용부를 포함하는 스트립 형태의 글루코스 측정용 바이오센서는 간섭 신호를 배제할 수 있고 차동증폭형 측정이 가능하고 대량생산이 가능하다.The present invention relates to a biosensor for measuring glucose and a method of manufacturing the same, and more particularly, An electrode system comprising an enzyme indicating electrode, a non-enzyme indicating electrode, and a reference electrode located on an insulating substrate; And a capillary sample accommodating unit located at the upper part of the electrode system, can eliminate an interference signal and can perform differential amplification measurement and mass production.

Description

글루코스 측정용 바이오센서 및 그의 제작 방법Biosensor for measuring glucose and method for producing the same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is a trivial issue, I did not include the contents of the text.

제1도는 본 발명의 바아오센서의 사시도이고, 제2도는 본 발명의 바이오센서의 제작 단계를 나타낸 것이고, 제3도는 본 발명의 바이오센서에서 글루코스 농도의 변화에 따른 전류 변화를 나타낸 그래프이다.FIG. 1 is a perspective view of a biosensor according to the present invention, FIG. 2 is a diagram showing a manufacturing step of a biosensor according to the present invention, and FIG. 3 is a graph showing a current change according to a change in glucose concentration in the biosensor of the present invention.

Claims (11)

절연성 기판; 절연성 기판 상에 위치하는 효소 지시 전극, 비효소 지시 전극 및 기준 전극으로 이루어진 전극계; 및 전극계 상부에 위치하는 모세관 시료 수용부를 포함하는 스트립 형태의 글루코스 측정용 바이오센서.An insulating substrate; An electrode system comprising an enzyme indicating electrode, a non-enzyme indicating electrode, and a reference electrode located on an insulating substrate; And a capillary sample accommodating portion located at an upper portion of the electrode system. 제1항에 있어서, 상기 절연성 기판이 폴리에서테르, PVC 또는 폴리카보네이트로 이루어진 것을 특징으로 하는 글루코스 측정용 바이오센서.The biosensor for glucose measurement according to claim 1, wherein the insulating substrate is made of poly, terpolymer, PVC or polycarbonate. 제1항에 있어서, 상기 효소 지시 전극이 효소, 고분자 물질 및 전자매개체가 흡착된 탄소를 포함하는 페이스트를 인쇄하여 형성되는 것을 특징으로 하는 글루코스 측정용 바이오센서.The biosensor for glucose measurement according to claim 1, wherein the enzyme indicating electrode is formed by printing a paste containing an enzyme, a polymer substance and carbon adsorbed on the electron mediator. 제3항에 있어서, 상기 효소가 글루코스 산화 효소(glucose oxidase)인 것을 특징으로 하는 글루코스 측정용 바이오센서.4. The biosensor for measuring glucose according to claim 3, wherein the enzyme is glucose oxidase. 제1항에 있어서, 상기 비효소 지시 전극이 비효소 단백질, 고분자 물질 및 전자매개체가 흡착된 탄소를 포함하는 페이스트를 인쇄하여 형성되는 것을 특징으로 하는 글루코스 측정용 바이오센서.The biosensor for measuring glucose according to claim 1, wherein the non-enzyme indicating electrode is formed by printing a paste containing non-enzyme protein, polymer material and carbon adsorbed on the electron mediator. 제5항에 있어서, 상기 비효소 단백질이 소혈청알부민(bovine serum albumin; BSA)인 것을 특징으로 하는 글루코스 측정용 바이오센서.6. The biosensor for measuring glucose according to claim 5, wherein the non-enzyme protein is bovine serum albumin (BSA). 제3항 또는 제5항에 있어서, 상기 고분자 물질이 히드록시에틸 셀룰로스 또는 폴리비닐피롤리돈인 것을 특징으로 하는 글루코스 측정용 바이오센서.The biosensor for measuring glucose according to claim 3 or 5, wherein the polymer substance is hydroxyethyl cellulose or polyvinyl pyrrolidone. 제3항 또는 제5항에 있어서, 상기 전자매개체가 페로신(ferrocene), 페로신 모노카르복실산, 디메틸 페로신 또는 페리시아니드(ferricyanide)인 것을 특징으로 하는 글루코스 측정용 바이오센서.The biosensor for glucose measurement according to claim 3 or 5, wherein the electron mediator is ferrocene, ferrocine monocarboxylic acid, dimethylferrocin or ferricyanide. 제3항 또는 제5항에 있어서, 상기 전자매개체가 흡착된 탄소는 탄소 1g당 전자매개체 10 내지 60㎎이 흡착된 것임을 특징으로 하는 글루코스 측정용 바이오센서.The biosensor for measuring glucose according to claim 3 or 5, wherein the carbon adsorbed on the electron mediator is adsorbed with 10 to 60 mg of an electron mediator per 1 g of carbon. 제1항에 있어서, 상기 모세관 시료 수용부가 시료 투입 구멍이 있는 외부 보호층과, 이를 전극계와 접착 시키는 시료 투입 가이드층으로 되는 것을 특징으로 하는 글루코스 측정용 바이오센서.The biosensor for glucose measurement according to claim 1, wherein the capillary sample accommodating portion comprises an outer protective layer having a sample injection hole and a sample introduction guide layer for adhering the outer protective layer to the electrode system. 절연성 기판 위에 은 페이스트를 인쇄한 다음 열처리하는 단계; 탄소페이스트를 인쇄한 다음 열처리하는 단계; 절연 페이스트를 인쇄한 다음 열처리하는 단계; FeCl3용액으로 처리하여 의사 Ag/AgCl 기준 전극을 형성하는 단계; 효소, 고분자 물질 및 전자 매개체가 흡착된 탄소를 포함하는 페이스트 및 비효소 단백질, 고분자 물질 및 전자매개체가 흡착된 탄소를 포함하는 페이스트를 스크린 인쇄하는 단계; 및 모세관 시료 수용부를 접착시키는 단계를 포함하는 글루코스 측정용 바이오센서의 제작 방법.Printing a silver paste on an insulating substrate and then performing heat treatment; Printing and then heat treating the carbon paste; Printing and then heat-treating the insulating paste; FeCl 3 solution to form a pseudo Ag / AgCl reference electrode; Screen printing a paste containing paste and non-enzyme protein, polymer material and carbon adsorbed on the electron mediator, the paste including an enzyme, a polymer substance and carbon adsorbed on the electron mediator; And adhering the capillary sample accommodating portion to the capillary sample receiving portion. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: It is disclosed by the contents of the first application.
KR1019960007178A 1996-03-18 1996-03-18 Biosensor for measuring glucose and method for producing the same KR970066561A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000060340A1 (en) * 1999-04-06 2000-10-12 Allmedicus Corporation Electrochemical biosensor test strip, fabrication method thereof and electrochemical biosensor
KR20040007028A (en) * 2002-07-16 2004-01-24 라이프스캔, 인코포레이티드 Electrochemical test strip having a plurality of reaction chambers and methods for using the same
KR101135624B1 (en) * 2009-01-15 2012-04-17 한양대학교 산학협력단 A biosensor coated with electroactive polymer layer for extension of biosensor life span

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07209242A (en) * 1992-08-28 1995-08-11 Nec Corp Biosensor and manufacture and using method thereof
JPH07325064A (en) * 1994-06-02 1995-12-12 Matsushita Electric Ind Co Ltd Manufacture of biosensor

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07209242A (en) * 1992-08-28 1995-08-11 Nec Corp Biosensor and manufacture and using method thereof
JPH07325064A (en) * 1994-06-02 1995-12-12 Matsushita Electric Ind Co Ltd Manufacture of biosensor

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000060340A1 (en) * 1999-04-06 2000-10-12 Allmedicus Corporation Electrochemical biosensor test strip, fabrication method thereof and electrochemical biosensor
US6805780B1 (en) 1999-04-06 2004-10-19 Allmedicus Co., Ltd. Electrochemical biosensor test strip, fabrication method thereof and electrochemical biosensor
KR20040007028A (en) * 2002-07-16 2004-01-24 라이프스캔, 인코포레이티드 Electrochemical test strip having a plurality of reaction chambers and methods for using the same
KR101135624B1 (en) * 2009-01-15 2012-04-17 한양대학교 산학협력단 A biosensor coated with electroactive polymer layer for extension of biosensor life span

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