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KR970043052A - Genetically modified plasmid, microorganism and method for preparing glutamic acid using the same - Google Patents

Genetically modified plasmid, microorganism and method for preparing glutamic acid using the same Download PDF

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Publication number
KR970043052A
KR970043052A KR1019950072241A KR19950072241A KR970043052A KR 970043052 A KR970043052 A KR 970043052A KR 1019950072241 A KR1019950072241 A KR 1019950072241A KR 19950072241 A KR19950072241 A KR 19950072241A KR 970043052 A KR970043052 A KR 970043052A
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KR
South Korea
Prior art keywords
glutamic acid
brevibacterium
microorganism
same
genetically modified
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Application number
KR1019950072241A
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Korean (ko)
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KR0185578B1 (en
Inventor
이진호
오윤석
김창겸
심재익
한종권
정성오
전영중
Original Assignee
손경식
제일제당 주식회사
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Priority to KR1019950072241A priority Critical patent/KR0185578B1/en
Publication of KR970043052A publication Critical patent/KR970043052A/en
Application granted granted Critical
Publication of KR0185578B1 publication Critical patent/KR0185578B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01004—Glutamate dehydrogenase (NADP+) (1.4.1.4)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

1. 청구범위에 기재된 발명이 속한 기술분야: 유전공학1. TECHNICAL FIELD OF THE INVENTION

2. 발명이 해결하려고 하는 기술적 과제: 글루타민산의 생산성 증대2. The technical problem the invention seeks to solve: increasing the productivity of glutamic acid

3. 발명의 해결방법의 요지: 브레비박테리움 플라붐으로부터 유래된 글루타메이트 디하이드로게나제 유전자를 함유한 재조합 플라스미드 pGH16으로 형질전환된 브레비박테리움 속 균주3. Summary of Solution of the Invention: Brevibacterium sp. Strain transformed with recombinant plasmid pGH16 containing glutamate dehydrogenase gene derived from Brevibacterium plaboom

4. 발명의 중요한 용도: 글루타민산 제조4. Significant Uses of the Invention: Preparation of glutamic acid

Description

유전자 재조합 플라스미드, 미생물 및 이를 이용한 글루타민산의 제조방법Genetically modified plasmid, microorganism and method for preparing glutamic acid using the same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (5)

브레비박테리움 플라붐을부터 클로닝된 글루타메이트 디하이드로게나제 유전자를 함유한 재조합 플라스미드 pGH16.Recombinant plasmid pGH16 containing glutamate dehydrogenase gene cloned from Brevibacterium flaboom. 제 1항의 재조합 플라스미드 pGH16으로 형질전환된 브레비박테리움 속 균주.Brevibacterium sp. Strain transformed with the recombinant plasmid pGH16 of claim 1. 제 2항에 있어서, 숙주가 브레비박테리움 플라붐인 균주.The strain of claim 2, wherein the host is Brevibacterium flaboom. 제 2항의 형질전환 균주를 배양하여 글루타민산을 제조하는 방법.A method for producing glutamic acid by culturing the transformed strain of claim 2. 브레비박테리움 플라붐으로부터 클로닝된 글루타메이트 디하이드로게나제 유전자.Glutamate dehydrogenase gene cloned from Brevibacterium plaboom.
KR1019950072241A 1995-12-29 1995-12-29 Gene recombination plasmid, micro-organism and preparation process of glutamic acid using it KR0185578B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950072241A KR0185578B1 (en) 1995-12-29 1995-12-29 Gene recombination plasmid, micro-organism and preparation process of glutamic acid using it

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950072241A KR0185578B1 (en) 1995-12-29 1995-12-29 Gene recombination plasmid, micro-organism and preparation process of glutamic acid using it

Publications (2)

Publication Number Publication Date
KR970043052A true KR970043052A (en) 1997-07-26
KR0185578B1 KR0185578B1 (en) 1999-04-01

Family

ID=19448894

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019950072241A KR0185578B1 (en) 1995-12-29 1995-12-29 Gene recombination plasmid, micro-organism and preparation process of glutamic acid using it

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Publication number Publication date
KR0185578B1 (en) 1999-04-01

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