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KR970048455A - 매독 트레포네마 융합 항원 및 그 융합 항원을 사용한 항매독 트레포네마 항체의 측정 방법 - Google Patents

매독 트레포네마 융합 항원 및 그 융합 항원을 사용한 항매독 트레포네마 항체의 측정 방법 Download PDF

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Publication number
KR970048455A
KR970048455A KR1019960071472A KR19960071472A KR970048455A KR 970048455 A KR970048455 A KR 970048455A KR 1019960071472 A KR1019960071472 A KR 1019960071472A KR 19960071472 A KR19960071472 A KR 19960071472A KR 970048455 A KR970048455 A KR 970048455A
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Prior art keywords
fusion antigen
syphilis treponema
15kda
fusion
order
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KR1019960071472A
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KR100257574B1 (ko
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노부유끼 이세
다께야 호리
가쯔야 후지무라
테쯔지 다니모또
마사히사 오까다
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원본미기재
후지레비오 가부시끼가이샤
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/571—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses for venereal disease, e.g. syphilis, gonorrhoea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/20—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Spirochaetales (O), e.g. Treponema, Leptospira
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/806—Antigenic peptides or proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
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  • Veterinary Medicine (AREA)
  • Hematology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
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  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 매독 트레포네마 융합 항원 및 그 융합 항원을 사용한 항매독 트레포네마 항체의 측정 방법에 관한 것이다. 본 발명에 의해 매독 트레포네마의 표면항원이 2개 이상 융합된 매독 트레포네마 융합 항원이 제공된다. 본 발명에 따른 융합 항원은 각 표면 항원을 각각 단독으로 사용한 경우보다 훨씬 항 Tp 항체와의 반응성이 높고, 그 융합 항원을 항 Tp 항체의 측정계에 사용함으로서, 종래에는 없는 높은 측정 감도를 가진항 Tp 항체 측정 방법이 제공될 수 있다.

Description

매독 트레포네마 융합 항원 및 그 융합 항원을 사용한 항매독 트레포네마 항체의 측정 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 발현 벡터 pW6A의 상세 구조를 나타낸 도면,
제2도는 47K 항체를 코드하는 유전자의 조제 절차를 나타낸 도면.

Claims (6)

  1. 매독 트레포네마의 표면 항원이 2개 이상 융합된 매독 트레포네마 융합 항원.
  2. 제1항에 있어서, 매독 트레포네마의 표면 항원이 3개 이상 융합된 매독 트레포네마 융합 항원.
  3. 제1항에 있어서, 매독 트레포네마의 표면 항원이 2 내지 3개 융합된 매독 트레포네마 융합 항원.
  4. 제1항 내지 제3항중 어느 한 항에 있어서, 매독 트레포네마의 표면 항원의 분자량이 47kDa, 17kDa, 15kDa인 매독 트레포네마 융합 항원.
  5. 제4항에 있어서, 매독 트레포네마 융합 항원이 N 말단측에서 C말단으로, 47kDa, 17kDa, 15kD의 순으로 융합된 융합 항원, 47kDa,, 15kDa,17kDa의 순으로 융합된 융합 항원, 15kDa,17kDa,47kDa순으로 융합된 융합항원, 17kDa,47kDa,15kDa,순으로 융합된 융합 항원 47kDa,17kDa,의 순으로 융합된 융합 항원, 47kDa,15kDa의 순으로 융합된 융합 항원, 17kDa,47kDa의 순으로 융합된 융합 항원, 17kDa,15kDa,의 순으로 융합된 융합 항원, 15kDa,47kDa의 순으로 융합된 융합 항원, 15kDa,17kDa의 순으로 융합된 융합 항원, 15kDa,15kDa의 순으로 융합된 융합 항원, 15kDa,15kDa,15kDa의 순으로 융합된 융합 항원, 15kDa, 15kDa,15kDa,15kDa의 순으로 융합된 융합 항원인 융합 항원.
  6. 제1항 내지 제5항에 기재된 매독 트레포네마 융합 항원을 사용한 항매독 트레포네마 항체의 측정 방법.
    ※ 참고사항: 최초출원 내용에 의하여 공개하는 것임.
KR1019960071472A 1995-12-25 1996-12-24 매독 트레포네마 융합 항원 및 그 융합 항원을 사용한 항매독 트레포네마 항체의 측정 방법 Expired - Lifetime KR100257574B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP35007295 1995-12-25
JP95-350072 1995-12-25

Publications (2)

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KR970048455A true KR970048455A (ko) 1997-07-29
KR100257574B1 KR100257574B1 (ko) 2000-08-01

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KR1019960071472A Expired - Lifetime KR100257574B1 (ko) 1995-12-25 1996-12-24 매독 트레포네마 융합 항원 및 그 융합 항원을 사용한 항매독 트레포네마 항체의 측정 방법

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US (2) US6248331B1 (ko)
EP (1) EP0781846B1 (ko)
KR (1) KR100257574B1 (ko)
AT (1) ATE250128T1 (ko)
CA (1) CA2193870C (ko)
DE (1) DE69630008T2 (ko)
ES (1) ES2207671T3 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20140108730A (ko) * 2012-01-19 2014-09-12 에프. 호프만-라 로슈 아게 가용성 면역반응성 트레포네마 팔리둠 ｔｐｎ４７ 항원

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EP0985931A3 (en) * 1998-09-04 2000-03-29 Becton Dickinson and Company Recombinant antigen immunoassay for the diagnosis of syphilis
US20050153382A1 (en) * 2002-06-06 2005-07-14 Chengdu Kuachang Science And Technology Co., Ltd. Biochip kit comprising biochip based on antigen-antibody reactions, and its usage
ES2405848T3 (es) * 2003-12-23 2013-06-04 Biokit S.A. Composiciones y métodos para detección de infección patógena
US20060234321A1 (en) * 2005-04-14 2006-10-19 University Of Washington Syphilis diagnostic tests and kits
US20100003706A1 (en) * 2005-04-14 2010-01-07 Washington, University Of Syphilis diagnostic tests and kits
CN101858916A (zh) * 2010-05-19 2010-10-13 厦门大学附属中山医院 梅毒特异性IgM抗体胶体金免疫层析检测试剂条及其制备方法
CN101858915A (zh) * 2010-05-19 2010-10-13 厦门大学附属中山医院 梅毒特异性IgG抗体胶体金免疫层析检测试剂条及其制备方法
CN108085330A (zh) * 2011-01-13 2018-05-29 奥索临床诊断有限公司 苍白密螺旋体三联抗原
ES2834880T3 (es) 2015-03-10 2021-06-21 Bio Rad Laboratories Prueba combinada de sífilis treponémica y no treponémica
CN111116718A (zh) * 2020-02-17 2020-05-08 北京博生福生物技术有限责任公司 一种蛋白连接肽及其在改造tp重组抗原中的应用
WO2022070423A1 (ja) * 2020-10-02 2022-04-07 オリンパス株式会社 情報処理装置、情報処理装置の作動方法、及びプログラム

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EP0126765A1 (en) 1982-11-12 1984-12-05 The Regents Of The University Of California Recombinant dna derived antigens of treponema pallidum
US4976958A (en) * 1987-02-26 1990-12-11 Scripps Clinic And Research Foundation Mycobacterial recombinants and peptides
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JP3053720B2 (ja) * 1993-08-24 2000-06-19 株式会社竹中工務店 大空間施設の気流発生装置
ATE263836T1 (de) * 1993-11-01 2004-04-15 Brookhaven Science Ass Llc Chimere proteine, welche borrelia polypeptide beinhalten, verwendungen dafür
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20140108730A (ko) * 2012-01-19 2014-09-12 에프. 호프만-라 로슈 아게 가용성 면역반응성 트레포네마 팔리둠 ｔｐｎ４７ 항원

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EP0781846A3 (en) 1998-05-13
CA2193870C (en) 2000-07-18
EP0781846A2 (en) 1997-07-02
US6248331B1 (en) 2001-06-19
US20010002311A1 (en) 2001-05-31
KR100257574B1 (ko) 2000-08-01
DE69630008T2 (de) 2004-07-08
ATE250128T1 (de) 2003-10-15
US6794155B2 (en) 2004-09-21
EP0781846B1 (en) 2003-09-17
CA2193870A1 (en) 1997-06-26
ES2207671T3 (es) 2004-06-01
DE69630008D1 (de) 2003-10-23

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